Mutagenetix > Incidental Mutation

Incidental Mutation 'R8671:Tnc'

661147

Institutional Source

Beutler Lab

Gene Symbol

Tnc

Ensembl Gene

ENSMUSG00000028364

Gene Name

tenascin C

Synonyms

TN, TN-C, hexabrachion, tenascin-C, C130033P17Rik, cytotactin, Hxb

MMRRC Submission

068526-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8671 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

63959785-64047015 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 64017446 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 418 (C418S)

Ref Sequence

ENSEMBL: ENSMUSP00000030056 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030056] [ENSMUST00000107371] [ENSMUST00000107372] [ENSMUST00000107377]

AlphaFold

Q80YX1

Predicted Effect

probably damaging
Transcript: ENSMUST00000030056
AA Change: C418S

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000030056
Gene: ENSMUSG00000028364
AA Change: C418S

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000107371
AA Change: C418S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000102994
Gene: ENSMUSG00000028364
AA Change: C418S

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
Pfam:hEGF	173	185	4e-4	PFAM
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000107372
AA Change: C418S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000102995
Gene: ENSMUSG00000028364
AA Change: C418S

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	2.75e0	SMART
FN3	1529	1608	3.4e-4	SMART
FN3	1619	1697	1.55e-7	SMART
FN3	1708	1785	1.53e-6	SMART
FN3	1796	1873	7.75e-8	SMART
FBG	1888	2098	4.08e-124	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000107377
AA Change: C418S

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000103000
Gene: ENSMUSG00000028364
AA Change: C418S

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

100% (48/48)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]
PHENOTYPE: Mice homozygous for several different targeted mutations show variable behavioral and nervous system phenotypes such as abnormal circadian rhythm, anxiety behavior, novelty-induced activity, swimming, impaired synaptic plasticity, long term potentiation and serotonin and dopamine neurotransmission. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310002L09Rik	C	T	4: 73,942,929	E145K	probably damaging	Het
4930430A15Rik	T	C	2: 111,229,532		probably benign	Het
4930467E23Rik	T	A	8: 19,734,759	L203*	probably null	Het
Ambp	A	T	4: 63,150,419	Y120*	probably null	Het
Ankrd36	C	T	11: 5,629,312	A192V	probably benign	Het
Apol7e	A	T	15: 77,717,603	M134L	probably benign	Het
Atg9b	A	T	5: 24,386,109	N774K	probably benign	Het
BC027072	G	T	17: 71,751,377	A435E	probably benign	Het
Catsperb	T	A	12: 101,594,337	N862K	possibly damaging	Het
Ccdc7a	T	A	8: 128,920,467	D648V	probably damaging	Het
Cfap54	G	T	10: 92,955,072	P1855T	unknown	Het
Clcnkb	T	A	4: 141,412,230	T154S	probably damaging	Het
Cux1	C	T	5: 136,250,600	R609H	probably damaging	Het
Eif2ak4	A	G	2: 118,422,186	D413G	possibly damaging	Het
Flnc	T	C	6: 29,443,502		probably null	Het
Grm5	T	C	7: 88,116,290		probably null	Het
Hectd3	T	G	4: 116,996,581	F225V	possibly damaging	Het
Hpcal4	G	T	4: 123,189,183	M107I	probably benign	Het
Idh2	TCCCAGG	T	7: 80,098,331		probably benign	Het
Ikbkap	A	G	4: 56,771,453	L948P	probably damaging	Het
Larp4	C	A	15: 100,010,458	Q607K	probably benign	Het
March8	A	G	6: 116,401,854	R250G	probably benign	Het
Med13	A	C	11: 86,271,097	N2135K	probably damaging	Het
Msh5	T	A	17: 35,045,933	R89*	probably null	Het
Musk	T	G	4: 58,286,051		probably benign	Het
Myo18b	T	C	5: 112,874,743	Q261R	unknown	Het
Npr1	A	T	3: 90,456,157		probably benign	Het
Nynrin	T	A	14: 55,870,442	V1002E	possibly damaging	Het
Olfr1138	A	T	2: 87,737,646	L226Q	probably damaging	Het
Olfr1195	A	T	2: 88,683,105	F209Y	probably benign	Het
Olfr1315-ps1	T	A	2: 112,110,988	K88M	probably damaging	Het
Olfr167	T	C	16: 19,515,054	Y194C	possibly damaging	Het
Pcdh9	T	C	14: 93,888,650	Y28C	probably damaging	Het
Pmpca	G	C	2: 26,395,034	E424Q	possibly damaging	Het
Prkd1	T	A	12: 50,388,408	N512I	probably benign	Het
Pwwp2b	C	T	7: 139,256,410	P589L	probably damaging	Het
Rasgrp4	G	A	7: 29,143,027	G242D	probably damaging	Het
Rcbtb1	T	G	14: 59,230,524	V480G	probably damaging	Het
Rptn	A	G	3: 93,398,194	S945G	probably benign	Het
Slc22a2	C	T	17: 12,605,976	Q242*	probably null	Het
Slfn3	T	A	11: 83,212,999	V232E	probably benign	Het
Stab1	T	A	14: 31,157,408	K705M	probably damaging	Het
Syn2	C	A	6: 115,278,167	S480*	probably null	Het
Thsd4	A	T	9: 60,394,445	L189H	probably damaging	Het
Tinagl1	T	C	4: 130,167,804	T250A	probably benign	Het
Ube2q1	A	G	3: 89,776,078	E110G	probably damaging	Het
Wdfy4	G	T	14: 32,971,765		probably benign	Het
Xcl1	T	C	1: 164,931,850	M94V	probably benign	Het
Zfp369	T	C	13: 65,296,281	S413P	possibly damaging	Het
Zfp454	A	C	11: 50,873,768	I279S	possibly damaging	Het
Zfp971	A	C	2: 178,033,937	Q443P	probably damaging	Het

Other mutations in Tnc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00229:Tnc	APN	4	64,016,824 (GRCm38)	splice site	probably benign
IGL00531:Tnc	APN	4	63,971,153 (GRCm38)	splice site	probably benign
IGL00674:Tnc	APN	4	63,965,607 (GRCm38)	missense	probably damaging	1.00
IGL01015:Tnc	APN	4	64,017,334 (GRCm38)	missense	probably benign	0.19
IGL01090:Tnc	APN	4	64,000,080 (GRCm38)	missense	probably damaging	1.00
IGL01310:Tnc	APN	4	64,013,077 (GRCm38)	missense	probably benign	0.03
IGL01331:Tnc	APN	4	63,982,875 (GRCm38)	missense	probably damaging	0.99
IGL01393:Tnc	APN	4	64,014,054 (GRCm38)	splice site	probably benign
IGL01411:Tnc	APN	4	64,000,722 (GRCm38)	missense	probably damaging	0.96
IGL01472:Tnc	APN	4	64,006,419 (GRCm38)	missense	probably benign	0.00
IGL01552:Tnc	APN	4	63,970,408 (GRCm38)	missense	probably damaging	1.00
IGL01661:Tnc	APN	4	63,970,307 (GRCm38)	splice site	probably benign
IGL01669:Tnc	APN	4	64,000,701 (GRCm38)	missense	probably damaging	1.00
IGL01912:Tnc	APN	4	64,008,740 (GRCm38)	missense	probably damaging	1.00
IGL02028:Tnc	APN	4	63,966,672 (GRCm38)	splice site	probably benign
IGL02100:Tnc	APN	4	64,000,161 (GRCm38)	missense	possibly damaging	0.84
IGL02549:Tnc	APN	4	64,015,072 (GRCm38)	missense	probably damaging	1.00
IGL02642:Tnc	APN	4	63,965,579 (GRCm38)	splice site	probably benign
IGL02712:Tnc	APN	4	63,975,256 (GRCm38)	missense	probably damaging	1.00
IGL02876:Tnc	APN	4	64,015,101 (GRCm38)	missense	possibly damaging	0.56
IGL02886:Tnc	APN	4	64,000,107 (GRCm38)	missense	probably damaging	0.96
IGL02972:Tnc	APN	4	63,976,478 (GRCm38)	missense	probably benign	0.11
IGL03073:Tnc	APN	4	63,971,224 (GRCm38)	missense	possibly damaging	0.58
IGL03116:Tnc	APN	4	64,014,033 (GRCm38)	missense	probably damaging	1.00
IGL03181:Tnc	APN	4	63,967,306 (GRCm38)	missense	possibly damaging	0.95
IGL03358:Tnc	APN	4	64,017,615 (GRCm38)	nonsense	probably null
tancredo	UTSW	4	63,993,297 (GRCm38)	nonsense	probably null
BB009:Tnc	UTSW	4	64,008,620 (GRCm38)	missense	probably benign
BB019:Tnc	UTSW	4	64,008,620 (GRCm38)	missense	probably benign
P0020:Tnc	UTSW	4	64,008,857 (GRCm38)	missense	possibly damaging	0.63
PIT4377001:Tnc	UTSW	4	64,017,736 (GRCm38)	missense	probably damaging	1.00
PIT4403001:Tnc	UTSW	4	63,964,667 (GRCm38)	missense	probably damaging	1.00
PIT4468001:Tnc	UTSW	4	63,964,667 (GRCm38)	missense	probably damaging	1.00
R0243:Tnc	UTSW	4	63,970,420 (GRCm38)	missense	probably damaging	0.98
R0362:Tnc	UTSW	4	64,017,442 (GRCm38)	missense	probably damaging	1.00
R0410:Tnc	UTSW	4	64,007,694 (GRCm38)	missense	probably benign	0.00
R0420:Tnc	UTSW	4	64,000,159 (GRCm38)	missense	probably benign	0.00
R0540:Tnc	UTSW	4	64,020,455 (GRCm38)	missense	probably damaging	1.00
R0650:Tnc	UTSW	4	64,008,734 (GRCm38)	missense	probably benign	0.00
R1019:Tnc	UTSW	4	63,962,082 (GRCm38)	missense	probably damaging	1.00
R1102:Tnc	UTSW	4	64,020,468 (GRCm38)	missense	probably benign	0.05
R1126:Tnc	UTSW	4	64,018,120 (GRCm38)	missense	probably damaging	0.99
R1141:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1142:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1307:Tnc	UTSW	4	64,008,859 (GRCm38)	missense	probably damaging	0.98
R1322:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1414:Tnc	UTSW	4	63,965,695 (GRCm38)	splice site	probably benign
R1470:Tnc	UTSW	4	63,966,574 (GRCm38)	missense	probably damaging	1.00
R1470:Tnc	UTSW	4	63,966,574 (GRCm38)	missense	probably damaging	1.00
R1499:Tnc	UTSW	4	63,964,754 (GRCm38)	missense	probably benign	0.15
R1506:Tnc	UTSW	4	64,007,684 (GRCm38)	missense	possibly damaging	0.90
R1597:Tnc	UTSW	4	64,006,384 (GRCm38)	missense	probably benign
R1750:Tnc	UTSW	4	63,972,735 (GRCm38)	missense	probably damaging	1.00
R1765:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1783:Tnc	UTSW	4	64,018,096 (GRCm38)	missense	probably damaging	0.98
R1808:Tnc	UTSW	4	63,999,931 (GRCm38)	missense	probably damaging	1.00
R1903:Tnc	UTSW	4	64,000,062 (GRCm38)	missense	probably benign	0.00
R1932:Tnc	UTSW	4	63,993,025 (GRCm38)	critical splice donor site	probably null
R1941:Tnc	UTSW	4	64,014,964 (GRCm38)	missense	probably damaging	1.00
R1983:Tnc	UTSW	4	63,984,630 (GRCm38)	missense	possibly damaging	0.95
R2024:Tnc	UTSW	4	63,964,621 (GRCm38)	missense	probably damaging	1.00
R2075:Tnc	UTSW	4	63,995,666 (GRCm38)	missense	possibly damaging	0.94
R2327:Tnc	UTSW	4	63,975,238 (GRCm38)	missense	possibly damaging	0.78
R2444:Tnc	UTSW	4	64,014,963 (GRCm38)	missense	probably damaging	1.00
R2982:Tnc	UTSW	4	64,020,519 (GRCm38)	missense	possibly damaging	0.81
R3874:Tnc	UTSW	4	64,008,710 (GRCm38)	missense	probably damaging	1.00
R4110:Tnc	UTSW	4	64,014,951 (GRCm38)	missense	probably damaging	1.00
R4360:Tnc	UTSW	4	64,016,924 (GRCm38)	missense	probably benign	0.35
R4371:Tnc	UTSW	4	63,970,351 (GRCm38)	missense	probably damaging	1.00
R4434:Tnc	UTSW	4	64,007,829 (GRCm38)	missense	possibly damaging	0.91
R4438:Tnc	UTSW	4	64,007,829 (GRCm38)	missense	possibly damaging	0.91
R4570:Tnc	UTSW	4	63,995,672 (GRCm38)	missense	probably damaging	0.99
R4595:Tnc	UTSW	4	63,995,745 (GRCm38)	missense	probably damaging	1.00
R4749:Tnc	UTSW	4	63,995,639 (GRCm38)	missense	possibly damaging	0.56
R4756:Tnc	UTSW	4	63,967,343 (GRCm38)	missense	probably damaging	0.99
R4824:Tnc	UTSW	4	64,017,620 (GRCm38)	nonsense	probably null
R4957:Tnc	UTSW	4	63,976,556 (GRCm38)	missense	probably damaging	1.00
R4977:Tnc	UTSW	4	64,006,248 (GRCm38)	missense	possibly damaging	0.82
R5001:Tnc	UTSW	4	64,000,062 (GRCm38)	missense	probably benign	0.16
R5001:Tnc	UTSW	4	63,984,489 (GRCm38)	missense	probably damaging	1.00
R5015:Tnc	UTSW	4	64,006,502 (GRCm38)	missense	probably damaging	1.00
R5049:Tnc	UTSW	4	64,017,986 (GRCm38)	missense	probably damaging	1.00
R5066:Tnc	UTSW	4	63,975,229 (GRCm38)	missense	probably damaging	0.96
R5073:Tnc	UTSW	4	64,020,411 (GRCm38)	missense	probably damaging	1.00
R5116:Tnc	UTSW	4	63,967,215 (GRCm38)	critical splice donor site	probably null
R5195:Tnc	UTSW	4	63,967,252 (GRCm38)	missense	probably damaging	1.00
R5200:Tnc	UTSW	4	63,971,278 (GRCm38)	missense	probably damaging	1.00
R5221:Tnc	UTSW	4	63,993,297 (GRCm38)	nonsense	probably null
R5237:Tnc	UTSW	4	63,962,096 (GRCm38)	missense	probably damaging	1.00
R5265:Tnc	UTSW	4	63,993,206 (GRCm38)	missense	probably benign	0.00
R5275:Tnc	UTSW	4	63,964,730 (GRCm38)	nonsense	probably null
R5346:Tnc	UTSW	4	64,008,655 (GRCm38)	missense	probably benign
R5409:Tnc	UTSW	4	64,007,417 (GRCm38)	missense	probably damaging	1.00
R5409:Tnc	UTSW	4	63,966,536 (GRCm38)	missense	probably damaging	1.00
R5469:Tnc	UTSW	4	64,013,925 (GRCm38)	splice site	probably null
R5518:Tnc	UTSW	4	64,017,679 (GRCm38)	missense	probably damaging	1.00
R5560:Tnc	UTSW	4	64,008,709 (GRCm38)	missense	probably damaging	1.00
R5588:Tnc	UTSW	4	64,006,422 (GRCm38)	missense	possibly damaging	0.57
R5686:Tnc	UTSW	4	64,007,730 (GRCm38)	splice site	probably null
R5686:Tnc	UTSW	4	64,008,795 (GRCm38)	missense	possibly damaging	0.78
R5837:Tnc	UTSW	4	64,013,214 (GRCm38)	missense	probably damaging	1.00
R5976:Tnc	UTSW	4	64,018,166 (GRCm38)	missense	probably benign	0.17
R6156:Tnc	UTSW	4	63,970,352 (GRCm38)	missense	probably damaging	1.00
R6182:Tnc	UTSW	4	64,008,796 (GRCm38)	missense	probably damaging	0.99
R6360:Tnc	UTSW	4	64,000,733 (GRCm38)	missense	probably damaging	1.00
R6416:Tnc	UTSW	4	64,007,816 (GRCm38)	missense	probably benign	0.05
R6778:Tnc	UTSW	4	63,995,598 (GRCm38)	missense	probably benign	0.12
R6798:Tnc	UTSW	4	63,965,604 (GRCm38)	missense	probably benign	0.02
R6799:Tnc	UTSW	4	63,965,604 (GRCm38)	missense	probably benign	0.02
R6943:Tnc	UTSW	4	63,982,745 (GRCm38)	missense	probably damaging	0.97
R7027:Tnc	UTSW	4	63,984,589 (GRCm38)	missense	probably benign	0.02
R7183:Tnc	UTSW	4	64,013,128 (GRCm38)	missense	probably damaging	1.00
R7204:Tnc	UTSW	4	63,971,155 (GRCm38)	splice site	probably null
R7317:Tnc	UTSW	4	63,972,722 (GRCm38)	missense	probably damaging	0.99
R7323:Tnc	UTSW	4	63,971,232 (GRCm38)	missense	probably damaging	0.96
R7327:Tnc	UTSW	4	63,964,762 (GRCm38)	splice site	probably null
R7382:Tnc	UTSW	4	64,014,043 (GRCm38)	nonsense	probably null
R7399:Tnc	UTSW	4	64,020,657 (GRCm38)	start gained	probably benign
R7479:Tnc	UTSW	4	64,017,628 (GRCm38)	missense	possibly damaging	0.95
R7585:Tnc	UTSW	4	64,020,411 (GRCm38)	missense	probably damaging	1.00
R7932:Tnc	UTSW	4	64,008,620 (GRCm38)	missense	probably benign
R7947:Tnc	UTSW	4	64,017,343 (GRCm38)	missense	probably damaging	1.00
R7974:Tnc	UTSW	4	64,000,724 (GRCm38)	missense	possibly damaging	0.84
R7991:Tnc	UTSW	4	64,008,746 (GRCm38)	missense	probably benign	0.42
R8004:Tnc	UTSW	4	63,984,657 (GRCm38)	missense	probably benign	0.04
R8080:Tnc	UTSW	4	63,976,469 (GRCm38)	missense	possibly damaging	0.52
R8109:Tnc	UTSW	4	64,008,763 (GRCm38)	missense	probably benign	0.11
R8145:Tnc	UTSW	4	64,017,479 (GRCm38)	missense	probably benign
R8340:Tnc	UTSW	4	64,007,799 (GRCm38)	missense	probably damaging	1.00
R8360:Tnc	UTSW	4	63,967,274 (GRCm38)	missense	probably benign	0.00
R8691:Tnc	UTSW	4	63,962,076 (GRCm38)	missense	probably damaging	1.00
R8759:Tnc	UTSW	4	64,006,264 (GRCm38)	missense	possibly damaging	0.86
R8864:Tnc	UTSW	4	63,993,059 (GRCm38)	missense	probably damaging	0.98
R8927:Tnc	UTSW	4	64,007,358 (GRCm38)	missense	probably damaging	1.00
R8928:Tnc	UTSW	4	64,007,358 (GRCm38)	missense	probably damaging	1.00
R8949:Tnc	UTSW	4	64,008,850 (GRCm38)	missense	probably damaging	1.00
R8956:Tnc	UTSW	4	64,000,733 (GRCm38)	missense	probably damaging	1.00
R9016:Tnc	UTSW	4	64,017,094 (GRCm38)	missense	probably benign	0.23
R9049:Tnc	UTSW	4	64,000,010 (GRCm38)	missense	possibly damaging	0.83
R9097:Tnc	UTSW	4	63,970,385 (GRCm38)	missense	possibly damaging	0.62
R9114:Tnc	UTSW	4	63,972,736 (GRCm38)	missense	probably benign	0.03
R9151:Tnc	UTSW	4	64,020,449 (GRCm38)	missense	possibly damaging	0.46
R9488:Tnc	UTSW	4	63,995,705 (GRCm38)	missense	probably damaging	0.99
R9537:Tnc	UTSW	4	63,966,584 (GRCm38)	missense	probably damaging	0.99
R9666:Tnc	UTSW	4	64,007,808 (GRCm38)	missense	probably damaging	1.00
R9700:Tnc	UTSW	4	64,014,949 (GRCm38)	missense	probably damaging	0.99
R9703:Tnc	UTSW	4	63,971,175 (GRCm38)	missense	probably benign	0.00
R9771:Tnc	UTSW	4	64,007,363 (GRCm38)	missense	probably damaging	1.00
S24628:Tnc	UTSW	4	64,018,012 (GRCm38)	missense	probably damaging	1.00
Z1177:Tnc	UTSW	4	64,007,426 (GRCm38)	nonsense	probably null
Z1177:Tnc	UTSW	4	63,960,544 (GRCm38)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- CCAGTGTAGTCGTCATCACAG -3'
(R):5'- AGTGTGTTTGCAACGAGGGC -3'

Sequencing Primer
(F):5'- ATTCACACAGCGGCCATGTTG -3'
(R):5'- TGCAACGAGGGCTTTGC -3'

Posted On

2021-03-08