Mutagenetix > Incidental Mutation

Incidental Mutation 'R8769:Muc5ac'

664293

Institutional Source

Beutler Lab

Gene Symbol

Muc5ac

Ensembl Gene

ENSMUSG00000037974

Gene Name

mucin 5, subtypes A and C, tracheobronchial/gastric

Synonyms

MGM, 2210005L13Rik

MMRRC Submission

068624-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8769 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

141342709-141372968 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 141372609 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Arginine at position 3452 (G3452R)

Ref Sequence

ENSEMBL: ENSMUSP00000122353 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000041924] [ENSMUST00000155534] [ENSMUST00000163321]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000041924
AA Change: G2748R

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000039699
Gene: ENSMUSG00000037974
AA Change: G2748R

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
VWD	69	226	4.19e-30	SMART
C8	258	334	1.34e-11	SMART
Pfam:TIL	337	393	1.6e-14	PFAM
VWC	395	462	8.6e-18	SMART
VWD	421	585	1.55e-33	SMART
C8	622	696	8.42e-36	SMART
Pfam:TIL	702	759	6.1e-9	PFAM
VWC	761	825	6.75e-1	SMART
VWC	863	905	4.06e-1	SMART
VWD	890	1050	1.51e-45	SMART
C8	1086	1160	2.78e-36	SMART
low complexity region	1315	1330	N/A	INTRINSIC
low complexity region	1333	1366	N/A	INTRINSIC
low complexity region	1371	1387	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1394	1482	2.3e-25	PFAM
low complexity region	1521	1532	N/A	INTRINSIC
low complexity region	1536	1563	N/A	INTRINSIC
low complexity region	1578	1595	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1604	1692	2.2e-27	PFAM
Pfam:Mucin2_WxxW	1765	1857	8.6e-27	PFAM
low complexity region	1875	1895	N/A	INTRINSIC
low complexity region	1949	1968	N/A	INTRINSIC
VWD	2030	2199	4.17e-48	SMART
C8	2242	2311	3.95e-9	SMART
VWC	2376	2439	1.04e-11	SMART
VWC	2479	2543	9.31e-5	SMART
CT	2625	2711	3.43e-32	SMART
low complexity region	2720	2733	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000155534
AA Change: G3452R

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000122353
Gene: ENSMUSG00000037974
AA Change: G3452R

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
VWD	69	226	4.19e-30	SMART
C8	258	334	1.34e-11	SMART
Pfam:TIL	337	393	9.6e-15	PFAM
VWC	395	437	3.54e-1	SMART
VWD	422	586	2.35e-33	SMART
C8	623	697	8.42e-36	SMART
Pfam:TIL	703	760	3.6e-9	PFAM
VWC	762	826	6.75e-1	SMART
VWC	864	906	4.06e-1	SMART
VWD	891	1051	1.51e-45	SMART
C8	1087	1161	2.78e-36	SMART
low complexity region	1316	1331	N/A	INTRINSIC
low complexity region	1334	1367	N/A	INTRINSIC
low complexity region	1372	1388	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1395	1483	1.3e-25	PFAM
low complexity region	1522	1533	N/A	INTRINSIC
low complexity region	1537	1564	N/A	INTRINSIC
low complexity region	1579	1596	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1605	1693	1.3e-27	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000163321
AA Change: G2748R

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000131681
Gene: ENSMUSG00000037974
AA Change: G2748R

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
VWD	69	226	4.19e-30	SMART
C8	258	334	1.34e-11	SMART
Pfam:TIL	337	393	7.9e-15	PFAM
VWC	395	462	8.6e-18	SMART
VWD	421	585	1.55e-33	SMART
C8	622	696	8.42e-36	SMART
Pfam:TIL	702	759	1.9e-9	PFAM
VWC	761	825	6.75e-1	SMART
VWC	863	905	4.06e-1	SMART
VWD	890	1050	1.51e-45	SMART
C8	1086	1160	2.78e-36	SMART
low complexity region	1315	1330	N/A	INTRINSIC
low complexity region	1333	1366	N/A	INTRINSIC
low complexity region	1371	1387	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1394	1481	1.1e-23	PFAM
low complexity region	1521	1532	N/A	INTRINSIC
low complexity region	1536	1563	N/A	INTRINSIC
low complexity region	1578	1595	N/A	INTRINSIC
Pfam:Mucin2_WxxW	1604	1691	1.1e-25	PFAM
Pfam:Mucin2_WxxW	1765	1856	6.3e-24	PFAM
low complexity region	1875	1895	N/A	INTRINSIC
low complexity region	1949	1968	N/A	INTRINSIC
VWD	2030	2199	4.17e-48	SMART
C8	2242	2311	3.95e-9	SMART
VWC	2376	2439	1.04e-11	SMART
VWC	2479	2543	9.31e-5	SMART
CT	2625	2711	3.43e-32	SMART
low complexity region	2720	2733	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

97% (57/59)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased susceptibility to T. muris infection with persistent worm burden, goblet cell hyperplasia, and increased serum IFN-gamma despite a normal TH2-type immune response. A portion of mice show corneal opacity and poor tear quality. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933409G03Rik	T	A	2: 68,446,589 (GRCm39)	N289K	unknown	Het
Adgrl2	T	C	3: 148,522,917 (GRCm39)	I436V		Het
Agbl3	C	T	6: 34,834,549 (GRCm39)	S911L	probably damaging	Het
Ampd2	C	T	3: 107,982,613 (GRCm39)	M714I	probably damaging	Het
Asb6	T	C	2: 30,718,143 (GRCm39)	D19G	possibly damaging	Het
Atosa	C	A	9: 74,933,107 (GRCm39)	L1025I	probably damaging	Het
Bbx	T	C	16: 50,061,227 (GRCm39)	Q298R	probably damaging	Het
Bcl9l	T	A	9: 44,420,263 (GRCm39)	M1223K	probably benign	Het
Ccr3	T	A	9: 123,829,096 (GRCm39)	F144I	possibly damaging	Het
Cfap74	T	A	4: 155,503,105 (GRCm39)	F32L		Het
Clhc1	G	A	11: 29,511,401 (GRCm39)	E293K	probably damaging	Het
Col22a1	A	T	15: 71,878,571 (GRCm39)	D195E	probably benign	Het
Cttnbp2	T	A	6: 18,376,003 (GRCm39)	D1512V	probably damaging	Het
Cul9	T	C	17: 46,832,828 (GRCm39)	T1372A	possibly damaging	Het
Dyrk4	G	T	6: 126,857,208 (GRCm39)	D490E	possibly damaging	Het
E4f1	C	T	17: 24,663,574 (GRCm39)	V626M	probably damaging	Het
Edc3	C	T	9: 57,634,678 (GRCm39)	R232C	probably damaging	Het
Eppk1	A	G	15: 75,994,895 (GRCm39)	I662T	probably benign	Het
G3bp2	C	T	5: 92,231,356 (GRCm39)		probably benign	Het
Grik3	T	C	4: 125,550,166 (GRCm39)	L397P	probably damaging	Het
Hectd4	A	G	5: 121,419,936 (GRCm39)	N627S	possibly damaging	Het
Hecw2	C	T	1: 53,952,507 (GRCm39)	V909I	probably benign	Het
Hsd17b12	A	T	2: 93,945,397 (GRCm39)	M75K	probably damaging	Het
Htt	C	T	5: 34,977,633 (GRCm39)	T809I	probably benign	Het
Itih1	A	G	14: 30,655,381 (GRCm39)	S609P	probably damaging	Het
Klk1b9	T	A	7: 43,629,666 (GRCm39)	C234S	probably damaging	Het
Lrrc37a	A	G	11: 103,389,536 (GRCm39)	L1963P	probably benign	Het
Lypd3	C	A	7: 24,337,932 (GRCm39)	H99Q	probably damaging	Het
Mdn1	C	A	4: 32,751,390 (GRCm39)	H4593Q	probably damaging	Het
Mroh1	A	G	15: 76,297,126 (GRCm39)	S325G	probably benign	Het
Myrfl	A	T	10: 116,612,696 (GRCm39)	D884E	probably damaging	Het
Nbeal1	T	A	1: 60,274,370 (GRCm39)	H260Q	probably damaging	Het
Ngef	A	G	1: 87,408,883 (GRCm39)	L519P	probably damaging	Het
Or5ac20	A	G	16: 59,104,194 (GRCm39)	L222P	probably damaging	Het
Or5b102	T	A	19: 13,041,307 (GRCm39)	C177*	probably null	Het
Or5w1b	A	T	2: 87,475,960 (GRCm39)	F169Y	probably damaging	Het
Pcca	A	G	14: 122,854,260 (GRCm39)	K184R	probably benign	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 110,350,827 (GRCm39)		probably benign	Het
Ppp6r1	A	T	7: 4,644,289 (GRCm39)	V379E	probably benign	Het
Prkca	A	T	11: 107,842,286 (GRCm39)		probably benign	Het
Prune2	A	G	19: 17,100,442 (GRCm39)	E1982G	probably damaging	Het
Rad21l	T	C	2: 151,509,838 (GRCm39)	T88A	probably benign	Het
Rpgrip1l	T	C	8: 91,979,212 (GRCm39)		probably benign	Het
Sec62	T	C	3: 30,864,177 (GRCm39)		probably null	Het
Slc22a19	C	T	19: 7,670,086 (GRCm39)	R256Q	possibly damaging	Het
Slc43a2	G	T	11: 75,434,192 (GRCm39)		probably null	Het
Smarcd3	T	C	5: 24,803,792 (GRCm39)	M30V	probably benign	Het
Tes3-ps	T	A	13: 49,647,356 (GRCm39)	D77E	probably benign	Het
Thbs3	G	A	3: 89,131,937 (GRCm39)		probably benign	Het
Trpm2	A	T	10: 77,768,128 (GRCm39)	N790K	probably benign	Het
Ttc13	A	T	8: 125,405,816 (GRCm39)	N492K	possibly damaging	Het
Ttc39c	T	C	18: 12,828,545 (GRCm39)	L235P	probably damaging	Het
Tyms	T	C	5: 30,278,360 (GRCm39)		probably benign	Het
Whamm	A	T	7: 81,234,933 (GRCm39)	K333*	probably null	Het
Ydjc	A	G	16: 16,968,732 (GRCm39)	I224V	probably benign	Het
Zc3hav1	C	T	6: 38,313,416 (GRCm39)	D210N	possibly damaging	Het
Zfp735	G	A	11: 73,581,127 (GRCm39)	E55K	possibly damaging	Het

Other mutations in Muc5ac

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00899:Muc5ac	APN	7	141,366,440 (GRCm39)	missense	possibly damaging	0.93
IGL01064:Muc5ac	APN	7	141,361,210 (GRCm39)	missense	probably benign	0.12
IGL01155:Muc5ac	APN	7	141,360,680 (GRCm39)	splice site	probably benign
IGL01452:Muc5ac	APN	7	141,371,292 (GRCm39)	missense	probably benign	0.00
IGL01590:Muc5ac	APN	7	141,352,630 (GRCm39)	missense	probably benign	0.02
IGL02104:Muc5ac	APN	7	141,364,815 (GRCm39)	missense	probably damaging	0.98
IGL02152:Muc5ac	APN	7	141,353,914 (GRCm39)	missense	possibly damaging	0.86
IGL02153:Muc5ac	APN	7	141,372,537 (GRCm39)	nonsense	probably null
IGL02178:Muc5ac	APN	7	141,359,184 (GRCm39)	splice site	probably benign
IGL02403:Muc5ac	APN	7	141,357,187 (GRCm39)	missense	possibly damaging	0.71
IGL02576:Muc5ac	APN	7	141,370,781 (GRCm39)	missense	probably benign	0.01
IGL02665:Muc5ac	APN	7	141,344,823 (GRCm39)	missense	possibly damaging	0.71
IGL02704:Muc5ac	APN	7	141,349,000 (GRCm39)	missense	possibly damaging	0.71
IGL02808:Muc5ac	APN	7	141,359,512 (GRCm39)	missense	possibly damaging	0.72
IGL03283:Muc5ac	APN	7	141,367,518 (GRCm39)	missense	probably benign	0.34
IGL03384:Muc5ac	APN	7	141,366,140 (GRCm39)	missense	possibly damaging	0.71
IGL03046:Muc5ac	UTSW	7	141,348,950 (GRCm39)	missense	probably benign	0.27
PIT4515001:Muc5ac	UTSW	7	141,361,153 (GRCm39)	missense	probably damaging	0.99
R0092:Muc5ac	UTSW	7	141,372,367 (GRCm39)	missense	possibly damaging	0.72
R0145:Muc5ac	UTSW	7	141,349,012 (GRCm39)	missense	possibly damaging	0.71
R0147:Muc5ac	UTSW	7	141,364,776 (GRCm39)	missense	probably benign	0.08
R0363:Muc5ac	UTSW	7	141,354,697 (GRCm39)	missense	probably benign	0.01
R0384:Muc5ac	UTSW	7	141,365,988 (GRCm39)	missense	possibly damaging	0.71
R0440:Muc5ac	UTSW	7	141,345,771 (GRCm39)	nonsense	probably null
R0583:Muc5ac	UTSW	7	141,361,345 (GRCm39)	missense	probably damaging	0.99
R0616:Muc5ac	UTSW	7	141,349,981 (GRCm39)	missense	probably benign	0.02
R0682:Muc5ac	UTSW	7	141,359,406 (GRCm39)	missense	possibly damaging	0.53
R0685:Muc5ac	UTSW	7	141,361,446 (GRCm39)	missense	probably benign	0.03
R0883:Muc5ac	UTSW	7	141,350,002 (GRCm39)	missense	possibly damaging	0.71
R0924:Muc5ac	UTSW	7	141,361,252 (GRCm39)	missense	possibly damaging	0.68
R1300:Muc5ac	UTSW	7	141,370,666 (GRCm39)	missense	possibly damaging	0.73
R1315:Muc5ac	UTSW	7	141,361,060 (GRCm39)	missense	probably damaging	0.99
R1354:Muc5ac	UTSW	7	141,361,114 (GRCm39)	missense	probably damaging	0.99
R1484:Muc5ac	UTSW	7	141,367,629 (GRCm39)	splice site	probably null
R1599:Muc5ac	UTSW	7	141,352,640 (GRCm39)	missense	possibly damaging	0.52
R1758:Muc5ac	UTSW	7	141,355,268 (GRCm39)	missense	possibly damaging	0.86
R1837:Muc5ac	UTSW	7	141,360,823 (GRCm39)	missense	probably benign	0.00
R1911:Muc5ac	UTSW	7	141,350,041 (GRCm39)	missense	probably benign	0.18
R1922:Muc5ac	UTSW	7	141,347,426 (GRCm39)	missense	probably benign	0.03
R1966:Muc5ac	UTSW	7	141,357,113 (GRCm39)	missense	possibly damaging	0.92
R1994:Muc5ac	UTSW	7	141,366,889 (GRCm39)	missense	possibly damaging	0.93
R2056:Muc5ac	UTSW	7	141,345,772 (GRCm39)	missense	probably benign	0.01
R2126:Muc5ac	UTSW	7	141,364,479 (GRCm39)	missense	possibly damaging	0.84
R2170:Muc5ac	UTSW	7	141,366,084 (GRCm39)	missense	possibly damaging	0.93
R2258:Muc5ac	UTSW	7	141,344,745 (GRCm39)	missense	probably benign	0.41
R2259:Muc5ac	UTSW	7	141,344,745 (GRCm39)	missense	probably benign	0.41
R2293:Muc5ac	UTSW	7	141,360,936 (GRCm39)	missense	probably damaging	0.99
R2435:Muc5ac	UTSW	7	141,371,841 (GRCm39)	missense	possibly damaging	0.53
R2895:Muc5ac	UTSW	7	141,344,877 (GRCm39)	missense	possibly damaging	0.92
R2910:Muc5ac	UTSW	7	141,361,378 (GRCm39)	missense	probably damaging	0.99
R3154:Muc5ac	UTSW	7	141,346,473 (GRCm39)	splice site	probably null
R3762:Muc5ac	UTSW	7	141,361,212 (GRCm39)	missense	possibly damaging	0.53
R3791:Muc5ac	UTSW	7	141,352,238 (GRCm39)	missense	probably benign	0.32
R3806:Muc5ac	UTSW	7	141,367,471 (GRCm39)	missense	possibly damaging	0.91
R3825:Muc5ac	UTSW	7	141,368,460 (GRCm39)	missense	possibly damaging	0.92
R3888:Muc5ac	UTSW	7	141,344,961 (GRCm39)	missense	possibly damaging	0.51
R3929:Muc5ac	UTSW	7	141,356,629 (GRCm39)	missense	probably benign
R3981:Muc5ac	UTSW	7	141,367,512 (GRCm39)	missense	possibly damaging	0.86
R4034:Muc5ac	UTSW	7	141,353,581 (GRCm39)	critical splice donor site	probably null
R4043:Muc5ac	UTSW	7	141,361,215 (GRCm39)	missense	possibly damaging	0.53
R4061:Muc5ac	UTSW	7	141,364,867 (GRCm39)	missense	possibly damaging	0.85
R4106:Muc5ac	UTSW	7	141,356,572 (GRCm39)	missense	possibly damaging	0.86
R4206:Muc5ac	UTSW	7	141,370,847 (GRCm39)	missense	possibly damaging	0.73
R4613:Muc5ac	UTSW	7	141,344,840 (GRCm39)	missense	possibly damaging	0.93
R4719:Muc5ac	UTSW	7	141,343,500 (GRCm39)	missense	possibly damaging	0.83
R4751:Muc5ac	UTSW	7	141,371,338 (GRCm39)	missense	probably benign	0.00
R4789:Muc5ac	UTSW	7	141,352,619 (GRCm39)	missense	possibly damaging	0.86
R4928:Muc5ac	UTSW	7	141,371,639 (GRCm39)	nonsense	probably null
R4971:Muc5ac	UTSW	7	141,370,015 (GRCm39)	missense	possibly damaging	0.68
R4982:Muc5ac	UTSW	7	141,363,193 (GRCm39)	intron	probably benign
R5088:Muc5ac	UTSW	7	141,350,056 (GRCm39)	missense	possibly damaging	0.53
R5141:Muc5ac	UTSW	7	141,368,479 (GRCm39)	missense	possibly damaging	0.72
R5224:Muc5ac	UTSW	7	141,347,708 (GRCm39)	missense	probably benign	0.32
R5366:Muc5ac	UTSW	7	141,361,287 (GRCm39)	missense	probably benign	0.01
R5497:Muc5ac	UTSW	7	141,361,380 (GRCm39)	missense	probably damaging	0.99
R5507:Muc5ac	UTSW	7	141,361,569 (GRCm39)	missense	possibly damaging	0.72
R5643:Muc5ac	UTSW	7	141,347,452 (GRCm39)	critical splice donor site	probably null
R5811:Muc5ac	UTSW	7	141,352,721 (GRCm39)	missense	possibly damaging	0.51
R5946:Muc5ac	UTSW	7	141,371,644 (GRCm39)	missense	possibly damaging	0.73
R5970:Muc5ac	UTSW	7	141,344,406 (GRCm39)	nonsense	probably null
R5977:Muc5ac	UTSW	7	141,350,104 (GRCm39)	missense	possibly damaging	0.73
R6051:Muc5ac	UTSW	7	141,365,594 (GRCm39)	missense	possibly damaging	0.53
R6126:Muc5ac	UTSW	7	141,354,969 (GRCm39)	missense	possibly damaging	0.71
R6159:Muc5ac	UTSW	7	141,369,323 (GRCm39)	missense	possibly damaging	0.53
R6256:Muc5ac	UTSW	7	141,343,532 (GRCm39)	missense	possibly damaging	0.53
R6283:Muc5ac	UTSW	7	141,370,601 (GRCm39)	nonsense	probably null
R6341:Muc5ac	UTSW	7	141,355,229 (GRCm39)	missense	probably damaging	0.99
R6356:Muc5ac	UTSW	7	141,366,416 (GRCm39)	missense	probably benign	0.05
R6481:Muc5ac	UTSW	7	141,362,808 (GRCm39)	intron	probably benign
R6483:Muc5ac	UTSW	7	141,356,591 (GRCm39)	missense	probably benign	0.18
R6627:Muc5ac	UTSW	7	141,362,427 (GRCm39)	intron	probably benign
R6636:Muc5ac	UTSW	7	141,372,342 (GRCm39)	missense	possibly damaging	0.86
R6637:Muc5ac	UTSW	7	141,372,342 (GRCm39)	missense	possibly damaging	0.86
R6656:Muc5ac	UTSW	7	141,357,065 (GRCm39)	missense	probably damaging	0.98
R6721:Muc5ac	UTSW	7	141,352,729 (GRCm39)	missense	possibly damaging	0.71
R6794:Muc5ac	UTSW	7	141,363,289 (GRCm39)	intron	probably benign
R6844:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6847:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6852:Muc5ac	UTSW	7	141,370,644 (GRCm39)	missense	probably benign	0.03
R6862:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6863:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6864:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6865:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6874:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6875:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6876:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6877:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6889:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R6920:Muc5ac	UTSW	7	141,347,035 (GRCm39)	missense	possibly damaging	0.86
R6998:Muc5ac	UTSW	7	141,372,451 (GRCm39)	missense	possibly damaging	0.92
R7017:Muc5ac	UTSW	7	141,363,424 (GRCm39)	intron	probably benign
R7091:Muc5ac	UTSW	7	141,363,424 (GRCm39)	intron	probably benign
R7092:Muc5ac	UTSW	7	141,363,424 (GRCm39)	intron	probably benign
R7092:Muc5ac	UTSW	7	141,363,385 (GRCm39)	intron	probably benign
R7110:Muc5ac	UTSW	7	141,353,559 (GRCm39)	missense	possibly damaging	0.95
R7117:Muc5ac	UTSW	7	141,367,559 (GRCm39)	nonsense	probably null
R7238:Muc5ac	UTSW	7	141,363,424 (GRCm39)	intron	probably benign
R7238:Muc5ac	UTSW	7	141,363,254 (GRCm39)	missense	unknown
R7396:Muc5ac	UTSW	7	141,362,152 (GRCm39)	missense	unknown
R7456:Muc5ac	UTSW	7	141,346,904 (GRCm39)	missense	probably benign	0.32
R7477:Muc5ac	UTSW	7	141,370,019 (GRCm39)	missense	possibly damaging	0.72
R7530:Muc5ac	UTSW	7	141,367,536 (GRCm39)	missense	possibly damaging	0.51
R7545:Muc5ac	UTSW	7	141,362,405 (GRCm39)	missense	unknown
R7604:Muc5ac	UTSW	7	141,363,446 (GRCm39)	missense	unknown
R7635:Muc5ac	UTSW	7	141,359,413 (GRCm39)	missense	probably damaging	0.98
R7635:Muc5ac	UTSW	7	141,359,490 (GRCm39)	missense	possibly damaging	0.53
R7650:Muc5ac	UTSW	7	141,363,159 (GRCm39)	missense	unknown
R7651:Muc5ac	UTSW	7	141,349,991 (GRCm39)	missense	possibly damaging	0.92
R7685:Muc5ac	UTSW	7	141,363,120 (GRCm39)	missense	unknown
R7720:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7749:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7750:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7751:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7754:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7798:Muc5ac	UTSW	7	141,347,778 (GRCm39)	critical splice donor site	probably null
R7835:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7837:Muc5ac	UTSW	7	141,369,700 (GRCm39)	missense	possibly damaging	0.53
R7858:Muc5ac	UTSW	7	141,357,166 (GRCm39)	missense	possibly damaging	0.51
R7866:Muc5ac	UTSW	7	141,349,589 (GRCm39)	missense	probably benign	0.00
R7874:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7876:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7877:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7881:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7884:Muc5ac	UTSW	7	141,363,040 (GRCm39)	missense	unknown
R7921:Muc5ac	UTSW	7	141,363,424 (GRCm39)	intron	probably benign
R7976:Muc5ac	UTSW	7	141,363,528 (GRCm39)	missense	unknown
R8104:Muc5ac	UTSW	7	141,358,520 (GRCm39)	missense	possibly damaging	0.96
R8177:Muc5ac	UTSW	7	141,361,068 (GRCm39)	missense	probably damaging	1.00
R8214:Muc5ac	UTSW	7	141,356,685 (GRCm39)	missense	possibly damaging	0.53
R8292:Muc5ac	UTSW	7	141,363,000 (GRCm39)	missense	unknown
R8386:Muc5ac	UTSW	7	141,361,371 (GRCm39)	missense	possibly damaging	0.93
R8400:Muc5ac	UTSW	7	141,364,213 (GRCm39)	missense	probably damaging	0.99
R8504:Muc5ac	UTSW	7	141,360,892 (GRCm39)	missense	probably damaging	1.00
R8709:Muc5ac	UTSW	7	141,370,663 (GRCm39)	missense	possibly damaging	0.96
R8725:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R8727:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
R8754:Muc5ac	UTSW	7	141,354,008 (GRCm39)	missense	possibly damaging	0.85
R8933:Muc5ac	UTSW	7	141,343,493 (GRCm39)	missense	possibly damaging	0.59
R8939:Muc5ac	UTSW	7	141,347,091 (GRCm39)	missense	probably damaging	0.98
R9049:Muc5ac	UTSW	7	141,362,712 (GRCm39)	missense	unknown
R9124:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9131:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9132:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9135:Muc5ac	UTSW	7	141,352,218 (GRCm39)	missense	probably damaging	0.99
R9156:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9157:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9159:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9160:Muc5ac	UTSW	7	141,363,529 (GRCm39)	missense	unknown
R9161:Muc5ac	UTSW	7	141,353,026 (GRCm39)	missense	possibly damaging	0.53
R9175:Muc5ac	UTSW	7	141,366,093 (GRCm39)	missense	possibly damaging	0.92
R9183:Muc5ac	UTSW	7	141,352,637 (GRCm39)	missense	possibly damaging	0.71
R9218:Muc5ac	UTSW	7	141,361,098 (GRCm39)	missense	probably damaging	0.99
R9219:Muc5ac	UTSW	7	141,370,800 (GRCm39)	nonsense	probably null
R9239:Muc5ac	UTSW	7	141,353,954 (GRCm39)	missense	probably damaging	0.99
R9246:Muc5ac	UTSW	7	141,364,215 (GRCm39)	missense	probably benign	0.11
R9287:Muc5ac	UTSW	7	141,361,626 (GRCm39)	missense	probably damaging	0.99
R9320:Muc5ac	UTSW	7	141,369,255 (GRCm39)	missense	probably benign	0.01
R9327:Muc5ac	UTSW	7	141,365,429 (GRCm39)	missense	possibly damaging	0.86
R9428:Muc5ac	UTSW	7	141,362,559 (GRCm39)	missense	unknown
R9430:Muc5ac	UTSW	7	141,362,569 (GRCm39)	missense	unknown
R9454:Muc5ac	UTSW	7	141,362,431 (GRCm39)	missense	unknown
R9483:Muc5ac	UTSW	7	141,365,465 (GRCm39)	nonsense	probably null
R9581:Muc5ac	UTSW	7	141,363,799 (GRCm39)	missense	unknown
R9610:Muc5ac	UTSW	7	141,350,078 (GRCm39)	missense	possibly damaging	0.86
R9642:Muc5ac	UTSW	7	141,349,601 (GRCm39)	missense	possibly damaging	0.71
R9684:Muc5ac	UTSW	7	141,364,798 (GRCm39)	missense	probably benign	0.41
R9760:Muc5ac	UTSW	7	141,360,985 (GRCm39)	missense	probably benign	0.05
R9778:Muc5ac	UTSW	7	141,349,021 (GRCm39)	nonsense	probably null
X0060:Muc5ac	UTSW	7	141,357,070 (GRCm39)	missense	possibly damaging	0.71
Z1088:Muc5ac	UTSW	7	141,365,429 (GRCm39)	missense	possibly damaging	0.86
Z1088:Muc5ac	UTSW	7	141,363,481 (GRCm39)	intron	probably benign
Z1177:Muc5ac	UTSW	7	141,371,777 (GRCm39)	missense	probably benign	0.33
Z1177:Muc5ac	UTSW	7	141,362,961 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- AGGAATGTGACTCTGCGCTG -3'
(R):5'- AGTTCTTTCAAAAGGAGCCCGAG -3'

Sequencing Primer
(F):5'- CGATGATGGCTCCAGTCAGAC -3'
(R):5'- GAGCCCGAGACCACCATGTC -3'

Posted On

2021-03-08