Mutagenetix > Incidental Mutation

Incidental Mutation 'R8491:Zfp958'

668200

Institutional Source

Beutler Lab

Gene Symbol

Zfp958

Ensembl Gene

ENSMUSG00000058748

Gene Name

zinc finger protein 958

Synonyms

BC003267

MMRRC Submission

067933-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R8491 (G1)

Quality Score

57.0073

Status

Validated

Chromosome

Chromosomal Location

4663167-4680231 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 4676215 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Isoleucine at position 61 (R61I)

Ref Sequence

ENSEMBL: ENSMUSP00000143842 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073201] [ENSMUST00000202692]

AlphaFold

Q99LG7

Predicted Effect

probably damaging
Transcript: ENSMUST00000073201
AA Change: R60I

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000072937
Gene: ENSMUSG00000058748
AA Change: R60I

Domain	Start	End	E-Value	Type
KRAB	3	65	1.96e-17	SMART
ZnF_C2H2	102	124	2.67e-1	SMART
ZnF_C2H2	130	152	8.47e-4	SMART
ZnF_C2H2	158	180	5.5e-3	SMART
ZnF_C2H2	186	208	1.38e-3	SMART
ZnF_C2H2	214	236	9.96e-1	SMART
ZnF_C2H2	242	264	2.15e-5	SMART
ZnF_C2H2	270	292	3.16e-3	SMART
ZnF_C2H2	298	320	1.69e-3	SMART
ZnF_C2H2	326	348	5.14e-3	SMART
ZnF_C2H2	354	376	2.91e-2	SMART
ZnF_C2H2	382	404	2.27e-4	SMART
ZnF_C2H2	410	432	4.79e-3	SMART
ZnF_C2H2	438	460	6.88e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000202692
AA Change: R61I

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000143842
Gene: ENSMUSG00000058748
AA Change: R61I

Domain	Start	End	E-Value	Type
KRAB	4	66	1.96e-17	SMART
ZnF_C2H2	103	125	2.67e-1	SMART
ZnF_C2H2	131	153	8.47e-4	SMART
ZnF_C2H2	159	181	5.5e-3	SMART
ZnF_C2H2	187	209	1.38e-3	SMART
ZnF_C2H2	215	237	9.96e-1	SMART
ZnF_C2H2	243	265	2.15e-5	SMART
ZnF_C2H2	271	293	3.16e-3	SMART
ZnF_C2H2	299	321	1.69e-3	SMART
ZnF_C2H2	327	349	5.14e-3	SMART
ZnF_C2H2	355	377	2.91e-2	SMART
ZnF_C2H2	383	405	2.27e-4	SMART
ZnF_C2H2	411	433	4.79e-3	SMART
ZnF_C2H2	439	461	6.88e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (52/52)

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aard	A	T	15: 51,903,708 (GRCm39)	I44F	unknown	Het
Agap1	A	G	1: 89,537,294 (GRCm39)	E100G	probably damaging	Het
Aoc3	G	T	11: 101,223,042 (GRCm39)	R426L	probably benign	Het
Aspm	T	G	1: 139,385,433 (GRCm39)	L359R	probably damaging	Het
Atp8b2	A	G	3: 89,865,676 (GRCm39)	S75P	probably damaging	Het
Bcl9l	A	G	9: 44,412,065 (GRCm39)	E17G	probably benign	Het
Casc3	G	T	11: 98,713,977 (GRCm39)	R403L	probably benign	Het
Cdh16	T	C	8: 105,343,681 (GRCm39)	D605G	probably damaging	Het
Cdh2	A	G	18: 16,757,775 (GRCm39)		probably null	Het
Cers5	T	A	15: 99,638,831 (GRCm39)	K161N	probably damaging	Het
Cfap57	C	T	4: 118,472,128 (GRCm39)	V84I	probably benign	Het
Cntnap3	A	T	13: 64,933,157 (GRCm39)	D454E	probably damaging	Het
Cxcl15	T	A	5: 90,943,089 (GRCm39)	C30*	probably null	Het
Cyp4a12a	C	A	4: 115,158,650 (GRCm39)		probably null	Het
Dlc1	A	T	8: 37,052,000 (GRCm39)	I577N	probably benign	Het
Dusp6	C	A	10: 99,102,081 (GRCm39)	R210S	possibly damaging	Het
Faxc	T	A	4: 21,993,319 (GRCm39)	M321K	probably damaging	Het
Fez2	C	A	17: 78,692,200 (GRCm39)	V340L	probably benign	Het
G530012D18Rik	C	G	1: 85,504,935 (GRCm39)	D113E	unknown	Het
Gdnf	A	G	15: 7,864,272 (GRCm39)	I228V	possibly damaging	Het
Gm12258	A	G	11: 58,745,122 (GRCm39)	T18A		Het
Gm5460	T	C	14: 33,761,740 (GRCm39)	L194P	probably damaging	Het
Gm6040	T	G	8: 21,407,135 (GRCm39)	R28S	possibly damaging	Het
Gmps	A	G	3: 63,921,779 (GRCm39)	E594G	probably benign	Het
Hspg2	T	C	4: 137,281,030 (GRCm39)	V3334A	probably benign	Het
Idh3a	T	C	9: 54,506,963 (GRCm39)		probably null	Het
Ins1	A	G	19: 52,252,808 (GRCm39)		probably benign	Het
Mug1	A	T	6: 121,859,688 (GRCm39)	D1229V	probably damaging	Het
Ndufs1	T	C	1: 63,196,384 (GRCm39)	D347G	probably damaging	Het
Or52d3	G	A	7: 104,229,242 (GRCm39)	V130I	probably damaging	Het
Or8k25	A	G	2: 86,244,099 (GRCm39)	I99T	probably benign	Het
Pclo	C	A	5: 14,565,244 (GRCm39)	N3K	unknown	Het
Pigl	A	G	11: 62,364,293 (GRCm39)	R112G	probably null	Het
Prkcq	T	C	2: 11,284,335 (GRCm39)	Y502H	probably damaging	Het
Psg16	T	C	7: 16,824,437 (GRCm39)	Y74H	probably damaging	Het
Psme4	G	A	11: 30,722,161 (GRCm39)	G60D	possibly damaging	Het
Rpp14	A	T	14: 8,083,925 (GRCm38)	Q27L	possibly damaging	Het
Serpini2	A	C	3: 75,159,822 (GRCm39)	C315G	probably damaging	Het
Slitrk3	A	G	3: 72,958,592 (GRCm39)	I60T	possibly damaging	Het
Svil	T	C	18: 5,106,678 (GRCm39)	Y1436H	probably damaging	Het
Tmem245	T	C	4: 56,906,261 (GRCm39)	Q548R	probably benign	Het
Tob1	A	G	11: 94,105,115 (GRCm39)	D217G	probably benign	Het
Trim21	A	C	7: 102,208,689 (GRCm39)	D343E	probably benign	Het
Triobp	C	T	15: 78,878,326 (GRCm39)	H1750Y	possibly damaging	Het
Trmt13	T	C	3: 116,376,228 (GRCm39)	R388G	probably benign	Het
Ubqlnl	A	G	7: 103,798,582 (GRCm39)	V305A	probably benign	Het
Ushbp1	A	G	8: 71,845,041 (GRCm39)	V244A	probably benign	Het
Vdac2	T	A	14: 21,887,838 (GRCm39)	N60K	possibly damaging	Het
Vwa5a	A	G	9: 38,652,476 (GRCm39)	E753G	probably damaging	Het
Washc4	A	G	10: 83,411,987 (GRCm39)	D706G	probably benign	Het
Zbtb5	T	C	4: 44,995,090 (GRCm39)	D98G	probably damaging	Het
Zfp53	A	G	17: 21,729,621 (GRCm39)	I551M	probably benign	Het

Other mutations in Zfp958

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01950:Zfp958	APN	8	4,678,917 (GRCm39)	missense	probably damaging	1.00
IGL02185:Zfp958	APN	8	4,678,990 (GRCm39)	nonsense	probably null
IGL02716:Zfp958	APN	8	4,675,967 (GRCm39)	critical splice donor site	probably null
R1118:Zfp958	UTSW	8	4,676,169 (GRCm39)	missense	possibly damaging	0.50
R1119:Zfp958	UTSW	8	4,676,169 (GRCm39)	missense	possibly damaging	0.50
R1478:Zfp958	UTSW	8	4,679,190 (GRCm39)	missense	probably damaging	1.00
R1816:Zfp958	UTSW	8	4,679,147 (GRCm39)	missense	possibly damaging	0.89
R1837:Zfp958	UTSW	8	4,678,590 (GRCm39)	missense	probably damaging	1.00
R1838:Zfp958	UTSW	8	4,678,590 (GRCm39)	missense	probably damaging	1.00
R3763:Zfp958	UTSW	8	4,676,226 (GRCm39)	critical splice donor site	probably null
R5257:Zfp958	UTSW	8	4,678,456 (GRCm39)	missense	probably benign	0.28
R5258:Zfp958	UTSW	8	4,678,456 (GRCm39)	missense	probably benign	0.28
R5304:Zfp958	UTSW	8	4,676,196 (GRCm39)	missense	possibly damaging	0.73
R5512:Zfp958	UTSW	8	4,675,838 (GRCm39)	critical splice acceptor site	probably null
R5907:Zfp958	UTSW	8	4,679,072 (GRCm39)	missense	probably benign	0.01
R6604:Zfp958	UTSW	8	4,678,245 (GRCm39)	missense	probably damaging	1.00
R6688:Zfp958	UTSW	8	4,678,940 (GRCm39)	missense	possibly damaging	0.91
R6727:Zfp958	UTSW	8	4,678,247 (GRCm39)	missense	probably benign	0.08
R6981:Zfp958	UTSW	8	4,676,170 (GRCm39)	missense	probably benign	0.00
R7010:Zfp958	UTSW	8	4,678,377 (GRCm39)	missense	probably benign	0.13
R7140:Zfp958	UTSW	8	4,678,481 (GRCm39)	missense	probably benign	0.05
R8096:Zfp958	UTSW	8	4,663,273 (GRCm39)	start gained	probably benign
R8673:Zfp958	UTSW	8	4,678,268 (GRCm39)	missense	possibly damaging	0.54
R8847:Zfp958	UTSW	8	4,678,434 (GRCm39)	missense	probably damaging	1.00
R9612:Zfp958	UTSW	8	4,678,298 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCTTTGATGATGTGCATGTGGA -3'
(R):5'- CTCTGAACCTGTAAGCCAACCC -3'

Sequencing Primer
(F):5'- TACTGGAACCTCACTGCTATAGG -3'
(R):5'- AAGTTGTCCTTATAAGAGTTGCCTTG -3'

Posted On

2021-04-21