Mutagenetix > Incidental Mutation

Incidental Mutation 'R8676:Herc2'

661386

Institutional Source

Beutler Lab

Gene Symbol

Herc2

Ensembl Gene

ENSMUSG00000030451

Gene Name

HECT and RLD domain containing E3 ubiquitin protein ligase 2

Synonyms

D7H15F37S1, D7H15F32S1, rjs, jdf2, D15F32S1h

MMRRC Submission

068531-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.921) question?

Stock #

R8676 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

56050196-56231800 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 56188613 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Lysine at position 3296 (T3296K)

Ref Sequence

ENSEMBL: ENSMUSP00000075579 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076226] [ENSMUST00000164095] [ENSMUST00000205303]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000076226
AA Change: T3296K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000075579
Gene: ENSMUSG00000030451
AA Change: T3296K

Domain	Start	End	E-Value	Type
low complexity region	73	87	N/A	INTRINSIC
low complexity region	164	175	N/A	INTRINSIC
low complexity region	198	212	N/A	INTRINSIC
low complexity region	272	281	N/A	INTRINSIC
low complexity region	304	317	N/A	INTRINSIC
Pfam:RCC1	514	567	7.6e-16	PFAM
Pfam:RCC1_2	554	583	6e-9	PFAM
Pfam:RCC1	570	615	7.1e-17	PFAM
Pfam:RCC1_2	606	637	6.9e-8	PFAM
Pfam:RCC1	624	673	7.2e-15	PFAM
Pfam:RCC1	676	725	3.3e-18	PFAM
Pfam:RCC1_2	712	740	1.6e-9	PFAM
low complexity region	854	866	N/A	INTRINSIC
low complexity region	902	913	N/A	INTRINSIC
coiled coil region	950	977	N/A	INTRINSIC
low complexity region	1052	1066	N/A	INTRINSIC
Cyt-b5	1211	1284	1.08e-1	SMART
low complexity region	1310	1316	N/A	INTRINSIC
low complexity region	1440	1446	N/A	INTRINSIC
low complexity region	1545	1563	N/A	INTRINSIC
coiled coil region	1651	1674	N/A	INTRINSIC
Pfam:MIB_HERC2	1871	1933	5.8e-29	PFAM
low complexity region	1939	1952	N/A	INTRINSIC
low complexity region	2211	2222	N/A	INTRINSIC
low complexity region	2381	2388	N/A	INTRINSIC
low complexity region	2402	2416	N/A	INTRINSIC
low complexity region	2521	2540	N/A	INTRINSIC
Pfam:Cul7	2555	2633	2.6e-43	PFAM
ZnF_ZZ	2703	2747	5.39e-11	SMART
APC10	2780	2933	5.1e-41	SMART
TECPR	2978	3019	7.59e0	SMART
Pfam:RCC1_2	3048	3079	9.2e-8	PFAM
Pfam:RCC1	3066	3115	3.7e-17	PFAM
Pfam:RCC1_2	3102	3131	3.9e-11	PFAM
Pfam:RCC1	3118	3162	1.9e-15	PFAM
Pfam:RCC1	3172	3221	9.6e-15	PFAM
Pfam:RCC1_2	3208	3236	2.2e-7	PFAM
TECPR	3241	3284	1.32e2	SMART
low complexity region	3357	3365	N/A	INTRINSIC
low complexity region	3430	3447	N/A	INTRINSIC
low complexity region	3480	3495	N/A	INTRINSIC
low complexity region	3755	3771	N/A	INTRINSIC
TECPR	3972	4012	2.41e1	SMART
Pfam:RCC1_2	4041	4072	5.1e-8	PFAM
Pfam:RCC1	4059	4108	1.5e-16	PFAM
Pfam:RCC1	4111	4155	7.9e-16	PFAM
TECPR	4184	4225	8.42e1	SMART
Pfam:RCC1_2	4253	4282	4.3e-10	PFAM
Pfam:RCC1	4269	4318	1.2e-17	PFAM
Blast:HECTc	4340	4425	6e-18	BLAST
HECTc	4455	4801	1.37e-62	SMART
low complexity region	4808	4828	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000164095
AA Change: T3296K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000131573
Gene: ENSMUSG00000030451
AA Change: T3296K

Domain	Start	End	E-Value	Type
low complexity region	73	87	N/A	INTRINSIC
low complexity region	164	175	N/A	INTRINSIC
low complexity region	198	212	N/A	INTRINSIC
low complexity region	272	281	N/A	INTRINSIC
low complexity region	304	317	N/A	INTRINSIC
Pfam:RCC1	514	567	2.9e-15	PFAM
Pfam:RCC1_2	554	583	1.6e-8	PFAM
Pfam:RCC1	570	615	2.3e-16	PFAM
Pfam:RCC1	624	673	1.2e-14	PFAM
Pfam:RCC1_2	660	689	2.1e-7	PFAM
Pfam:RCC1	676	725	9.8e-18	PFAM
Pfam:RCC1_2	712	740	1.1e-9	PFAM
low complexity region	854	866	N/A	INTRINSIC
low complexity region	902	913	N/A	INTRINSIC
coiled coil region	950	977	N/A	INTRINSIC
low complexity region	1052	1066	N/A	INTRINSIC
Cyt-b5	1211	1284	1.08e-1	SMART
low complexity region	1310	1316	N/A	INTRINSIC
low complexity region	1440	1446	N/A	INTRINSIC
low complexity region	1545	1563	N/A	INTRINSIC
coiled coil region	1651	1674	N/A	INTRINSIC
Pfam:MIB_HERC2	1871	1931	9.3e-25	PFAM
low complexity region	1939	1952	N/A	INTRINSIC
low complexity region	2211	2222	N/A	INTRINSIC
low complexity region	2381	2388	N/A	INTRINSIC
low complexity region	2402	2416	N/A	INTRINSIC
low complexity region	2521	2540	N/A	INTRINSIC
Pfam:Cul7	2555	2632	1.4e-39	PFAM
ZnF_ZZ	2703	2747	5.39e-11	SMART
APC10	2780	2933	5.1e-41	SMART
TECPR	2978	3019	7.59e0	SMART
Pfam:RCC1_2	3048	3079	1.6e-7	PFAM
Pfam:RCC1	3066	3115	7.1e-16	PFAM
Pfam:RCC1_2	3102	3131	7.1e-11	PFAM
Pfam:RCC1	3118	3163	1.2e-14	PFAM
Pfam:RCC1	3172	3221	4.7e-15	PFAM
TECPR	3241	3284	1.32e2	SMART
low complexity region	3357	3365	N/A	INTRINSIC
low complexity region	3430	3447	N/A	INTRINSIC
low complexity region	3480	3495	N/A	INTRINSIC
low complexity region	3755	3771	N/A	INTRINSIC
TECPR	3972	4012	2.41e1	SMART
Pfam:RCC1_2	4041	4072	1.2e-7	PFAM
Pfam:RCC1	4059	4108	9.6e-15	PFAM
Pfam:RCC1	4111	4156	5.6e-15	PFAM
TECPR	4184	4225	8.42e1	SMART
Pfam:RCC1_2	4253	4282	7.3e-10	PFAM
Pfam:RCC1	4269	4318	1.6e-16	PFAM
Blast:HECTc	4340	4425	6e-18	BLAST
HECTc	4455	4801	1.37e-62	SMART
low complexity region	4808	4828	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000205303
AA Change: T3296K

PolyPhen 2 Score 0.080 (Sensitivity: 0.93; Specificity: 0.85)

Meta Mutation Damage Score

0.5621

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

93% (54/58)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene belongs to the HERC gene family that encodes a group of unusually large proteins, which contain multiple structural domains. All members have at least 1 copy of an N-terminal region showing homology to the cell cycle regulator RCC1 and a C-terminal HECT (homologous to E6-AP C terminus) domain found in a number of E3 ubiquitin protein ligases. Genetic variations in this gene are associated with skin/hair/eye pigmentation variability. Multiple pseudogenes of this gene are located on chromosomes 15 and 16. [provided by RefSeq, Mar 2012]
PHENOTYPE: Homozygotes for null mutations exhibit runting, nervousness, and incoordination. Males are sterile with sperm abnormalities, while females show reduced fertility and impaired maternal ability. Also see alleles at the Oca2 (p) locus for deletions that encompass the Herc2 gene. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610037L13Rik	A	G	4: 107,895,599 (GRCm38)	N152D	unknown	Het
1700021F05Rik	A	G	10: 43,532,937 (GRCm38)	L70S	probably benign	Het
Acsm3	T	A	7: 119,775,169 (GRCm38)	S281R	probably damaging	Het
Adipor2	G	T	6: 119,363,486 (GRCm38)		probably benign	Het
Alk	T	C	17: 71,897,941 (GRCm38)	S1079G	probably damaging	Het
Ankrd27	T	C	7: 35,602,584 (GRCm38)		probably null	Het
Anxa6	C	A	11: 55,001,282 (GRCm38)	E283*	probably null	Het
Bnc2	T	C	4: 84,276,313 (GRCm38)	H858R	possibly damaging	Het
Btnl6	T	C	17: 34,508,069 (GRCm38)	S496G	probably benign	Het
Ccdc88a	T	C	11: 29,460,860 (GRCm38)	S449P	probably benign	Het
Cdh23	A	T	10: 60,410,910 (GRCm38)	D916E	probably damaging	Het
Cfap43	A	T	19: 47,748,017 (GRCm38)	L1345H	possibly damaging	Het
Cyld	A	T	8: 88,729,510 (GRCm38)	H396L	probably benign	Het
Cyp20a1	A	G	1: 60,379,420 (GRCm38)	T340A	possibly damaging	Het
Dera	A	T	6: 137,830,204 (GRCm38)	I217F	probably damaging	Het
Dnah11	A	G	12: 118,190,804 (GRCm38)	L247P	probably damaging	Het
Eftud2	G	A	11: 102,868,621 (GRCm38)	T152M	probably damaging	Het
Epb41l2	C	T	10: 25,443,776 (GRCm38)	T169M	probably benign	Het
Fam186a	T	C	15: 99,947,142 (GRCm38)	D407G	unknown	Het
Fam189a2	T	C	19: 23,988,494 (GRCm38)	K214E	probably damaging	Het
Gli3	T	A	13: 15,715,034 (GRCm38)	C578S	probably damaging	Het
Gm436	G	A	4: 144,670,113 (GRCm38)	R350C	possibly damaging	Het
Gm6408	A	G	5: 146,482,427 (GRCm38)	N84S	probably benign	Het
Heatr5a	AGCACACTGCAGGAAGCTCACACAGCACAGCATACCTTCAGGAGTGCACACTGCAGGAAGCTCACACAGCACAGCATACCTTCAGGAGAGCACACTGCAGGAAGCTCA	AGCACACTGCAGGAAGCTCACACAGCACAGCATACCTTCAGGAGAGCACACTGCAGGAAGCTCA	12: 51,887,919 (GRCm38)		probably benign	Het
Hnf4g	A	T	3: 3,643,073 (GRCm38)		probably benign	Het
Hyal4	C	A	6: 24,755,827 (GRCm38)	Q15K	probably damaging	Het
Itpr3	G	A	17: 27,118,677 (GRCm38)		probably benign	Het
Kcna3	A	G	3: 107,036,592 (GRCm38)	E57G	probably damaging	Het
Kcnc3	C	A	7: 44,591,596 (GRCm38)	D237E	probably benign	Het
Map3k8	A	G	18: 4,343,137 (GRCm38)	V130A	probably benign	Het
Mpp7	A	G	18: 7,440,430 (GRCm38)		probably null	Het
Myh13	T	A	11: 67,342,485 (GRCm38)	L610Q	probably damaging	Het
Olfr1020	T	A	2: 85,849,902 (GRCm38)	M150K	probably benign	Het
Olfr1339	C	A	4: 118,735,038 (GRCm38)	P170T	probably damaging	Het
Olfr917	T	C	9: 38,665,768 (GRCm38)	I25M	probably benign	Het
Pcdhb5	A	T	18: 37,321,076 (GRCm38)	T170S	probably benign	Het
Polr3b	T	A	10: 84,680,387 (GRCm38)	H626Q	probably benign	Het
Prkg1	A	T	19: 31,764,746 (GRCm38)	L26Q	probably damaging	Het
Prob1	T	C	18: 35,653,986 (GRCm38)	N405S	possibly damaging	Het
Proz	T	G	8: 13,073,630 (GRCm38)	S300R	probably damaging	Het
Psg19	A	G	7: 18,794,065 (GRCm38)	I251T	probably benign	Het
Rcbtb1	T	C	14: 59,229,952 (GRCm38)	I413T	possibly damaging	Het
Rnf144b	T	A	13: 47,228,976 (GRCm38)	Y103N	probably damaging	Het
Rspry1	G	C	8: 94,632,119 (GRCm38)	G194R	probably benign	Het
Scn2b	A	G	9: 45,125,619 (GRCm38)	I142V	probably damaging	Het
Spata20	A	T	11: 94,481,781 (GRCm38)	L588H	probably damaging	Het
Stk32b	T	A	5: 37,457,159 (GRCm38)	H335L	probably benign	Het
Taar4	A	C	10: 23,960,903 (GRCm38)	D137A	possibly damaging	Het
Tchh	CTCCGCCGGGAGCAAGAGCTCCGCCGGGAGCAAGAGTTCCGCCGGGAGCAAGAGCTCCGCCGGGAGCAAGAGTTCCGCCGGGAGCAAGAGCTCCGCC	CTCCGCCGGGAGCAAGAGCTCCGCCGGGAGCAAGAGTTCCGCCGGGAGCAAGAGCTCCGCC	3: 93,446,708 (GRCm38)		probably benign	Het
Tek	A	T	4: 94,849,837 (GRCm38)	H708L	probably benign	Het
Tmem89	C	T	9: 108,915,027 (GRCm38)	L132F	unknown	Het
Ugt2b38	T	C	5: 87,411,822 (GRCm38)	I404V	probably benign	Het
Vmn1r16	C	T	6: 57,322,829 (GRCm38)	M269I	probably benign	Het
Vmn1r201	A	G	13: 22,475,252 (GRCm38)	K212R	probably damaging	Het
Vmn2r11	A	C	5: 109,053,760 (GRCm38)	F293V	probably damaging	Het
Zdhhc17	A	T	10: 110,962,379 (GRCm38)		probably benign	Het
Zfp423	C	A	8: 87,782,710 (GRCm38)	M335I	probably benign	Het
Zfp74	T	C	7: 29,934,654 (GRCm38)	Y543C	probably damaging	Het
Zfp975	A	T	7: 42,662,840 (GRCm38)	S116R	probably benign	Het

Other mutations in Herc2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00329:Herc2	APN	7	56,124,299 (GRCm38)	missense	probably damaging	1.00
IGL00529:Herc2	APN	7	56,157,753 (GRCm38)	missense	probably benign
IGL00548:Herc2	APN	7	56,206,565 (GRCm38)	missense	probably benign	0.20
IGL00970:Herc2	APN	7	56,181,064 (GRCm38)	splice site	probably benign
IGL01141:Herc2	APN	7	56,212,841 (GRCm38)	missense	possibly damaging	0.47
IGL01147:Herc2	APN	7	56,156,949 (GRCm38)	missense	probably benign	0.43
IGL01150:Herc2	APN	7	56,181,133 (GRCm38)	missense	probably damaging	1.00
IGL01519:Herc2	APN	7	56,103,950 (GRCm38)	missense	probably damaging	1.00
IGL01576:Herc2	APN	7	56,226,661 (GRCm38)	critical splice donor site	probably null
IGL01626:Herc2	APN	7	56,085,142 (GRCm38)	missense	probably benign	0.02
IGL01658:Herc2	APN	7	56,159,452 (GRCm38)	missense	probably damaging	1.00
IGL01707:Herc2	APN	7	56,165,187 (GRCm38)	missense	probably damaging	1.00
IGL01727:Herc2	APN	7	56,137,806 (GRCm38)	missense	probably damaging	1.00
IGL01935:Herc2	APN	7	56,153,793 (GRCm38)	missense	probably benign
IGL01969:Herc2	APN	7	56,185,831 (GRCm38)	splice site	probably benign
IGL02074:Herc2	APN	7	56,087,444 (GRCm38)	splice site	probably benign
IGL02261:Herc2	APN	7	56,206,744 (GRCm38)	missense	probably damaging	0.99
IGL02339:Herc2	APN	7	56,121,722 (GRCm38)	missense	probably benign	0.01
IGL02353:Herc2	APN	7	56,114,812 (GRCm38)	missense	probably damaging	1.00
IGL02360:Herc2	APN	7	56,114,812 (GRCm38)	missense	probably damaging	1.00
IGL02409:Herc2	APN	7	56,220,469 (GRCm38)	splice site	probably null
IGL02528:Herc2	APN	7	56,108,893 (GRCm38)	splice site	probably benign
IGL02571:Herc2	APN	7	56,153,386 (GRCm38)	missense	probably damaging	1.00
IGL02578:Herc2	APN	7	56,106,535 (GRCm38)	splice site	probably null
IGL02661:Herc2	APN	7	56,113,073 (GRCm38)	missense	probably damaging	1.00
IGL02664:Herc2	APN	7	56,135,678 (GRCm38)	nonsense	probably null
IGL02675:Herc2	APN	7	56,164,101 (GRCm38)	missense	probably damaging	0.99
IGL02689:Herc2	APN	7	56,165,283 (GRCm38)	splice site	probably benign
IGL02710:Herc2	APN	7	56,137,814 (GRCm38)	missense	possibly damaging	0.95
IGL02750:Herc2	APN	7	56,204,379 (GRCm38)	splice site	probably benign
IGL02754:Herc2	APN	7	56,097,498 (GRCm38)	missense	probably damaging	1.00
IGL03029:Herc2	APN	7	56,168,967 (GRCm38)	missense	probably damaging	1.00
IGL03039:Herc2	APN	7	56,169,021 (GRCm38)	splice site	probably benign
IGL03082:Herc2	APN	7	56,185,923 (GRCm38)	missense	probably benign	0.19
IGL03090:Herc2	APN	7	56,204,473 (GRCm38)	missense	probably damaging	0.96
IGL03154:Herc2	APN	7	56,202,159 (GRCm38)	missense	probably damaging	1.00
IGL03165:Herc2	APN	7	56,191,912 (GRCm38)	missense	probably damaging	1.00
IGL03201:Herc2	APN	7	56,219,768 (GRCm38)	missense	probably damaging	1.00
IGL03234:Herc2	APN	7	56,103,862 (GRCm38)	missense	probably damaging	1.00
IGL03293:Herc2	APN	7	56,155,130 (GRCm38)	missense	probably benign	0.43
IGL03331:Herc2	APN	7	56,135,267 (GRCm38)	splice site	probably benign
IGL03340:Herc2	APN	7	56,090,920 (GRCm38)	missense	possibly damaging	0.51
IGL03409:Herc2	APN	7	56,228,569 (GRCm38)	missense	probably damaging	1.00
alarmed	UTSW	7	56,229,662 (GRCm38)	missense	possibly damaging	0.92
hyper	UTSW	7	56,159,417 (GRCm38)	missense	probably damaging	1.00
R0798_herc2_487	UTSW	7	56,135,683 (GRCm38)	critical splice donor site	probably null
R1370_Herc2_948	UTSW	7	56,168,873 (GRCm38)	missense	probably benign	0.01
R2030_Herc2_144	UTSW	7	56,184,373 (GRCm38)	missense	probably damaging	0.99
uptight	UTSW	7	56,113,210 (GRCm38)	missense	probably damaging	1.00
I0000:Herc2	UTSW	7	56,136,729 (GRCm38)	splice site	probably benign
PIT1430001:Herc2	UTSW	7	56,226,954 (GRCm38)	missense	probably damaging	1.00
R0009:Herc2	UTSW	7	56,207,812 (GRCm38)	missense	probably benign	0.03
R0009:Herc2	UTSW	7	56,207,812 (GRCm38)	missense	probably benign	0.03
R0058:Herc2	UTSW	7	56,170,483 (GRCm38)	missense	possibly damaging	0.93
R0114:Herc2	UTSW	7	56,153,774 (GRCm38)	splice site	probably benign
R0117:Herc2	UTSW	7	56,213,611 (GRCm38)	splice site	probably benign
R0141:Herc2	UTSW	7	56,121,561 (GRCm38)	missense	probably benign	0.17
R0266:Herc2	UTSW	7	56,206,578 (GRCm38)	missense	probably damaging	1.00
R0401:Herc2	UTSW	7	56,157,732 (GRCm38)	missense	probably damaging	0.99
R0403:Herc2	UTSW	7	56,159,417 (GRCm38)	missense	probably damaging	1.00
R0437:Herc2	UTSW	7	56,219,815 (GRCm38)	nonsense	probably null
R0491:Herc2	UTSW	7	56,122,366 (GRCm38)	missense	possibly damaging	0.54
R0499:Herc2	UTSW	7	56,184,369 (GRCm38)	nonsense	probably null
R0580:Herc2	UTSW	7	56,138,791 (GRCm38)	missense	probably damaging	1.00
R0650:Herc2	UTSW	7	56,113,210 (GRCm38)	missense	probably damaging	1.00
R0744:Herc2	UTSW	7	56,206,036 (GRCm38)	splice site	probably benign
R0798:Herc2	UTSW	7	56,135,683 (GRCm38)	critical splice donor site	probably null
R0842:Herc2	UTSW	7	56,121,705 (GRCm38)	missense	probably benign
R0849:Herc2	UTSW	7	56,206,578 (GRCm38)	missense	probably damaging	1.00
R0850:Herc2	UTSW	7	56,204,483 (GRCm38)	missense	probably benign	0.09
R0926:Herc2	UTSW	7	56,132,548 (GRCm38)	missense	possibly damaging	0.67
R1146:Herc2	UTSW	7	56,146,696 (GRCm38)	missense	probably benign
R1146:Herc2	UTSW	7	56,146,696 (GRCm38)	missense	probably benign
R1292:Herc2	UTSW	7	56,197,203 (GRCm38)	missense	probably benign	0.05
R1370:Herc2	UTSW	7	56,168,873 (GRCm38)	missense	probably benign	0.01
R1443:Herc2	UTSW	7	56,204,733 (GRCm38)	missense	possibly damaging	0.69
R1445:Herc2	UTSW	7	56,168,996 (GRCm38)	missense	probably damaging	1.00
R1541:Herc2	UTSW	7	56,135,657 (GRCm38)	missense	probably damaging	1.00
R1550:Herc2	UTSW	7	56,135,658 (GRCm38)	missense	probably damaging	1.00
R1551:Herc2	UTSW	7	56,146,669 (GRCm38)	missense	probably benign	0.01
R1633:Herc2	UTSW	7	56,229,369 (GRCm38)	missense	probably null	1.00
R1635:Herc2	UTSW	7	56,136,667 (GRCm38)	missense	probably benign	0.00
R1659:Herc2	UTSW	7	56,135,105 (GRCm38)	missense	probably benign	0.00
R1682:Herc2	UTSW	7	56,088,400 (GRCm38)	missense	possibly damaging	0.87
R1697:Herc2	UTSW	7	56,153,905 (GRCm38)	missense	probably benign	0.43
R1748:Herc2	UTSW	7	56,148,823 (GRCm38)	critical splice donor site	probably null
R1802:Herc2	UTSW	7	56,184,332 (GRCm38)	missense	probably damaging	1.00
R1835:Herc2	UTSW	7	56,206,765 (GRCm38)	nonsense	probably null
R1836:Herc2	UTSW	7	56,155,105 (GRCm38)	nonsense	probably null
R1872:Herc2	UTSW	7	56,157,509 (GRCm38)	missense	probably benign	0.18
R1889:Herc2	UTSW	7	56,189,813 (GRCm38)	missense	possibly damaging	0.60
R1906:Herc2	UTSW	7	56,114,864 (GRCm38)	missense	probably benign	0.01
R2004:Herc2	UTSW	7	56,137,859 (GRCm38)	missense	probably damaging	1.00
R2030:Herc2	UTSW	7	56,184,373 (GRCm38)	missense	probably damaging	0.99
R2037:Herc2	UTSW	7	56,205,961 (GRCm38)	missense	probably damaging	1.00
R2059:Herc2	UTSW	7	56,163,897 (GRCm38)	missense	probably damaging	1.00
R2068:Herc2	UTSW	7	56,132,497 (GRCm38)	missense	probably damaging	1.00
R2072:Herc2	UTSW	7	56,226,964 (GRCm38)	missense	probably damaging	1.00
R2085:Herc2	UTSW	7	56,212,965 (GRCm38)	missense	possibly damaging	0.94
R2115:Herc2	UTSW	7	56,185,828 (GRCm38)	splice site	probably benign
R2160:Herc2	UTSW	7	56,212,922 (GRCm38)	missense	probably benign	0.00
R2173:Herc2	UTSW	7	56,185,951 (GRCm38)	missense	probably benign	0.27
R2221:Herc2	UTSW	7	56,169,018 (GRCm38)	critical splice donor site	probably null
R2280:Herc2	UTSW	7	56,137,271 (GRCm38)	missense	possibly damaging	0.79
R3078:Herc2	UTSW	7	56,137,243 (GRCm38)	missense	probably benign
R3104:Herc2	UTSW	7	56,135,355 (GRCm38)	missense	probably benign	0.23
R3177:Herc2	UTSW	7	56,153,428 (GRCm38)	missense	probably benign	0.00
R3277:Herc2	UTSW	7	56,153,428 (GRCm38)	missense	probably benign	0.00
R3766:Herc2	UTSW	7	56,163,824 (GRCm38)	missense	probably damaging	1.00
R3770:Herc2	UTSW	7	56,165,007 (GRCm38)	missense	probably benign
R3807:Herc2	UTSW	7	56,207,809 (GRCm38)	missense	probably damaging	1.00
R3912:Herc2	UTSW	7	56,098,437 (GRCm38)	missense	probably damaging	0.98
R4004:Herc2	UTSW	7	56,106,465 (GRCm38)	missense	possibly damaging	0.53
R4039:Herc2	UTSW	7	56,156,411 (GRCm38)	missense	probably damaging	0.98
R4190:Herc2	UTSW	7	56,122,448 (GRCm38)	missense	probably benign	0.03
R4225:Herc2	UTSW	7	56,164,987 (GRCm38)	missense	probably damaging	1.00
R4334:Herc2	UTSW	7	56,226,654 (GRCm38)	missense	probably damaging	1.00
R4405:Herc2	UTSW	7	56,170,477 (GRCm38)	missense	probably damaging	1.00
R4448:Herc2	UTSW	7	56,227,892 (GRCm38)	missense	probably damaging	1.00
R4450:Herc2	UTSW	7	56,227,892 (GRCm38)	missense	probably damaging	1.00
R4565:Herc2	UTSW	7	56,153,838 (GRCm38)	missense	possibly damaging	0.71
R4667:Herc2	UTSW	7	56,131,253 (GRCm38)	missense	probably damaging	1.00
R4747:Herc2	UTSW	7	56,106,393 (GRCm38)	missense	possibly damaging	0.80
R4762:Herc2	UTSW	7	56,170,640 (GRCm38)	missense	probably benign	0.19
R4829:Herc2	UTSW	7	56,106,492 (GRCm38)	missense	probably benign	0.39
R4832:Herc2	UTSW	7	56,098,417 (GRCm38)	nonsense	probably null
R4895:Herc2	UTSW	7	56,222,986 (GRCm38)	missense	probably damaging	1.00
R4904:Herc2	UTSW	7	56,157,486 (GRCm38)	missense	probably damaging	0.99
R4908:Herc2	UTSW	7	56,177,912 (GRCm38)	missense	probably benign	0.01
R4911:Herc2	UTSW	7	56,227,892 (GRCm38)	missense	probably damaging	1.00
R4921:Herc2	UTSW	7	56,229,690 (GRCm38)	missense	probably benign	0.04
R4939:Herc2	UTSW	7	56,206,736 (GRCm38)	missense	probably damaging	1.00
R5155:Herc2	UTSW	7	56,227,826 (GRCm38)	missense	possibly damaging	0.85
R5184:Herc2	UTSW	7	56,122,351 (GRCm38)	missense	probably damaging	1.00
R5269:Herc2	UTSW	7	56,168,870 (GRCm38)	nonsense	probably null
R5306:Herc2	UTSW	7	56,184,961 (GRCm38)	missense	probably damaging	1.00
R5314:Herc2	UTSW	7	56,219,786 (GRCm38)	missense	probably damaging	0.99
R5369:Herc2	UTSW	7	56,182,700 (GRCm38)	missense	probably damaging	1.00
R5418:Herc2	UTSW	7	56,137,565 (GRCm38)	missense	probably damaging	1.00
R5420:Herc2	UTSW	7	56,203,830 (GRCm38)	missense	probably damaging	0.96
R5463:Herc2	UTSW	7	56,194,262 (GRCm38)	missense	probably damaging	1.00
R5510:Herc2	UTSW	7	56,206,771 (GRCm38)	missense	probably damaging	1.00
R5634:Herc2	UTSW	7	56,206,783 (GRCm38)	missense	probably damaging	1.00
R5638:Herc2	UTSW	7	56,204,416 (GRCm38)	missense	probably benign	0.01
R5690:Herc2	UTSW	7	56,157,705 (GRCm38)	missense	probably benign
R5762:Herc2	UTSW	7	56,197,190 (GRCm38)	missense	possibly damaging	0.68
R5807:Herc2	UTSW	7	56,230,919 (GRCm38)	missense	probably damaging	0.99
R5878:Herc2	UTSW	7	56,124,248 (GRCm38)	missense	probably benign
R6036:Herc2	UTSW	7	56,068,053 (GRCm38)	missense	probably benign	0.01
R6036:Herc2	UTSW	7	56,068,053 (GRCm38)	missense	probably benign	0.01
R6083:Herc2	UTSW	7	56,228,505 (GRCm38)	missense	probably benign	0.00
R6192:Herc2	UTSW	7	56,207,762 (GRCm38)	missense	probably damaging	1.00
R6193:Herc2	UTSW	7	56,156,901 (GRCm38)	missense	probably damaging	0.98
R6261:Herc2	UTSW	7	56,197,072 (GRCm38)	nonsense	probably null
R6267:Herc2	UTSW	7	56,153,166 (GRCm38)	nonsense	probably null
R6267:Herc2	UTSW	7	56,204,718 (GRCm38)	missense	possibly damaging	0.51
R6298:Herc2	UTSW	7	56,191,265 (GRCm38)	missense	probably benign
R6299:Herc2	UTSW	7	56,135,055 (GRCm38)	missense	possibly damaging	0.47
R6326:Herc2	UTSW	7	56,222,934 (GRCm38)	missense	probably damaging	0.98
R6347:Herc2	UTSW	7	56,194,403 (GRCm38)	critical splice donor site	probably null
R6394:Herc2	UTSW	7	56,215,981 (GRCm38)	missense	probably damaging	1.00
R6500:Herc2	UTSW	7	56,146,645 (GRCm38)	nonsense	probably null
R6526:Herc2	UTSW	7	56,157,330 (GRCm38)	missense	probably damaging	0.99
R6592:Herc2	UTSW	7	56,207,690 (GRCm38)	critical splice acceptor site	probably null
R6619:Herc2	UTSW	7	56,068,092 (GRCm38)	nonsense	probably null
R6719:Herc2	UTSW	7	56,212,826 (GRCm38)	missense	probably damaging	1.00
R6750:Herc2	UTSW	7	56,097,447 (GRCm38)	missense	probably damaging	1.00
R6807:Herc2	UTSW	7	56,164,922 (GRCm38)	missense	probably damaging	1.00
R6811:Herc2	UTSW	7	56,113,433 (GRCm38)	nonsense	probably null
R6837:Herc2	UTSW	7	56,189,841 (GRCm38)	missense	possibly damaging	0.89
R6838:Herc2	UTSW	7	56,108,778 (GRCm38)	missense	probably damaging	1.00
R6902:Herc2	UTSW	7	56,135,486 (GRCm38)	missense	probably benign	0.37
R6983:Herc2	UTSW	7	56,106,453 (GRCm38)	missense	possibly damaging	0.74
R6985:Herc2	UTSW	7	56,132,480 (GRCm38)	missense	probably damaging	1.00
R6985:Herc2	UTSW	7	56,106,453 (GRCm38)	missense	possibly damaging	0.74
R6986:Herc2	UTSW	7	56,106,453 (GRCm38)	missense	possibly damaging	0.74
R6987:Herc2	UTSW	7	56,106,453 (GRCm38)	missense	possibly damaging	0.74
R7113:Herc2	UTSW	7	56,203,849 (GRCm38)	missense	probably damaging	0.99
R7173:Herc2	UTSW	7	56,203,827 (GRCm38)	missense	probably damaging	1.00
R7202:Herc2	UTSW	7	56,131,286 (GRCm38)	missense	probably damaging	0.99
R7205:Herc2	UTSW	7	56,182,640 (GRCm38)	missense	probably damaging	1.00
R7236:Herc2	UTSW	7	56,085,080 (GRCm38)	missense	probably benign	0.29
R7297:Herc2	UTSW	7	56,136,658 (GRCm38)	missense	probably benign	0.00
R7358:Herc2	UTSW	7	56,182,675 (GRCm38)	missense	possibly damaging	0.48
R7438:Herc2	UTSW	7	56,103,718 (GRCm38)	splice site	probably null
R7537:Herc2	UTSW	7	56,219,779 (GRCm38)	nonsense	probably null
R7578:Herc2	UTSW	7	56,134,800 (GRCm38)	missense	probably benign	0.07
R7614:Herc2	UTSW	7	56,153,275 (GRCm38)	nonsense	probably null
R7638:Herc2	UTSW	7	56,220,525 (GRCm38)	missense	probably damaging	1.00
R7638:Herc2	UTSW	7	56,157,438 (GRCm38)	missense	probably benign	0.26
R7646:Herc2	UTSW	7	56,134,613 (GRCm38)	missense	probably benign
R7663:Herc2	UTSW	7	56,136,685 (GRCm38)	missense	probably benign
R7665:Herc2	UTSW	7	56,153,155 (GRCm38)	missense	probably damaging	1.00
R7691:Herc2	UTSW	7	56,191,845 (GRCm38)	missense	probably benign
R7733:Herc2	UTSW	7	56,188,664 (GRCm38)	missense	probably damaging	0.99
R7767:Herc2	UTSW	7	56,228,527 (GRCm38)	missense	probably benign	0.39
R7802:Herc2	UTSW	7	56,164,090 (GRCm38)	missense	probably damaging	1.00
R7847:Herc2	UTSW	7	56,157,560 (GRCm38)	critical splice donor site	probably null
R7956:Herc2	UTSW	7	56,113,400 (GRCm38)	missense	probably damaging	0.97
R7985:Herc2	UTSW	7	56,165,244 (GRCm38)	missense	probably benign
R8003:Herc2	UTSW	7	56,168,904 (GRCm38)	missense	possibly damaging	0.94
R8045:Herc2	UTSW	7	56,184,900 (GRCm38)	missense	probably damaging	1.00
R8085:Herc2	UTSW	7	56,229,679 (GRCm38)	missense	probably benign	0.01
R8134:Herc2	UTSW	7	56,085,136 (GRCm38)	missense	probably benign	0.10
R8259:Herc2	UTSW	7	56,205,890 (GRCm38)	missense	probably damaging	0.99
R8286:Herc2	UTSW	7	56,229,662 (GRCm38)	missense	possibly damaging	0.92
R8304:Herc2	UTSW	7	56,159,438 (GRCm38)	missense	probably damaging	1.00
R8321:Herc2	UTSW	7	56,229,348 (GRCm38)	missense	possibly damaging	0.84
R8332:Herc2	UTSW	7	56,146,595 (GRCm38)	missense	probably damaging	1.00
R8432:Herc2	UTSW	7	56,155,112 (GRCm38)	missense	probably benign	0.14
R8516:Herc2	UTSW	7	56,206,570 (GRCm38)	missense	probably benign	0.05
R8738:Herc2	UTSW	7	56,148,654 (GRCm38)	missense	possibly damaging	0.78
R8742:Herc2	UTSW	7	56,094,395 (GRCm38)	missense	probably benign	0.12
R8796:Herc2	UTSW	7	56,135,375 (GRCm38)	missense	probably benign	0.01
R8825:Herc2	UTSW	7	56,050,878 (GRCm38)	start codon destroyed	probably null	0.01
R8826:Herc2	UTSW	7	56,106,396 (GRCm38)	missense	probably benign	0.12
R8842:Herc2	UTSW	7	56,088,311 (GRCm38)	missense	probably damaging	0.99
R9103:Herc2	UTSW	7	56,135,055 (GRCm38)	missense	possibly damaging	0.47
R9124:Herc2	UTSW	7	56,184,308 (GRCm38)	missense	probably damaging	1.00
R9134:Herc2	UTSW	7	56,182,429 (GRCm38)	missense	probably damaging	0.99
R9168:Herc2	UTSW	7	56,152,460 (GRCm38)	missense	probably damaging	0.99
R9173:Herc2	UTSW	7	56,206,602 (GRCm38)	missense	probably damaging	0.97
R9238:Herc2	UTSW	7	56,163,760 (GRCm38)	missense	probably damaging	0.98
R9249:Herc2	UTSW	7	56,113,142 (GRCm38)	missense	probably damaging	1.00
R9344:Herc2	UTSW	7	56,122,364 (GRCm38)	missense	probably benign	0.07
R9432:Herc2	UTSW	7	56,131,184 (GRCm38)	missense	probably damaging	1.00
R9472:Herc2	UTSW	7	56,164,095 (GRCm38)	missense	probably damaging	1.00
R9513:Herc2	UTSW	7	56,113,100 (GRCm38)	missense	probably damaging	1.00
R9579:Herc2	UTSW	7	56,108,752 (GRCm38)	missense	probably damaging	0.99
R9596:Herc2	UTSW	7	56,184,847 (GRCm38)	missense
R9664:Herc2	UTSW	7	56,170,590 (GRCm38)	missense	possibly damaging	0.90
R9760:Herc2	UTSW	7	56,163,911 (GRCm38)	critical splice donor site	probably null
R9781:Herc2	UTSW	7	56,100,348 (GRCm38)	missense	possibly damaging	0.53
RF024:Herc2	UTSW	7	56,226,525 (GRCm38)	missense	probably damaging	1.00
X0011:Herc2	UTSW	7	56,131,292 (GRCm38)	missense	probably benign
X0023:Herc2	UTSW	7	56,090,918 (GRCm38)	missense	possibly damaging	0.73
X0057:Herc2	UTSW	7	56,229,690 (GRCm38)	missense	probably benign	0.04
X0064:Herc2	UTSW	7	56,191,258 (GRCm38)	missense	probably benign
X0064:Herc2	UTSW	7	56,191,211 (GRCm38)	missense	probably benign	0.01
Z1088:Herc2	UTSW	7	56,131,292 (GRCm38)	missense	probably benign
Z1088:Herc2	UTSW	7	56,087,341 (GRCm38)	missense	probably benign	0.00
Z1088:Herc2	UTSW	7	56,226,589 (GRCm38)	missense	probably damaging	1.00
Z1088:Herc2	UTSW	7	56,215,432 (GRCm38)	missense	probably damaging	1.00
Z1088:Herc2	UTSW	7	56,215,381 (GRCm38)	missense	possibly damaging	0.86
Z1176:Herc2	UTSW	7	56,131,292 (GRCm38)	missense	probably benign
Z1176:Herc2	UTSW	7	56,097,533 (GRCm38)	missense	possibly damaging	0.48
Z1176:Herc2	UTSW	7	56,132,498 (GRCm38)	missense	probably damaging	1.00
Z1177:Herc2	UTSW	7	56,131,292 (GRCm38)	missense	probably benign
Z1177:Herc2	UTSW	7	56,121,589 (GRCm38)	missense	possibly damaging	0.55

Predicted Primers

PCR Primer
(F):5'- TTCCCTCAGAACTGTATGTACTCATG -3'
(R):5'- TGTGTTACCTAAGTAAGAAGCACC -3'

Sequencing Primer
(F):5'- CAGAACTGTATGTACTCATGTTCTC -3'
(R):5'- GTTACCTAAGTAAGAAGCACCTAGCG -3'

Posted On

2021-03-08