Mutagenetix > Incidental Mutation

Incidental Mutation 'R8742:Mslnl'

663311

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8742 (G1)

Quality Score

164.009

Status

Validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 25745073 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 459 (I459F)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098] [ENSMUST00000075884]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: I459F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: I459F

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000075884

SMART Domains

Protein: ENSMUSP00000075279
Gene: ENSMUSG00000063011

Domain	Start	End	E-Value	Type
Pfam:Mesothelin	1	624	N/A	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.0%

Validation Efficiency

100% (55/55)

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5430419D17Rik	A	G	7: 131,182,012	I45V	unknown	Het
Adgrb3	T	C	1: 25,226,754	I893V	probably benign	Het
Adhfe1	A	G	1: 9,560,176	I317V	probably benign	Het
Adnp2	A	G	18: 80,128,341	V951A	probably damaging	Het
Ankrd35	T	A	3: 96,679,186	H59Q	probably damaging	Het
Atg16l1	C	T	1: 87,766,898	T161I	probably damaging	Het
Axl	A	G	7: 25,764,436	V591A	probably damaging	Het
C87414	T	A	5: 93,638,076	D115V	probably damaging	Het
Cdc40	C	T	10: 40,841,484	D404N	probably damaging	Het
Ceacam13	T	A	7: 18,010,009	S14T	probably damaging	Het
Cfh	A	T	1: 140,101,652	F986L	probably damaging	Het
Cfh	G	T	1: 140,136,731	T393K	probably damaging	Het
Clic1	A	G	17: 35,055,380	N179S	probably benign	Het
Col6a3	C	G	1: 90,767,606		probably benign	Het
Dlat	G	T	9: 50,649,667	A360E	probably damaging	Het
Dst	T	A	1: 34,212,344	D2159E	probably benign	Het
Epn3	T	G	11: 94,496,095	T150P	probably damaging	Het
Fgr	T	C	4: 132,997,517	Y310H	probably damaging	Het
Filip1l	A	G	16: 57,571,230	Y727C	probably damaging	Het
Fis1	G	T	5: 136,953,511		probably benign	Het
G530012D18Rik	C	G	1: 85,577,214	D113E	unknown	Het
Gdf10	A	T	14: 33,932,469	H311L	probably benign	Het
Gphn	C	T	12: 78,612,992	R423C	probably damaging	Het
Gpr132	T	C	12: 112,855,897		probably benign	Het
Herc2	C	A	7: 56,094,395	T406K	probably benign	Het
Hpf1	T	C	8: 60,893,714	V21A	probably benign	Het
Ifitm6	A	G	7: 141,016,095	I75T	probably benign	Het
Itgax	A	T	7: 128,144,623	H852L	probably benign	Het
Klhl3	T	C	13: 58,011,207	Y546C	probably damaging	Het
Lbr	A	G	1: 181,817,006	L578P	possibly damaging	Het
Ltbp1	A	G	17: 75,310,222	Y734C	probably damaging	Het
Mapre2	C	A	18: 23,883,631	H281N	probably benign	Het
Mcm4	T	C	16: 15,625,566	D831G	possibly damaging	Het
Mlf1	G	A	3: 67,397,786	A207T	probably damaging	Het
Mtnr1a	A	G	8: 45,087,683	D227G	probably benign	Het
Ncapg	T	A	5: 45,693,874	L803Q	probably damaging	Het
Nr3c2	T	G	8: 76,908,581	S104A	probably damaging	Het
Olfr1302	A	G	2: 111,780,565	I82V	probably benign	Het
Pard3	C	T	8: 127,324,111	A218V	possibly damaging	Het
Poteg	A	T	8: 27,494,929	N439Y	possibly damaging	Het
Ret	A	T	6: 118,178,523	L404Q	probably damaging	Het
Ripk4	A	T	16: 97,755,072	V157D	probably damaging	Het
Rsf1	CGGCGGCGGCGGCGGCGGCGGC	CGGCGGCGGCGGCGGCGGCGGCGGCGGCGGC	7: 97,579,914		probably benign	Het
Serinc1	A	T	10: 57,519,799	N298K	probably benign	Het
Sh3pxd2a	G	A	19: 47,286,634	S230L	probably benign	Het
Shb	G	C	4: 45,458,319	R282G	probably benign	Het
Skint11	T	C	4: 114,194,725	I90T	probably damaging	Het
Slc36a4	A	G	9: 15,720,743	T72A	probably damaging	Het
Slc38a9	T	A	13: 112,729,284	I505K	probably damaging	Het
Sprr3	C	T	3: 92,457,000	R179H	possibly damaging	Het
Syne1	A	T	10: 5,108,661	I7284N	probably benign	Het
Timeless	A	T	10: 128,247,238	T648S	probably benign	Het
Trim44	A	G	2: 102,400,176	M170T	possibly damaging	Het
Trpm7	A	G	2: 126,825,549	F815L	probably damaging	Het
Uimc1	A	G	13: 55,093,158	L39S	possibly damaging	Het
Vmn2r13	T	C	5: 109,156,397	T723A	probably benign	Het
Vps11	A	G	9: 44,355,773		probably benign	Het
Zp3r	A	T	1: 130,583,493	C383S	probably damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25743667	unclassified	probably benign
IGL01629:Mslnl	APN	17	25744775	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25746151	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25747998	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25744103	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25744077	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25743203	nonsense	probably null
R0881:Mslnl	UTSW	17	25742965	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25744517	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25744969	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25738978	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25738968	nonsense	probably null
R5257:Mslnl	UTSW	17	25746165	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25743159	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25737842	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25746775	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25737902	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25744557	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25746073	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25743212	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25743210	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25736921	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25743183	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25746777	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25746988	missense	probably benign
R8735:Mslnl	UTSW	17	25745088	missense	probably benign	0.09
R9208:Mslnl	UTSW	17	25742720	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25742532	intron	probably benign
RF007:Mslnl	UTSW	17	25743228	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- GGTGATAGGGCTGGCAAC -3'
(R):5'- TCACTCAGTTTGTAATGCTTCTTTCA -3'

Sequencing Primer
(F):5'- TTGGACCACAATGGCAAG -3'
(R):5'- TGTTGCAAGCACTCTACAGG -3'

Posted On

2021-03-08