Mutagenetix > Incidental Mutation

Incidental Mutation 'R8735:Tenm4'

662972

Institutional Source

Beutler Lab

Gene Symbol

Tenm4

Ensembl Gene

ENSMUSG00000048078

Gene Name

teneurin transmembrane protein 4

Synonyms

Doc4, l7Rn3, Ten-m4, ELM2, l(7)-3Rn, Odz4

MMRRC Submission

068583-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8735 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

96171246-96911093 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 96905941 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 2618 (P2618S)

Ref Sequence

ENSEMBL: ENSMUSP00000102783 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000107162] [ENSMUST00000107165] [ENSMUST00000107166]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000107162
AA Change: P2610S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000102780
Gene: ENSMUSG00000048078
AA Change: P2610S

Domain	Start	End	E-Value	Type
Pfam:Ten_N	10	410	5.6e-195	PFAM
transmembrane domain	411	433	N/A	INTRINSIC
EGF_like	637	665	3.43e1	SMART
EGF	668	696	2.29e1	SMART
EGF	701	730	1.88e-1	SMART
EGF	733	762	1.13e1	SMART
EGF	767	797	2.39e1	SMART
EGF	800	828	4.32e-1	SMART
EGF	831	859	6.02e0	SMART
EGF	862	894	9.93e-1	SMART
low complexity region	900	914	N/A	INTRINSIC
Pfam:RHS_repeat	2327	2380	5.5e-7	PFAM
Pfam:Tox-GHH	2740	2818	5.2e-34	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000107165
AA Change: P2618S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000102783
Gene: ENSMUSG00000048078
AA Change: P2618S

Domain	Start	End	E-Value	Type
Pfam:Ten_N	36	402	1.1e-171	PFAM
transmembrane domain	403	425	N/A	INTRINSIC
EGF_like	629	657	3.43e1	SMART
EGF	660	688	2.29e1	SMART
EGF	693	722	1.88e-1	SMART
EGF	725	754	1.13e1	SMART
EGF	759	789	2.39e1	SMART
EGF	792	820	4.32e-1	SMART
EGF	823	851	6.02e0	SMART
EGF	863	895	9.93e-1	SMART
low complexity region	901	915	N/A	INTRINSIC
Pfam:RHS_repeat	2335	2368	1.6e-7	PFAM
Pfam:Tox-GHH	2749	2826	1.8e-32	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000107166
AA Change: P2581S

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000102784
Gene: ENSMUSG00000048078
AA Change: P2581S

Domain	Start	End	E-Value	Type
Pfam:Ten_N	35	193	1.4e-83	PFAM
Pfam:Ten_N	187	365	5e-78	PFAM
transmembrane domain	366	388	N/A	INTRINSIC
EGF_like	592	620	3.43e1	SMART
EGF	623	651	2.29e1	SMART
EGF	656	685	1.88e-1	SMART
EGF	688	717	1.13e1	SMART
EGF	722	752	2.39e1	SMART
EGF	755	783	4.32e-1	SMART
EGF	786	814	6.02e0	SMART
EGF	826	858	9.93e-1	SMART
low complexity region	864	878	N/A	INTRINSIC
Pfam:RHS_repeat	2298	2351	3.8e-7	PFAM
Pfam:Tox-GHH	2711	2789	3.9e-34	PFAM

Meta Mutation Damage Score

0.0602

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

99% (76/77)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene plays a role in establishing proper neuronal connectivity during development. Defects in this gene have been associated with hereditary essential tremor-5. [provided by RefSeq, Oct 2016]
PHENOTYPE: Various ENU-induced alleles cause prenatal lethality associated with impaired mesoderm development and lead to pleiotropic phenotypes. The most severe alleles cause failure of gastrulation and somitogenesis while the least severe one allows survival to adulthood with runting of variable penetrance. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310007B03Rik	C	T	1: 93,154,486 (GRCm38)		probably null	Het
6430550D23Rik	A	G	2: 156,003,811 (GRCm38)	F9S	unknown	Het
Abhd10	A	G	16: 45,737,634 (GRCm38)	F101L	probably damaging	Het
Acp2	A	G	2: 91,204,306 (GRCm38)	T93A	probably benign	Het
Adamts9	C	T	6: 92,860,067 (GRCm38)		probably benign	Het
Aknad1	T	A	3: 108,775,299 (GRCm38)		probably benign	Het
Ank3	A	T	10: 69,986,955 (GRCm38)	T485S	probably benign	Het
Caly	G	A	7: 140,072,590 (GRCm38)	R82C	probably damaging	Het
Cfhr2	A	G	1: 139,858,605 (GRCm38)	L8P	probably damaging	Het
Cpeb2	T	A	5: 43,281,432 (GRCm38)	L869*	probably null	Het
Cxxc1	A	G	18: 74,217,260 (GRCm38)	K33E	possibly damaging	Het
Cyp17a1	T	C	19: 46,671,094 (GRCm38)		probably null	Het
Cyp4a30b	G	A	4: 115,452,779 (GRCm38)	W59*	probably null	Het
Dennd4b	T	C	3: 90,277,865 (GRCm38)	V1204A	probably damaging	Het
Dpyd	T	A	3: 119,141,916 (GRCm38)	D663E	possibly damaging	Het
Esrrg	A	T	1: 188,201,008 (GRCm38)		probably benign	Het
Exoc2	A	C	13: 30,906,839 (GRCm38)	L261V	probably damaging	Het
Exosc9	T	C	3: 36,555,513 (GRCm38)	L185S	probably damaging	Het
Gcnt3	T	C	9: 70,034,446 (GRCm38)	K280R	probably benign	Het
Gm35339	T	C	15: 76,356,575 (GRCm38)	Y488H		Het
Ighv1-64	A	T	12: 115,507,597 (GRCm38)	M100K	probably benign	Het
Impdh2	G	A	9: 108,564,779 (GRCm38)		probably null	Het
Itsn2	T	A	12: 4,671,474 (GRCm38)	I1068K	probably damaging	Het
Kif5c	T	C	2: 49,694,771 (GRCm38)	C169R	probably damaging	Het
Kndc1	A	G	7: 139,910,214 (GRCm38)	S211G	probably benign	Het
Lama2	T	A	10: 27,190,534 (GRCm38)	E1117V	probably damaging	Het
Lgals4	C	T	7: 28,841,496 (GRCm38)	R282C	probably damaging	Het
Lsg1	T	A	16: 30,581,047 (GRCm38)		probably null	Het
Msh3	A	G	13: 92,274,866 (GRCm38)	S608P	possibly damaging	Het
Mslnl	T	A	17: 25,745,088 (GRCm38)	F464I	probably benign	Het
Mycbp2	T	A	14: 103,223,150 (GRCm38)	K1493N	probably damaging	Het
Myo15	A	T	11: 60,510,853 (GRCm38)		probably null	Het
Myo1b	A	T	1: 51,755,737 (GRCm38)	F1065I	probably benign	Het
Nf1	A	G	11: 79,454,310 (GRCm38)		probably benign	Het
Nup153	C	A	13: 46,727,551 (GRCm38)		probably benign	Het
Olfr1291-ps1	T	A	2: 111,500,152 (GRCm38)	I300K	probably damaging	Het
Olfr1447	A	G	19: 12,900,910 (GRCm38)	V290A	possibly damaging	Het
Osbpl7	A	G	11: 97,052,368 (GRCm38)	T149A	probably benign	Het
Parp1	A	G	1: 180,569,125 (GRCm38)	D31G	probably benign	Het
Pcdha6	T	A	18: 36,968,150 (GRCm38)	V132E	possibly damaging	Het
Pcdhb8	T	A	18: 37,356,922 (GRCm38)	L551Q	possibly damaging	Het
Pcsk9	T	A	4: 106,454,611 (GRCm38)	T190S	probably damaging	Het
Pdgfrb	A	T	18: 61,063,977 (GRCm38)	T162S	probably benign	Het
Pi4ka	C	T	16: 17,318,370 (GRCm38)	R908Q		Het
Pnpla8	A	G	12: 44,283,439 (GRCm38)	E258G	probably benign	Het
Prrc2c	A	T	1: 162,709,558 (GRCm38)	S643T	unknown	Het
Rnf20	T	G	4: 49,655,964 (GRCm38)	I970S	possibly damaging	Het
Robo2	A	T	16: 73,958,359 (GRCm38)	V758D	probably damaging	Het
Robo4	G	A	9: 37,408,281 (GRCm38)	S609N	possibly damaging	Het
Rrbp1	G	A	2: 143,989,000 (GRCm38)	Q416*	probably null	Het
Rsf1	GCG	GCGACGGCGACG	7: 97,579,907 (GRCm38)		probably benign	Het
Ryr2	C	A	13: 11,686,947 (GRCm38)	E2941D	probably damaging	Het
Scd2	G	T	19: 44,301,304 (GRCm38)	C246F	probably benign	Het
Sh3rf1	G	A	8: 61,372,653 (GRCm38)	V561I	probably benign	Het
Slc4a4	T	A	5: 89,132,442 (GRCm38)	D366E	probably damaging	Het
Slfn14	T	G	11: 83,283,889 (GRCm38)	Q92P	probably damaging	Het
Slfn4	A	T	11: 83,186,944 (GRCm38)	Y186F	probably damaging	Het
Smpd4	T	C	16: 17,635,546 (GRCm38)	L309P	possibly damaging	Het
Smyd3	G	T	1: 179,092,917 (GRCm38)	N217K	probably benign	Het
Spen	T	C	4: 141,469,818 (GRCm38)	T3547A	probably benign	Het
Suclg1	A	T	6: 73,276,746 (GRCm38)	I126F	unknown	Het
Tmprss15	A	T	16: 79,001,814 (GRCm38)	I660N	possibly damaging	Het
Trf	A	T	9: 103,210,524 (GRCm38)	V677D	probably damaging	Het
Trim36	T	C	18: 46,169,385 (GRCm38)	N532S	probably benign	Het
Trim37	T	C	11: 87,147,059 (GRCm38)		probably null	Het
Ttn	T	C	2: 76,740,110 (GRCm38)	N26813S	probably benign	Het
Vmn1r199	T	A	13: 22,383,367 (GRCm38)	V277D	probably damaging	Het
Wdfy3	T	C	5: 101,930,085 (GRCm38)	D873G	probably benign	Het
Wdr27	T	C	17: 14,883,667 (GRCm38)	T726A	probably damaging	Het
Wdtc1	A	T	4: 133,304,200 (GRCm38)	C236*	probably null	Het
Wnk4	A	C	11: 101,276,266 (GRCm38)	S1083R	unknown	Het
Wwc1	A	T	11: 35,883,407 (GRCm38)	I342N	probably damaging	Het
Zbtb40	A	G	4: 136,998,646 (GRCm38)	L534P	probably damaging	Het
Zfp62	T	C	11: 49,217,400 (GRCm38)	C773R	probably damaging	Het
Zfp709	T	A	8: 71,889,183 (GRCm38)	I152N	probably benign	Het
Zfp738	T	C	13: 67,671,431 (GRCm38)	Y147C	probably damaging	Het
Zscan18	A	C	7: 12,769,698 (GRCm38)	S645A	probably benign	Het
Zw10	A	G	9: 49,077,561 (GRCm38)	Y709C	probably damaging	Het

Other mutations in Tenm4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00228:Tenm4	APN	7	96,868,009 (GRCm38)	missense	probably benign	0.00
IGL00468:Tenm4	APN	7	96,874,472 (GRCm38)	missense	probably damaging	0.98
IGL00519:Tenm4	APN	7	96,805,138 (GRCm38)	splice site	probably benign
IGL00979:Tenm4	APN	7	96,729,391 (GRCm38)	missense	probably damaging	0.96
IGL01401:Tenm4	APN	7	96,874,267 (GRCm38)	missense	probably damaging	1.00
IGL01459:Tenm4	APN	7	96,729,385 (GRCm38)	missense	probably damaging	1.00
IGL01519:Tenm4	APN	7	96,895,177 (GRCm38)	missense	probably damaging	1.00
IGL01545:Tenm4	APN	7	96,874,303 (GRCm38)	missense	probably benign	0.00
IGL01579:Tenm4	APN	7	96,863,502 (GRCm38)	missense	probably benign	0.00
IGL01587:Tenm4	APN	7	96,863,502 (GRCm38)	missense	probably benign	0.00
IGL01625:Tenm4	APN	7	96,885,358 (GRCm38)	missense	probably damaging	1.00
IGL01655:Tenm4	APN	7	96,553,724 (GRCm38)	missense	probably damaging	1.00
IGL01683:Tenm4	APN	7	96,885,404 (GRCm38)	missense	possibly damaging	0.84
IGL01728:Tenm4	APN	7	96,896,064 (GRCm38)	missense	probably damaging	1.00
IGL01732:Tenm4	APN	7	96,895,509 (GRCm38)	missense	probably damaging	1.00
IGL01924:Tenm4	APN	7	96,895,212 (GRCm38)	missense	probably damaging	1.00
IGL01966:Tenm4	APN	7	96,553,550 (GRCm38)	missense	probably damaging	1.00
IGL02177:Tenm4	APN	7	96,895,662 (GRCm38)	missense	probably benign	0.40
IGL02207:Tenm4	APN	7	96,874,116 (GRCm38)	missense	possibly damaging	0.85
IGL02269:Tenm4	APN	7	96,823,822 (GRCm38)	missense	probably damaging	1.00
IGL02274:Tenm4	APN	7	96,854,734 (GRCm38)	missense	probably damaging	1.00
IGL02375:Tenm4	APN	7	96,704,137 (GRCm38)	missense	possibly damaging	0.52
IGL02415:Tenm4	APN	7	96,874,074 (GRCm38)	missense	probably damaging	0.98
IGL02472:Tenm4	APN	7	96,774,176 (GRCm38)	unclassified	probably benign
IGL02656:Tenm4	APN	7	96,885,433 (GRCm38)	missense	probably damaging	1.00
IGL02678:Tenm4	APN	7	96,896,219 (GRCm38)	missense	probably damaging	1.00
IGL02829:Tenm4	APN	7	96,894,998 (GRCm38)	nonsense	probably null
IGL02863:Tenm4	APN	7	96,873,706 (GRCm38)	missense	probably damaging	1.00
IGL03145:Tenm4	APN	7	96,842,968 (GRCm38)	missense	probably damaging	0.98
IGL03153:Tenm4	APN	7	96,873,762 (GRCm38)	missense	probably damaging	1.00
principium	UTSW	7	96,797,481 (GRCm38)	missense	probably damaging	0.98
toccata	UTSW	7	96,902,989 (GRCm38)	critical splice donor site	probably null
P0026:Tenm4	UTSW	7	96,874,527 (GRCm38)	missense	probably damaging	1.00
R0097:Tenm4	UTSW	7	96,892,926 (GRCm38)	missense	probably damaging	1.00
R0097:Tenm4	UTSW	7	96,892,926 (GRCm38)	missense	probably damaging	1.00
R0140:Tenm4	UTSW	7	96,896,052 (GRCm38)	missense	possibly damaging	0.78
R0164:Tenm4	UTSW	7	96,729,340 (GRCm38)	splice site	probably benign
R0277:Tenm4	UTSW	7	96,694,950 (GRCm38)	missense	possibly damaging	0.54
R0323:Tenm4	UTSW	7	96,694,950 (GRCm38)	missense	possibly damaging	0.54
R0362:Tenm4	UTSW	7	96,772,035 (GRCm38)	nonsense	probably null
R0381:Tenm4	UTSW	7	96,905,881 (GRCm38)	missense	probably damaging	1.00
R0420:Tenm4	UTSW	7	96,873,766 (GRCm38)	missense	possibly damaging	0.85
R0426:Tenm4	UTSW	7	96,777,851 (GRCm38)	missense	probably damaging	1.00
R0513:Tenm4	UTSW	7	96,895,623 (GRCm38)	missense	probably benign	0.35
R0624:Tenm4	UTSW	7	96,774,020 (GRCm38)	missense	probably damaging	1.00
R0837:Tenm4	UTSW	7	96,896,275 (GRCm38)	splice site	probably benign
R1037:Tenm4	UTSW	7	96,797,481 (GRCm38)	missense	probably damaging	0.98
R1172:Tenm4	UTSW	7	96,848,044 (GRCm38)	missense	probably damaging	1.00
R1422:Tenm4	UTSW	7	96,550,051 (GRCm38)	missense	probably damaging	0.99
R1427:Tenm4	UTSW	7	96,843,048 (GRCm38)	missense	probably benign	0.42
R1462:Tenm4	UTSW	7	96,704,153 (GRCm38)	missense	probably damaging	1.00
R1462:Tenm4	UTSW	7	96,704,153 (GRCm38)	missense	probably damaging	1.00
R1597:Tenm4	UTSW	7	96,902,989 (GRCm38)	critical splice donor site	probably null
R1701:Tenm4	UTSW	7	96,902,889 (GRCm38)	missense	probably damaging	1.00
R1707:Tenm4	UTSW	7	96,888,685 (GRCm38)	missense	probably damaging	1.00
R1809:Tenm4	UTSW	7	96,873,780 (GRCm38)	missense	probably benign	0.17
R1812:Tenm4	UTSW	7	96,895,940 (GRCm38)	missense	probably damaging	1.00
R1895:Tenm4	UTSW	7	96,735,808 (GRCm38)	missense	probably damaging	1.00
R1933:Tenm4	UTSW	7	96,895,326 (GRCm38)	missense	probably damaging	1.00
R1946:Tenm4	UTSW	7	96,735,808 (GRCm38)	missense	probably damaging	1.00
R2108:Tenm4	UTSW	7	96,906,290 (GRCm38)	missense	probably damaging	1.00
R2151:Tenm4	UTSW	7	96,902,847 (GRCm38)	missense	probably damaging	1.00
R2247:Tenm4	UTSW	7	96,906,009 (GRCm38)	missense	probably benign	0.03
R2329:Tenm4	UTSW	7	96,895,862 (GRCm38)	missense	probably benign	0.00
R2893:Tenm4	UTSW	7	96,894,990 (GRCm38)	missense	probably damaging	1.00
R2990:Tenm4	UTSW	7	96,893,125 (GRCm38)	splice site	probably null
R3409:Tenm4	UTSW	7	96,895,160 (GRCm38)	missense	probably damaging	1.00
R3410:Tenm4	UTSW	7	96,852,530 (GRCm38)	missense	probably damaging	0.99
R3411:Tenm4	UTSW	7	96,852,530 (GRCm38)	missense	probably damaging	0.99
R3440:Tenm4	UTSW	7	96,553,516 (GRCm38)	missense	probably benign	0.00
R3441:Tenm4	UTSW	7	96,553,516 (GRCm38)	missense	probably benign	0.00
R3719:Tenm4	UTSW	7	96,863,563 (GRCm38)	missense	possibly damaging	0.92
R3772:Tenm4	UTSW	7	96,694,880 (GRCm38)	missense	probably damaging	1.00
R3773:Tenm4	UTSW	7	96,694,880 (GRCm38)	missense	probably damaging	1.00
R4093:Tenm4	UTSW	7	96,895,772 (GRCm38)	missense	probably damaging	1.00
R4439:Tenm4	UTSW	7	96,895,815 (GRCm38)	missense	probably benign	0.01
R4441:Tenm4	UTSW	7	96,895,815 (GRCm38)	missense	probably benign	0.01
R4510:Tenm4	UTSW	7	96,894,863 (GRCm38)	missense	probably benign
R4511:Tenm4	UTSW	7	96,894,863 (GRCm38)	missense	probably benign
R4543:Tenm4	UTSW	7	96,895,815 (GRCm38)	missense	probably benign	0.01
R4645:Tenm4	UTSW	7	96,895,742 (GRCm38)	missense	probably damaging	1.00
R4701:Tenm4	UTSW	7	96,895,349 (GRCm38)	missense	probably damaging	1.00
R4707:Tenm4	UTSW	7	96,774,046 (GRCm38)	missense	probably damaging	0.99
R4714:Tenm4	UTSW	7	96,894,924 (GRCm38)	missense	probably damaging	1.00
R4742:Tenm4	UTSW	7	96,797,484 (GRCm38)	missense	probably damaging	0.99
R4784:Tenm4	UTSW	7	96,774,046 (GRCm38)	missense	probably damaging	0.99
R4785:Tenm4	UTSW	7	96,774,046 (GRCm38)	missense	probably damaging	0.99
R4801:Tenm4	UTSW	7	96,906,245 (GRCm38)	missense	probably damaging	0.97
R4802:Tenm4	UTSW	7	96,906,245 (GRCm38)	missense	probably damaging	0.97
R4880:Tenm4	UTSW	7	96,905,818 (GRCm38)	splice site	probably null
R5036:Tenm4	UTSW	7	96,852,561 (GRCm38)	missense	probably damaging	1.00
R5036:Tenm4	UTSW	7	96,694,790 (GRCm38)	missense	probably damaging	1.00
R5050:Tenm4	UTSW	7	96,895,788 (GRCm38)	missense	probably damaging	1.00
R5103:Tenm4	UTSW	7	96,842,957 (GRCm38)	missense	probably damaging	1.00
R5106:Tenm4	UTSW	7	96,843,149 (GRCm38)	missense	probably damaging	0.99
R5118:Tenm4	UTSW	7	96,893,086 (GRCm38)	missense	probably damaging	1.00
R5272:Tenm4	UTSW	7	96,874,203 (GRCm38)	missense	probably damaging	0.98
R5282:Tenm4	UTSW	7	96,837,331 (GRCm38)	missense	possibly damaging	0.90
R5403:Tenm4	UTSW	7	96,888,827 (GRCm38)	missense	probably damaging	1.00
R5404:Tenm4	UTSW	7	96,894,680 (GRCm38)	missense	probably damaging	1.00
R5567:Tenm4	UTSW	7	96,896,209 (GRCm38)	nonsense	probably null
R5590:Tenm4	UTSW	7	96,797,401 (GRCm38)	missense	possibly damaging	0.93
R5590:Tenm4	UTSW	7	96,797,400 (GRCm38)	missense	possibly damaging	0.73
R5597:Tenm4	UTSW	7	96,553,517 (GRCm38)	missense	probably benign	0.00
R5782:Tenm4	UTSW	7	96,893,039 (GRCm38)	missense	probably benign	0.00
R5861:Tenm4	UTSW	7	96,843,217 (GRCm38)	intron	probably benign
R5890:Tenm4	UTSW	7	96,902,860 (GRCm38)	missense	probably damaging	1.00
R5930:Tenm4	UTSW	7	96,854,719 (GRCm38)	missense	probably damaging	1.00
R5940:Tenm4	UTSW	7	96,845,895 (GRCm38)	missense	probably damaging	1.00
R6012:Tenm4	UTSW	7	96,522,433 (GRCm38)	intron	probably benign
R6060:Tenm4	UTSW	7	96,873,711 (GRCm38)	missense	probably damaging	1.00
R6104:Tenm4	UTSW	7	96,837,289 (GRCm38)	missense	probably damaging	0.97
R6283:Tenm4	UTSW	7	96,874,494 (GRCm38)	missense	probably benign	0.33
R6333:Tenm4	UTSW	7	96,774,124 (GRCm38)	missense	probably damaging	1.00
R6522:Tenm4	UTSW	7	96,843,044 (GRCm38)	missense	possibly damaging	0.88
R6616:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R6746:Tenm4	UTSW	7	96,892,860 (GRCm38)	missense	probably damaging	1.00
R6751:Tenm4	UTSW	7	96,845,712 (GRCm38)	missense	possibly damaging	0.95
R6806:Tenm4	UTSW	7	96,811,959 (GRCm38)	missense	possibly damaging	0.95
R6807:Tenm4	UTSW	7	96,895,271 (GRCm38)	missense	probably damaging	1.00
R6807:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R6809:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R6810:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R6811:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R6853:Tenm4	UTSW	7	96,837,295 (GRCm38)	missense	possibly damaging	0.94
R6886:Tenm4	UTSW	7	96,797,392 (GRCm38)	missense	possibly damaging	0.85
R6920:Tenm4	UTSW	7	96,895,550 (GRCm38)	missense	probably damaging	1.00
R6937:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R6939:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R7011:Tenm4	UTSW	7	96,896,135 (GRCm38)	nonsense	probably null
R7033:Tenm4	UTSW	7	96,895,223 (GRCm38)	nonsense	probably null
R7040:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R7083:Tenm4	UTSW	7	96,895,349 (GRCm38)	missense	probably damaging	1.00
R7238:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R7239:Tenm4	UTSW	7	96,735,813 (GRCm38)	missense	possibly damaging	0.47
R7239:Tenm4	UTSW	7	96,553,496 (GRCm38)	missense	probably benign	0.01
R7337:Tenm4	UTSW	7	96,874,126 (GRCm38)	missense	probably benign	0.44
R7400:Tenm4	UTSW	7	96,694,803 (GRCm38)	missense	probably damaging	0.97
R7407:Tenm4	UTSW	7	96,773,987 (GRCm38)	missense	possibly damaging	0.89
R7449:Tenm4	UTSW	7	96,874,213 (GRCm38)	missense	possibly damaging	0.65
R7473:Tenm4	UTSW	7	96,774,146 (GRCm38)	missense	probably damaging	1.00
R7477:Tenm4	UTSW	7	96,845,808 (GRCm38)	missense	probably damaging	0.99
R7489:Tenm4	UTSW	7	96,837,314 (GRCm38)	missense	possibly damaging	0.90
R7498:Tenm4	UTSW	7	96,848,017 (GRCm38)	missense	probably damaging	1.00
R7562:Tenm4	UTSW	7	96,888,814 (GRCm38)	missense	probably damaging	1.00
R7615:Tenm4	UTSW	7	96,845,926 (GRCm38)	missense	probably damaging	1.00
R7624:Tenm4	UTSW	7	96,895,985 (GRCm38)	missense	possibly damaging	0.95
R7626:Tenm4	UTSW	7	96,893,014 (GRCm38)	missense	probably damaging	1.00
R7690:Tenm4	UTSW	7	96,863,533 (GRCm38)	missense	probably benign	0.00
R7692:Tenm4	UTSW	7	96,895,403 (GRCm38)	missense	probably damaging	1.00
R7748:Tenm4	UTSW	7	96,894,702 (GRCm38)	missense	probably damaging	1.00
R7763:Tenm4	UTSW	7	96,895,692 (GRCm38)	missense	probably benign	0.38
R7792:Tenm4	UTSW	7	96,774,014 (GRCm38)	missense	possibly damaging	0.54
R7855:Tenm4	UTSW	7	96,873,874 (GRCm38)	missense	probably damaging	1.00
R7868:Tenm4	UTSW	7	96,906,380 (GRCm38)	missense	possibly damaging	0.79
R7878:Tenm4	UTSW	7	96,852,357 (GRCm38)	missense	probably damaging	1.00
R7997:Tenm4	UTSW	7	96,874,305 (GRCm38)	missense	probably benign	0.44
R8017:Tenm4	UTSW	7	96,704,041 (GRCm38)	missense	probably damaging	1.00
R8019:Tenm4	UTSW	7	96,704,041 (GRCm38)	missense	probably damaging	1.00
R8054:Tenm4	UTSW	7	96,729,346 (GRCm38)	splice site	probably benign
R8061:Tenm4	UTSW	7	96,852,456 (GRCm38)	missense	probably damaging	1.00
R8108:Tenm4	UTSW	7	96,854,728 (GRCm38)	missense	probably benign	0.39
R8140:Tenm4	UTSW	7	96,895,176 (GRCm38)	missense	probably damaging	1.00
R8214:Tenm4	UTSW	7	96,895,407 (GRCm38)	missense	probably damaging	1.00
R8258:Tenm4	UTSW	7	96,867,991 (GRCm38)	missense	probably damaging	1.00
R8259:Tenm4	UTSW	7	96,867,991 (GRCm38)	missense	probably damaging	1.00
R8364:Tenm4	UTSW	7	96,772,106 (GRCm38)	critical splice donor site	probably null
R8542:Tenm4	UTSW	7	96,811,932 (GRCm38)	missense	probably damaging	0.99
R8669:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8670:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8683:Tenm4	UTSW	7	96,902,857 (GRCm38)	missense	probably damaging	0.99
R8691:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8692:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8714:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8716:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8736:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8737:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8738:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8738:Tenm4	UTSW	7	96,873,840 (GRCm38)	missense	probably damaging	1.00
R8739:Tenm4	UTSW	7	96,905,941 (GRCm38)	missense	probably benign
R8776:Tenm4	UTSW	7	96,895,032 (GRCm38)	missense	probably damaging	1.00
R8776-TAIL:Tenm4	UTSW	7	96,895,032 (GRCm38)	missense	probably damaging	1.00
R8777:Tenm4	UTSW	7	96,896,037 (GRCm38)	missense	probably damaging	1.00
R8777-TAIL:Tenm4	UTSW	7	96,896,037 (GRCm38)	missense	probably damaging	1.00
R8817:Tenm4	UTSW	7	96,874,128 (GRCm38)	missense	probably benign	0.01
R8851:Tenm4	UTSW	7	96,852,503 (GRCm38)	missense	probably damaging	1.00
R8913:Tenm4	UTSW	7	96,702,745 (GRCm38)	splice site	probably benign
R8977:Tenm4	UTSW	7	96,811,970 (GRCm38)	missense	probably damaging	1.00
R9100:Tenm4	UTSW	7	96,845,854 (GRCm38)	missense	probably damaging	1.00
R9136:Tenm4	UTSW	7	96,823,918 (GRCm38)	missense	possibly damaging	0.69
R9163:Tenm4	UTSW	7	96,823,873 (GRCm38)	missense	probably damaging	1.00
R9188:Tenm4	UTSW	7	96,772,027 (GRCm38)	missense	probably damaging	1.00
R9195:Tenm4	UTSW	7	96,892,919 (GRCm38)	missense	probably damaging	1.00
R9217:Tenm4	UTSW	7	96,885,439 (GRCm38)	missense	probably damaging	1.00
R9344:Tenm4	UTSW	7	96,896,145 (GRCm38)	missense	probably damaging	1.00
R9414:Tenm4	UTSW	7	96,896,160 (GRCm38)	missense	probably benign
R9466:Tenm4	UTSW	7	96,550,045 (GRCm38)	missense	possibly damaging	0.79
R9559:Tenm4	UTSW	7	96,823,849 (GRCm38)	missense	probably benign
R9626:Tenm4	UTSW	7	96,896,138 (GRCm38)	missense	probably damaging	1.00
R9673:Tenm4	UTSW	7	96,867,989 (GRCm38)	missense	probably damaging	1.00
R9676:Tenm4	UTSW	7	96,895,431 (GRCm38)	missense	probably damaging	1.00
R9678:Tenm4	UTSW	7	96,737,412 (GRCm38)	missense	possibly damaging	0.94
R9775:Tenm4	UTSW	7	96,906,554 (GRCm38)	missense	possibly damaging	0.92
R9790:Tenm4	UTSW	7	96,888,839 (GRCm38)	missense	probably damaging	1.00
R9791:Tenm4	UTSW	7	96,888,839 (GRCm38)	missense	probably damaging	1.00
R9803:Tenm4	UTSW	7	96,553,478 (GRCm38)	missense	probably damaging	1.00
X0021:Tenm4	UTSW	7	96,873,909 (GRCm38)	nonsense	probably null
X0026:Tenm4	UTSW	7	96,868,087 (GRCm38)	missense	probably damaging	0.98
X0066:Tenm4	UTSW	7	96,894,794 (GRCm38)	missense	probably damaging	1.00
X0066:Tenm4	UTSW	7	96,848,030 (GRCm38)	missense	probably damaging	1.00
Z1176:Tenm4	UTSW	7	96,905,914 (GRCm38)	missense	probably benign	0.00
Z1177:Tenm4	UTSW	7	96,863,585 (GRCm38)	missense	probably damaging	0.96

Predicted Primers

PCR Primer
(F):5'- ACTGATTCTCTGCACTCTTGGG -3'
(R):5'- AGGTCCTGGTTTCACAAAGTAGTG -3'

Sequencing Primer
(F):5'- GCACTCTTGGGGTTTGCCTC -3'
(R):5'- ACCCCATCAATGGTGAAGTG -3'

Posted On

2021-03-08