Mutagenetix > Incidental Mutation

Incidental Mutation 'R8540:Cdk15'

659352

Institutional Source

Beutler Lab

Gene Symbol

Cdk15

Ensembl Gene

ENSMUSG00000026023

Gene Name

cyclin dependent kinase 15

Synonyms

Pftk2, Als2cr7

MMRRC Submission

068506-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8540 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

59296029-59391656 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 59349992 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 328 (V328A)

Ref Sequence

ENSEMBL: ENSMUSP00000124680 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000114248] [ENSMUST00000160662]

AlphaFold

Q3V3A1

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114248
AA Change: V326A

PolyPhen 2 Score 0.933 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000109886
Gene: ENSMUSG00000026023
AA Change: V326A

Domain	Start	End	E-Value	Type
S_TKc	101	385	7.9e-86	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000160662
AA Change: V328A

PolyPhen 2 Score 0.933 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000124680
Gene: ENSMUSG00000026023
AA Change: V328A

Domain	Start	End	E-Value	Type
S_TKc	103	387	7.9e-86	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (37/37)

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aebp2	A	T	6: 140,579,437 (GRCm39)	I225L	probably benign	Het
Aebp2	A	G	6: 140,579,439 (GRCm39)	I225M	probably benign	Het
Ap2a1	C	A	7: 44,553,750 (GRCm39)	G566C	probably damaging	Het
Cyld	G	A	8: 89,473,568 (GRCm39)	G929D	probably damaging	Het
Ddx50	G	A	10: 62,476,569 (GRCm39)	L235F	possibly damaging	Het
Dlg5	T	C	14: 24,208,767 (GRCm39)	D813G	probably damaging	Het
Epb41l3	C	T	17: 69,593,757 (GRCm39)	T797M	probably damaging	Het
Foxred2	G	A	15: 77,836,212 (GRCm39)	R382W	probably damaging	Het
Gm5798	T	A	14: 41,070,674 (GRCm39)	I28N	possibly damaging	Het
Grb14	C	T	2: 64,851,478 (GRCm39)	V91M	probably benign	Het
Ints6	T	C	14: 62,934,353 (GRCm39)	D718G	probably benign	Het
Man2a1	C	A	17: 64,965,982 (GRCm39)	H307N	probably benign	Het
Mylk	T	C	16: 34,750,257 (GRCm39)	Y1199H	possibly damaging	Het
Nim1k	T	C	13: 120,175,718 (GRCm39)	S163G	probably benign	Het
Or1r1	A	G	11: 73,875,153 (GRCm39)	F94L	probably damaging	Het
Or51f5	G	C	7: 102,424,339 (GRCm39)	A203P	possibly damaging	Het
Or5ac16	A	T	16: 59,022,323 (GRCm39)	H155Q	possibly damaging	Het
Or8h6	C	A	2: 86,703,776 (GRCm39)	C97F	probably damaging	Het
Phyhip	A	G	14: 70,704,594 (GRCm39)	D271G	probably benign	Het
Pramel27	T	A	4: 143,579,496 (GRCm39)	S360R	probably benign	Het
Pycr2	G	A	1: 180,734,178 (GRCm39)	A187T	possibly damaging	Het
Ryr3	T	A	2: 112,630,367 (GRCm39)	E2148V	probably damaging	Het
Septin12	T	C	16: 4,805,481 (GRCm39)	H304R	probably damaging	Het
Sorbs1	G	C	19: 40,365,244 (GRCm39)	R180G	probably benign	Het
Ssx2ip	T	C	3: 146,124,114 (GRCm39)	V43A	probably benign	Het
Syne2	A	T	12: 76,141,148 (GRCm39)	N6087Y	probably damaging	Het
Tbc1d4	A	T	14: 101,845,712 (GRCm39)	I62N	probably damaging	Het
Ttn	C	T	2: 76,573,337 (GRCm39)	G24106D	probably damaging	Het
Uhrf1	T	A	17: 56,612,105 (GRCm39)	I3N	probably damaging	Het
Urgcp	A	C	11: 5,667,915 (GRCm39)	L141R	probably damaging	Het
Ush2a	G	A	1: 188,274,858 (GRCm39)	R1777H	probably benign	Het
Vamp3	A	G	4: 151,135,507 (GRCm39)	V37A	possibly damaging	Het
Vmn1r172	C	T	7: 23,359,498 (GRCm39)	L128F	possibly damaging	Het
Vmn1r76	A	G	7: 11,664,897 (GRCm39)	Y71H	probably damaging	Het
Vmn2r111	C	T	17: 22,778,023 (GRCm39)		probably null	Het
Vmn2r111	T	A	17: 22,778,024 (GRCm39)	N552Y	probably damaging	Het
Vmn2r81	A	T	10: 79,129,065 (GRCm39)	N652I	probably damaging	Het
Vmn2r87	T	C	10: 130,314,762 (GRCm39)	N275D	possibly damaging	Het
Wrn	T	A	8: 33,842,154 (GRCm39)	I47F	probably damaging	Het

Other mutations in Cdk15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01307:Cdk15	APN	1	59,326,955 (GRCm39)	missense	probably benign	0.37
IGL01612:Cdk15	APN	1	59,328,932 (GRCm39)	missense	possibly damaging	0.93
IGL02179:Cdk15	APN	1	59,370,100 (GRCm39)	missense	possibly damaging	0.84
IGL03228:Cdk15	APN	1	59,297,912 (GRCm39)	missense	possibly damaging	0.95
FR4449:Cdk15	UTSW	1	59,296,982 (GRCm39)	small insertion	probably benign
R0270:Cdk15	UTSW	1	59,349,965 (GRCm39)	missense	probably damaging	0.99
R1720:Cdk15	UTSW	1	59,328,917 (GRCm39)	missense	probably damaging	1.00
R1958:Cdk15	UTSW	1	59,383,475 (GRCm39)	missense	probably damaging	0.99
R1969:Cdk15	UTSW	1	59,370,110 (GRCm39)	missense	probably damaging	1.00
R3159:Cdk15	UTSW	1	59,340,440 (GRCm39)	missense	probably damaging	1.00
R6000:Cdk15	UTSW	1	59,328,818 (GRCm39)	missense	probably damaging	1.00
R6226:Cdk15	UTSW	1	59,304,792 (GRCm39)	missense	probably damaging	1.00
R6257:Cdk15	UTSW	1	59,296,264 (GRCm39)	critical splice donor site	probably null
R7184:Cdk15	UTSW	1	59,304,814 (GRCm39)	missense	probably benign	0.33
R7446:Cdk15	UTSW	1	59,328,854 (GRCm39)	missense	probably damaging	1.00
R7467:Cdk15	UTSW	1	59,328,938 (GRCm39)	missense	probably null	0.96
R7588:Cdk15	UTSW	1	59,383,458 (GRCm39)	missense	possibly damaging	0.95
R9024:Cdk15	UTSW	1	59,326,957 (GRCm39)	missense	probably damaging	1.00
R9372:Cdk15	UTSW	1	59,370,142 (GRCm39)	missense	probably benign	0.00
R9404:Cdk15	UTSW	1	59,328,914 (GRCm39)	missense	possibly damaging	0.55
R9493:Cdk15	UTSW	1	59,326,943 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCTGTATGTCAGCTGGATCC -3'
(R):5'- TGGCATTGGAGAACTGATTTAACC -3'

Sequencing Primer
(F):5'- GGATCCCTGTGGCATCAGTAAATTC -3'
(R):5'- TGGTGGTACAAGCCTGAAATCCC -3'

Posted On

2021-01-18