Incidental Mutation 'R8557:Limk2'
ID |
660168 |
Institutional Source |
Beutler Lab
|
Gene Symbol |
Limk2
|
Ensembl Gene |
ENSMUSG00000020451 |
Gene Name |
LIM domain kinase 2 |
Synonyms |
whe, Limk2b, Limk2a, A930024P04Rik, LIM kinase 2 |
MMRRC Submission |
068520-MU
|
Accession Numbers |
|
Essential gene? |
Probably non essential
(E-score: 0.186)
|
Stock # |
R8557 (G1)
|
Quality Score |
225.009 |
Status
|
Validated
|
Chromosome |
11 |
Chromosomal Location |
3294256-3359189 bp(-) (GRCm39) |
Type of Mutation |
missense |
DNA Base Change (assembly) |
A to G
at 3296379 bp (GRCm39)
|
Zygosity |
Heterozygous |
Amino Acid Change |
Valine to Alanine
at position 356
(V356A)
|
Ref Sequence |
ENSEMBL: ENSMUSP00000105656
(fasta)
|
Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000045153]
[ENSMUST00000101638]
[ENSMUST00000101640]
[ENSMUST00000101642]
[ENSMUST00000110029]
[ENSMUST00000136474]
[ENSMUST00000136536]
|
AlphaFold |
O54785 |
Predicted Effect |
probably benign
Transcript: ENSMUST00000045153
|
SMART Domains |
Protein: ENSMUSP00000036921 Gene: ENSMUSG00000034614
Domain | Start | End | E-Value | Type |
signal peptide
|
1 |
21 |
N/A |
INTRINSIC |
KR
|
23 |
103 |
3.64e-17 |
SMART |
transmembrane domain
|
170 |
192 |
N/A |
INTRINSIC |
|
Predicted Effect |
possibly damaging
Transcript: ENSMUST00000101638
AA Change: V543A
PolyPhen 2
Score 0.895 (Sensitivity: 0.82; Specificity: 0.94)
|
SMART Domains |
Protein: ENSMUSP00000099162 Gene: ENSMUSG00000020451 AA Change: V543A
Domain | Start | End | E-Value | Type |
LIM
|
11 |
63 |
2e-14 |
SMART |
LIM
|
71 |
124 |
4.63e-10 |
SMART |
PDZ
|
161 |
239 |
7.04e-10 |
SMART |
low complexity region
|
241 |
255 |
N/A |
INTRINSIC |
low complexity region
|
280 |
306 |
N/A |
INTRINSIC |
low complexity region
|
310 |
322 |
N/A |
INTRINSIC |
Pfam:Pkinase
|
331 |
600 |
5.3e-48 |
PFAM |
Pfam:Pkinase_Tyr
|
331 |
601 |
4.7e-50 |
PFAM |
Pfam:Kdo
|
341 |
497 |
8.6e-7 |
PFAM |
|
Predicted Effect |
possibly damaging
Transcript: ENSMUST00000101640
AA Change: V528A
PolyPhen 2
Score 0.867 (Sensitivity: 0.83; Specificity: 0.93)
|
SMART Domains |
Protein: ENSMUSP00000099163 Gene: ENSMUSG00000020451 AA Change: V528A
Domain | Start | End | E-Value | Type |
LIM
|
7 |
42 |
4.91e-1 |
SMART |
LIM
|
50 |
103 |
4.63e-10 |
SMART |
PDZ
|
140 |
218 |
7.04e-10 |
SMART |
low complexity region
|
220 |
234 |
N/A |
INTRINSIC |
low complexity region
|
259 |
285 |
N/A |
INTRINSIC |
low complexity region
|
289 |
301 |
N/A |
INTRINSIC |
Pfam:Pkinase
|
310 |
582 |
1.2e-45 |
PFAM |
Pfam:Pkinase_Tyr
|
310 |
586 |
1.3e-51 |
PFAM |
|
Predicted Effect |
possibly damaging
Transcript: ENSMUST00000101642
AA Change: V522A
PolyPhen 2
Score 0.839 (Sensitivity: 0.84; Specificity: 0.93)
|
SMART Domains |
Protein: ENSMUSP00000099165 Gene: ENSMUSG00000020451 AA Change: V522A
Domain | Start | End | E-Value | Type |
LIM
|
7 |
42 |
4.91e-1 |
SMART |
LIM
|
50 |
103 |
4.63e-10 |
SMART |
PDZ
|
140 |
218 |
7.04e-10 |
SMART |
low complexity region
|
220 |
234 |
N/A |
INTRINSIC |
low complexity region
|
259 |
285 |
N/A |
INTRINSIC |
low complexity region
|
289 |
301 |
N/A |
INTRINSIC |
Pfam:Pkinase
|
310 |
579 |
4.9e-48 |
PFAM |
Pfam:Pkinase_Tyr
|
310 |
580 |
4.3e-50 |
PFAM |
Pfam:Kdo
|
320 |
476 |
8.2e-7 |
PFAM |
|
Predicted Effect |
possibly damaging
Transcript: ENSMUST00000110029
AA Change: V356A
PolyPhen 2
Score 0.895 (Sensitivity: 0.82; Specificity: 0.94)
|
SMART Domains |
Protein: ENSMUSP00000105656 Gene: ENSMUSG00000020451 AA Change: V356A
Domain | Start | End | E-Value | Type |
PDZ
|
1 |
52 |
4.55e-1 |
SMART |
low complexity region
|
54 |
68 |
N/A |
INTRINSIC |
low complexity region
|
93 |
119 |
N/A |
INTRINSIC |
low complexity region
|
123 |
135 |
N/A |
INTRINSIC |
Pfam:Pkinase
|
144 |
411 |
2.7e-49 |
PFAM |
Pfam:Pkinase_Tyr
|
144 |
414 |
1.7e-51 |
PFAM |
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000136474
|
SMART Domains |
Protein: ENSMUSP00000119106 Gene: ENSMUSG00000034614
Domain | Start | End | E-Value | Type |
transmembrane domain
|
69 |
91 |
N/A |
INTRINSIC |
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000136536
|
SMART Domains |
Protein: ENSMUSP00000122407 Gene: ENSMUSG00000034614
Domain | Start | End | E-Value | Type |
signal peptide
|
1 |
21 |
N/A |
INTRINSIC |
KR
|
23 |
103 |
1.1e-16 |
SMART |
transmembrane domain
|
177 |
199 |
N/A |
INTRINSIC |
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000138907
|
SMART Domains |
Protein: ENSMUSP00000121277 Gene: ENSMUSG00000034614
Domain | Start | End | E-Value | Type |
KR
|
1 |
41 |
7.93e-3 |
SMART |
transmembrane domain
|
108 |
130 |
N/A |
INTRINSIC |
|
Coding Region Coverage |
- 1x: 100.0%
- 3x: 100.0%
- 10x: 99.8%
- 20x: 99.3%
|
Validation Efficiency |
100% (60/60) |
MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] PHENOTYPE: Male homozygotes for targeted null mutations exhibit small testes but are fertile. Mutant kidneys have fewer glomeruli and dilated renal tubules, but function normally. Mice homozygous for a gene trap allele or spontaneous mutation have open eyelids at birth, corneal abnormalities and inflammation. [provided by MGI curators]
|
Allele List at MGI |
|
Other mutations in this stock |
Total: 61 list
Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
0610040J01Rik |
T |
C |
5: 64,055,954 (GRCm39) |
V230A |
probably benign |
Het |
Ablim2 |
A |
T |
5: 35,985,483 (GRCm39) |
H260L |
probably damaging |
Het |
Acp4 |
C |
T |
7: 43,905,272 (GRCm39) |
|
probably null |
Het |
Acvr2b |
G |
T |
9: 119,261,654 (GRCm39) |
V416L |
probably damaging |
Het |
Adipoq |
A |
T |
16: 22,965,430 (GRCm39) |
|
probably benign |
Het |
Amhr2 |
A |
G |
15: 102,362,847 (GRCm39) |
Q538R |
probably benign |
Het |
Apoh |
C |
T |
11: 108,300,062 (GRCm39) |
T237I |
probably damaging |
Het |
Aspm |
C |
T |
1: 139,384,494 (GRCm39) |
T97I |
probably benign |
Het |
Atg16l2 |
A |
T |
7: 100,939,863 (GRCm39) |
H499Q |
probably benign |
Het |
Bicdl2 |
G |
A |
17: 23,886,536 (GRCm39) |
R375H |
probably benign |
Het |
C3 |
T |
C |
17: 57,531,383 (GRCm39) |
T304A |
probably benign |
Het |
Cast |
A |
T |
13: 74,852,301 (GRCm39) |
D647E |
probably damaging |
Het |
Chrm2 |
T |
C |
6: 36,501,010 (GRCm39) |
V289A |
probably benign |
Het |
Clock |
T |
C |
5: 76,377,217 (GRCm39) |
D694G |
probably damaging |
Het |
Cyp4a31 |
C |
T |
4: 115,427,438 (GRCm39) |
R215C |
possibly damaging |
Het |
Dctn2 |
T |
C |
10: 127,114,062 (GRCm39) |
L334P |
probably damaging |
Het |
Dnah11 |
A |
G |
12: 117,842,247 (GRCm39) |
I4444T |
probably benign |
Het |
Egf |
C |
A |
3: 129,548,600 (GRCm39) |
R5L |
unknown |
Het |
Ercc1 |
A |
G |
7: 19,082,480 (GRCm39) |
N11S |
probably benign |
Het |
Eri3 |
T |
C |
4: 117,472,520 (GRCm39) |
S113P |
possibly damaging |
Het |
Fasn |
A |
G |
11: 120,706,610 (GRCm39) |
V958A |
probably benign |
Het |
Fgf10 |
A |
G |
13: 118,918,132 (GRCm39) |
K140R |
probably benign |
Het |
Gfra2 |
G |
A |
14: 71,214,737 (GRCm39) |
M163I |
probably benign |
Het |
Gm14403 |
T |
C |
2: 177,201,354 (GRCm39) |
I191T |
probably damaging |
Het |
Hdlbp |
T |
A |
1: 93,341,219 (GRCm39) |
E977D |
probably damaging |
Het |
Hectd4 |
A |
G |
5: 121,448,714 (GRCm39) |
N354S |
possibly damaging |
Het |
Hpgds |
A |
T |
6: 65,096,999 (GRCm39) |
I170N |
probably benign |
Het |
Map4 |
A |
G |
9: 109,893,370 (GRCm39) |
|
probably null |
Het |
Mettl13 |
C |
T |
1: 162,371,921 (GRCm39) |
G316D |
possibly damaging |
Het |
Mia2 |
T |
A |
12: 59,148,274 (GRCm39) |
L45I |
probably damaging |
Het |
Mier1 |
T |
A |
4: 102,996,543 (GRCm39) |
|
probably null |
Het |
Mob3a |
G |
T |
10: 80,527,008 (GRCm39) |
R106S |
probably benign |
Het |
Mug2 |
T |
A |
6: 122,040,660 (GRCm39) |
F737I |
probably damaging |
Het |
Myo7a |
C |
T |
7: 97,703,081 (GRCm39) |
A2053T |
probably benign |
Het |
Myrip |
A |
G |
9: 120,246,252 (GRCm39) |
S127G |
probably benign |
Het |
Naaladl2 |
T |
A |
3: 24,262,528 (GRCm39) |
K395I |
probably benign |
Het |
Nedd4l |
A |
T |
18: 65,336,986 (GRCm39) |
M678L |
probably benign |
Het |
Nefh |
A |
T |
11: 4,891,233 (GRCm39) |
I462N |
probably damaging |
Het |
Nfe2 |
A |
G |
15: 103,157,025 (GRCm39) |
M322T |
probably benign |
Het |
Ogfr |
GGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCCAAAGCCAGGTGG |
GGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCCAAAGCCAGGTGG |
2: 180,237,059 (GRCm39) |
|
probably benign |
Het |
Or2z2 |
T |
A |
11: 58,346,562 (GRCm39) |
D71V |
probably damaging |
Het |
Or56b1 |
T |
A |
7: 104,285,103 (GRCm39) |
V74E |
possibly damaging |
Het |
Pkdcc |
A |
G |
17: 83,528,495 (GRCm39) |
N288S |
probably benign |
Het |
Sacs |
G |
A |
14: 61,444,725 (GRCm39) |
C2257Y |
probably damaging |
Het |
Sec23a |
G |
T |
12: 59,052,056 (GRCm39) |
Q90K |
probably damaging |
Het |
Shisal2a |
T |
A |
4: 108,225,085 (GRCm39) |
N159I |
probably benign |
Het |
Slc30a6 |
A |
G |
17: 74,712,685 (GRCm39) |
T61A |
possibly damaging |
Het |
Slc39a11 |
C |
A |
11: 113,141,385 (GRCm39) |
A234S |
probably damaging |
Het |
Smg6 |
C |
A |
11: 75,047,064 (GRCm39) |
N1252K |
probably damaging |
Het |
Spata2 |
A |
T |
2: 167,326,227 (GRCm39) |
D197E |
probably damaging |
Het |
Spen |
C |
T |
4: 141,197,681 (GRCm39) |
A3396T |
probably benign |
Het |
Spocd1 |
T |
C |
4: 129,842,761 (GRCm39) |
F59L |
probably damaging |
Het |
Tectb |
T |
A |
19: 55,181,105 (GRCm39) |
|
probably benign |
Het |
Tnnc1 |
G |
A |
14: 30,932,562 (GRCm39) |
V82I |
probably damaging |
Het |
Traf7 |
A |
T |
17: 24,729,015 (GRCm39) |
V513D |
probably damaging |
Het |
Tut4 |
T |
A |
4: 108,399,908 (GRCm39) |
|
probably null |
Het |
Vmn2r111 |
A |
T |
17: 22,790,910 (GRCm39) |
Y129* |
probably null |
Het |
Vrtn |
T |
A |
12: 84,696,690 (GRCm39) |
F480Y |
probably benign |
Het |
Vwa8 |
A |
T |
14: 79,246,649 (GRCm39) |
I664F |
probably damaging |
Het |
Vwde |
A |
T |
6: 13,193,136 (GRCm39) |
V401E |
probably damaging |
Het |
Zfp655 |
A |
T |
5: 145,180,835 (GRCm39) |
N231I |
probably benign |
Het |
|
Other mutations in Limk2 |
Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
IGL01105:Limk2
|
APN |
11 |
3,305,475 (GRCm39) |
splice site |
probably benign |
|
IGL01592:Limk2
|
APN |
11 |
3,309,052 (GRCm39) |
missense |
probably benign |
0.00 |
IGL01716:Limk2
|
APN |
11 |
3,308,990 (GRCm39) |
splice site |
probably null |
|
IGL01911:Limk2
|
APN |
11 |
3,305,340 (GRCm39) |
missense |
probably benign |
|
R0900:Limk2
|
UTSW |
11 |
3,300,731 (GRCm39) |
missense |
probably damaging |
1.00 |
R1587:Limk2
|
UTSW |
11 |
3,303,455 (GRCm39) |
missense |
possibly damaging |
0.82 |
R1632:Limk2
|
UTSW |
11 |
3,296,250 (GRCm39) |
missense |
probably damaging |
1.00 |
R1695:Limk2
|
UTSW |
11 |
3,303,275 (GRCm39) |
critical splice donor site |
probably null |
|
R1712:Limk2
|
UTSW |
11 |
3,308,104 (GRCm39) |
splice site |
probably null |
|
R1792:Limk2
|
UTSW |
11 |
3,308,236 (GRCm39) |
missense |
probably benign |
|
R1982:Limk2
|
UTSW |
11 |
3,305,461 (GRCm39) |
missense |
probably benign |
0.00 |
R3009:Limk2
|
UTSW |
11 |
3,309,046 (GRCm39) |
missense |
probably benign |
0.01 |
R4565:Limk2
|
UTSW |
11 |
3,298,634 (GRCm39) |
missense |
probably damaging |
0.98 |
R4703:Limk2
|
UTSW |
11 |
3,297,586 (GRCm39) |
nonsense |
probably null |
|
R4978:Limk2
|
UTSW |
11 |
3,359,069 (GRCm39) |
utr 5 prime |
probably benign |
|
R5160:Limk2
|
UTSW |
11 |
3,300,772 (GRCm39) |
missense |
probably damaging |
1.00 |
R5460:Limk2
|
UTSW |
11 |
3,302,332 (GRCm39) |
missense |
probably benign |
0.30 |
R6497:Limk2
|
UTSW |
11 |
3,310,492 (GRCm39) |
missense |
probably benign |
0.00 |
R6543:Limk2
|
UTSW |
11 |
3,300,682 (GRCm39) |
missense |
probably damaging |
1.00 |
R6666:Limk2
|
UTSW |
11 |
3,310,493 (GRCm39) |
missense |
probably damaging |
1.00 |
R7054:Limk2
|
UTSW |
11 |
3,305,448 (GRCm39) |
missense |
possibly damaging |
0.95 |
R7330:Limk2
|
UTSW |
11 |
3,296,311 (GRCm39) |
missense |
probably benign |
0.39 |
R7681:Limk2
|
UTSW |
11 |
3,303,354 (GRCm39) |
missense |
probably damaging |
0.96 |
R7722:Limk2
|
UTSW |
11 |
3,306,092 (GRCm39) |
splice site |
probably null |
|
R7745:Limk2
|
UTSW |
11 |
3,305,896 (GRCm39) |
missense |
probably damaging |
0.99 |
R8120:Limk2
|
UTSW |
11 |
3,298,589 (GRCm39) |
splice site |
probably null |
|
R8193:Limk2
|
UTSW |
11 |
3,297,691 (GRCm39) |
missense |
possibly damaging |
0.79 |
R8379:Limk2
|
UTSW |
11 |
3,321,162 (GRCm39) |
start gained |
probably benign |
|
R8708:Limk2
|
UTSW |
11 |
3,300,763 (GRCm39) |
missense |
probably benign |
0.19 |
R9617:Limk2
|
UTSW |
11 |
3,297,715 (GRCm39) |
missense |
probably damaging |
0.99 |
|
Predicted Primers |
PCR Primer
(F):5'- GTTGATCTAAAGCAAGCGTCTCG -3'
(R):5'- TCCTCAGTATCCGGACTCTAGAG -3'
Sequencing Primer
(F):5'- TCGGGCCAGAGTGCTGTAAG -3'
(R):5'- TATCCGGACTCTAGAGAGGGGTC -3'
|
Posted On |
2021-01-18 |