Mutagenetix > Incidental Mutation

Incidental Mutation 'R8682:Mslnl'

661843

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

068537-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8682 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 25746988 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 612 (D612G)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098] [ENSMUST00000075884]

AlphaFold

Q8C160

Predicted Effect

probably benign
Transcript: ENSMUST00000047098
AA Change: D612G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: D612G

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000075884

SMART Domains

Protein: ENSMUSP00000075279
Gene: ENSMUSG00000063011

Domain	Start	End	E-Value	Type
Pfam:Mesothelin	1	624	N/A	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 98.9%
20x: 95.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810065E05Rik	T	A	11: 58,423,899	L141Q	probably null	Het
Abtb2	A	T	2: 103,567,375	T217S	probably benign	Het
Adcy1	T	C	11: 7,161,328	I873T	probably damaging	Het
Angpt4	A	G	2: 151,927,085	M172V	probably benign	Het
Apaf1	A	T	10: 90,995,670	V1194D	probably damaging	Het
Arhgap30	T	C	1: 171,407,402	S479P	probably benign	Het
Asah2	C	T	19: 32,052,877	V132M	probably damaging	Het
Bmp10	A	G	6: 87,433,559		probably null	Het
Bsn	T	C	9: 108,106,169	Y790C		Het
Cacna2d1	C	A	5: 16,353,839	R732S	possibly damaging	Het
Casr	A	T	16: 36,495,422	F762Y	possibly damaging	Het
Cbln1	T	C	8: 87,472,107	D45G	possibly damaging	Het
Cd177	A	T	7: 24,760,013	M61K	possibly damaging	Het
Cdh11	T	C	8: 102,650,716	I433V	probably benign	Het
Cdhr5	A	G	7: 141,275,986		probably null	Het
Col12a1	T	A	9: 79,661,076	K1622I	probably benign	Het
Cyp2d9	A	G	15: 82,453,716	D103G	probably damaging	Het
Fem1b	A	T	9: 62,797,150	L276*	probably null	Het
Fggy	T	A	4: 95,812,121	V343E	probably damaging	Het
Flt3	A	G	5: 147,383,455	V33A	probably benign	Het
Gm6904	G	A	14: 59,258,584	T27I	probably benign	Het
Gm8994	A	G	6: 136,329,029	T163A	possibly damaging	Het
Grn	T	C	11: 102,434,820	Y288H	probably benign	Het
Grtp1	G	A	8: 13,179,499	R272W	probably damaging	Het
H2-Aa	A	G	17: 34,283,760	I144T	possibly damaging	Het
Herc1	A	G	9: 66,462,848	D469G		Het
Hsf2	G	A	10: 57,505,171	E286K	possibly damaging	Het
Hydin	A	G	8: 110,309,166	E163G	probably damaging	Het
Il6ra	A	G	3: 89,886,669	I224T	possibly damaging	Het
Itih3	T	A	14: 30,920,716	I204F	possibly damaging	Het
Kcnj9	A	T	1: 172,326,113	M148K	possibly damaging	Het
Lepr	T	G	4: 101,792,072	V890G	probably benign	Het
Myl2	T	A	5: 122,106,735	V156D	probably damaging	Het
Neb	C	A	2: 52,246,845	W3208L	probably damaging	Het
Neto2	T	C	8: 85,640,666	Y511C	probably benign	Het
Obox2	C	A	7: 15,396,987	T48K	possibly damaging	Het
Olfr1109	A	T	2: 87,093,046	M117K	possibly damaging	Het
Olfr305	C	T	7: 86,364,165	M57I	probably damaging	Het
Olfr568	T	A	7: 102,877,439	F106L	probably benign	Het
Osbpl6	C	G	2: 76,577,081	H486D	probably benign	Het
Pfkfb3	T	C	2: 11,484,333	K264E	probably benign	Het
Pla2r1	T	A	2: 60,422,776	T1324S	possibly damaging	Het
Plekhg1	A	T	10: 3,947,523	Y495F		Het
Ppp2r5d	T	C	17: 46,687,063	K225E	probably benign	Het
Ptpn11	T	C	5: 121,167,990	D64G	possibly damaging	Het
Ptprs	A	G	17: 56,435,849	I431T	probably damaging	Het
Rapgef5	T	C	12: 117,581,812	S100P	probably benign	Het
Rnf219	G	T	14: 104,480,233	R235S	probably damaging	Het
Shh	T	A	5: 28,458,060	H370L	probably benign	Het
Siah3	A	T	14: 75,525,603	H98L	possibly damaging	Het
Sim2	C	T	16: 94,123,333	H446Y	probably benign	Het
Skint4	T	C	4: 112,136,040	I320T	possibly damaging	Het
Sorcs1	A	T	19: 50,378,960	N221K	probably damaging	Het
Sox6	T	C	7: 115,476,956	S816G	probably damaging	Het
Sphkap	T	C	1: 83,279,276	T251A	probably benign	Het
Stard9	T	C	2: 120,703,315	V3351A	possibly damaging	Het
Tbc1d15	A	G	10: 115,210,290	V436A	probably benign	Het
Thsd7b	A	T	1: 129,760,274	K641*	probably null	Het
Tmc5	G	T	7: 118,670,702	V892F	possibly damaging	Het
Tpsab1	T	A	17: 25,343,711	H238L	probably benign	Het
Trank1	A	G	9: 111,365,344	N812S	probably benign	Het
Trio	T	A	15: 27,905,192	N163Y	unknown	Het
Ttc39d	A	G	17: 80,217,264	T451A	probably benign	Het
Ube3b	T	C	5: 114,412,290	L832P	probably damaging	Het
Vmn2r15	C	A	5: 109,294,072	C165F	probably damaging	Het
Vmn2r90	T	C	17: 17,712,082	F84L	possibly damaging	Het
Wdr60	A	T	12: 116,224,990	H661Q	probably damaging	Het
Wdr95	C	T	5: 149,595,287	T531I	possibly damaging	Het
Zcrb1	C	A	15: 93,386,237	G191V	probably benign	Het
Zfp111	G	A	7: 24,198,558	P544S	probably damaging	Het
Zfyve9	T	C	4: 108,719,342	S181G	probably benign	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25743667	unclassified	probably benign
IGL01629:Mslnl	APN	17	25744775	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25746151	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25747998	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25744103	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25744077	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25743203	nonsense	probably null
R0881:Mslnl	UTSW	17	25742965	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25744517	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25744969	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25738978	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25738968	nonsense	probably null
R5257:Mslnl	UTSW	17	25746165	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25743159	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25737842	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25746775	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25737902	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25744557	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25746073	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25743212	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25743210	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25736921	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25743183	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25746777	missense	probably benign	0.03
R8735:Mslnl	UTSW	17	25745088	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25745073	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25742720	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25742532	intron	probably benign
RF007:Mslnl	UTSW	17	25743228	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- CTTCATTTGCAGAGCATGGGAG -3'
(R):5'- AGGAAATTGTTTCACCATAGATGG -3'

Sequencing Primer
(F):5'- AATCTGCTGGGCCAGAAC -3'
(R):5'- CCATAGATGGTTCATTAGGCAATGAC -3'

Posted On

2021-03-08