Mutagenetix > Incidental Mutation

Incidental Mutation 'R8475:Oxnad1'

668180

Institutional Source

Beutler Lab

Gene Symbol

Oxnad1

Ensembl Gene

ENSMUSG00000021906

Gene Name

oxidoreductase NAD-binding domain containing 1

Synonyms

2410002F01Rik

MMRRC Submission

067919-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.071) question?

Stock #

R8475 (G1)

Quality Score

96.0077

Status

Validated

Chromosome

Chromosomal Location

31807331-31825159 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to G at 31823250 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022462] [ENSMUST00000164868] [ENSMUST00000165523] [ENSMUST00000168986] [ENSMUST00000169649] [ENSMUST00000170600] [ENSMUST00000171598]

AlphaFold

Q8VE38

Predicted Effect

probably benign
Transcript: ENSMUST00000022462

SMART Domains

Protein: ENSMUSP00000022462
Gene: ENSMUSG00000021906

Domain	Start	End	E-Value	Type
Pfam:NAD_binding_6	168	269	6.5e-9	PFAM
Pfam:NAD_binding_1	173	293	8.1e-16	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000164868

SMART Domains

Protein: ENSMUSP00000125791
Gene: ENSMUSG00000021906

Domain	Start	End	E-Value	Type
SCOP:d1ep3b1	65	149	1e-11	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000165523

Predicted Effect

probably benign
Transcript: ENSMUST00000168986

SMART Domains

Protein: ENSMUSP00000126372
Gene: ENSMUSG00000021906

Domain	Start	End	E-Value	Type
SCOP:d1ep3b1	65	130	3e-10	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000169649

Predicted Effect

probably benign
Transcript: ENSMUST00000170519

SMART Domains

Protein: ENSMUSP00000132560
Gene: ENSMUSG00000021906

Domain	Start	End	E-Value	Type
SCOP:d1ep3b1	2	78	8e-9	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000170600

SMART Domains

Protein: ENSMUSP00000130998
Gene: ENSMUSG00000021906

Domain	Start	End	E-Value	Type
SCOP:d1ep3b1	65	143	3e-10	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000171598

SMART Domains

Protein: ENSMUSP00000132162
Gene: ENSMUSG00000021906

Domain	Start	End	E-Value	Type
SCOP:d1ep3b1	65	115	4e-8	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000171849

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (48/48)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700123K08Rik	G	A	5: 138,561,188 (GRCm39)	T158M	probably benign	Het
Adamts14	T	C	10: 61,038,666 (GRCm39)	E945G	probably damaging	Het
Adgrl3	T	C	5: 81,871,976 (GRCm39)	V958A	probably benign	Het
Adh6a	T	C	3: 138,030,979 (GRCm39)	I156T	probably benign	Het
Akt1	A	G	12: 112,624,863 (GRCm39)	V201A	possibly damaging	Het
Als2cl	C	G	9: 110,715,484 (GRCm39)	A182G	possibly damaging	Het
Amn	T	A	12: 111,241,819 (GRCm39)	L295Q	probably benign	Het
Dbf4	A	G	5: 8,448,664 (GRCm39)		probably benign	Het
Depdc7	G	A	2: 104,552,314 (GRCm39)	R498C	probably benign	Het
Eogt	A	T	6: 97,122,327 (GRCm39)	Y35*	probably null	Het
Epb41l1	T	G	2: 156,364,150 (GRCm39)	S886R	probably damaging	Het
Fshr	A	T	17: 89,293,456 (GRCm39)	D407E	probably damaging	Het
Gngt1	C	A	6: 3,994,262 (GRCm39)	D13E	probably benign	Het
Grxcr1	T	C	5: 68,323,484 (GRCm39)	L254P	possibly damaging	Het
Itga11	A	G	9: 62,651,327 (GRCm39)	D290G	probably damaging	Het
Klf16	A	G	10: 80,412,719 (GRCm39)	S106P	probably benign	Het
Klk1b5	A	G	7: 43,500,204 (GRCm39)	D264G	possibly damaging	Het
Lama2	C	T	10: 26,977,369 (GRCm39)	V1937M	possibly damaging	Het
Lipi	T	A	16: 75,370,862 (GRCm39)	K118N	probably benign	Het
Manba	G	A	3: 135,217,573 (GRCm39)	V130I	probably benign	Het
Map2	A	G	1: 66,453,164 (GRCm39)	S527G	probably damaging	Het
Masp1	C	A	16: 23,271,281 (GRCm39)	V656L	probably damaging	Het
Mrpl10	A	T	11: 96,938,261 (GRCm39)	N137I	probably benign	Het
Mtfmt	C	A	9: 65,359,469 (GRCm39)	N381K	probably benign	Het
Myo18b	A	T	5: 113,021,422 (GRCm39)	Y546*	probably null	Het
Nectin4	C	T	1: 171,212,280 (GRCm39)	R293*	probably null	Het
Nudt3	A	G	17: 27,799,776 (GRCm39)	S136P	possibly damaging	Het
Or14c39	A	C	7: 86,344,361 (GRCm39)	R232S	probably benign	Het
Or1q1	G	A	2: 36,887,066 (GRCm39)	M81I	probably damaging	Het
Or4a81	T	C	2: 89,619,586 (GRCm39)	M37V	probably benign	Het
Or52h7	A	G	7: 104,214,066 (GRCm39)	M213V	probably benign	Het
Or9m2	T	C	2: 87,820,536 (GRCm39)	L27P	probably damaging	Het
Parn	A	G	16: 13,425,113 (GRCm39)		probably null	Het
Pcdha12	T	C	18: 37,154,136 (GRCm39)	V285A	possibly damaging	Het
Pfkfb2	G	C	1: 130,624,816 (GRCm39)	Q515E	probably benign	Het
Pjvk	T	C	2: 76,480,901 (GRCm39)	F40L	probably benign	Het
Pygo2	A	G	3: 89,340,697 (GRCm39)	Y328C	probably damaging	Het
Rapgef5	A	C	12: 117,681,965 (GRCm39)	K215Q	probably damaging	Het
Sephs1	T	A	2: 4,893,821 (GRCm39)		probably null	Het
Slc12a9	C	A	5: 137,313,737 (GRCm39)	V741L	probably benign	Het
Spmip4	A	G	6: 50,566,107 (GRCm39)	Y123H	probably damaging	Het
Tbx21	A	T	11: 96,990,808 (GRCm39)	V290D	possibly damaging	Het
Tep1	C	A	14: 51,078,712 (GRCm39)	G1514W	probably damaging	Het
Vmn2r16	T	C	5: 109,487,073 (GRCm39)	M98T	probably benign	Het
Zfr	A	G	15: 12,150,455 (GRCm39)	N474S	probably benign	Het
Zpbp	T	C	11: 11,365,203 (GRCm39)	K215E	possibly damaging	Het

Other mutations in Oxnad1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0480:Oxnad1	UTSW	14	31,821,437 (GRCm39)	missense	probably damaging	1.00
R0879:Oxnad1	UTSW	14	31,821,553 (GRCm39)	missense	probably damaging	1.00
R1482:Oxnad1	UTSW	14	31,821,590 (GRCm39)	critical splice donor site	probably null
R1526:Oxnad1	UTSW	14	31,824,244 (GRCm39)	missense	probably benign	0.00
R4649:Oxnad1	UTSW	14	31,824,366 (GRCm39)	makesense	probably null
R4703:Oxnad1	UTSW	14	31,817,427 (GRCm39)	missense	probably damaging	1.00
R6411:Oxnad1	UTSW	14	31,813,609 (GRCm39)	missense	possibly damaging	0.92
R6980:Oxnad1	UTSW	14	31,807,576 (GRCm39)	unclassified	probably benign
R7117:Oxnad1	UTSW	14	31,813,608 (GRCm39)	missense	probably benign	0.00
R7167:Oxnad1	UTSW	14	31,822,976 (GRCm39)	nonsense	probably null
R7197:Oxnad1	UTSW	14	31,818,473 (GRCm39)	missense	possibly damaging	0.50
R7707:Oxnad1	UTSW	14	31,823,965 (GRCm39)	splice site	probably null
R7809:Oxnad1	UTSW	14	31,822,962 (GRCm39)	missense	probably benign
R8139:Oxnad1	UTSW	14	31,814,048 (GRCm39)	missense	possibly damaging	0.68
R8421:Oxnad1	UTSW	14	31,821,431 (GRCm39)	missense	probably benign	0.05
X0020:Oxnad1	UTSW	14	31,813,718 (GRCm39)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- TAACTAGCTGCACAGTTACTGG -3'
(R):5'- GCCCTTGGTATTCCTACAGACC -3'

Sequencing Primer
(F):5'- CTAGCTGCACAGTTACTGGATAGGC -3'
(R):5'- GGATCTTCATGAGTTCCAGACTAGC -3'

Posted On

2021-03-19