Mutagenetix > Incidental Mutation

Incidental Mutation 'R0677:Vmn2r114'

67068

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r114

Ensembl Gene

ENSMUSG00000091945

Gene Name

vomeronasal 2, receptor 114

Synonyms

EG666002

MMRRC Submission

038862-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.131) question?

Stock #

R0677 (G1)

Quality Score

139

Status

Not validated

Chromosome

Chromosomal Location

23509908-23531287 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 23529568 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Alanine at position 178 (D178A)

Ref Sequence

ENSEMBL: ENSMUSP00000127505 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168033]

AlphaFold

E9Q281

Predicted Effect

probably damaging
Transcript: ENSMUST00000168033
AA Change: D178A

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000127505
Gene: ENSMUSG00000091945
AA Change: D178A

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	154	470	1.5e-24	PFAM
Pfam:NCD3G	511	564	1.5e-18	PFAM
Pfam:7tm_3	597	832	1.4e-55	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.6%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 25 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A630073D07Rik	G	A	6: 132,603,520 (GRCm39)	Q79*	probably null	Het
Apol8	A	C	15: 77,634,051 (GRCm39)	I175S	probably damaging	Het
BC004004	T	C	17: 29,517,638 (GRCm39)	F284S	probably damaging	Het
Cul7	T	A	17: 46,974,116 (GRCm39)	L1467H	probably damaging	Het
Dchs1	G	A	7: 105,414,191 (GRCm39)	R875C	probably damaging	Het
Depdc5	A	C	5: 33,058,814 (GRCm39)	N261T	probably damaging	Het
Flrt1	A	T	19: 7,073,544 (GRCm39)	C334*	probably null	Het
Galnt5	T	A	2: 57,888,992 (GRCm39)	Y197*	probably null	Het
Gp5	A	G	16: 30,127,193 (GRCm39)	S494P	probably benign	Het
Ifnlr1	T	A	4: 135,432,945 (GRCm39)	D460E	possibly damaging	Het
Mrgpra4	C	T	7: 47,630,728 (GRCm39)	S291N	probably benign	Het
Msh4	G	A	3: 153,585,004 (GRCm39)	P367S	possibly damaging	Het
Myct1	G	T	10: 5,554,261 (GRCm39)	V43F	probably benign	Het
Ndst2	G	A	14: 20,779,647 (GRCm39)	R198W	probably benign	Het
Ogdhl	A	T	14: 32,061,882 (GRCm39)	H500L	probably damaging	Het
Or52s1	C	T	7: 102,862,005 (GRCm39)	R302*	probably null	Het
Or6c2b	T	A	10: 128,947,947 (GRCm39)	M116L	probably damaging	Het
Pkhd1	C	T	1: 20,594,454 (GRCm39)	G1220S	probably benign	Het
Slc26a4	A	G	12: 31,599,910 (GRCm39)		probably null	Het
Spata31g1	T	A	4: 42,970,952 (GRCm39)	L59*	probably null	Het
Sytl1	A	T	4: 132,980,536 (GRCm39)	C551S	possibly damaging	Het
Uri1	T	C	7: 37,664,925 (GRCm39)	N256D	probably benign	Het
Vmn2r23	C	T	6: 123,690,410 (GRCm39)	L429F	probably benign	Het
Washc2	T	C	6: 116,221,577 (GRCm39)	L685P	probably damaging	Het
Wipi2	A	T	5: 142,643,989 (GRCm39)	I124F	probably damaging	Het

Other mutations in Vmn2r114

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Vmn2r114	APN	17	23,510,639 (GRCm39)	missense	probably damaging	1.00
IGL00990:Vmn2r114	APN	17	23,510,212 (GRCm39)	missense	probably damaging	1.00
IGL00990:Vmn2r114	APN	17	23,509,957 (GRCm39)	missense	probably benign	0.23
IGL00990:Vmn2r114	APN	17	23,509,939 (GRCm39)	missense	probably benign
IGL01838:Vmn2r114	APN	17	23,515,956 (GRCm39)	missense	probably benign	0.44
IGL01990:Vmn2r114	APN	17	23,529,355 (GRCm39)	missense	probably benign	0.22
IGL01994:Vmn2r114	APN	17	23,529,451 (GRCm39)	missense	probably damaging	1.00
IGL02153:Vmn2r114	APN	17	23,510,782 (GRCm39)	missense	probably benign	0.01
IGL02453:Vmn2r114	APN	17	23,530,108 (GRCm39)	missense	probably benign	0.00
IGL02621:Vmn2r114	APN	17	23,529,494 (GRCm39)	missense	probably damaging	0.98
IGL02938:Vmn2r114	APN	17	23,510,263 (GRCm39)	missense	probably benign	0.10
IGL03130:Vmn2r114	APN	17	23,515,970 (GRCm39)	splice site	probably benign
IGL03325:Vmn2r114	APN	17	23,510,652 (GRCm39)	missense	probably damaging	1.00
BB004:Vmn2r114	UTSW	17	23,510,619 (GRCm39)	missense	probably damaging	1.00
R0109:Vmn2r114	UTSW	17	23,529,549 (GRCm39)	nonsense	probably null
R0164:Vmn2r114	UTSW	17	23,528,800 (GRCm39)	critical splice donor site	probably null
R0310:Vmn2r114	UTSW	17	23,509,917 (GRCm39)	missense	probably benign	0.23
R0583:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1127:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1147:Vmn2r114	UTSW	17	23,530,037 (GRCm39)	missense	probably benign	0.00
R1147:Vmn2r114	UTSW	17	23,530,037 (GRCm39)	missense	probably benign	0.00
R1157:Vmn2r114	UTSW	17	23,529,314 (GRCm39)	missense	possibly damaging	0.60
R1323:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1347:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1435:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1437:Vmn2r114	UTSW	17	23,510,185 (GRCm39)	missense	probably damaging	1.00
R1585:Vmn2r114	UTSW	17	23,510,675 (GRCm39)	missense	probably damaging	0.98
R1641:Vmn2r114	UTSW	17	23,515,962 (GRCm39)	missense	probably benign	0.00
R1748:Vmn2r114	UTSW	17	23,527,035 (GRCm39)	missense	probably benign	0.17
R1954:Vmn2r114	UTSW	17	23,530,086 (GRCm39)	missense	probably benign	0.32
R2081:Vmn2r114	UTSW	17	23,510,083 (GRCm39)	missense	possibly damaging	0.91
R2103:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2113:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2134:Vmn2r114	UTSW	17	23,510,737 (GRCm39)	missense	probably damaging	1.00
R2149:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2424:Vmn2r114	UTSW	17	23,515,842 (GRCm39)	missense	possibly damaging	0.90
R2847:Vmn2r114	UTSW	17	23,509,948 (GRCm39)	missense	probably benign	0.00
R2848:Vmn2r114	UTSW	17	23,509,948 (GRCm39)	missense	probably benign	0.00
R2893:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3017:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3018:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3019:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3020:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3021:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R4628:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R4668:Vmn2r114	UTSW	17	23,529,447 (GRCm39)	missense	possibly damaging	0.83
R4840:Vmn2r114	UTSW	17	23,510,353 (GRCm39)	missense	probably damaging	0.97
R4841:Vmn2r114	UTSW	17	23,529,336 (GRCm39)	missense	probably benign	0.04
R4842:Vmn2r114	UTSW	17	23,529,336 (GRCm39)	missense	probably benign	0.04
R4856:Vmn2r114	UTSW	17	23,527,008 (GRCm39)	missense	probably benign	0.11
R4886:Vmn2r114	UTSW	17	23,527,008 (GRCm39)	missense	probably benign	0.11
R4992:Vmn2r114	UTSW	17	23,510,765 (GRCm39)	missense	probably benign	0.03
R5182:Vmn2r114	UTSW	17	23,510,632 (GRCm39)	missense	probably damaging	0.96
R5223:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5405:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5449:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5615:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5834:Vmn2r114	UTSW	17	23,529,599 (GRCm39)	missense	possibly damaging	0.90
R6150:Vmn2r114	UTSW	17	23,510,269 (GRCm39)	missense	probably benign	0.03
R6277:Vmn2r114	UTSW	17	23,509,954 (GRCm39)	missense	possibly damaging	0.93
R6403:Vmn2r114	UTSW	17	23,528,939 (GRCm39)	missense	probably damaging	0.99
R6589:Vmn2r114	UTSW	17	23,510,642 (GRCm39)	missense	probably damaging	1.00
R6613:Vmn2r114	UTSW	17	23,529,220 (GRCm39)	missense	possibly damaging	0.82
R6747:Vmn2r114	UTSW	17	23,528,850 (GRCm39)	missense	probably benign	0.00
R6837:Vmn2r114	UTSW	17	23,529,176 (GRCm39)	missense	probably benign	0.10
R6911:Vmn2r114	UTSW	17	23,510,104 (GRCm39)	missense	probably damaging	0.98
R6950:Vmn2r114	UTSW	17	23,529,137 (GRCm39)	missense	probably benign	0.03
R7276:Vmn2r114	UTSW	17	23,509,934 (GRCm39)	missense	probably damaging	0.97
R7482:Vmn2r114	UTSW	17	23,510,468 (GRCm39)	missense	probably damaging	1.00
R7514:Vmn2r114	UTSW	17	23,527,035 (GRCm39)	missense	probably null	0.96
R7523:Vmn2r114	UTSW	17	23,529,611 (GRCm39)	missense	probably benign	0.01
R7563:Vmn2r114	UTSW	17	23,510,000 (GRCm39)	missense	probably benign	0.01
R7585:Vmn2r114	UTSW	17	23,510,239 (GRCm39)	missense	probably damaging	1.00
R7593:Vmn2r114	UTSW	17	23,510,817 (GRCm39)	nonsense	probably null
R7611:Vmn2r114	UTSW	17	23,515,944 (GRCm39)	missense	probably damaging	0.97
R7641:Vmn2r114	UTSW	17	23,527,177 (GRCm39)	missense	possibly damaging	0.53
R7651:Vmn2r114	UTSW	17	23,509,986 (GRCm39)	nonsense	probably null
R7970:Vmn2r114	UTSW	17	23,530,186 (GRCm39)	missense	probably benign	0.00
R8737:Vmn2r114	UTSW	17	23,529,142 (GRCm39)	missense	probably benign	0.36
R8802:Vmn2r114	UTSW	17	23,528,836 (GRCm39)	missense	possibly damaging	0.65
R8847:Vmn2r114	UTSW	17	23,528,986 (GRCm39)	missense	probably damaging	1.00
R8991:Vmn2r114	UTSW	17	23,529,286 (GRCm39)	missense	probably damaging	1.00
R9138:Vmn2r114	UTSW	17	23,510,578 (GRCm39)	missense	probably damaging	1.00
R9173:Vmn2r114	UTSW	17	23,510,527 (GRCm39)	missense	probably damaging	0.99
R9175:Vmn2r114	UTSW	17	23,527,212 (GRCm39)	missense	probably damaging	1.00
R9657:Vmn2r114	UTSW	17	23,510,690 (GRCm39)	missense	probably damaging	1.00
R9670:Vmn2r114	UTSW	17	23,531,098 (GRCm39)	missense
X0065:Vmn2r114	UTSW	17	23,529,931 (GRCm39)	missense	probably benign	0.34
Z1088:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null

Predicted Primers

PCR Primer
(F):5'- CCACTGTGAGGTGGTGATCCATAGTC -3'
(R):5'- CCTGGGCAAATCTAAGTAGTTCAAGGG -3'

Sequencing Primer
(F):5'- GCTCACAGCTAAGGTACTGTCTG -3'
(R):5'- GTAGTTCAAGGGTACTATCACAGAC -3'

Posted On

2013-08-21