Mutagenetix > Incidental Mutation

Incidental Mutation 'R8540:Vmn2r87'

659372

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r87

Ensembl Gene

ENSMUSG00000091511

Gene Name

vomeronasal 2, receptor 87

Synonyms

EG625131

MMRRC Submission

068506-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R8540 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

130307690-130333248 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 130314762 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Aspartic acid at position 275 (N275D)

Ref Sequence

ENSEMBL: ENSMUSP00000129215 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164227]

AlphaFold

E9PZX4

Predicted Effect

possibly damaging
Transcript: ENSMUST00000164227
AA Change: N275D

PolyPhen 2 Score 0.736 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000129215
Gene: ENSMUSG00000091511
AA Change: N275D

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	422	1.8e-27	PFAM
Pfam:NCD3G	508	562	1.8e-19	PFAM
Pfam:7tm_3	595	829	8.8e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (37/37)

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aebp2	A	T	6: 140,579,437 (GRCm39)	I225L	probably benign	Het
Aebp2	A	G	6: 140,579,439 (GRCm39)	I225M	probably benign	Het
Ap2a1	C	A	7: 44,553,750 (GRCm39)	G566C	probably damaging	Het
Cdk15	T	C	1: 59,349,992 (GRCm39)	V328A	possibly damaging	Het
Cyld	G	A	8: 89,473,568 (GRCm39)	G929D	probably damaging	Het
Ddx50	G	A	10: 62,476,569 (GRCm39)	L235F	possibly damaging	Het
Dlg5	T	C	14: 24,208,767 (GRCm39)	D813G	probably damaging	Het
Epb41l3	C	T	17: 69,593,757 (GRCm39)	T797M	probably damaging	Het
Foxred2	G	A	15: 77,836,212 (GRCm39)	R382W	probably damaging	Het
Gm5798	T	A	14: 41,070,674 (GRCm39)	I28N	possibly damaging	Het
Grb14	C	T	2: 64,851,478 (GRCm39)	V91M	probably benign	Het
Ints6	T	C	14: 62,934,353 (GRCm39)	D718G	probably benign	Het
Man2a1	C	A	17: 64,965,982 (GRCm39)	H307N	probably benign	Het
Mylk	T	C	16: 34,750,257 (GRCm39)	Y1199H	possibly damaging	Het
Nim1k	T	C	13: 120,175,718 (GRCm39)	S163G	probably benign	Het
Or1r1	A	G	11: 73,875,153 (GRCm39)	F94L	probably damaging	Het
Or51f5	G	C	7: 102,424,339 (GRCm39)	A203P	possibly damaging	Het
Or5ac16	A	T	16: 59,022,323 (GRCm39)	H155Q	possibly damaging	Het
Or8h6	C	A	2: 86,703,776 (GRCm39)	C97F	probably damaging	Het
Phyhip	A	G	14: 70,704,594 (GRCm39)	D271G	probably benign	Het
Pramel27	T	A	4: 143,579,496 (GRCm39)	S360R	probably benign	Het
Pycr2	G	A	1: 180,734,178 (GRCm39)	A187T	possibly damaging	Het
Ryr3	T	A	2: 112,630,367 (GRCm39)	E2148V	probably damaging	Het
Septin12	T	C	16: 4,805,481 (GRCm39)	H304R	probably damaging	Het
Sorbs1	G	C	19: 40,365,244 (GRCm39)	R180G	probably benign	Het
Ssx2ip	T	C	3: 146,124,114 (GRCm39)	V43A	probably benign	Het
Syne2	A	T	12: 76,141,148 (GRCm39)	N6087Y	probably damaging	Het
Tbc1d4	A	T	14: 101,845,712 (GRCm39)	I62N	probably damaging	Het
Ttn	C	T	2: 76,573,337 (GRCm39)	G24106D	probably damaging	Het
Uhrf1	T	A	17: 56,612,105 (GRCm39)	I3N	probably damaging	Het
Urgcp	A	C	11: 5,667,915 (GRCm39)	L141R	probably damaging	Het
Ush2a	G	A	1: 188,274,858 (GRCm39)	R1777H	probably benign	Het
Vamp3	A	G	4: 151,135,507 (GRCm39)	V37A	possibly damaging	Het
Vmn1r172	C	T	7: 23,359,498 (GRCm39)	L128F	possibly damaging	Het
Vmn1r76	A	G	7: 11,664,897 (GRCm39)	Y71H	probably damaging	Het
Vmn2r111	C	T	17: 22,778,023 (GRCm39)		probably null	Het
Vmn2r111	T	A	17: 22,778,024 (GRCm39)	N552Y	probably damaging	Het
Vmn2r81	A	T	10: 79,129,065 (GRCm39)	N652I	probably damaging	Het
Wrn	T	A	8: 33,842,154 (GRCm39)	I47F	probably damaging	Het

Other mutations in Vmn2r87

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01090:Vmn2r87	APN	10	130,333,247 (GRCm39)	start codon destroyed	probably null	1.00
IGL01295:Vmn2r87	APN	10	130,307,878 (GRCm39)	missense	probably damaging	1.00
IGL01411:Vmn2r87	APN	10	130,308,429 (GRCm39)	missense	probably benign	0.03
IGL01680:Vmn2r87	APN	10	130,315,586 (GRCm39)	nonsense	probably null
IGL01822:Vmn2r87	APN	10	130,307,991 (GRCm39)	missense	probably damaging	1.00
IGL01835:Vmn2r87	APN	10	130,314,978 (GRCm39)	missense	probably damaging	1.00
IGL01965:Vmn2r87	APN	10	130,314,924 (GRCm39)	missense	possibly damaging	0.49
IGL02562:Vmn2r87	APN	10	130,314,513 (GRCm39)	missense	probably damaging	1.00
IGL02665:Vmn2r87	APN	10	130,333,049 (GRCm39)	missense	probably benign	0.16
IGL03202:Vmn2r87	APN	10	130,333,091 (GRCm39)	missense	probably benign
FR4304:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4340:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4342:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
FR4589:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
LCD18:Vmn2r87	UTSW	10	130,314,583 (GRCm39)	missense	probably benign	0.01
R0344:Vmn2r87	UTSW	10	130,315,806 (GRCm39)	missense	probably damaging	1.00
R0374:Vmn2r87	UTSW	10	130,307,848 (GRCm39)	missense	probably damaging	1.00
R0384:Vmn2r87	UTSW	10	130,307,712 (GRCm39)	missense	probably benign
R1144:Vmn2r87	UTSW	10	130,312,098 (GRCm39)	splice site	probably benign
R1172:Vmn2r87	UTSW	10	130,313,453 (GRCm39)	missense	probably benign	0.03
R1860:Vmn2r87	UTSW	10	130,315,755 (GRCm39)	missense	probably benign	0.00
R1866:Vmn2r87	UTSW	10	130,308,441 (GRCm39)	missense	possibly damaging	0.88
R1897:Vmn2r87	UTSW	10	130,307,829 (GRCm39)	missense	probably damaging	1.00
R2360:Vmn2r87	UTSW	10	130,315,631 (GRCm39)	missense	probably damaging	0.99
R2909:Vmn2r87	UTSW	10	130,314,865 (GRCm39)	missense	probably damaging	0.99
R3874:Vmn2r87	UTSW	10	130,315,856 (GRCm39)	missense	possibly damaging	0.62
R4113:Vmn2r87	UTSW	10	130,315,691 (GRCm39)	missense	probably benign
R4190:Vmn2r87	UTSW	10	130,308,556 (GRCm39)	missense	probably damaging	1.00
R4197:Vmn2r87	UTSW	10	130,315,779 (GRCm39)	missense	possibly damaging	0.55
R4201:Vmn2r87	UTSW	10	130,308,448 (GRCm39)	missense	probably benign	0.03
R4202:Vmn2r87	UTSW	10	130,308,448 (GRCm39)	missense	probably benign	0.03
R4368:Vmn2r87	UTSW	10	130,315,676 (GRCm39)	missense	probably benign	0.44
R4485:Vmn2r87	UTSW	10	130,315,678 (GRCm39)	nonsense	probably null
R4537:Vmn2r87	UTSW	10	130,308,054 (GRCm39)	missense	probably benign	0.12
R4590:Vmn2r87	UTSW	10	130,315,014 (GRCm39)	missense	possibly damaging	0.69
R4752:Vmn2r87	UTSW	10	130,314,336 (GRCm39)	nonsense	probably null
R4873:Vmn2r87	UTSW	10	130,308,367 (GRCm39)	missense	probably damaging	1.00
R4875:Vmn2r87	UTSW	10	130,308,367 (GRCm39)	missense	probably damaging	1.00
R4923:Vmn2r87	UTSW	10	130,314,435 (GRCm39)	missense	probably damaging	0.99
R4970:Vmn2r87	UTSW	10	130,314,422 (GRCm39)	missense	probably damaging	1.00
R5049:Vmn2r87	UTSW	10	130,308,298 (GRCm39)	missense	probably damaging	0.96
R5112:Vmn2r87	UTSW	10	130,314,422 (GRCm39)	missense	probably damaging	1.00
R5187:Vmn2r87	UTSW	10	130,333,208 (GRCm39)	missense	probably null	0.99
R5618:Vmn2r87	UTSW	10	130,315,817 (GRCm39)	missense	probably damaging	1.00
R6057:Vmn2r87	UTSW	10	130,308,226 (GRCm39)	missense	probably benign	0.02
R6220:Vmn2r87	UTSW	10	130,315,807 (GRCm39)	missense	probably benign	0.01
R6287:Vmn2r87	UTSW	10	130,314,291 (GRCm39)	critical splice donor site	probably null
R6383:Vmn2r87	UTSW	10	130,314,869 (GRCm39)	missense	probably damaging	1.00
R6576:Vmn2r87	UTSW	10	130,314,654 (GRCm39)	missense	probably benign	0.05
R6742:Vmn2r87	UTSW	10	130,308,396 (GRCm39)	missense	probably damaging	1.00
R7086:Vmn2r87	UTSW	10	130,333,178 (GRCm39)	missense	probably benign	0.00
R7162:Vmn2r87	UTSW	10	130,313,416 (GRCm39)	missense	probably benign	0.08
R7419:Vmn2r87	UTSW	10	130,307,992 (GRCm39)	missense	probably damaging	1.00
R7425:Vmn2r87	UTSW	10	130,314,761 (GRCm39)	missense	probably damaging	1.00
R7443:Vmn2r87	UTSW	10	130,308,588 (GRCm39)	missense	probably damaging	1.00
R7571:Vmn2r87	UTSW	10	130,314,940 (GRCm39)	missense	probably damaging	0.99
R7663:Vmn2r87	UTSW	10	130,308,054 (GRCm39)	missense	probably damaging	0.97
R7716:Vmn2r87	UTSW	10	130,308,018 (GRCm39)	missense	probably benign	0.09
R7793:Vmn2r87	UTSW	10	130,313,413 (GRCm39)	missense	probably benign	0.05
R7806:Vmn2r87	UTSW	10	130,315,679 (GRCm39)	missense	probably benign
R7841:Vmn2r87	UTSW	10	130,333,095 (GRCm39)	missense	probably benign	0.31
R8326:Vmn2r87	UTSW	10	130,308,180 (GRCm39)	missense	possibly damaging	0.67
R8411:Vmn2r87	UTSW	10	130,308,126 (GRCm39)	missense	probably damaging	0.99
R8445:Vmn2r87	UTSW	10	130,313,335 (GRCm39)	missense	probably damaging	1.00
R8892:Vmn2r87	UTSW	10	130,308,105 (GRCm39)	missense	probably damaging	0.99
R9093:Vmn2r87	UTSW	10	130,308,165 (GRCm39)	missense	probably benign	0.08
R9623:Vmn2r87	UTSW	10	130,315,794 (GRCm39)	missense	probably damaging	1.00
R9667:Vmn2r87	UTSW	10	130,314,776 (GRCm39)	missense	probably damaging	1.00
R9797:Vmn2r87	UTSW	10	130,312,064 (GRCm39)	missense	probably benign	0.44
R9797:Vmn2r87	UTSW	10	130,308,138 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r87	UTSW	10	130,308,183 (GRCm39)	missense	probably damaging	0.98
Z1176:Vmn2r87	UTSW	10	130,307,713 (GRCm39)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- CAGTGCTGTCCATTCCAATG -3'
(R):5'- ACCAGGGTATTCAGCTTCATTC -3'

Sequencing Primer
(F):5'- ACTGACTGAGAAATGTTTATTGGG -3'
(R):5'- TCATTCAGATTTAAGAGAAGAAAGCC -3'

Posted On

2021-01-18