Mutagenetix > Incidental Mutation

Incidental Mutation 'R8696:Vmn1r225'

668740

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r225

Ensembl Gene

ENSMUSG00000043537

Gene Name

vomeronasal 1 receptor 225

Synonyms

V1re5

MMRRC Submission

068550-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R8696 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

20722561-20723457 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 20723419 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Cysteine at position 287 (S287C)

Ref Sequence

ENSEMBL: ENSMUSP00000056068 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061660]

AlphaFold

Q8R2A5

Predicted Effect

probably damaging
Transcript: ENSMUST00000061660
AA Change: S287C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000056068
Gene: ENSMUSG00000043537
AA Change: S287C

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	287	8.9e-15	PFAM
Pfam:V1R	11	291	3.1e-24	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy8	T	C	15: 64,687,235 (GRCm39)	N423S	probably benign	Het
Adgrl4	T	C	3: 151,248,344 (GRCm39)	L672P	probably damaging	Het
Akr1b10	C	T	6: 34,369,067 (GRCm39)	T182I	possibly damaging	Het
Ankrd33	A	G	15: 101,014,864 (GRCm39)	I84V	probably benign	Het
Arhgap32	A	G	9: 32,159,799 (GRCm39)	Y272C	possibly damaging	Het
Arid4a	T	C	12: 71,110,090 (GRCm39)	S144P	probably damaging	Het
Bicd1	A	G	6: 149,415,285 (GRCm39)	D666G	probably damaging	Het
Blk	A	T	14: 63,618,149 (GRCm39)		probably benign	Het
Btbd2	C	T	10: 80,480,515 (GRCm39)	R366Q	possibly damaging	Het
Cacna1i	T	C	15: 80,266,175 (GRCm39)	V1554A	probably damaging	Het
Camsap3	T	C	8: 3,653,614 (GRCm39)	L428P	probably damaging	Het
Ccdc30	T	G	4: 119,234,505 (GRCm39)	Y69S	possibly damaging	Het
Ccdc7a	T	A	8: 129,715,244 (GRCm39)	E280V	probably damaging	Het
Cdk11b	T	C	4: 155,732,779 (GRCm39)	V539A	unknown	Het
Ceacam3	A	T	7: 16,893,937 (GRCm39)	N483Y		Het
Cenpf	C	T	1: 189,390,194 (GRCm39)	A1213T	probably benign	Het
Chrna6	G	T	8: 27,897,195 (GRCm39)	Y227*	probably null	Het
Cntnap5c	A	T	17: 58,601,294 (GRCm39)	D814V	probably damaging	Het
Csl	A	T	10: 99,594,826 (GRCm39)	Y80N	probably damaging	Het
Ctsm	A	G	13: 61,685,521 (GRCm39)	L297P	probably damaging	Het
Cyp4a31	G	A	4: 115,422,225 (GRCm39)	E70K	probably benign	Het
Dhx8	T	C	11: 101,623,958 (GRCm39)	V31A	unknown	Het
Dis3l2	G	T	1: 86,719,162 (GRCm39)	G131*	probably null	Het
Dkk1	G	A	19: 30,526,688 (GRCm39)	A31V	probably damaging	Het
Dlx3	C	T	11: 95,012,596 (GRCm39)	R130*	probably null	Het
Dnajc24	T	C	2: 105,832,315 (GRCm39)	M23V	probably benign	Het
Dnmbp	T	A	19: 43,862,662 (GRCm39)	N76I	probably damaging	Het
Ezh1	T	C	11: 101,100,305 (GRCm39)	N226S	probably benign	Het
Fabp9	C	T	3: 10,259,047 (GRCm39)	V120M	possibly damaging	Het
Fcrlb	A	G	1: 170,739,648 (GRCm39)	C85R	probably damaging	Het
Gnai2	A	C	9: 107,496,968 (GRCm39)	L131R		Het
Gprin1	A	G	13: 54,885,764 (GRCm39)	W837R	probably damaging	Het
Grm3	C	T	5: 9,562,311 (GRCm39)	C513Y	probably damaging	Het
H60c	T	A	10: 3,210,265 (GRCm39)	I95F	possibly damaging	Het
Hecw1	A	G	13: 14,531,743 (GRCm39)	V177A	possibly damaging	Het
Herc6	T	A	6: 57,624,134 (GRCm39)	C635S	probably benign	Het
Itga4	A	T	2: 79,112,125 (GRCm39)	M347L	probably benign	Het
Kif20b	A	G	19: 34,914,752 (GRCm39)	H436R	probably benign	Het
Klrg2	G	T	6: 38,613,430 (GRCm39)	P191Q	possibly damaging	Het
Krt5	A	G	15: 101,618,742 (GRCm39)	Y340H	probably damaging	Het
Map9	T	A	3: 82,270,668 (GRCm39)	H77Q	possibly damaging	Het
Mfsd13a	A	G	19: 46,356,557 (GRCm39)	T221A	probably benign	Het
Myo10	C	G	15: 25,799,572 (GRCm39)	H1378Q	probably damaging	Het
Nkx6-1	T	C	5: 101,807,513 (GRCm39)	T290A	possibly damaging	Het
Nlgn3	T	C	X: 100,352,390 (GRCm39)	V179A	probably damaging	Het
Notch1	T	A	2: 26,368,004 (GRCm39)		probably benign	Het
Nr1d2	A	T	14: 18,216,661 (GRCm38)	M169K	probably damaging	Het
Or10ak8	C	T	4: 118,774,635 (GRCm39)	V10I	probably benign	Het
Or11g1	A	G	14: 50,651,420 (GRCm39)	M140V	possibly damaging	Het
Or13c7c	A	G	4: 43,836,193 (GRCm39)	V99A	probably benign	Het
Or6c69b	A	T	10: 129,626,562 (GRCm39)	F299I	possibly damaging	Het
Or8b41	A	G	9: 38,054,433 (GRCm39)	M1V	probably null	Het
Pdia6	T	A	12: 17,329,662 (GRCm39)	I266K	probably damaging	Het
Phactr4	T	C	4: 132,091,105 (GRCm39)		probably null	Het
Polr1b	T	A	2: 128,967,571 (GRCm39)	L988Q	probably damaging	Het
Por	A	T	5: 135,763,112 (GRCm39)	M541L	probably benign	Het
Ppid	T	C	3: 79,498,689 (GRCm39)		probably benign	Het
Prp2	A	T	6: 132,577,322 (GRCm39)	Q203L	unknown	Het
Psme4	T	G	11: 30,759,896 (GRCm39)	F340V	probably damaging	Het
Ptges2	T	C	2: 32,290,077 (GRCm39)	S164P	probably damaging	Het
Rab18	G	A	18: 6,788,635 (GRCm39)	G201S	probably damaging	Het
Rbm19	A	G	5: 120,265,132 (GRCm39)	E391G	probably damaging	Het
Rcbtb2	T	G	14: 73,404,305 (GRCm39)	V259G	probably damaging	Het
Rpn1	C	A	6: 88,080,359 (GRCm39)	Q553K	possibly damaging	Het
Sash1	A	G	10: 8,609,459 (GRCm39)	S697P	probably damaging	Het
Sdad1	T	G	5: 92,437,645 (GRCm39)	H603P	probably damaging	Het
Serpini1	T	C	3: 75,520,544 (GRCm39)	L47P	probably damaging	Het
Sorbs2	T	A	8: 46,248,686 (GRCm39)	S646T	possibly damaging	Het
Spdye4a	T	A	5: 143,210,754 (GRCm39)	E105D	probably benign	Het
Srsf12	G	A	4: 33,231,181 (GRCm39)	C230Y	possibly damaging	Het
Stard9	A	T	2: 120,531,595 (GRCm39)	R2617S	probably benign	Het
Tas2r118	G	T	6: 23,969,344 (GRCm39)	T239K	probably damaging	Het
Tlr1	A	G	5: 65,084,094 (GRCm39)	L161P	probably benign	Het
Ttc21a	T	G	9: 119,772,977 (GRCm39)	V218G	possibly damaging	Het
Ttc6	T	C	12: 57,784,492 (GRCm39)	S1854P	probably benign	Het
Unc93a	A	G	17: 13,341,852 (GRCm39)	L93P	probably damaging	Het
Vcan	A	T	13: 89,839,217 (GRCm39)	I2109N	probably benign	Het
Vmn1r43	A	G	6: 89,847,321 (GRCm39)	F55S	probably damaging	Het
Zfp772	T	C	7: 7,208,518 (GRCm39)	T109A	possibly damaging	Het
Zfp87	A	G	13: 74,520,599 (GRCm39)	Y160H	probably damaging	Het

Other mutations in Vmn1r225

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01093:Vmn1r225	APN	17	20,723,081 (GRCm39)	missense	probably damaging	1.00
IGL01830:Vmn1r225	APN	17	20,722,717 (GRCm39)	missense	probably damaging	0.96
IGL02943:Vmn1r225	APN	17	20,722,567 (GRCm39)	missense	possibly damaging	0.67
R0544:Vmn1r225	UTSW	17	20,722,718 (GRCm39)	missense	probably benign	0.44
R1126:Vmn1r225	UTSW	17	20,722,588 (GRCm39)	missense	probably benign	0.03
R1809:Vmn1r225	UTSW	17	20,722,918 (GRCm39)	missense	probably benign	0.04
R1928:Vmn1r225	UTSW	17	20,723,071 (GRCm39)	missense	probably benign	0.00
R2044:Vmn1r225	UTSW	17	20,722,852 (GRCm39)	missense	possibly damaging	0.50
R2191:Vmn1r225	UTSW	17	20,723,147 (GRCm39)	missense	probably damaging	0.98
R2206:Vmn1r225	UTSW	17	20,722,611 (GRCm39)	missense	possibly damaging	0.56
R2207:Vmn1r225	UTSW	17	20,722,611 (GRCm39)	missense	possibly damaging	0.56
R2680:Vmn1r225	UTSW	17	20,723,055 (GRCm39)	missense	probably benign	0.00
R3740:Vmn1r225	UTSW	17	20,723,261 (GRCm39)	missense	possibly damaging	0.56
R3807:Vmn1r225	UTSW	17	20,723,114 (GRCm39)	nonsense	probably null
R4196:Vmn1r225	UTSW	17	20,723,237 (GRCm39)	missense	probably benign	0.00
R4970:Vmn1r225	UTSW	17	20,722,831 (GRCm39)	missense	possibly damaging	0.74
R5129:Vmn1r225	UTSW	17	20,723,378 (GRCm39)	missense	probably damaging	1.00
R5130:Vmn1r225	UTSW	17	20,723,047 (GRCm39)	missense	possibly damaging	0.81
R5187:Vmn1r225	UTSW	17	20,723,177 (GRCm39)	missense	probably damaging	0.96
R5580:Vmn1r225	UTSW	17	20,723,101 (GRCm39)	missense	probably damaging	1.00
R6563:Vmn1r225	UTSW	17	20,722,763 (GRCm39)	missense	probably benign	0.03
R6674:Vmn1r225	UTSW	17	20,723,377 (GRCm39)	missense	probably benign	0.06
R7003:Vmn1r225	UTSW	17	20,723,416 (GRCm39)	missense	probably null	0.01
R7143:Vmn1r225	UTSW	17	20,722,646 (GRCm39)	missense	probably benign	0.22
R7422:Vmn1r225	UTSW	17	20,723,059 (GRCm39)	missense	probably benign	0.25
R7651:Vmn1r225	UTSW	17	20,722,611 (GRCm39)	missense	possibly damaging	0.56
R7952:Vmn1r225	UTSW	17	20,722,589 (GRCm39)	missense	probably damaging	0.98
R8097:Vmn1r225	UTSW	17	20,722,611 (GRCm39)	missense	possibly damaging	0.56
R8823:Vmn1r225	UTSW	17	20,722,823 (GRCm39)	missense	probably benign	0.08
R9007:Vmn1r225	UTSW	17	20,723,449 (GRCm39)	missense	probably damaging	0.96
R9041:Vmn1r225	UTSW	17	20,722,577 (GRCm39)	missense	possibly damaging	0.53
R9147:Vmn1r225	UTSW	17	20,722,577 (GRCm39)	missense	possibly damaging	0.53
R9148:Vmn1r225	UTSW	17	20,722,577 (GRCm39)	missense	possibly damaging	0.53
R9312:Vmn1r225	UTSW	17	20,722,960 (GRCm39)	missense	probably benign	0.39
R9401:Vmn1r225	UTSW	17	20,722,912 (GRCm39)	missense	probably damaging	1.00
R9401:Vmn1r225	UTSW	17	20,722,911 (GRCm39)	nonsense	probably null
R9488:Vmn1r225	UTSW	17	20,722,793 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn1r225	UTSW	17	20,722,753 (GRCm39)	missense	possibly damaging	0.65

Predicted Primers

PCR Primer
(F):5'- TGTCTCCTGAAAACAGAGCCAC -3'
(R):5'- TACAACTGGTGAAGATCCTGTG -3'

Sequencing Primer
(F):5'- CACAGAAAATCCTCACCCTGGTTTG -3'
(R):5'- CTGGTGAAGATCCTGTGACTATAGAC -3'

Posted On

2021-04-30