Mutagenetix > Incidental Mutation

Incidental Mutation 'R8755:Or13a21'

664108

Institutional Source

Beutler Lab

Gene Symbol

Or13a21

Ensembl Gene

ENSMUSG00000063823

Gene Name

olfactory receptor family 13 subfamily A member 21

Synonyms

Olfr532, GA_x6K02T2PBJ9-42570051-42569122, MOR251-1

MMRRC Submission

068596-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.061) question?

Stock #

R8755 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

139998755-139999684 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 139999417 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Arginine at position 90 (S90R)

Ref Sequence

ENSEMBL: ENSMUSP00000150798 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073226] [ENSMUST00000213801]

AlphaFold

Q8VGT4

Predicted Effect

probably benign
Transcript: ENSMUST00000073226
AA Change: S90R

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000072959
Gene: ENSMUSG00000063823
AA Change: S90R

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	4.7e-51	PFAM
Pfam:7TM_GPCR_Srsx	35	305	2.5e-6	PFAM
Pfam:7tm_1	41	290	1.7e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213801
AA Change: S90R

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aaas	A	C	15: 102,255,520 (GRCm39)	D86E	probably benign	Het
Akap3	A	G	6: 126,843,130 (GRCm39)	D583G	possibly damaging	Het
Alms1	T	A	6: 85,598,556 (GRCm39)	D1127E	probably benign	Het
Anks4b	T	A	7: 119,773,307 (GRCm39)		probably null	Het
Ano8	A	T	8: 71,935,724 (GRCm39)	F298Y	probably benign	Het
Arhgef2	C	A	3: 88,536,850 (GRCm39)	Q7K	probably benign	Het
Brd10	T	C	19: 29,693,890 (GRCm39)	I1868V	probably benign	Het
Cand2	T	C	6: 115,769,941 (GRCm39)	L917P	probably damaging	Het
Car8	T	C	4: 8,238,083 (GRCm39)	D44G	probably benign	Het
Cdk1	T	C	10: 69,176,435 (GRCm39)	N224S	probably benign	Het
Cep162	T	C	9: 87,114,064 (GRCm39)	E336G	probably benign	Het
Chd7	T	C	4: 8,866,069 (GRCm39)	M2792T	probably benign	Het
Cyp4f40	A	T	17: 32,886,957 (GRCm39)	K143*	probably null	Het
Cyth1	T	A	11: 118,074,768 (GRCm39)	M178L	probably benign	Het
Dcaf11	C	T	14: 55,798,023 (GRCm39)		probably benign	Het
Dlec1	T	G	9: 118,967,225 (GRCm39)	W1203G	probably damaging	Het
Fgd4	A	T	16: 16,302,133 (GRCm39)	S141T	probably benign	Het
Firrm	A	G	1: 163,786,895 (GRCm39)	S725P	probably damaging	Het
Flg2	T	A	3: 93,108,120 (GRCm39)	D49E	probably damaging	Het
Fyb2	T	A	4: 104,861,086 (GRCm39)	D647E	unknown	Het
Gstp1	T	C	19: 4,086,698 (GRCm39)	Y109C	probably damaging	Het
Gtf3c3	T	C	1: 54,468,031 (GRCm39)	E202G	probably benign	Het
Hephl1	A	G	9: 14,985,563 (GRCm39)	F698L	probably benign	Het
Hephl1	G	T	9: 15,023,280 (GRCm39)	P41T	probably damaging	Het
Hmcn1	T	A	1: 150,509,371 (GRCm39)	E3659V	probably benign	Het
Il27ra	A	T	8: 84,765,988 (GRCm39)	D229E	probably damaging	Het
Itih2	T	A	2: 10,103,369 (GRCm39)	D706V	probably damaging	Het
Itpr2	A	T	6: 146,133,926 (GRCm39)	C1893S	probably benign	Het
Lama5	T	C	2: 179,832,714 (GRCm39)	N1646S	probably benign	Het
Laptm4b	A	G	15: 34,273,420 (GRCm39)	D111G	probably damaging	Het
Ldb3	T	A	14: 34,299,256 (GRCm39)	S123C	probably damaging	Het
Lgals12	C	T	19: 7,581,345 (GRCm39)	E121K	possibly damaging	Het
Mmp13	T	A	9: 7,277,996 (GRCm39)	S296R	possibly damaging	Het
Mpeg1	T	C	19: 12,439,238 (GRCm39)	F232S	probably damaging	Het
Ncapd2	A	T	6: 125,148,817 (GRCm39)	C990S	possibly damaging	Het
Nckap5	A	T	1: 125,954,279 (GRCm39)	C758S	possibly damaging	Het
Nwd1	A	T	8: 73,394,192 (GRCm39)	D485V	probably damaging	Het
Or6c2	T	C	10: 129,362,332 (GRCm39)	F79L	possibly damaging	Het
Or8g50	T	A	9: 39,648,786 (GRCm39)	I225N	probably damaging	Het
Pcgf3	G	A	5: 108,634,108 (GRCm39)	R122Q	probably benign	Het
Pgm5	T	A	19: 24,812,212 (GRCm39)	I107F	probably damaging	Het
Pid1	G	T	1: 84,016,066 (GRCm39)	H114N	probably damaging	Het
Pkdrej	T	C	15: 85,703,807 (GRCm39)	T710A	probably benign	Het
Plpbp	T	A	8: 27,535,165 (GRCm39)		probably null	Het
Ppfibp2	T	G	7: 107,343,432 (GRCm39)	F824V	probably damaging	Het
Ppp1r16b	G	A	2: 158,593,098 (GRCm39)	D226N	probably damaging	Het
Psg17	T	A	7: 18,550,836 (GRCm39)	T340S	possibly damaging	Het
Pstpip2	T	C	18: 77,961,133 (GRCm39)	S239P	probably damaging	Het
Ralbp1	A	C	17: 66,166,036 (GRCm39)	S383A	possibly damaging	Het
Ranbp2	T	A	10: 58,300,969 (GRCm39)	L613*	probably null	Het
Rgs11	T	C	17: 26,422,346 (GRCm39)	V49A	probably damaging	Het
Ryr1	C	T	7: 28,791,693 (GRCm39)	V1404I	probably benign	Het
Secisbp2	G	A	13: 51,833,869 (GRCm39)	V670I	possibly damaging	Het
Slc22a13	T	A	9: 119,038,126 (GRCm39)	M1L	probably damaging	Het
Slc5a1	A	T	5: 33,316,526 (GRCm39)	I591L	probably benign	Het
Tacr2	T	A	10: 62,088,733 (GRCm39)	V46E	possibly damaging	Het
Tet2	C	T	3: 133,194,039 (GRCm39)	G132S	probably damaging	Het
Thsd7a	G	A	6: 12,408,851 (GRCm39)	R724*	probably null	Het
Tmco3	A	T	8: 13,341,782 (GRCm39)	I19L	probably benign	Het
Tnfrsf1a	T	A	6: 125,334,768 (GRCm39)	L14Q	probably benign	Het
Tpm1	A	T	9: 66,935,371 (GRCm39)	L248Q	probably benign	Het
Tprn	T	A	2: 25,154,027 (GRCm39)	I443N	probably benign	Het
Unc80	A	T	1: 66,651,290 (GRCm39)	H1545L	possibly damaging	Het
Vmn2r97	T	A	17: 19,168,104 (GRCm39)	M786K	probably damaging	Het
Wipi1	C	T	11: 109,494,645 (GRCm39)	V63M	probably damaging	Het
Zcchc4	G	A	5: 52,976,724 (GRCm39)	R506H	unknown	Het
Zfand6	A	T	7: 84,281,899 (GRCm39)	V110E	probably benign	Het
Zfp866	A	T	8: 70,219,381 (GRCm39)	Y80N	possibly damaging	Het

Other mutations in Or13a21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01684:Or13a21	APN	7	139,998,828 (GRCm39)	missense	probably damaging	1.00
IGL01743:Or13a21	APN	7	139,999,581 (GRCm39)	missense	probably damaging	1.00
IGL01797:Or13a21	APN	7	139,998,931 (GRCm39)	missense	probably damaging	1.00
IGL02291:Or13a21	APN	7	139,999,200 (GRCm39)	missense	probably damaging	1.00
IGL02382:Or13a21	APN	7	139,999,516 (GRCm39)	missense	possibly damaging	0.72
IGL02514:Or13a21	APN	7	139,999,507 (GRCm39)	missense	probably damaging	1.00
IGL02600:Or13a21	APN	7	139,998,862 (GRCm39)	missense	probably benign
IGL02613:Or13a21	APN	7	139,999,383 (GRCm39)	missense	probably benign	0.04
R0358:Or13a21	UTSW	7	139,998,856 (GRCm39)	missense	probably damaging	0.98
R0827:Or13a21	UTSW	7	139,999,380 (GRCm39)	missense	probably damaging	0.99
R1464:Or13a21	UTSW	7	139,999,286 (GRCm39)	missense	probably benign	0.01
R1464:Or13a21	UTSW	7	139,999,286 (GRCm39)	missense	probably benign	0.01
R1539:Or13a21	UTSW	7	139,999,326 (GRCm39)	missense	probably benign	0.26
R1691:Or13a21	UTSW	7	139,998,855 (GRCm39)	missense	probably damaging	1.00
R2012:Or13a21	UTSW	7	139,999,024 (GRCm39)	missense	probably damaging	1.00
R2195:Or13a21	UTSW	7	139,999,138 (GRCm39)	missense	possibly damaging	0.49
R4519:Or13a21	UTSW	7	139,999,123 (GRCm39)	missense	probably damaging	1.00
R6368:Or13a21	UTSW	7	139,999,580 (GRCm39)	nonsense	probably null
R6656:Or13a21	UTSW	7	139,999,517 (GRCm39)	missense	probably damaging	0.99
R7467:Or13a21	UTSW	7	139,999,287 (GRCm39)	missense	probably benign
R7610:Or13a21	UTSW	7	139,999,466 (GRCm39)	nonsense	probably null
R7795:Or13a21	UTSW	7	139,999,027 (GRCm39)	missense	possibly damaging	0.49
R7837:Or13a21	UTSW	7	139,999,234 (GRCm39)	missense	probably benign	0.01
R9706:Or13a21	UTSW	7	139,999,266 (GRCm39)	missense	probably damaging	0.96

Predicted Primers

PCR Primer
(F):5'- GAAATTGAGTCGCACCAGCAG -3'
(R):5'- TTACATCCTGGCTCTTGCAG -3'

Sequencing Primer
(F):5'- CACAGATGTGTTGGCTATGCC -3'
(R):5'- ATCCTGGCTCTTGCAGGAAAC -3'

Posted On

2021-03-08