Incidental Mutation 'R8716:Gstcd'
ID 669858
Institutional Source Beutler Lab
Gene Symbol Gstcd
Ensembl Gene ENSMUSG00000028018
Gene Name glutathione S-transferase, C-terminal domain containing
Synonyms 4933434L15Rik
MMRRC Submission 068569-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R8716 (G1)
Quality Score 225.009
Status Not validated
Chromosome 3
Chromosomal Location 132687513-132797794 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 132688950 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glycine at position 600 (D600G)
Ref Sequence ENSEMBL: ENSMUSP00000029651 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000029651] [ENSMUST00000080583]
AlphaFold Q5RL51
Predicted Effect probably damaging
Transcript: ENSMUST00000029651
AA Change: D600G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000029651
Gene: ENSMUSG00000028018
AA Change: D600G

DomainStartEndE-ValueType
low complexity region 52 62 N/A INTRINSIC
low complexity region 168 181 N/A INTRINSIC
low complexity region 195 205 N/A INTRINSIC
low complexity region 208 229 N/A INTRINSIC
Pfam:GidB 417 515 9e-9 PFAM
Pfam:Methyltransf_32 424 548 1.1e-15 PFAM
Pfam:MTS 430 508 5.3e-6 PFAM
Pfam:Methyltransf_31 440 571 2.6e-11 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000080583
AA Change: D600G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000079421
Gene: ENSMUSG00000028018
AA Change: D600G

DomainStartEndE-ValueType
low complexity region 52 62 N/A INTRINSIC
low complexity region 168 181 N/A INTRINSIC
low complexity region 195 205 N/A INTRINSIC
low complexity region 208 229 N/A INTRINSIC
Pfam:GidB 418 516 1.5e-8 PFAM
Pfam:Methyltransf_32 424 548 1.1e-15 PFAM
Pfam:Methyltransf_31 440 569 2.5e-11 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.9%
  • 20x: 99.5%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 76 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca13 A G 11: 9,243,774 (GRCm39) Y1879C probably benign Het
Abce1 A T 8: 80,427,784 (GRCm39) I168N possibly damaging Het
Acan G A 7: 78,762,438 (GRCm39) R2004H probably damaging Het
Acsm4 T A 7: 119,307,883 (GRCm39) L340Q probably damaging Het
Actmap C A 7: 26,896,631 (GRCm39) P65T probably damaging Het
Adamts2 T A 11: 50,664,091 (GRCm39) N342K probably damaging Het
Adgrd1 A T 5: 129,265,435 (GRCm39) D725V possibly damaging Het
Ahnak T A 19: 8,986,438 (GRCm39) L2574Q probably damaging Het
Ank2 A T 3: 126,736,488 (GRCm39) L3132* probably null Het
Ap3b2 T G 7: 81,126,901 (GRCm39) E283A probably benign Het
Asb17 A T 3: 153,559,151 (GRCm39) L287F probably damaging Het
Atr T G 9: 95,789,468 (GRCm39) N1541K probably benign Het
Bcas3 G A 11: 85,471,868 (GRCm39) V711I probably damaging Het
Ccdc82 T G 9: 13,252,922 (GRCm39) Y262* probably null Het
Cd209d CAT C 8: 3,923,772 (GRCm39) probably null Het
Cdc23 GAGACTACCGAAGA GAGA 18: 34,784,735 (GRCm39) probably null Het
Cfap54 T A 10: 92,800,494 (GRCm39) L1571F probably benign Het
Clca3b C T 3: 144,550,355 (GRCm39) V197I probably benign Het
Cul9 T C 17: 46,838,840 (GRCm39) S932G probably benign Het
Cyp3a59 T A 5: 146,033,411 (GRCm39) D182E probably damaging Het
Ddx19a T C 8: 111,710,243 (GRCm39) E119G probably damaging Het
Dnah1 A G 14: 30,989,941 (GRCm39) probably benign Het
Dop1b T A 16: 93,577,673 (GRCm39) L1761* probably null Het
Ehd2 A G 7: 15,698,106 (GRCm39) S44P probably benign Het
Exoc2 T A 13: 31,095,227 (GRCm39) H223L probably damaging Het
Fam217a G A 13: 35,108,248 (GRCm39) probably benign Het
Farp1 A G 14: 121,479,855 (GRCm39) N382S probably benign Het
Gbf1 T A 19: 46,272,460 (GRCm39) L1633Q probably damaging Het
Gpn1 C T 5: 31,656,642 (GRCm39) T115I probably benign Het
Ighv16-1 A C 12: 114,032,616 (GRCm39) M62R probably benign Het
Igsf3 T G 3: 101,334,739 (GRCm39) V272G probably damaging Het
Itgb2 T C 10: 77,393,787 (GRCm39) V409A probably damaging Het
Kcnh8 C T 17: 53,284,780 (GRCm39) P917S probably benign Het
Kif23 T C 9: 61,844,477 (GRCm39) T114A probably damaging Het
Krt35 A G 11: 99,987,011 (GRCm39) M1T probably null Het
Lgals4 C T 7: 28,540,921 (GRCm39) R282C probably damaging Het
Lonp2 T C 8: 87,442,933 (GRCm39) I798T probably benign Het
Lrp2 T A 2: 69,274,138 (GRCm39) T3971S probably benign Het
LTO1 G T 7: 144,468,930 (GRCm39) probably benign Het
Mcc C A 18: 44,582,403 (GRCm39) V758F possibly damaging Het
Naip2 C T 13: 100,280,914 (GRCm39) V1433I probably benign Het
Ndfip1 T C 18: 38,585,384 (GRCm39) F133L probably damaging Het
Nlrc4 T A 17: 74,752,985 (GRCm39) E466V probably damaging Het
Nwd1 A G 8: 73,388,908 (GRCm39) D112G probably damaging Het
Opn4 G T 14: 34,315,819 (GRCm39) R405S probably benign Het
Or10q12 T C 19: 13,746,185 (GRCm39) S160P probably damaging Het
Or2i1 C T 17: 37,508,299 (GRCm39) V112M possibly damaging Het
Or4c110 T C 2: 88,832,060 (GRCm39) T191A Het
Or7e168 C T 9: 19,720,165 (GRCm39) L184F probably damaging Het
Pde11a T A 2: 75,848,238 (GRCm39) D863V probably damaging Het
Plat G T 8: 23,262,248 (GRCm39) G91W probably damaging Het
Plekhf1 A T 7: 37,921,322 (GRCm39) L82Q probably damaging Het
Pou4f3 G T 18: 42,528,593 (GRCm39) D179Y possibly damaging Het
Ppp1r12a G A 10: 108,096,749 (GRCm39) R713H probably damaging Het
Psg17 C A 7: 18,555,310 (GRCm39) G11W probably benign Het
Ptprh A T 7: 4,567,273 (GRCm39) V533D probably damaging Het
Ptprn2 A G 12: 117,219,168 (GRCm39) D933G possibly damaging Het
Rab7 T C 6: 87,989,369 (GRCm39) S34G probably damaging Het
Rfx6 A C 10: 51,557,968 (GRCm39) H147P probably damaging Het
Rprd2 T C 3: 95,684,105 (GRCm39) Y310C probably damaging Het
Samd11 A G 4: 156,333,727 (GRCm39) F201S probably benign Het
Sox7 A G 14: 64,186,037 (GRCm39) T358A probably benign Het
Tenm4 C T 7: 96,555,148 (GRCm39) P2618S probably benign Het
Tie1 G A 4: 118,339,935 (GRCm39) T364M possibly damaging Het
Ttn C A 2: 76,597,266 (GRCm39) L19882F probably damaging Het
Tyw1 G A 5: 130,298,065 (GRCm39) R202Q probably damaging Het
Ubr4 T C 4: 139,196,164 (GRCm39) W1267R unknown Het
Usp42 G A 5: 143,703,696 (GRCm39) P456S probably damaging Het
Vmn1r235 T C 17: 21,482,554 (GRCm39) V293A possibly damaging Het
Vmn2r90 T A 17: 17,924,343 (GRCm39) H47Q probably damaging Het
Vps13d T A 4: 144,802,348 (GRCm39) T3503S Het
Vwa5b2 T A 16: 20,415,026 (GRCm39) N349K probably benign Het
Zeb1 A T 18: 5,767,958 (GRCm39) Y823F probably damaging Het
Zfp273 T A 13: 67,974,053 (GRCm39) C394S probably damaging Het
Zfp286 G T 11: 62,671,817 (GRCm39) A147D unknown Het
Zfp831 A G 2: 174,547,049 (GRCm39) T1411A possibly damaging Het
Other mutations in Gstcd
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01540:Gstcd APN 3 132,692,175 (GRCm39) missense probably damaging 0.96
IGL01800:Gstcd APN 3 132,790,335 (GRCm39) critical splice donor site probably null
IGL02434:Gstcd APN 3 132,701,963 (GRCm39) splice site probably benign
IGL02720:Gstcd APN 3 132,777,722 (GRCm39) missense probably benign 0.00
R0367:Gstcd UTSW 3 132,692,138 (GRCm39) splice site probably benign
R0378:Gstcd UTSW 3 132,692,169 (GRCm39) missense probably damaging 1.00
R0382:Gstcd UTSW 3 132,692,169 (GRCm39) missense probably damaging 1.00
R0465:Gstcd UTSW 3 132,688,905 (GRCm39) missense probably benign 0.04
R1295:Gstcd UTSW 3 132,711,389 (GRCm39) missense probably damaging 1.00
R1861:Gstcd UTSW 3 132,688,868 (GRCm39) missense probably damaging 1.00
R2056:Gstcd UTSW 3 132,787,814 (GRCm39) missense probably benign 0.00
R2331:Gstcd UTSW 3 132,704,641 (GRCm39) missense probably damaging 0.98
R2513:Gstcd UTSW 3 132,788,082 (GRCm39) missense possibly damaging 0.87
R2513:Gstcd UTSW 3 132,788,081 (GRCm39) missense possibly damaging 0.59
R3547:Gstcd UTSW 3 132,790,599 (GRCm39) missense possibly damaging 0.86
R4022:Gstcd UTSW 3 132,787,829 (GRCm39) missense probably damaging 1.00
R4713:Gstcd UTSW 3 132,688,860 (GRCm39) missense probably damaging 1.00
R4877:Gstcd UTSW 3 132,711,314 (GRCm39) splice site probably benign
R5152:Gstcd UTSW 3 132,790,717 (GRCm39) missense possibly damaging 0.95
R5165:Gstcd UTSW 3 132,790,440 (GRCm39) missense probably damaging 0.99
R6106:Gstcd UTSW 3 132,704,675 (GRCm39) missense probably benign 0.02
R6177:Gstcd UTSW 3 132,787,834 (GRCm39) missense probably damaging 1.00
R7100:Gstcd UTSW 3 132,790,704 (GRCm39) missense probably benign 0.01
R7264:Gstcd UTSW 3 132,790,540 (GRCm39) missense probably benign 0.00
R7785:Gstcd UTSW 3 132,787,868 (GRCm39) missense probably damaging 0.99
R7786:Gstcd UTSW 3 132,787,868 (GRCm39) missense probably damaging 0.99
R7972:Gstcd UTSW 3 132,777,894 (GRCm39) missense probably benign 0.10
R7975:Gstcd UTSW 3 132,777,863 (GRCm39) missense probably damaging 1.00
R8029:Gstcd UTSW 3 132,787,868 (GRCm39) missense probably damaging 0.99
R8036:Gstcd UTSW 3 132,787,868 (GRCm39) missense probably damaging 0.99
R8088:Gstcd UTSW 3 132,787,868 (GRCm39) missense probably damaging 0.99
R8722:Gstcd UTSW 3 132,777,822 (GRCm39) nonsense probably null
R9276:Gstcd UTSW 3 132,777,665 (GRCm39) missense probably damaging 1.00
R9575:Gstcd UTSW 3 132,704,708 (GRCm39) missense probably damaging 1.00
R9789:Gstcd UTSW 3 132,790,357 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TTCCAGGAGCACAGCAAAG -3'
(R):5'- AGCCTTCTGGAAATTGCTAGCC -3'

Sequencing Primer
(F):5'- AAAGGCTCCCTGGACGG -3'
(R):5'- GGTGATCCACTCTAAGCTTACAG -3'
Posted On 2021-04-30