Mutagenetix > Incidental Mutation

Incidental Mutation 'R8721:Tas2r134'

662104

Institutional Source

Beutler Lab

Gene Symbol

Tas2r134

Ensembl Gene

ENSMUSG00000056115

Gene Name

taste receptor, type 2, member 134

Synonyms

Tas2r34, T2R134

MMRRC Submission

068717-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.056) question?

Stock #

R8721 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

51517523-51518419 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 51517571 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 17 (S17P)

Ref Sequence

ENSEMBL: ENSMUSP00000064657 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000070028]

AlphaFold

Q7TQB0

Predicted Effect

probably damaging
Transcript: ENSMUST00000070028
AA Change: S17P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000064657
Gene: ENSMUSG00000056115
AA Change: S17P

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	291	1.4e-75	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.1%
20x: 96.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca9	T	C	11: 110,035,115 (GRCm39)	D684G	possibly damaging	Het
Abhd16a	T	C	17: 35,310,571 (GRCm39)	V94A	possibly damaging	Het
Acly	A	T	11: 100,412,806 (GRCm39)		probably null	Het
Adamts1	G	T	16: 85,594,775 (GRCm39)	T621K	probably damaging	Het
Arhgap24	A	T	5: 103,023,565 (GRCm39)	T200S	possibly damaging	Het
Arhgap31	A	G	16: 38,427,058 (GRCm39)	V598A	probably benign	Het
Ascc1	T	C	10: 59,933,928 (GRCm39)	F323S	possibly damaging	Het
Cdcp3	T	C	7: 130,879,335 (GRCm39)	S1785P	unknown	Het
Ces2b	T	A	8: 105,560,527 (GRCm39)	V114D	possibly damaging	Het
Cfap73	T	C	5: 120,768,089 (GRCm39)	E223G	probably benign	Het
Csnk1e	T	C	15: 79,314,015 (GRCm39)	N78S	possibly damaging	Het
Cyp7a1	G	T	4: 6,268,273 (GRCm39)	A484E	probably damaging	Het
Dnah9	G	T	11: 65,986,124 (GRCm39)	T1170K	probably damaging	Het
Fam83d	G	T	2: 158,627,522 (GRCm39)	V404L	probably benign	Het
Fbxw28	C	T	9: 109,157,382 (GRCm39)	V289I	probably benign	Het
Frs2	T	G	10: 116,909,935 (GRCm39)	I476L	probably benign	Het
Gbp2b	A	T	3: 142,312,705 (GRCm39)	I363F	possibly damaging	Het
Gm8267	A	T	14: 44,959,507 (GRCm39)	F186I	possibly damaging	Het
Hand1	A	G	11: 57,722,605 (GRCm39)	L3P	probably damaging	Het
Hmcn2	C	T	2: 31,315,189 (GRCm39)	L3671F	probably damaging	Het
Hspa12b	A	G	2: 130,982,922 (GRCm39)	D260G	probably benign	Het
Itih2	T	C	2: 10,111,619 (GRCm39)	K440E	probably damaging	Het
Morn4	A	T	19: 42,066,439 (GRCm39)	N49K	possibly damaging	Het
Mtmr6	T	G	14: 60,527,128 (GRCm39)		probably null	Het
Mylk	A	T	16: 34,817,176 (GRCm39)	D1848V	probably damaging	Het
Myo7b	A	G	18: 32,140,064 (GRCm39)	V242A	probably damaging	Het
Or4a74	T	C	2: 89,440,186 (GRCm39)	K87E	probably benign	Het
Or8g19	T	G	9: 39,055,386 (GRCm39)		probably benign	Het
Rmdn3	T	A	2: 118,969,846 (GRCm39)	Q405L	possibly damaging	Het
Robo2	A	G	16: 73,703,798 (GRCm39)	S30P		Het
Suclg1	G	T	6: 73,246,362 (GRCm39)	L218F	probably damaging	Het
Tdrd9	T	C	12: 112,002,889 (GRCm39)	L846P	probably damaging	Het
Tgfbi	A	G	13: 56,773,599 (GRCm39)	N199S	probably benign	Het
Traj35	C	T	14: 54,421,272 (GRCm39)	T14I		Het
Tril	A	G	6: 53,797,183 (GRCm39)	V13A	probably benign	Het
Tshz2	T	A	2: 169,727,278 (GRCm39)	S625T	probably benign	Het
Ttll12	A	G	15: 83,464,784 (GRCm39)	F464L	probably damaging	Het
Wdr35	T	C	12: 9,075,044 (GRCm39)		probably null	Het
Zfp346	A	G	13: 55,261,491 (GRCm39)	S94G	possibly damaging	Het
Zfp429	T	A	13: 67,538,331 (GRCm39)	Y371F	probably damaging	Het
Zscan4-ps2	A	T	7: 11,251,522 (GRCm39)	E186V	probably benign	Het

Other mutations in Tas2r134

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00562:Tas2r134	APN	2	51,518,100 (GRCm39)	missense	possibly damaging	0.90
IGL00563:Tas2r134	APN	2	51,518,100 (GRCm39)	missense	possibly damaging	0.90
IGL01121:Tas2r134	APN	2	51,518,001 (GRCm39)	missense	probably damaging	1.00
IGL01122:Tas2r134	APN	2	51,517,671 (GRCm39)	missense	probably damaging	1.00
IGL01128:Tas2r134	APN	2	51,517,671 (GRCm39)	missense	probably damaging	1.00
IGL01132:Tas2r134	APN	2	51,517,671 (GRCm39)	missense	probably damaging	1.00
IGL01312:Tas2r134	APN	2	51,518,247 (GRCm39)	missense	probably damaging	1.00
IGL01419:Tas2r134	APN	2	51,517,759 (GRCm39)	nonsense	probably null
IGL01774:Tas2r134	APN	2	51,518,160 (GRCm39)	missense	probably benign	0.07
IGL02735:Tas2r134	APN	2	51,517,839 (GRCm39)	missense	probably damaging	1.00
R0799:Tas2r134	UTSW	2	51,518,385 (GRCm39)	missense	probably benign	0.00
R1205:Tas2r134	UTSW	2	51,517,998 (GRCm39)	missense	probably benign	0.00
R2267:Tas2r134	UTSW	2	51,518,249 (GRCm39)	missense	probably benign	0.01
R3736:Tas2r134	UTSW	2	51,517,786 (GRCm39)	missense	probably damaging	1.00
R5254:Tas2r134	UTSW	2	51,517,559 (GRCm39)	missense	probably benign	0.39
R5752:Tas2r134	UTSW	2	51,517,880 (GRCm39)	missense	probably damaging	1.00
R6162:Tas2r134	UTSW	2	51,517,571 (GRCm39)	missense	probably damaging	1.00
R6940:Tas2r134	UTSW	2	51,518,148 (GRCm39)	missense	probably benign	0.00
R6954:Tas2r134	UTSW	2	51,517,782 (GRCm39)	missense	probably benign	0.00
R6996:Tas2r134	UTSW	2	51,517,601 (GRCm39)	missense	probably benign	0.00
R7206:Tas2r134	UTSW	2	51,518,120 (GRCm39)	missense	probably benign	0.02
R7303:Tas2r134	UTSW	2	51,518,145 (GRCm39)	missense	probably benign	0.01
R7348:Tas2r134	UTSW	2	51,518,414 (GRCm39)	missense	possibly damaging	0.92
R7479:Tas2r134	UTSW	2	51,517,541 (GRCm39)	missense	not run
R7575:Tas2r134	UTSW	2	51,518,166 (GRCm39)	missense	probably damaging	1.00
R7686:Tas2r134	UTSW	2	51,518,255 (GRCm39)	missense	possibly damaging	0.54
R9384:Tas2r134	UTSW	2	51,518,034 (GRCm39)	missense	probably damaging	0.97
R9622:Tas2r134	UTSW	2	51,518,358 (GRCm39)	missense	possibly damaging	0.89

Predicted Primers

PCR Primer
(F):5'- GCATGTATCCAGGAAGAGGATTC -3'
(R):5'- TGTTGGTGAAGTCCCAGAGG -3'

Sequencing Primer
(F):5'- GATTCAGCAAGGTGATTGGC -3'
(R):5'- CTTTATGGTCCAAAACATAGCAGAGG -3'

Posted On

2021-03-08