Mutagenetix > Incidental Mutation

Incidental Mutation 'R8795:Vmn2r104'

671226

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r104

Ensembl Gene

ENSMUSG00000090315

Gene Name

vomeronasal 2, receptor 104

Synonyms

V2r7

MMRRC Submission

068636-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.124) question?

Stock #

R8795 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

20029425-20048205 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 20042726 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 158 (I158L)

Ref Sequence

ENSEMBL: ENSMUSP00000129895 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168050]

AlphaFold

E9Q2J5

Predicted Effect

probably benign
Transcript: ENSMUST00000168050
AA Change: I158L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000129895
Gene: ENSMUSG00000090315
AA Change: I158L

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	85	457	4e-38	PFAM
Pfam:NCD3G	512	565	2.1e-20	PFAM
Pfam:7tm_3	598	833	1.7e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

97% (72/74)

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aars2	A	T	17: 45,507,672	M128L	probably damaging	Het
Abcc12	A	G	8: 86,531,584	S768P	possibly damaging	Het
Adamts8	A	T	9: 30,943,188	M118L	probably benign	Het
Adgrl4	A	G	3: 151,510,779	N533S	probably benign	Het
Agk	T	G	6: 40,386,920	I278R	possibly damaging	Het
Atr	T	A	9: 95,867,531	W466R	probably damaging	Het
Cacna1h	C	T	17: 25,393,564	V387I	probably damaging	Het
Cand2	T	G	6: 115,786,928	D270E	probably benign	Het
Ccdc107	C	T	4: 43,495,514	T139M	probably damaging	Het
Cenpl	A	G	1: 161,083,014	E177G	probably benign	Het
Clint1	C	A	11: 45,884,351	Q56K	probably damaging	Het
Col9a1	A	G	1: 24,194,731	R249G	unknown	Het
Cpne5	C	T	17: 29,204,688		probably benign	Het
Ddx27	A	G	2: 167,017,810	D54G	probably benign	Het
Dync2h1	C	T	9: 7,137,087	E1468K	probably benign	Het
E130308A19Rik	A	T	4: 59,737,676	N429I	possibly damaging	Het
Endov	G	T	11: 119,499,554	G86C	possibly damaging	Het
Ercc5	A	T	1: 44,163,929	Y242F	possibly damaging	Het
Gabrr1	T	A	4: 33,161,756	V360E	probably damaging	Het
Gcn1l1	T	C	5: 115,614,395	I2153T	probably benign	Het
Gje1	T	C	10: 14,718,126	N8S	probably benign	Het
Gm12394	A	G	4: 42,792,992	V380A	probably benign	Het
Greb1l	A	G	18: 10,553,739	H1580R	probably damaging	Het
Grin1	C	G	2: 25,297,456	S614T	probably damaging	Het
Hmcn2	A	G	2: 31,425,381	N3714S	probably benign	Het
Idh1	CA	CAA	1: 65,165,188		probably null	Het
Ifitm2	A	T	7: 140,955,748	H56Q	probably damaging	Het
Ifrd1	G	A	12: 40,213,077	T218I	possibly damaging	Het
Igkv1-99	G	T	6: 68,542,386	G109V		Het
Impg2	A	G	16: 56,260,248	E805G	probably benign	Het
Itgb6	T	A	2: 60,653,285	N260Y	probably damaging	Het
Maneal	G	T	4: 124,856,690	Y424*	probably null	Het
Map4k2	T	G	19: 6,351,610	C677W	probably damaging	Het
Med6	T	C	12: 81,591,260	H59R	probably benign	Het
Mms22l	T	A	4: 24,536,245	D571E	probably benign	Het
Mroh8	A	G	2: 157,225,573	F622S	probably damaging	Het
Mrpl44	A	T	1: 79,776,257	Q42L	probably damaging	Het
Myh8	G	T	11: 67,283,377		probably benign	Het
Ndufb2	T	C	6: 39,592,652	V13A	probably benign	Het
Nf1	T	A	11: 79,425,616	L499Q	probably damaging	Het
Olfr1185-ps1	T	G	2: 88,499,511	V142G	probably damaging	Het
Olfr1281	T	A	2: 111,328,536	L39*	probably null	Het
Olfr1357	T	C	10: 78,611,864	Y259C	probably damaging	Het
Olfr782	T	C	10: 129,351,325	I254T	probably damaging	Het
Opa1	T	C	16: 29,629,632	C874R	probably damaging	Het
Pld3	T	A	7: 27,535,861	D314V	possibly damaging	Het
Pou6f1	A	C	15: 100,587,805	C114W	possibly damaging	Het
Pqlc3	A	G	12: 16,993,480	W150R	probably damaging	Het
Prl2c1	A	C	13: 27,849,406	Q2P	possibly damaging	Het
Prl2c1	G	T	13: 27,849,407	Q2H	probably benign	Het
Pzp	C	A	6: 128,494,738	K908N	probably damaging	Het
Rab6b	G	A	9: 103,162,626	G125D	probably damaging	Het
Rhbdd2	C	T	5: 135,635,131	T69I	probably benign	Het
Samd9l	A	T	6: 3,374,221	Y1013*	probably null	Het
Slc13a4	T	A	6: 35,283,295	T217S	probably benign	Het
Smarcad1	T	C	6: 65,072,049	L253S	probably benign	Het
Snrpa	C	A	7: 27,191,609	V146L	possibly damaging	Het
Srrt	A	T	5: 137,299,976	D311E	probably benign	Het
Stk32b	A	T	5: 37,649,139	F20L	probably damaging	Het
Sult2a7	T	C	7: 14,490,089	T136A	probably benign	Het
Sv2a	G	A	3: 96,187,080	V244I	probably benign	Het
Tesk1	T	A	4: 43,446,070		probably null	Het
Tmem260	A	G	14: 48,451,913	H63R	probably damaging	Het
Ugt1a6b	A	G	1: 88,107,072	E44G	probably benign	Het
Unc79	A	T	12: 103,108,254	I1363F	probably damaging	Het
Usp15	T	A	10: 123,153,048	N255Y	probably benign	Het
Usp35	T	C	7: 97,312,063	N719D	probably benign	Het
Usp35	A	G	7: 97,311,960	V753A	possibly damaging	Het
Vmn2r69	A	T	7: 85,415,675	M1K	probably null	Het
Zan	C	T	5: 137,398,260	E4345K	unknown	Het
Zc3h7a	A	T	16: 11,147,283	M662K	possibly damaging	Het
Zfp512	G	A	5: 31,476,790	V438M	probably damaging	Het
Zfy1	T	C	Y: 738,945	D87G	unknown	Het
Zmym4	A	T	4: 126,906,026	V653E	probably benign	Het

Other mutations in Vmn2r104

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00427:Vmn2r104	APN	17	20038239	missense	probably damaging	0.98
IGL01098:Vmn2r104	APN	17	20048096	missense	probably benign	0.27
IGL01333:Vmn2r104	APN	17	20042793	missense	probably benign	0.17
IGL01527:Vmn2r104	APN	17	20042896	missense	possibly damaging	0.82
IGL01773:Vmn2r104	APN	17	20040668	missense	probably benign	0.10
IGL01939:Vmn2r104	APN	17	20029925	missense	probably damaging	0.99
IGL02121:Vmn2r104	APN	17	20041794	nonsense	probably null
IGL02305:Vmn2r104	APN	17	20042856	missense	probably benign	0.09
IGL02374:Vmn2r104	APN	17	20042786	missense	probably benign	0.34
IGL03260:Vmn2r104	APN	17	20042821	missense	probably benign	0.05
IGL03366:Vmn2r104	APN	17	20029604	missense	probably damaging	1.00
R0091:Vmn2r104	UTSW	17	20041813	missense	possibly damaging	0.79
R0125:Vmn2r104	UTSW	17	20029807	missense	probably damaging	0.98
R0257:Vmn2r104	UTSW	17	20029627	missense	probably damaging	1.00
R0381:Vmn2r104	UTSW	17	20048002	nonsense	probably null
R0709:Vmn2r104	UTSW	17	20042904	missense	probably damaging	1.00
R0786:Vmn2r104	UTSW	17	20042725	missense	probably benign
R1575:Vmn2r104	UTSW	17	20042215	missense	probably damaging	1.00
R1827:Vmn2r104	UTSW	17	20042235	missense	probably damaging	0.97
R1932:Vmn2r104	UTSW	17	20040769	missense	probably damaging	1.00
R1956:Vmn2r104	UTSW	17	20042051	missense	probably damaging	0.98
R2203:Vmn2r104	UTSW	17	20029821	missense	probably benign	0.05
R2205:Vmn2r104	UTSW	17	20029821	missense	probably benign	0.05
R2859:Vmn2r104	UTSW	17	20048193	missense	possibly damaging	0.82
R3701:Vmn2r104	UTSW	17	20029556	missense	probably damaging	1.00
R3834:Vmn2r104	UTSW	17	20029921	missense	probably benign	0.02
R4151:Vmn2r104	UTSW	17	20029885	missense	probably damaging	1.00
R4470:Vmn2r104	UTSW	17	20042241	missense	probably damaging	1.00
R4625:Vmn2r104	UTSW	17	20048181	missense	probably benign	0.00
R4754:Vmn2r104	UTSW	17	20040768	nonsense	probably null
R4911:Vmn2r104	UTSW	17	20030026	missense	probably benign	0.00
R5270:Vmn2r104	UTSW	17	20038266	missense	probably damaging	1.00
R5279:Vmn2r104	UTSW	17	20041884	missense	probably benign	0.07
R5311:Vmn2r104	UTSW	17	20029901	missense	probably damaging	1.00
R5370:Vmn2r104	UTSW	17	20030188	missense	probably damaging	0.97
R5461:Vmn2r104	UTSW	17	20030081	missense	probably damaging	1.00
R5683:Vmn2r104	UTSW	17	20040719	nonsense	probably null
R5795:Vmn2r104	UTSW	17	20030110	missense	probably benign	0.02
R5795:Vmn2r104	UTSW	17	20030282	missense	possibly damaging	0.89
R5970:Vmn2r104	UTSW	17	20029471	missense	probably benign	0.01
R5983:Vmn2r104	UTSW	17	20041708	missense	probably damaging	1.00
R5992:Vmn2r104	UTSW	17	20029485	missense	probably damaging	1.00
R6066:Vmn2r104	UTSW	17	20038311	missense	possibly damaging	0.69
R6156:Vmn2r104	UTSW	17	20041647	missense	probably damaging	1.00
R6182:Vmn2r104	UTSW	17	20030245	missense	probably benign	0.16
R6245:Vmn2r104	UTSW	17	20041567	missense	possibly damaging	0.69
R6333:Vmn2r104	UTSW	17	20029586	missense	probably benign	0.30
R6573:Vmn2r104	UTSW	17	20042225	missense	probably damaging	1.00
R7101:Vmn2r104	UTSW	17	20030096	missense	possibly damaging	0.65
R7123:Vmn2r104	UTSW	17	20040826	missense	probably benign	0.12
R7485:Vmn2r104	UTSW	17	20029475	missense	probably benign	0.01
R7514:Vmn2r104	UTSW	17	20029529	missense	probably damaging	1.00
R7634:Vmn2r104	UTSW	17	20041709	missense	possibly damaging	0.48
R7958:Vmn2r104	UTSW	17	20042726	missense	probably benign
R8031:Vmn2r104	UTSW	17	20042786	missense	probably benign	0.34
R8094:Vmn2r104	UTSW	17	20030221	missense	possibly damaging	0.77
R8191:Vmn2r104	UTSW	17	20030203	missense	possibly damaging	0.89
R8308:Vmn2r104	UTSW	17	20040778	missense	possibly damaging	0.55
R8691:Vmn2r104	UTSW	17	20041848	missense	probably damaging	0.98
R8900:Vmn2r104	UTSW	17	20041662	missense	probably damaging	0.99
R8913:Vmn2r104	UTSW	17	20029706	missense	probably damaging	1.00
R9180:Vmn2r104	UTSW	17	20042825	missense	probably benign	0.00
R9199:Vmn2r104	UTSW	17	20041835	missense	probably damaging	0.99
R9282:Vmn2r104	UTSW	17	20040836	missense	probably damaging	1.00
R9303:Vmn2r104	UTSW	17	20048177	missense	possibly damaging	0.90
R9305:Vmn2r104	UTSW	17	20048177	missense	possibly damaging	0.90
R9322:Vmn2r104	UTSW	17	20042825	missense	probably benign	0.00
R9325:Vmn2r104	UTSW	17	20048171	missense	possibly damaging	0.95
R9414:Vmn2r104	UTSW	17	20029988	missense	probably damaging	0.99
R9785:Vmn2r104	UTSW	17	20048147	missense	probably benign
RF007:Vmn2r104	UTSW	17	20048040	missense	probably benign	0.36
Z1177:Vmn2r104	UTSW	17	20029789	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCAAGAGATTTCTGAATTGATCTCC -3'
(R):5'- CTGCTATTTGCCATTGAGGAG -3'

Sequencing Primer
(F):5'- CTGAATTGATCTCCATGTGTCTG -3'
(R):5'- GCTATTTGCCATTGAGGAGATCAAC -3'

Posted On

2021-04-30