Mutagenetix > Incidental Mutation

Incidental Mutation 'R8684:Zfp101'

661966

Institutional Source

Beutler Lab

Gene Symbol

Zfp101

Ensembl Gene

ENSMUSG00000055240

Gene Name

zinc finger protein 101

Synonyms

MMRRC Submission

068539-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R8684 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

33599148-33613593 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 33600977 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 260 (S260P)

Ref Sequence

ENSEMBL: ENSMUSP00000132508 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167107] [ENSMUST00000174417] [ENSMUST00000174512]

AlphaFold

Q8BP18

Predicted Effect

possibly damaging
Transcript: ENSMUST00000167107
AA Change: S260P

PolyPhen 2 Score 0.856 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000132508
Gene: ENSMUSG00000055240
AA Change: S260P

Domain	Start	End	E-Value	Type
KRAB	10	62	9.34e-15	SMART
ZnF_C2H2	219	241	2.57e-3	SMART
ZnF_C2H2	247	269	6.32e-3	SMART
ZnF_C2H2	275	297	3.16e-3	SMART
ZnF_C2H2	302	322	1.65e2	SMART
ZnF_C2H2	330	352	6.75e0	SMART
ZnF_C2H2	358	380	2.75e-3	SMART
ZnF_C2H2	386	408	4.47e-3	SMART
ZnF_C2H2	414	436	2.09e-3	SMART
ZnF_C2H2	442	464	4.79e-3	SMART
ZnF_C2H2	470	492	2.2e-2	SMART
ZnF_C2H2	498	520	4.47e-3	SMART
ZnF_C2H2	526	548	4.72e-2	SMART
ZnF_C2H2	554	576	1.28e-3	SMART
ZnF_C2H2	583	605	5.06e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000174417

SMART Domains

Protein: ENSMUSP00000134459
Gene: ENSMUSG00000055240

Domain	Start	End	E-Value	Type
Blast:KRAB	1	38	5e-16	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000174512

SMART Domains

Protein: ENSMUSP00000133873
Gene: ENSMUSG00000055240

Domain	Start	End	E-Value	Type
KRAB	10	62	9.34e-15	SMART

Meta Mutation Damage Score

0.1712

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

100% (47/47)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933421I07Rik	C	T	7: 42,097,413 (GRCm39)	R27H	probably benign	Het
Abca2	T	A	2: 25,336,508 (GRCm39)	L2229Q	possibly damaging	Het
Adamts4	A	T	1: 171,086,541 (GRCm39)	T778S	probably damaging	Het
AY358078	T	G	14: 52,059,597 (GRCm39)	Y311*	probably null	Het
Catsperg1	C	T	7: 28,897,825 (GRCm39)	V181M	probably damaging	Het
Cdcp3	C	A	7: 130,837,688 (GRCm39)	S528*	probably null	Het
Cep70	A	G	9: 99,145,842 (GRCm39)	K170E	possibly damaging	Het
Coro1b	T	A	19: 4,199,527 (GRCm39)	V62E	probably damaging	Het
Dnajc11	T	C	4: 152,065,183 (GRCm39)	*560Q	probably null	Het
Eci3	T	C	13: 35,143,874 (GRCm39)	N84D	probably damaging	Het
F5	T	A	1: 164,045,111 (GRCm39)	V2133E	probably benign	Het
Fancl	C	A	11: 26,420,826 (GRCm39)	P116Q		Het
Gm10801	ATTTTCAGTTTTCTTGCCATATTCCACGTCCTGCACTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTC	ATTTTCAGTTTTC	2: 98,492,669 (GRCm39)		probably null	Het
Golgb1	C	T	16: 36,734,764 (GRCm39)	T1378M	possibly damaging	Het
Hdac5	T	C	11: 102,096,147 (GRCm39)	N342S	probably benign	Het
Herc3	A	G	6: 58,864,561 (GRCm39)	K732E	probably damaging	Het
Lce1e	A	T	3: 92,615,269 (GRCm39)	I26N	unknown	Het
Lingo1	T	C	9: 56,528,106 (GRCm39)	Y167C	probably damaging	Het
Loxl3	A	G	6: 83,012,566 (GRCm39)	E35G	probably benign	Het
Mmp13	A	G	9: 7,282,089 (GRCm39)	M464V	possibly damaging	Het
Mov10	T	C	3: 104,711,690 (GRCm39)	H199R	probably benign	Het
Nbas	A	G	12: 13,386,368 (GRCm39)	T765A	probably damaging	Het
Nlgn3	C	T	X: 100,363,425 (GRCm39)	R679*	probably null	Het
Nrp1	C	T	8: 129,085,885 (GRCm39)		probably benign	Het
Nup88	C	A	11: 70,860,687 (GRCm39)	V31L	probably benign	Het
Or2r11	T	C	6: 42,437,827 (GRCm39)	N42S	probably damaging	Het
Or5d39	T	C	2: 87,979,872 (GRCm39)	T164A	probably benign	Het
Or7a36	A	T	10: 78,820,212 (GRCm39)	D196V	probably benign	Het
Pah	A	G	10: 87,414,827 (GRCm39)	N393S	probably benign	Het
Peli3	T	C	19: 4,985,022 (GRCm39)	Y163C	probably damaging	Het
Rasgef1b	A	T	5: 99,524,994 (GRCm39)	M55K	probably benign	Het
Ror2	T	C	13: 53,264,302 (GRCm39)	D930G	possibly damaging	Het
Rpgrip1l	T	G	8: 92,000,329 (GRCm39)	M537L	probably benign	Het
Ryr2	A	T	13: 11,702,875 (GRCm39)	V2871E	probably damaging	Het
Smco1	A	T	16: 32,092,841 (GRCm39)	N171Y	probably damaging	Het
Sorbs1	G	C	19: 40,365,244 (GRCm39)	R180G	probably benign	Het
Sox2	T	A	3: 34,705,016 (GRCm39)	L151Q	probably benign	Het
Spink5	A	G	18: 44,143,305 (GRCm39)	E754G	probably benign	Het
Sulf1	A	T	1: 12,867,004 (GRCm39)	M63L	probably benign	Het
Traf2	A	G	2: 25,410,458 (GRCm39)	M390T	probably damaging	Het
Trav13d-4	T	C	14: 53,310,266 (GRCm39)	V16A	probably damaging	Het
Trmo	C	T	4: 46,386,251 (GRCm39)	W84*	probably null	Het
Trmo	T	C	4: 46,386,253 (GRCm39)		probably null	Het
Ttyh1	T	A	7: 4,133,791 (GRCm39)		probably benign	Het
Ush2a	A	G	1: 188,643,220 (GRCm39)	N4194S	possibly damaging	Het
Vmn2r45	T	C	7: 8,486,511 (GRCm39)	Y259C	probably damaging	Het
Vmn2r94	T	A	17: 18,497,912 (GRCm39)		probably benign	Het

Other mutations in Zfp101

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0189:Zfp101	UTSW	17	33,601,213 (GRCm39)	missense	possibly damaging	0.53
R0254:Zfp101	UTSW	17	33,599,952 (GRCm39)	missense	possibly damaging	0.71
R0448:Zfp101	UTSW	17	33,601,295 (GRCm39)	missense	possibly damaging	0.73
R0696:Zfp101	UTSW	17	33,600,401 (GRCm39)	missense	possibly damaging	0.86
R0834:Zfp101	UTSW	17	33,601,418 (GRCm39)	missense	probably benign	0.33
R2000:Zfp101	UTSW	17	33,600,491 (GRCm39)	missense	possibly damaging	0.92
R2366:Zfp101	UTSW	17	33,599,972 (GRCm39)	missense	probably benign	0.06
R2471:Zfp101	UTSW	17	33,599,950 (GRCm39)	missense	possibly damaging	0.85
R3713:Zfp101	UTSW	17	33,600,880 (GRCm39)	missense	probably benign	0.05
R3857:Zfp101	UTSW	17	33,601,405 (GRCm39)	nonsense	probably null
R4537:Zfp101	UTSW	17	33,601,466 (GRCm39)	missense	possibly damaging	0.87
R4712:Zfp101	UTSW	17	33,613,457 (GRCm39)	splice site	probably null
R5049:Zfp101	UTSW	17	33,600,872 (GRCm39)	missense	possibly damaging	0.71
R5241:Zfp101	UTSW	17	33,601,210 (GRCm39)	missense	probably benign
R5499:Zfp101	UTSW	17	33,601,318 (GRCm39)	missense	probably benign	0.20
R5587:Zfp101	UTSW	17	33,600,295 (GRCm39)	missense	possibly damaging	0.71
R5694:Zfp101	UTSW	17	33,599,919 (GRCm39)	missense	probably benign
R5994:Zfp101	UTSW	17	33,599,936 (GRCm39)	missense	probably benign
R6193:Zfp101	UTSW	17	33,600,720 (GRCm39)	nonsense	probably null
R7331:Zfp101	UTSW	17	33,601,559 (GRCm39)	missense	possibly damaging	0.53
R7393:Zfp101	UTSW	17	33,605,674 (GRCm39)	missense	possibly damaging	0.71
R7434:Zfp101	UTSW	17	33,600,564 (GRCm39)	missense	possibly damaging	0.86
R7922:Zfp101	UTSW	17	33,600,511 (GRCm39)	missense	possibly damaging	0.93
R8087:Zfp101	UTSW	17	33,599,977 (GRCm39)	missense	probably benign
R9443:Zfp101	UTSW	17	33,601,418 (GRCm39)	missense	probably benign	0.33
R9581:Zfp101	UTSW	17	33,605,730 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ACAATGTGTGCATGAATATGGC -3'
(R):5'- AGTGTACATCTGACCAGATCGATG -3'

Sequencing Primer
(F):5'- GCATGAATATGGCTTGTCTCC -3'
(R):5'- GATCGATGAAAAACTCCACAGTG -3'

Posted On

2021-03-08