Incidental Mutation 'R8755:Gstp1'
ID 664141
Institutional Source Beutler Lab
Gene Symbol Gstp1
Ensembl Gene ENSMUSG00000060803
Gene Name glutathione S-transferase, pi 1
Synonyms Gst p-1, GstpiB
MMRRC Submission 068596-MU
Accession Numbers
Essential gene? Possibly non essential (E-score: 0.331) question?
Stock # R8755 (G1)
Quality Score 225.009
Status Not validated
Chromosome 19
Chromosomal Location 4085411-4087912 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 4086698 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Cysteine at position 109 (Y109C)
Ref Sequence ENSEMBL: ENSMUSP00000129565 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000042700] [ENSMUST00000169613]
AlphaFold P19157
PDB Structure GLUTATHIONE S-TRANSFERASE YFYF CYS 47-CARBOXYMETHYLATED CLASS PI, FREE ENZYME [X-RAY DIFFRACTION]
1.8 ANGSTROMS MOLECULAR STRUCTURE OF MOUSE LIVER CLASS PI GLUTATHIONE S-TRANSFERASE COMPLEXED WITH S-(P-NITROBENZYL)GLUTATHIONE AND OTHER INHIBITORS [X-RAY DIFFRACTION]
1.8 ANGSTROMS MOLECULAR STRUCTURE OF MOUSE LIVER CLASS PI GLUTATHIONE S-TRANSFERASE COMPLEXED WITH S-(P-NITROBENZYL)GLUTATHIONE AND OTHER INHIBITORS [X-RAY DIFFRACTION]
GLUTATHIONE S-TRANSFERASE YFYF, CLASS PI, COMPLEXED WITH GLUTATHIONE [X-RAY DIFFRACTION]
MODIFIED GLUTATHIONE S-TRANSFERASE (PI) COMPLEXED WITH S (P-NITROBENZYL)GLUTATHIONE [X-RAY DIFFRACTION]
MOLECULAR STRUCTURE AT 1.8 ANGSTROMS OF MOUSE LIVER CLASS PI GLUTATHIONE S-TRANSFERASE COMPLEXED WITH S-(P-NITROBENZYL)GLUTATHIONE AND OTHER INHIBITORS [X-RAY DIFFRACTION]
Mouse C14A Glutathione-S-Transferase Mutant in Complex with S-hexyl glutathione [X-RAY DIFFRACTION]
Mouse C14A Glutathione-S-Transferase Mutant in Complex with S-(p-nitrobenzyl) Glutathione [X-RAY DIFFRACTION]
Structure of Glutathione-S-Transferase C169A Mutant [X-RAY DIFFRACTION]
1.8 Angstroms molecular structure of mouse liver glutathione S-transferase mutant C47A complexed with S-(P-nitrobenzyl)glutathione [X-RAY DIFFRACTION]
Predicted Effect probably benign
Transcript: ENSMUST00000042700
SMART Domains Protein: ENSMUSP00000038931
Gene: ENSMUSG00000038155

DomainStartEndE-ValueType
Pfam:GST_N 2 75 4.2e-8 PFAM
Pfam:GST_C 97 188 1.4e-15 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000169613
AA Change: Y109C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000129565
Gene: ENSMUSG00000060803
AA Change: Y109C

DomainStartEndE-ValueType
Pfam:GST_N 4 75 2.3e-8 PFAM
Pfam:GST_C 97 188 1.5e-15 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.4%
  • 20x: 98.0%
Validation Efficiency
MGI Phenotype PHENOTYPE: Mutant mice with null mutations in both Gstp1 and Gstp2 exhibit an increased susceptibility to DMBA and TPA induced skin papillomas. Male mutant mice exhibit an increased body weight with age. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 68 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aaas A C 15: 102,255,520 (GRCm39) D86E probably benign Het
Akap3 A G 6: 126,843,130 (GRCm39) D583G possibly damaging Het
Alms1 T A 6: 85,598,556 (GRCm39) D1127E probably benign Het
Anks4b T A 7: 119,773,307 (GRCm39) probably null Het
Ano8 A T 8: 71,935,724 (GRCm39) F298Y probably benign Het
Arhgef2 C A 3: 88,536,850 (GRCm39) Q7K probably benign Het
Brd10 T C 19: 29,693,890 (GRCm39) I1868V probably benign Het
Cand2 T C 6: 115,769,941 (GRCm39) L917P probably damaging Het
Car8 T C 4: 8,238,083 (GRCm39) D44G probably benign Het
Cdk1 T C 10: 69,176,435 (GRCm39) N224S probably benign Het
Cep162 T C 9: 87,114,064 (GRCm39) E336G probably benign Het
Chd7 T C 4: 8,866,069 (GRCm39) M2792T probably benign Het
Cyp4f40 A T 17: 32,886,957 (GRCm39) K143* probably null Het
Cyth1 T A 11: 118,074,768 (GRCm39) M178L probably benign Het
Dcaf11 C T 14: 55,798,023 (GRCm39) probably benign Het
Dlec1 T G 9: 118,967,225 (GRCm39) W1203G probably damaging Het
Fgd4 A T 16: 16,302,133 (GRCm39) S141T probably benign Het
Firrm A G 1: 163,786,895 (GRCm39) S725P probably damaging Het
Flg2 T A 3: 93,108,120 (GRCm39) D49E probably damaging Het
Fyb2 T A 4: 104,861,086 (GRCm39) D647E unknown Het
Gtf3c3 T C 1: 54,468,031 (GRCm39) E202G probably benign Het
Hephl1 A G 9: 14,985,563 (GRCm39) F698L probably benign Het
Hephl1 G T 9: 15,023,280 (GRCm39) P41T probably damaging Het
Hmcn1 T A 1: 150,509,371 (GRCm39) E3659V probably benign Het
Il27ra A T 8: 84,765,988 (GRCm39) D229E probably damaging Het
Itih2 T A 2: 10,103,369 (GRCm39) D706V probably damaging Het
Itpr2 A T 6: 146,133,926 (GRCm39) C1893S probably benign Het
Lama5 T C 2: 179,832,714 (GRCm39) N1646S probably benign Het
Laptm4b A G 15: 34,273,420 (GRCm39) D111G probably damaging Het
Ldb3 T A 14: 34,299,256 (GRCm39) S123C probably damaging Het
Lgals12 C T 19: 7,581,345 (GRCm39) E121K possibly damaging Het
Mmp13 T A 9: 7,277,996 (GRCm39) S296R possibly damaging Het
Mpeg1 T C 19: 12,439,238 (GRCm39) F232S probably damaging Het
Ncapd2 A T 6: 125,148,817 (GRCm39) C990S possibly damaging Het
Nckap5 A T 1: 125,954,279 (GRCm39) C758S possibly damaging Het
Nwd1 A T 8: 73,394,192 (GRCm39) D485V probably damaging Het
Or13a21 T G 7: 139,999,417 (GRCm39) S90R probably benign Het
Or6c2 T C 10: 129,362,332 (GRCm39) F79L possibly damaging Het
Or8g50 T A 9: 39,648,786 (GRCm39) I225N probably damaging Het
Pcgf3 G A 5: 108,634,108 (GRCm39) R122Q probably benign Het
Pgm5 T A 19: 24,812,212 (GRCm39) I107F probably damaging Het
Pid1 G T 1: 84,016,066 (GRCm39) H114N probably damaging Het
Pkdrej T C 15: 85,703,807 (GRCm39) T710A probably benign Het
Plpbp T A 8: 27,535,165 (GRCm39) probably null Het
Ppfibp2 T G 7: 107,343,432 (GRCm39) F824V probably damaging Het
Ppp1r16b G A 2: 158,593,098 (GRCm39) D226N probably damaging Het
Psg17 T A 7: 18,550,836 (GRCm39) T340S possibly damaging Het
Pstpip2 T C 18: 77,961,133 (GRCm39) S239P probably damaging Het
Ralbp1 A C 17: 66,166,036 (GRCm39) S383A possibly damaging Het
Ranbp2 T A 10: 58,300,969 (GRCm39) L613* probably null Het
Rgs11 T C 17: 26,422,346 (GRCm39) V49A probably damaging Het
Ryr1 C T 7: 28,791,693 (GRCm39) V1404I probably benign Het
Secisbp2 G A 13: 51,833,869 (GRCm39) V670I possibly damaging Het
Slc22a13 T A 9: 119,038,126 (GRCm39) M1L probably damaging Het
Slc5a1 A T 5: 33,316,526 (GRCm39) I591L probably benign Het
Tacr2 T A 10: 62,088,733 (GRCm39) V46E possibly damaging Het
Tet2 C T 3: 133,194,039 (GRCm39) G132S probably damaging Het
Thsd7a G A 6: 12,408,851 (GRCm39) R724* probably null Het
Tmco3 A T 8: 13,341,782 (GRCm39) I19L probably benign Het
Tnfrsf1a T A 6: 125,334,768 (GRCm39) L14Q probably benign Het
Tpm1 A T 9: 66,935,371 (GRCm39) L248Q probably benign Het
Tprn T A 2: 25,154,027 (GRCm39) I443N probably benign Het
Unc80 A T 1: 66,651,290 (GRCm39) H1545L possibly damaging Het
Vmn2r97 T A 17: 19,168,104 (GRCm39) M786K probably damaging Het
Wipi1 C T 11: 109,494,645 (GRCm39) V63M probably damaging Het
Zcchc4 G A 5: 52,976,724 (GRCm39) R506H unknown Het
Zfand6 A T 7: 84,281,899 (GRCm39) V110E probably benign Het
Zfp866 A T 8: 70,219,381 (GRCm39) Y80N possibly damaging Het
Other mutations in Gstp1
AlleleSourceChrCoordTypePredicted EffectPPH Score
R1658:Gstp1 UTSW 19 4,087,375 (GRCm39) missense probably damaging 0.99
R1848:Gstp1 UTSW 19 4,086,795 (GRCm39) splice site probably benign
R3432:Gstp1 UTSW 19 4,086,695 (GRCm39) missense possibly damaging 0.50
R6634:Gstp1 UTSW 19 4,085,510 (GRCm39) missense probably benign 0.21
RF021:Gstp1 UTSW 19 4,085,507 (GRCm39) missense probably benign 0.01
Predicted Primers PCR Primer
(F):5'- TGACCTCTCCTGAAGCTGTC -3'
(R):5'- TCTTGTTCAGGCTGCATCTG -3'

Sequencing Primer
(F):5'- TGAAGCTGTCTATTGGCCC -3'
(R):5'- TCATGAGCTCGGGTCTCACATG -3'
Posted On 2021-03-08