Mutagenetix > Incidental Mutation

Incidental Mutation 'R8703:Iqcm'

669154

Institutional Source

Beutler Lab

Gene Symbol

Iqcm

Ensembl Gene

ENSMUSG00000031620

Gene Name

IQ motif containing M

Synonyms

1700007B14Rik

MMRRC Submission

068557-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R8703 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

75448694-75984503 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 75888643 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Cysteine at position 451 (G451C)

Ref Sequence

ENSEMBL: ENSMUSP00000034033 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034033] [ENSMUST00000121983] [ENSMUST00000212704]

AlphaFold

Q149I8

Predicted Effect

probably damaging
Transcript: ENSMUST00000034033
AA Change: G451C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000034033
Gene: ENSMUSG00000031620
AA Change: G451C

Domain	Start	End	E-Value	Type
IQ	281	303	2.54e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000121983
AA Change: G451C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000112901
Gene: ENSMUSG00000031620
AA Change: G451C

Domain	Start	End	E-Value	Type
IQ	281	303	2.54e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000212704
AA Change: G451C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (44/44)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrv1	G	A	13: 81,528,673	T2013I	probably damaging	Het
Ak9	A	T	10: 41,325,124	N137Y		Het
Apol10b	T	A	15: 77,588,697	E52D	probably damaging	Het
Calr3	A	T	8: 72,438,447	Y128N	probably damaging	Het
Dnah14	T	A	1: 181,666,011	L1669*	probably null	Het
Dppa3	A	T	6: 122,628,778	D97V	probably damaging	Het
Dync1li1	C	A	9: 114,723,261	P490T	probably damaging	Het
Eya4	A	G	10: 23,163,442	S85P	probably benign	Het
Fpr-rs4	T	A	17: 18,022,070	I113N	probably damaging	Het
Fryl	T	C	5: 73,090,654	D1040G	probably damaging	Het
Fsip2	A	T	2: 82,991,527	D5868V	probably damaging	Het
Gpr75	A	C	11: 30,891,890	Q265P	probably damaging	Het
Lgr5	A	G	10: 115,452,705	L678P	probably benign	Het
Mcm9	G	A	10: 53,629,977	P67L	probably damaging	Het
Naalad2	A	C	9: 18,378,712	Y207D	probably damaging	Het
Nrap	C	T	19: 56,335,271	D1325N	probably damaging	Het
Nup133	A	G	8: 123,916,282		probably null	Het
Ogfr	GGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCCAAAGCCAGGTGG	GGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCCAAAGCCAGGTGG	2: 180,595,266		probably benign	Het
Olfr1066	C	A	2: 86,455,900	V124L	possibly damaging	Het
Olfr1272	T	A	2: 90,296,493	I123F	probably damaging	Het
Olfr1499	G	T	19: 13,814,741	P283H	probably damaging	Het
Per2	T	A	1: 91,424,045	N913I	possibly damaging	Het
Rab9	C	T	X: 166,457,758	D186N	probably benign	Het
Reps1	C	T	10: 18,093,242	R183C	probably damaging	Het
Rims1	A	T	1: 22,425,887	C134S		Het
Rpl10-ps3	A	G	9: 50,344,884	S20P	probably benign	Het
Sash1	C	A	10: 8,729,831	G932C	probably damaging	Het
Sema4d	T	C	13: 51,700,923	Q46R		Het
Sh3d19	A	G	3: 86,107,261	E453G	probably damaging	Het
Sirt6	A	G	10: 81,625,714		probably benign	Het
Sis	A	T	3: 72,960,324	F129L	probably damaging	Het
Skint5	T	A	4: 113,876,010	I399F	unknown	Het
Slc22a7	T	C	17: 46,434,025	D365G	probably damaging	Het
Slc6a12	A	G	6: 121,347,488	M38V	probably benign	Het
Slc9b2	C	A	3: 135,326,163	Y261*	probably null	Het
Spata21	A	G	4: 141,104,907	I460V	probably benign	Het
Tes	C	G	6: 17,099,789	P261R	probably damaging	Het
Tex15	T	C	8: 33,572,696	L718P	probably benign	Het
Tmem135	G	A	7: 89,158,962	A202V	probably benign	Het
Usp29	T	C	7: 6,961,322	F55L	probably benign	Het
Usp9y	A	G	Y: 1,356,317	L1128P	probably damaging	Het
Zdhhc5	T	C	2: 84,690,252	E527G	probably benign	Het
Zfand4	A	G	6: 116,273,643	I11M	probably damaging	Het
Zfp110	T	A	7: 12,848,961	I512K	probably benign	Het
Zfp638	T	A	6: 83,977,161	F1416Y	probably damaging	Het

Other mutations in Iqcm

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01895:Iqcm	APN	8	75888560	missense	probably damaging	1.00
IGL02835:Iqcm	APN	8	75554883	utr 5 prime	probably benign
R0056:Iqcm	UTSW	8	75753386	missense	probably benign
R2146:Iqcm	UTSW	8	75888613	missense	probably damaging	1.00
R2910:Iqcm	UTSW	8	75714776	missense	probably benign
R3801:Iqcm	UTSW	8	75669393	missense	possibly damaging	0.59
R3804:Iqcm	UTSW	8	75669393	missense	possibly damaging	0.59
R3834:Iqcm	UTSW	8	75577752	missense	possibly damaging	0.93
R3897:Iqcm	UTSW	8	75753400	missense	probably damaging	1.00
R4447:Iqcm	UTSW	8	75629766	missense	probably damaging	0.97
R4448:Iqcm	UTSW	8	75629766	missense	probably damaging	0.97
R4450:Iqcm	UTSW	8	75629766	missense	probably damaging	0.97
R4687:Iqcm	UTSW	8	75762989	missense	probably damaging	1.00
R4810:Iqcm	UTSW	8	75888653	missense	probably damaging	1.00
R4845:Iqcm	UTSW	8	75746352	missense	probably damaging	0.99
R4856:Iqcm	UTSW	8	75888600	missense	possibly damaging	0.95
R4886:Iqcm	UTSW	8	75888600	missense	possibly damaging	0.95
R5063:Iqcm	UTSW	8	75746286	missense	probably damaging	1.00
R5460:Iqcm	UTSW	8	75714789	missense	probably benign
R6403:Iqcm	UTSW	8	75577996	critical splice donor site	probably null
R6667:Iqcm	UTSW	8	75753352	missense	probably damaging	1.00
R7187:Iqcm	UTSW	8	75753416	missense	probably benign	0.22
R7263:Iqcm	UTSW	8	75763073	missense	probably benign
R7701:Iqcm	UTSW	8	75554911	missense	probably benign	0.02
R7916:Iqcm	UTSW	8	75577950	missense	probably benign
R7938:Iqcm	UTSW	8	75577968	missense	probably benign
R7974:Iqcm	UTSW	8	75554892	start codon destroyed	probably null	0.66
R8039:Iqcm	UTSW	8	75763105	missense	probably damaging	1.00
R8311:Iqcm	UTSW	8	75753490	splice site	probably benign
R9175:Iqcm	UTSW	8	75984239	missense	possibly damaging	0.84
R9475:Iqcm	UTSW	8	75753455	missense	probably damaging	1.00
RF002:Iqcm	UTSW	8	75577899	missense	probably benign	0.01
X0018:Iqcm	UTSW	8	75984212	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGATGCAGGTCAATCCATGTTTTG -3'
(R):5'- TTCTACAACACTAGTGAGAGCAC -3'

Sequencing Primer
(F):5'- CTCTCTTACAGATGGTCAAGG -3'
(R):5'- GTGAGAGCACAAAAGCACAAAAC -3'

Posted On

2021-04-30