Mutagenetix > Incidental Mutation

Incidental Mutation 'R8737:Or10a3m'

663094

Institutional Source

Beutler Lab

Gene Symbol

Or10a3m

Ensembl Gene

ENSMUSG00000056946

Gene Name

olfactory receptor family 10 subfamily A member 3M

Synonyms

Olfr512, MOR268-3, GA_x6K02T2PBJ9-11043421-11044365

MMRRC Submission

068584-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.100) question?

Stock #

R8737 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

108312562-108313542 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 108312964 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 135 (F135I)

Ref Sequence

ENSEMBL: ENSMUSP00000074291 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074730] [ENSMUST00000209620]

AlphaFold

Q8VFZ4

Predicted Effect

probably damaging
Transcript: ENSMUST00000074730
AA Change: F135I

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000074291
Gene: ENSMUSG00000056946
AA Change: F135I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	43	320	7.8e-62	PFAM
Pfam:7TM_GPCR_Srsx	47	317	2.5e-6	PFAM
Pfam:7tm_1	53	302	2.3e-25	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000209620
AA Change: F123I

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

100% (67/67)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aff3	T	A	1: 38,308,810 (GRCm39)	D375V	probably damaging	Het
Agfg1	C	G	1: 82,871,243 (GRCm39)	P492A	probably benign	Het
Asb13	T	C	13: 3,692,089 (GRCm39)	V23A	probably damaging	Het
Atxn1l	A	T	8: 110,460,230 (GRCm39)	C11S	probably damaging	Het
Bbs9	T	G	9: 22,590,244 (GRCm39)	S661A	probably benign	Het
Btrc	T	C	19: 45,496,198 (GRCm39)	V211A	probably damaging	Het
Camk1g	T	C	1: 193,030,794 (GRCm39)		probably null	Het
Capn11	A	T	17: 45,943,801 (GRCm39)	L578I	probably benign	Het
Cd82	A	T	2: 93,252,239 (GRCm39)	I124N	probably damaging	Het
Cdkl4	G	T	17: 80,858,258 (GRCm39)	H120Q	probably benign	Het
Cftr	T	C	6: 18,319,728 (GRCm39)	V1393A	probably damaging	Het
Col14a1	A	T	15: 55,318,706 (GRCm39)	R1402*	probably null	Het
Col6a3	T	G	1: 90,727,747 (GRCm39)	Y1960S	probably benign	Het
Col9a1	A	T	1: 24,224,127 (GRCm39)	Y103F	unknown	Het
Cux1	A	G	5: 136,311,796 (GRCm39)	V1180A	probably damaging	Het
Cyp17a1	A	T	19: 46,658,166 (GRCm39)	C249S	probably benign	Het
Derl1	A	T	15: 57,755,568 (GRCm39)	W42R	probably benign	Het
Dhx36	C	A	3: 62,386,747 (GRCm39)	M668I	probably benign	Het
Dnah6	T	C	6: 73,044,428 (GRCm39)	K3228E	possibly damaging	Het
Dst	C	T	1: 34,267,750 (GRCm39)	A3050V	probably benign	Het
Efr3b	A	G	12: 4,049,594 (GRCm39)	Y70H	probably damaging	Het
Epb42	T	A	2: 120,856,324 (GRCm39)	T407S	possibly damaging	Het
Fads2b	T	A	2: 85,324,387 (GRCm39)		probably benign	Het
Fap	T	A	2: 62,342,777 (GRCm39)	I608L	probably benign	Het
Fblim1	A	G	4: 141,310,387 (GRCm39)	Y292H	probably benign	Het
Fmod	A	G	1: 133,968,043 (GRCm39)	I28V	probably benign	Het
Gad2	T	A	2: 22,524,985 (GRCm39)	Y256*	probably null	Het
Gapdh	G	A	6: 125,139,017 (GRCm39)	A353V	probably benign	Het
Gm5460	T	G	14: 33,739,149 (GRCm39)	W44G	unknown	Het
Ino80e	A	T	7: 126,460,974 (GRCm39)	V91D	probably benign	Het
Iqgap2	G	A	13: 95,802,258 (GRCm39)	P896S	probably damaging	Het
Kif26b	T	A	1: 178,692,430 (GRCm39)	I457N	probably damaging	Het
Klc2	T	A	19: 5,168,477 (GRCm39)		probably benign	Het
Ldlrap1	C	A	4: 134,495,147 (GRCm39)	W22L	probably benign	Het
Matn3	A	G	12: 9,017,665 (GRCm39)	D439G	possibly damaging	Het
Met	T	C	6: 17,540,510 (GRCm39)	L812P	probably benign	Het
Mki67	A	T	7: 135,315,504 (GRCm39)	I90N	probably damaging	Het
Mptx2	A	C	1: 173,105,256 (GRCm39)	S12A	probably benign	Het
Mrgpra9	G	T	7: 46,885,624 (GRCm39)	N14K	probably benign	Het
Nbeal2	G	A	9: 110,456,949 (GRCm39)	R2350C	probably damaging	Het
Nsmaf	A	G	4: 6,396,748 (GRCm39)	I913T	probably benign	Het
Nuggc	G	T	14: 65,882,535 (GRCm39)	C760F	probably benign	Het
Or1j13	T	A	2: 36,369,629 (GRCm39)	N171I	probably benign	Het
Or2y1f	T	A	11: 49,184,965 (GRCm39)	F272L	probably damaging	Het
Or4c110	C	A	2: 88,832,351 (GRCm39)	E94*	probably null	Het
Or51b6	A	G	7: 103,555,913 (GRCm39)	N86S		Het
Pdgfrb	C	T	18: 61,214,073 (GRCm39)	P953S	probably damaging	Het
Pemt	T	G	11: 59,874,285 (GRCm39)	I102L	probably benign	Het
Pla2g4c	G	T	7: 13,069,154 (GRCm39)	M109I	probably benign	Het
Pla2g4d	T	A	2: 120,100,466 (GRCm39)	Y622F	probably damaging	Het
Pmpca	T	A	2: 26,283,531 (GRCm39)	V400D	probably damaging	Het
Pnpt1	G	C	11: 29,104,815 (GRCm39)		probably null	Het
Pramel29	A	C	4: 143,935,192 (GRCm39)	L183R	probably damaging	Het
Prrc1	T	A	18: 57,496,408 (GRCm39)	S120T	possibly damaging	Het
Prrx2	T	G	2: 30,768,578 (GRCm39)	V135G	probably damaging	Het
Qrfprl	A	G	6: 65,433,260 (GRCm39)	K360R	probably benign	Het
Recql4	T	C	15: 76,593,054 (GRCm39)	N352S	probably benign	Het
Sftpa1	T	C	14: 40,856,044 (GRCm39)	S130P	probably damaging	Het
Slc35e2	T	C	4: 155,695,042 (GRCm39)	L136P	probably benign	Het
Tarbp2	T	A	15: 102,430,202 (GRCm39)	S155T	probably benign	Het
Tenm4	C	T	7: 96,555,148 (GRCm39)	P2618S	probably benign	Het
Tmem116	A	T	5: 121,620,433 (GRCm39)	Y146F		Het
Ttc17	C	T	2: 94,206,374 (GRCm39)		probably null	Het
Vmn2r114	G	A	17: 23,529,142 (GRCm39)	T320I	probably benign	Het
Vmn2r69	A	T	7: 85,055,783 (GRCm39)	V785D	probably damaging	Het
Vmn2r89	T	A	14: 51,693,722 (GRCm39)	N357K	probably damaging	Het
Zfp811	A	T	17: 33,017,197 (GRCm39)	L281Q	possibly damaging	Het

Other mutations in Or10a3m

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01889:Or10a3m	APN	7	108,313,089 (GRCm39)	missense	probably benign	0.02
IGL01912:Or10a3m	APN	7	108,313,465 (GRCm39)	missense	possibly damaging	0.60
IGL02182:Or10a3m	APN	7	108,313,075 (GRCm39)	missense	probably benign	0.02
IGL02409:Or10a3m	APN	7	108,313,366 (GRCm39)	missense	probably benign	0.00
IGL02554:Or10a3m	APN	7	108,312,949 (GRCm39)	missense	possibly damaging	0.94
IGL03210:Or10a3m	APN	7	108,312,775 (GRCm39)	missense	probably damaging	1.00
IGL03373:Or10a3m	APN	7	108,313,339 (GRCm39)	missense	probably damaging	1.00
IGL03400:Or10a3m	APN	7	108,312,733 (GRCm39)	missense	probably benign	0.28
R0092:Or10a3m	UTSW	7	108,313,031 (GRCm39)	missense	probably benign
R0741:Or10a3m	UTSW	7	108,312,811 (GRCm39)	missense	probably benign	0.00
R1515:Or10a3m	UTSW	7	108,313,148 (GRCm39)	missense	possibly damaging	0.94
R1982:Or10a3m	UTSW	7	108,312,902 (GRCm39)	missense	probably damaging	1.00
R2176:Or10a3m	UTSW	7	108,313,339 (GRCm39)	missense	probably damaging	1.00
R3967:Or10a3m	UTSW	7	108,313,060 (GRCm39)	missense	probably benign
R4009:Or10a3m	UTSW	7	108,313,366 (GRCm39)	missense	probably benign	0.00
R4010:Or10a3m	UTSW	7	108,313,366 (GRCm39)	missense	probably benign	0.00
R4011:Or10a3m	UTSW	7	108,313,366 (GRCm39)	missense	probably benign	0.00
R5095:Or10a3m	UTSW	7	108,313,019 (GRCm39)	missense	probably damaging	1.00
R5271:Or10a3m	UTSW	7	108,313,424 (GRCm39)	missense	probably damaging	1.00
R5864:Or10a3m	UTSW	7	108,312,671 (GRCm39)	missense	probably benign
R5926:Or10a3m	UTSW	7	108,312,794 (GRCm39)	missense	probably damaging	1.00
R6295:Or10a3m	UTSW	7	108,312,845 (GRCm39)	missense	probably damaging	0.98
R6528:Or10a3m	UTSW	7	108,312,638 (GRCm39)	missense	probably damaging	1.00
R6624:Or10a3m	UTSW	7	108,312,743 (GRCm39)	missense	possibly damaging	0.50
R8029:Or10a3m	UTSW	7	108,313,037 (GRCm39)	missense	possibly damaging	0.70
R8443:Or10a3m	UTSW	7	108,313,418 (GRCm39)	missense	possibly damaging	0.79
R9415:Or10a3m	UTSW	7	108,313,042 (GRCm39)	missense	probably damaging	1.00
R9622:Or10a3m	UTSW	7	108,312,677 (GRCm39)	missense	probably benign	0.30
X0023:Or10a3m	UTSW	7	108,313,217 (GRCm39)	missense	possibly damaging	0.79
Z1088:Or10a3m	UTSW	7	108,312,745 (GRCm39)	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- GTCTGCACATCCCCATGTAC -3'
(R):5'- CAGTTCTAGCACAGGAGGAGTC -3'

Sequencing Primer
(F):5'- ACATCCCCATGTACCTATTTCTGCAG -3'
(R):5'- TCTAGCACAGGAGGAGTCTCACAG -3'

Posted On

2021-03-08