Mutagenetix > Incidental Mutation

Incidental Mutation 'R8677:Miip'

661436

Institutional Source

Beutler Lab

Gene Symbol

Miip

Ensembl Gene

ENSMUSG00000029022

Gene Name

migration and invasion inhibitory protein

Synonyms

D4Wsu114e

MMRRC Submission

068532-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

R8677 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

147945235-147953176 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 147947503 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Tyrosine at position 219 (C219Y)

Ref Sequence

ENSEMBL: ENSMUSP00000113897 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030886] [ENSMUST00000119975] [ENSMUST00000172710]

AlphaFold

A2A7Y5

Predicted Effect

probably damaging
Transcript: ENSMUST00000030886
AA Change: C219Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000030886
Gene: ENSMUSG00000029022
AA Change: C219Y

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000119975
AA Change: C219Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000113897
Gene: ENSMUSG00000029022
AA Change: C219Y

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
Pfam:MIIP	41	382	1.4e-142	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000172710
AA Change: C219Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000134085
Gene: ENSMUSG00000029022
AA Change: C219Y

Domain	Start	End	E-Value	Type
low complexity region	13	24	N/A	INTRINSIC
low complexity region	60	69	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that interacts with the oncogene protein insulin-like growth factor binding protein 2 and may function as an inhibitor of cell migration and invasion. This protein also interacts with the cell division protein 20 and may be involved in regulating mitotic progression. This protein may function as a tumor suppressor by inhibiting the growth or certain cancers. [provided by RefSeq, Sep 2011]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acd	A	C	8: 106,427,576 (GRCm39)	S25R	probably damaging	Het
Acly	A	T	11: 100,410,569 (GRCm39)	H136Q	probably damaging	Het
Adrm1	C	T	2: 179,813,832 (GRCm39)	T2M	probably benign	Het
Ankrd12	A	T	17: 66,331,209 (GRCm39)	L246M	probably damaging	Het
Bltp2	C	T	11: 78,174,982 (GRCm39)	R1706C	probably damaging	Het
Cacnb3	T	C	15: 98,539,931 (GRCm39)	L258P	probably damaging	Het
Cd36	C	T	5: 18,025,493 (GRCm39)	V76M	probably damaging	Het
Cep120	A	G	18: 53,871,633 (GRCm39)	F80L	possibly damaging	Het
Clcn1	A	T	6: 42,267,519 (GRCm39)		probably null	Het
Col17a1	T	C	19: 47,640,240 (GRCm39)	T1042A	probably benign	Het
Comp	A	T	8: 70,832,910 (GRCm39)	N623Y	probably damaging	Het
Ctnnal1	A	G	4: 56,813,272 (GRCm39)	L653P	probably benign	Het
Cx3cl1	G	T	8: 95,506,443 (GRCm39)	R149S	probably benign	Het
Dbndd2	T	A	2: 164,330,522 (GRCm39)	N58K	probably damaging	Het
Dclk1	A	G	3: 55,409,840 (GRCm39)	I595V	probably damaging	Het
Dhx15	T	C	5: 52,341,886 (GRCm39)	D144G	probably benign	Het
Dlat	T	C	9: 50,570,007 (GRCm39)	E120G	probably damaging	Het
Fam171a1	T	A	2: 3,221,352 (GRCm39)	Y273N	probably damaging	Het
Fndc3b	A	T	3: 27,511,176 (GRCm39)	V778D	probably benign	Het
Grn	T	C	11: 102,324,393 (GRCm39)	S129P	possibly damaging	Het
Grxcr1	T	C	5: 68,267,757 (GRCm39)	F169L	possibly damaging	Het
Hcn2	A	G	10: 79,560,619 (GRCm39)	I317V	probably benign	Het
Heca	T	C	10: 17,791,424 (GRCm39)	N211D	probably benign	Het
Htr1f	T	C	16: 64,746,414 (GRCm39)	T293A	possibly damaging	Het
Ifitm10	T	A	7: 141,909,749 (GRCm39)	I116F	probably benign	Het
Itprid1	A	G	6: 55,849,579 (GRCm39)	H37R	probably benign	Het
Ivl	A	T	3: 92,479,986 (GRCm39)	D26E	probably benign	Het
Kcnma1	T	A	14: 23,436,418 (GRCm39)	E761V	probably benign	Het
Ltbp1	G	T	17: 75,655,753 (GRCm39)	V923F	probably benign	Het
Mfsd4b2	A	T	10: 39,799,805 (GRCm39)	F32L	probably benign	Het
Micu2	T	C	14: 58,161,420 (GRCm39)	R301G	possibly damaging	Het
Mn1	T	A	5: 111,566,885 (GRCm39)	L285*	probably null	Het
Mthfd1l	T	A	10: 3,998,250 (GRCm39)	N664K	possibly damaging	Het
Mttp	A	T	3: 137,810,437 (GRCm39)	H659Q	probably benign	Het
Myadml2	G	A	11: 120,538,815 (GRCm39)	P7S	probably benign	Het
Nlgn3	T	C	X: 100,352,390 (GRCm39)	V179A	probably damaging	Het
Nlrp4c	C	T	7: 6,075,644 (GRCm39)	T645I	probably damaging	Het
Notch1	C	T	2: 26,359,936 (GRCm39)	V1260I	probably damaging	Het
Numa1	T	C	7: 101,650,148 (GRCm39)	L1293P	probably damaging	Het
Or1a1	A	T	11: 74,086,415 (GRCm39)	I29F	probably benign	Het
Or56a4	A	G	7: 104,806,775 (GRCm39)	L38P	probably benign	Het
Pcdh12	G	T	18: 38,415,191 (GRCm39)	H645N	probably benign	Het
Pdcd1	A	T	1: 93,968,952 (GRCm39)	L122H	probably damaging	Het
Pknox2	G	A	9: 36,821,887 (GRCm39)	P246L	probably damaging	Het
Polr2a	A	T	11: 69,626,381 (GRCm39)	S1590T	possibly damaging	Het
Ppp1r16b	G	A	2: 158,593,098 (GRCm39)	D226N	probably damaging	Het
Proser1	A	G	3: 53,385,122 (GRCm39)	T335A	probably benign	Het
Ptprc	A	G	1: 138,011,335 (GRCm39)	I598T	probably damaging	Het
Rasal3	T	C	17: 32,615,828 (GRCm39)	T337A	probably benign	Het
Rasgrp3	A	T	17: 75,819,055 (GRCm39)	T415S	probably benign	Het
Rbm7	T	A	9: 48,401,273 (GRCm39)	R152*	probably null	Het
Sanbr	T	C	11: 23,545,471 (GRCm39)	D474G	probably benign	Het
Sf3b2	C	T	19: 5,336,257 (GRCm39)	R513H	probably damaging	Het
Slc22a30	A	G	19: 8,364,035 (GRCm39)	S214P	probably benign	Het
Spam1	A	G	6: 24,796,984 (GRCm39)	T312A	probably benign	Het
Ssh2	A	G	11: 77,346,019 (GRCm39)	T1335A	possibly damaging	Het
Tef	T	A	15: 81,699,169 (GRCm39)	L59Q	probably damaging	Het
Tmem131l	A	C	3: 83,836,009 (GRCm39)	V700G	probably damaging	Het
Twsg1	A	G	17: 66,233,402 (GRCm39)	S183P	probably damaging	Het
Unc13c	T	C	9: 73,840,243 (GRCm39)	T203A	probably benign	Het
Uqcc6	G	T	10: 82,456,050 (GRCm39)	T37K	possibly damaging	Het
Vmn2r75	G	T	7: 85,814,410 (GRCm39)	P361Q	possibly damaging	Het
Xirp2	A	T	2: 67,346,978 (GRCm39)	N3073I	probably damaging	Het
Zfp114	A	G	7: 23,880,070 (GRCm39)	N140D	probably benign	Het

Other mutations in Miip

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00531:Miip	APN	4	147,950,322 (GRCm39)	missense	probably damaging	1.00
IGL02134:Miip	APN	4	147,949,735 (GRCm39)	splice site	probably benign
IGL02829:Miip	APN	4	147,947,518 (GRCm39)	missense	probably benign	0.01
IGL03350:Miip	APN	4	147,946,979 (GRCm39)	missense	probably benign	0.01
R0200:Miip	UTSW	4	147,946,720 (GRCm39)	missense	probably damaging	0.99
R1647:Miip	UTSW	4	147,949,691 (GRCm39)	missense	probably benign	0.02
R1783:Miip	UTSW	4	147,950,231 (GRCm39)	missense	probably damaging	1.00
R1848:Miip	UTSW	4	147,947,549 (GRCm39)	missense	probably damaging	0.99
R1944:Miip	UTSW	4	147,950,422 (GRCm39)	missense	probably benign	0.15
R3615:Miip	UTSW	4	147,950,371 (GRCm39)	missense	probably benign	0.00
R3616:Miip	UTSW	4	147,950,371 (GRCm39)	missense	probably benign	0.00
R3882:Miip	UTSW	4	147,945,509 (GRCm39)	missense	possibly damaging	0.93
R4579:Miip	UTSW	4	147,945,518 (GRCm39)	missense	probably damaging	1.00
R5183:Miip	UTSW	4	147,947,526 (GRCm39)	missense	probably damaging	1.00
R6054:Miip	UTSW	4	147,950,135 (GRCm39)	missense	probably benign	0.00
R6056:Miip	UTSW	4	147,946,792 (GRCm39)	missense	probably damaging	1.00
R6304:Miip	UTSW	4	147,947,540 (GRCm39)	missense	probably benign	0.12
R6568:Miip	UTSW	4	147,950,372 (GRCm39)	missense	probably benign
R6603:Miip	UTSW	4	147,950,380 (GRCm39)	missense	possibly damaging	0.92
R7639:Miip	UTSW	4	147,947,021 (GRCm39)	missense	probably benign	0.22
R7701:Miip	UTSW	4	147,947,371 (GRCm39)	missense	probably null	0.86
R7795:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R7796:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R7797:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R7872:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R7920:Miip	UTSW	4	147,947,375 (GRCm39)	missense	probably benign	0.17
R8468:Miip	UTSW	4	147,945,928 (GRCm39)	missense	probably damaging	1.00
R8492:Miip	UTSW	4	147,945,881 (GRCm39)	missense	probably damaging	1.00
R8852:Miip	UTSW	4	147,950,839 (GRCm39)	start gained	probably benign
R8860:Miip	UTSW	4	147,950,839 (GRCm39)	start gained	probably benign
R9755:Miip	UTSW	4	147,950,319 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TGCTTAGATACCAGGCTGCC -3'
(R):5'- TGGTGCACAAACAGAGCTGG -3'

Sequencing Primer
(F):5'- TTCCTGAGTCCACAGCTCAGAG -3'
(R):5'- CTGGCACTAACAGAGGGTG -3'

Posted On

2021-03-08