Mutagenetix > Incidental Mutation

Incidental Mutation 'R8530:Vmn2r58'

658990

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r58

Ensembl Gene

ENSMUSG00000090383

Gene Name

vomeronasal 2, receptor 58

Synonyms

EG628422

MMRRC Submission

068500-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.377) question?

Stock #

R8530 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

41486305-41522094 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 41513576 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 356 (W356R)

Ref Sequence

ENSEMBL: ENSMUSP00000126966 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171671]

AlphaFold

K7N6V2

Predicted Effect

probably damaging
Transcript: ENSMUST00000171671
AA Change: W356R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000126966
Gene: ENSMUSG00000090383
AA Change: W356R

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	8e-43	PFAM
Pfam:NCD3G	514	567	1.8e-23	PFAM
Pfam:7tm_3	597	835	2.9e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	G	C	3: 137,774,586 (GRCm39)	E1258D	probably damaging	Het
4930407I10Rik	T	A	15: 81,949,587 (GRCm39)	H1161Q	probably damaging	Het
Adam20	A	G	8: 41,249,071 (GRCm39)	S394G	probably damaging	Het
Akr1c12	A	T	13: 4,320,160 (GRCm39)	F310Y	probably benign	Het
Ank	G	A	15: 27,544,490 (GRCm39)	A84T	probably benign	Het
Bcas1	A	T	2: 170,229,868 (GRCm39)	I244K	probably damaging	Het
Cacna1a	T	C	8: 85,339,043 (GRCm39)		probably null	Het
Car14	T	C	3: 95,807,670 (GRCm39)	H79R	probably benign	Het
Cd163	T	C	6: 124,295,860 (GRCm39)	Y735H	probably damaging	Het
Cdh11	G	A	8: 103,391,387 (GRCm39)	P283L	probably benign	Het
Cfi	C	T	3: 129,644,382 (GRCm39)	T126I	possibly damaging	Het
Col6a4	T	A	9: 105,957,704 (GRCm39)	H40L	probably benign	Het
Dbh	A	G	2: 27,058,318 (GRCm39)	D162G	probably damaging	Het
Dnah14	A	G	1: 181,492,511 (GRCm39)	K1657R	probably damaging	Het
E2f7	T	A	10: 110,614,859 (GRCm39)	V521D	probably benign	Het
Eif3g	A	C	9: 20,809,026 (GRCm39)	S93A	possibly damaging	Het
Fa2h	T	C	8: 112,082,788 (GRCm39)	E143G	probably benign	Het
Foxc1	C	A	13: 31,991,771 (GRCm39)	P194Q	probably benign	Het
Fyco1	A	G	9: 123,669,605 (GRCm39)		probably null	Het
Gabrb3	T	C	7: 57,461,819 (GRCm39)	S256P	probably damaging	Het
Gcc1	C	A	6: 28,420,730 (GRCm39)	D196Y	probably damaging	Het
Herc1	T	A	9: 66,325,910 (GRCm39)	D1461E	probably benign	Het
Hmcn2	C	A	2: 31,281,088 (GRCm39)	L1867I	probably benign	Het
Hps6	A	T	19: 45,991,959 (GRCm39)		probably benign	Het
Macc1	A	T	12: 119,409,474 (GRCm39)	I81F	probably damaging	Het
Man2c1	G	T	9: 57,038,922 (GRCm39)	D111Y	probably damaging	Het
Mast3	A	T	8: 71,240,877 (GRCm39)	I240N	possibly damaging	Het
Matn1	A	T	4: 130,677,447 (GRCm39)	K219*	probably null	Het
Nup210	C	G	6: 91,053,627 (GRCm39)	A297P	possibly damaging	Het
Or5d43	A	T	2: 88,105,154 (GRCm39)	L80I	probably damaging	Het
Or6d12	T	A	6: 116,493,530 (GRCm39)	I264K	probably damaging	Het
Pcare	T	C	17: 72,059,101 (GRCm39)	Y192C	probably damaging	Het
Pcnt	G	A	10: 76,256,039 (GRCm39)	R734W	probably damaging	Het
Phactr3	A	G	2: 177,925,819 (GRCm39)	N360D	probably damaging	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 110,350,827 (GRCm39)		probably benign	Het
Potefam3a	T	C	8: 20,356,984 (GRCm38)	N242S	probably benign	Het
Qtrt2	C	A	16: 43,689,407 (GRCm39)	G197V	probably damaging	Het
Rabep1	A	T	11: 70,810,068 (GRCm39)	L500F	probably damaging	Het
Rhof	T	A	5: 123,257,581 (GRCm39)	D183V	probably damaging	Het
Shprh	T	C	10: 11,027,678 (GRCm39)	V95A	probably benign	Het
Spata31d1b	A	T	13: 59,864,964 (GRCm39)	N704I	unknown	Het
Tas1r2	A	T	4: 139,389,460 (GRCm39)	Y452F	probably benign	Het
Tc2n	A	T	12: 101,617,444 (GRCm39)	F325Y	possibly damaging	Het
Tiam2	G	T	17: 3,501,087 (GRCm39)	V909L	probably benign	Het
Tlx1	A	T	19: 45,139,524 (GRCm39)	Y57F	probably benign	Het
Uck1	C	A	2: 32,150,153 (GRCm39)		probably benign	Het
Wars1	G	A	12: 108,848,818 (GRCm39)	A43V	probably damaging	Het
Wrn	A	T	8: 33,770,852 (GRCm39)	I694N	possibly damaging	Het
Zfp120	A	G	2: 149,959,168 (GRCm39)	Y385H	probably benign	Het

Other mutations in Vmn2r58

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Vmn2r58	APN	7	41,513,854 (GRCm39)	missense	possibly damaging	0.53
IGL00924:Vmn2r58	APN	7	41,486,891 (GRCm39)	missense	probably damaging	1.00
IGL01291:Vmn2r58	APN	7	41,513,935 (GRCm39)	missense	probably benign	0.02
IGL01480:Vmn2r58	APN	7	41,514,116 (GRCm39)	missense	probably benign	0.01
IGL01551:Vmn2r58	APN	7	41,514,703 (GRCm39)	missense	probably damaging	0.97
IGL01591:Vmn2r58	APN	7	41,514,753 (GRCm39)	missense	probably benign	0.03
IGL01940:Vmn2r58	APN	7	41,487,071 (GRCm39)	missense	probably benign	0.00
IGL01994:Vmn2r58	APN	7	41,486,394 (GRCm39)	missense	probably damaging	0.98
IGL02041:Vmn2r58	APN	7	41,514,703 (GRCm39)	missense	probably damaging	0.97
IGL02222:Vmn2r58	APN	7	41,513,449 (GRCm39)	missense	possibly damaging	0.81
IGL02317:Vmn2r58	APN	7	41,486,765 (GRCm39)	missense	possibly damaging	0.89
IGL02614:Vmn2r58	APN	7	41,486,553 (GRCm39)	missense	probably damaging	1.00
IGL02673:Vmn2r58	APN	7	41,514,082 (GRCm39)	missense	possibly damaging	0.90
IGL03323:Vmn2r58	APN	7	41,511,295 (GRCm39)	missense	probably benign	0.06
IGL03337:Vmn2r58	APN	7	41,513,810 (GRCm39)	missense	possibly damaging	0.93
IGL03380:Vmn2r58	APN	7	41,513,874 (GRCm39)	missense	probably benign	0.00
ANU05:Vmn2r58	UTSW	7	41,513,935 (GRCm39)	missense	probably benign	0.02
R0138:Vmn2r58	UTSW	7	41,487,048 (GRCm39)	missense	probably damaging	1.00
R0141:Vmn2r58	UTSW	7	41,511,309 (GRCm39)	missense	probably benign	0.11
R0421:Vmn2r58	UTSW	7	41,514,628 (GRCm39)	missense	probably benign	0.02
R0604:Vmn2r58	UTSW	7	41,510,000 (GRCm39)	missense	possibly damaging	0.78
R0854:Vmn2r58	UTSW	7	41,486,562 (GRCm39)	missense	probably damaging	1.00
R1413:Vmn2r58	UTSW	7	41,513,387 (GRCm39)	missense	probably benign	0.01
R1441:Vmn2r58	UTSW	7	41,486,864 (GRCm39)	missense	probably damaging	1.00
R1678:Vmn2r58	UTSW	7	41,513,480 (GRCm39)	missense	probably benign	0.40
R1691:Vmn2r58	UTSW	7	41,486,913 (GRCm39)	missense	possibly damaging	0.95
R1699:Vmn2r58	UTSW	7	41,509,951 (GRCm39)	missense	probably benign
R1865:Vmn2r58	UTSW	7	41,486,682 (GRCm39)	missense	possibly damaging	0.95
R2008:Vmn2r58	UTSW	7	41,509,924 (GRCm39)	missense	probably damaging	1.00
R2036:Vmn2r58	UTSW	7	41,513,417 (GRCm39)	missense	probably benign
R2202:Vmn2r58	UTSW	7	41,513,594 (GRCm39)	missense	probably benign	0.07
R3787:Vmn2r58	UTSW	7	41,513,498 (GRCm39)	missense	probably benign	0.01
R3883:Vmn2r58	UTSW	7	41,513,914 (GRCm39)	nonsense	probably null
R3944:Vmn2r58	UTSW	7	41,513,885 (GRCm39)	missense	probably benign	0.03
R3949:Vmn2r58	UTSW	7	41,513,348 (GRCm39)	missense	probably benign	0.08
R4232:Vmn2r58	UTSW	7	41,487,011 (GRCm39)	missense	possibly damaging	0.91
R4409:Vmn2r58	UTSW	7	41,522,051 (GRCm39)	missense	possibly damaging	0.69
R4411:Vmn2r58	UTSW	7	41,511,360 (GRCm39)	missense	possibly damaging	0.85
R4413:Vmn2r58	UTSW	7	41,511,360 (GRCm39)	missense	possibly damaging	0.85
R4600:Vmn2r58	UTSW	7	41,522,046 (GRCm39)	missense	probably benign	0.03
R4610:Vmn2r58	UTSW	7	41,487,117 (GRCm39)	missense	probably benign
R4646:Vmn2r58	UTSW	7	41,509,935 (GRCm39)	missense	probably damaging	0.96
R4793:Vmn2r58	UTSW	7	41,514,495 (GRCm39)	missense	probably damaging	0.99
R4870:Vmn2r58	UTSW	7	41,486,639 (GRCm39)	missense	possibly damaging	0.76
R4981:Vmn2r58	UTSW	7	41,486,885 (GRCm39)	missense	probably damaging	1.00
R4993:Vmn2r58	UTSW	7	41,487,176 (GRCm39)	missense	probably benign	0.00
R5024:Vmn2r58	UTSW	7	41,513,746 (GRCm39)	missense	probably damaging	0.99
R5064:Vmn2r58	UTSW	7	41,486,534 (GRCm39)	missense	probably damaging	0.99
R5330:Vmn2r58	UTSW	7	41,513,384 (GRCm39)	nonsense	probably null
R5526:Vmn2r58	UTSW	7	41,522,069 (GRCm39)	missense	probably benign	0.01
R5980:Vmn2r58	UTSW	7	41,514,480 (GRCm39)	missense	possibly damaging	0.81
R6163:Vmn2r58	UTSW	7	41,486,825 (GRCm39)	missense	probably benign	0.31
R6365:Vmn2r58	UTSW	7	41,513,607 (GRCm39)	missense	probably benign	0.42
R6567:Vmn2r58	UTSW	7	41,514,673 (GRCm39)	missense	probably benign	0.34
R6594:Vmn2r58	UTSW	7	41,486,535 (GRCm39)	missense	possibly damaging	0.69
R6980:Vmn2r58	UTSW	7	41,513,662 (GRCm39)	missense	possibly damaging	0.64
R7373:Vmn2r58	UTSW	7	41,487,212 (GRCm39)	missense	probably damaging	1.00
R7458:Vmn2r58	UTSW	7	41,487,123 (GRCm39)	missense	probably benign	0.06
R7630:Vmn2r58	UTSW	7	41,513,611 (GRCm39)	missense	probably damaging	0.99
R7807:Vmn2r58	UTSW	7	41,521,910 (GRCm39)	missense	probably benign	0.05
R8114:Vmn2r58	UTSW	7	41,511,392 (GRCm39)	missense	probably damaging	1.00
R8232:Vmn2r58	UTSW	7	41,514,076 (GRCm39)	missense	probably damaging	0.97
R8313:Vmn2r58	UTSW	7	41,521,952 (GRCm39)	missense	probably benign	0.01
R8412:Vmn2r58	UTSW	7	41,513,722 (GRCm39)	missense	probably benign	0.01
R8851:Vmn2r58	UTSW	7	41,487,219 (GRCm39)	missense	probably benign
R8881:Vmn2r58	UTSW	7	41,486,609 (GRCm39)	missense	probably benign	0.05
R8936:Vmn2r58	UTSW	7	41,513,981 (GRCm39)	missense
R9045:Vmn2r58	UTSW	7	41,487,087 (GRCm39)	missense	probably benign	0.00
R9166:Vmn2r58	UTSW	7	41,513,431 (GRCm39)	missense	probably damaging	1.00
R9706:Vmn2r58	UTSW	7	41,510,000 (GRCm39)	missense	probably damaging	0.99
RF006:Vmn2r58	UTSW	7	41,486,383 (GRCm39)	frame shift	probably null
RF027:Vmn2r58	UTSW	7	41,486,383 (GRCm39)	frame shift	probably null
Z1176:Vmn2r58	UTSW	7	41,513,789 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CTTCACCTTTGGCATATGGTTG -3'
(R):5'- GGAAAGTCTGGGTCTTGAGC -3'

Sequencing Primer
(F):5'- AGATTCATCTCATGGATACTATAGGC -3'
(R):5'- AGCTCTTCATGGGATGTTGATACCC -3'

Posted On

2021-01-18