Mutagenetix > Incidental Mutation

Incidental Mutation 'R8771:Vmn2r61'

664409

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r61

Ensembl Gene

ENSMUSG00000090967

Gene Name

vomeronasal 2, receptor 61

Synonyms

Gprc2a-rs2, Casr-rs2, EG637873

MMRRC Submission

068602-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R8771 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

41909477-41950179 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 41916194 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Aspartic acid at position 269 (A269D)

Ref Sequence

ENSEMBL: ENSMUSP00000129576 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166131]

AlphaFold

L7N2B8

Predicted Effect

probably damaging
Transcript: ENSMUST00000166131
AA Change: A269D

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000129576
Gene: ENSMUSG00000090967
AA Change: A269D

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	4e-42	PFAM
Pfam:NCD3G	514	567	1.9e-21	PFAM
Pfam:7tm_3	600	835	6.2e-52	PFAM

Meta Mutation Damage Score

0.6329

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

100% (47/47)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	T	C	3: 121,880,320 (GRCm39)	I451T	probably damaging	Het
Acin1	T	C	14: 54,880,496 (GRCm39)	K1236E	unknown	Het
AI661453	T	C	17: 47,777,683 (GRCm39)	S470P	unknown	Het
Ano5	C	A	7: 51,216,095 (GRCm39)	N357K	probably damaging	Het
Ano5	C	T	7: 51,220,047 (GRCm39)	Q396*	probably null	Het
Baz1b	T	A	5: 135,273,151 (GRCm39)	M1425K	probably benign	Het
Cdh22	A	T	2: 164,988,689 (GRCm39)	V222E	possibly damaging	Het
Celsr1	A	T	15: 85,788,175 (GRCm39)	S2692R	probably benign	Het
Ces5a	A	G	8: 94,255,249 (GRCm39)	I144T	possibly damaging	Het
D5Ertd579e	T	A	5: 36,761,940 (GRCm39)	N1309I	probably damaging	Het
Dnmt3b	C	T	2: 153,504,734 (GRCm39)	T112M	possibly damaging	Het
Eif4g3	C	T	4: 137,907,848 (GRCm39)	Q1283*	probably null	Het
Etnppl	T	A	3: 130,414,024 (GRCm39)	M41K	probably damaging	Het
Hhatl	G	A	9: 121,617,776 (GRCm39)	T271I	possibly damaging	Het
Igkv5-37	A	G	6: 69,940,423 (GRCm39)	S74P	probably damaging	Het
Krt87	A	G	15: 101,385,779 (GRCm39)	V272A	probably benign	Het
Lgr6	A	T	1: 134,933,429 (GRCm39)	L262*	probably null	Het
Lrriq3	G	A	3: 154,899,270 (GRCm39)	V590I	probably damaging	Het
Mfsd13a	T	C	19: 46,360,668 (GRCm39)	V382A	probably damaging	Het
Minar2	T	C	18: 59,200,052 (GRCm39)		probably benign	Het
Mrc2	A	G	11: 105,240,596 (GRCm39)	T1429A	probably benign	Het
Mroh5	A	T	15: 73,693,203 (GRCm39)	M79K	possibly damaging	Het
Myo1c	A	G	11: 75,556,709 (GRCm39)	K553R	probably benign	Het
Nbeal1	A	G	1: 60,300,743 (GRCm39)	T1488A	probably benign	Het
Or4c12	T	C	2: 89,773,565 (GRCm39)	K298R	probably benign	Het
Or5aq6	T	C	2: 86,923,294 (GRCm39)	Y149C	probably benign	Het
Or5m10b	T	G	2: 85,699,712 (GRCm39)	Y259D	probably damaging	Het
Or5p76	T	A	7: 108,122,632 (GRCm39)	N175I	possibly damaging	Het
Prl7a1	A	T	13: 27,819,811 (GRCm39)	W148R	probably damaging	Het
Ptpn2	G	T	18: 67,805,659 (GRCm39)	T402K	probably benign	Het
Ptprf	A	G	4: 118,068,987 (GRCm39)	M1665T	possibly damaging	Het
Raet1e	G	T	10: 22,057,041 (GRCm39)	V122F	probably damaging	Het
Serpina3m	A	G	12: 104,357,841 (GRCm39)	E255G	probably damaging	Het
Sorcs2	T	C	5: 36,188,624 (GRCm39)	Y828C	probably damaging	Het
Sprr1a	T	A	3: 92,391,989 (GRCm39)	H4L	probably benign	Het
Sptssa	A	G	12: 54,703,211 (GRCm39)	Y20H	probably damaging	Het
Synpo2l	A	G	14: 20,710,491 (GRCm39)	S939P	probably damaging	Het
Tdrd7	T	C	4: 46,010,800 (GRCm39)	V568A	probably damaging	Het
Tmem132c	C	T	5: 127,437,192 (GRCm39)	P227L	probably benign	Het
Tmtc3	A	G	10: 100,286,180 (GRCm39)	S548P	possibly damaging	Het
Trip12	A	T	1: 84,721,018 (GRCm39)		probably benign	Het
Ttll6	A	G	11: 96,042,762 (GRCm39)	Y436C	probably damaging	Het
Unc80	A	G	1: 66,685,554 (GRCm39)	D2226G	possibly damaging	Het
Vmn1r142	T	C	7: 21,862,737 (GRCm39)	M242V	probably benign	Het
Vmn2r12	T	G	5: 109,239,952 (GRCm39)	T204P	possibly damaging	Het
Vmn2r16	C	T	5: 109,488,231 (GRCm39)	T368M	probably benign	Het
Zfp148	A	G	16: 33,317,656 (GRCm39)	D776G	possibly damaging	Het
Zfp775	A	T	6: 48,596,906 (GRCm39)	Q260L	probably benign	Het

Other mutations in Vmn2r61

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r61	APN	7	41,950,175 (GRCm39)	missense	possibly damaging	0.96
IGL00824:Vmn2r61	APN	7	41,916,438 (GRCm39)	missense	probably benign	0.03
IGL00903:Vmn2r61	APN	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
IGL01125:Vmn2r61	APN	7	41,909,550 (GRCm39)	missense	probably damaging	1.00
IGL01393:Vmn2r61	APN	7	41,916,258 (GRCm39)	missense	probably benign	0.08
IGL01712:Vmn2r61	APN	7	41,909,661 (GRCm39)	missense	probably damaging	0.98
IGL01822:Vmn2r61	APN	7	41,950,130 (GRCm39)	missense	probably benign	0.18
IGL01835:Vmn2r61	APN	7	41,950,015 (GRCm39)	missense	probably benign	0.12
IGL01844:Vmn2r61	APN	7	41,909,639 (GRCm39)	missense	probably benign	0.00
IGL01953:Vmn2r61	APN	7	41,949,613 (GRCm39)	missense	probably damaging	1.00
IGL02032:Vmn2r61	APN	7	41,949,466 (GRCm39)	missense	probably damaging	0.99
IGL02054:Vmn2r61	APN	7	41,926,158 (GRCm39)	critical splice donor site	probably null
IGL02569:Vmn2r61	APN	7	41,926,070 (GRCm39)	missense	probably damaging	1.00
IGL02697:Vmn2r61	APN	7	41,924,892 (GRCm39)	missense	possibly damaging	0.55
IGL02958:Vmn2r61	APN	7	41,949,361 (GRCm39)	missense	probably benign
IGL03290:Vmn2r61	APN	7	41,915,408 (GRCm39)	missense	probably benign	0.00
IGL03337:Vmn2r61	APN	7	41,916,509 (GRCm39)	missense	possibly damaging	0.58
IGL03369:Vmn2r61	APN	7	41,909,517 (GRCm39)	missense	probably benign
IGL03402:Vmn2r61	APN	7	41,909,679 (GRCm39)	missense	probably benign
R0026:Vmn2r61	UTSW	7	41,924,898 (GRCm39)	missense	possibly damaging	0.64
R0319:Vmn2r61	UTSW	7	41,949,941 (GRCm39)	missense	probably damaging	0.99
R0433:Vmn2r61	UTSW	7	41,915,335 (GRCm39)	missense	probably benign	0.02
R0555:Vmn2r61	UTSW	7	41,915,442 (GRCm39)	missense	probably benign	0.02
R0691:Vmn2r61	UTSW	7	41,949,844 (GRCm39)	missense	probably damaging	1.00
R1701:Vmn2r61	UTSW	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
R1718:Vmn2r61	UTSW	7	41,950,121 (GRCm39)	missense	probably benign
R1835:Vmn2r61	UTSW	7	41,916,076 (GRCm39)	nonsense	probably null
R1920:Vmn2r61	UTSW	7	41,949,710 (GRCm39)	missense	possibly damaging	0.73
R2069:Vmn2r61	UTSW	7	41,949,425 (GRCm39)	missense	probably benign	0.06
R2326:Vmn2r61	UTSW	7	41,916,287 (GRCm39)	missense	probably damaging	1.00
R2402:Vmn2r61	UTSW	7	41,949,529 (GRCm39)	missense	possibly damaging	0.90
R3103:Vmn2r61	UTSW	7	41,916,067 (GRCm39)	missense	possibly damaging	0.73
R3107:Vmn2r61	UTSW	7	41,916,491 (GRCm39)	missense	possibly damaging	0.82
R4426:Vmn2r61	UTSW	7	41,950,159 (GRCm39)	missense	probably benign
R4426:Vmn2r61	UTSW	7	41,950,157 (GRCm39)	missense	probably benign
R4484:Vmn2r61	UTSW	7	41,950,120 (GRCm39)	missense	probably benign
R4748:Vmn2r61	UTSW	7	41,916,565 (GRCm39)	missense	probably damaging	0.96
R4835:Vmn2r61	UTSW	7	41,916,459 (GRCm39)	missense	possibly damaging	0.52
R4863:Vmn2r61	UTSW	7	41,950,132 (GRCm39)	missense	probably benign	0.03
R4923:Vmn2r61	UTSW	7	41,916,520 (GRCm39)	missense	probably damaging	1.00
R4968:Vmn2r61	UTSW	7	41,949,478 (GRCm39)	missense	probably benign	0.14
R5114:Vmn2r61	UTSW	7	41,949,953 (GRCm39)	missense	possibly damaging	0.92
R5297:Vmn2r61	UTSW	7	41,909,646 (GRCm39)	missense	probably benign
R5497:Vmn2r61	UTSW	7	41,924,906 (GRCm39)	missense	possibly damaging	0.95
R5508:Vmn2r61	UTSW	7	41,916,242 (GRCm39)	missense	possibly damaging	0.52
R5587:Vmn2r61	UTSW	7	41,949,911 (GRCm39)	missense	probably damaging	1.00
R5615:Vmn2r61	UTSW	7	41,949,917 (GRCm39)	missense	probably damaging	1.00
R5615:Vmn2r61	UTSW	7	41,916,677 (GRCm39)	missense	probably benign	0.00
R5782:Vmn2r61	UTSW	7	41,949,253 (GRCm39)	missense	probably damaging	1.00
R6136:Vmn2r61	UTSW	7	41,916,455 (GRCm39)	missense	probably damaging	1.00
R6207:Vmn2r61	UTSW	7	41,909,616 (GRCm39)	missense	probably benign	0.01
R6265:Vmn2r61	UTSW	7	41,915,915 (GRCm39)	missense	probably benign	0.01
R6272:Vmn2r61	UTSW	7	41,949,242 (GRCm39)	missense	probably damaging	1.00
R6355:Vmn2r61	UTSW	7	41,916,659 (GRCm39)	missense	probably benign	0.00
R6469:Vmn2r61	UTSW	7	41,915,283 (GRCm39)	nonsense	probably null
R6554:Vmn2r61	UTSW	7	41,926,139 (GRCm39)	missense	probably damaging	1.00
R6699:Vmn2r61	UTSW	7	41,949,580 (GRCm39)	missense	probably benign
R6768:Vmn2r61	UTSW	7	41,949,748 (GRCm39)	missense	probably damaging	1.00
R6824:Vmn2r61	UTSW	7	41,949,403 (GRCm39)	missense	probably benign	0.10
R6930:Vmn2r61	UTSW	7	41,949,364 (GRCm39)	missense	probably benign	0.02
R7053:Vmn2r61	UTSW	7	41,916,557 (GRCm39)	missense	probably damaging	0.96
R7238:Vmn2r61	UTSW	7	41,916,629 (GRCm39)	missense	possibly damaging	0.73
R7332:Vmn2r61	UTSW	7	41,909,534 (GRCm39)	missense	probably benign	0.00
R7359:Vmn2r61	UTSW	7	41,915,407 (GRCm39)	missense	probably benign	0.11
R7553:Vmn2r61	UTSW	7	41,916,205 (GRCm39)	missense	not run
R7710:Vmn2r61	UTSW	7	41,916,472 (GRCm39)	missense	probably damaging	1.00
R7732:Vmn2r61	UTSW	7	41,916,097 (GRCm39)	missense	probably benign
R7839:Vmn2r61	UTSW	7	41,916,032 (GRCm39)	missense	probably damaging	0.97
R7916:Vmn2r61	UTSW	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
R8026:Vmn2r61	UTSW	7	41,916,141 (GRCm39)	missense	probably benign	0.02
R8440:Vmn2r61	UTSW	7	41,916,080 (GRCm39)	missense	probably benign	0.02
R8499:Vmn2r61	UTSW	7	41,949,700 (GRCm39)	missense	probably damaging	0.99
R8847:Vmn2r61	UTSW	7	41,950,010 (GRCm39)	missense	probably damaging	1.00
R8986:Vmn2r61	UTSW	7	41,915,325 (GRCm39)	nonsense	probably null
R9290:Vmn2r61	UTSW	7	41,915,385 (GRCm39)	missense	probably benign	0.27
R9311:Vmn2r61	UTSW	7	41,950,092 (GRCm39)	missense	possibly damaging	0.92
R9324:Vmn2r61	UTSW	7	41,916,619 (GRCm39)	missense	probably benign	0.00
R9476:Vmn2r61	UTSW	7	41,949,593 (GRCm39)	missense	probably damaging	1.00
R9521:Vmn2r61	UTSW	7	41,916,626 (GRCm39)	missense	probably damaging	0.99
R9619:Vmn2r61	UTSW	7	41,926,136 (GRCm39)	missense	probably damaging	0.98
R9729:Vmn2r61	UTSW	7	41,949,917 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r61	UTSW	7	41,949,388 (GRCm39)	missense	possibly damaging	0.93
Z1176:Vmn2r61	UTSW	7	41,916,166 (GRCm39)	missense	probably benign	0.00
Z1176:Vmn2r61	UTSW	7	41,909,585 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- ACTGGGTTGGTCTGATCCTC -3'
(R):5'- TTAGGAAGATAATTGTCCTCTGGG -3'

Sequencing Primer
(F):5'- TCCCAGATGATCACAGAGGGATTC -3'
(R):5'- CCTCTGGGTATTTGTAGGGATTAAC -3'

Posted On

2021-03-08