Mutagenetix > Incidental Mutation

Incidental Mutation 'R8798:Tmem132c'

671363

Institutional Source

Beutler Lab

Gene Symbol

Tmem132c

Ensembl Gene

ENSMUSG00000034324

Gene Name

transmembrane protein 132C

Synonyms

2810482M11Rik, 4632425D07Rik

MMRRC Submission

068724-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.088) question?

Stock #

R8798 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

127318890-127642854 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 127437217 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 235 (Y235*)

Ref Sequence

ENSEMBL: ENSMUSP00000113090 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000119026]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000119026
AA Change: Y235*

SMART Domains

Protein: ENSMUSP00000113090
Gene: ENSMUSG00000034324
AA Change: Y235*

Domain	Start	End	E-Value	Type
low complexity region	14	21	N/A	INTRINSIC
Pfam:TMEM132D_N	58	187	1.2e-54	PFAM
Pfam:TMEM132	444	787	4.5e-143	PFAM
Pfam:TMEM132D_C	892	980	2.4e-39	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

100% (74/74)

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700011L22Rik	G	A	8: 79,937,380 (GRCm39)	R176*	probably null	Het
4921539E11Rik	A	C	4: 103,123,574 (GRCm39)		probably benign	Het
Abcc2	A	G	19: 43,797,105 (GRCm39)	N492S	probably benign	Het
Acox1	T	C	11: 116,065,183 (GRCm39)	Y624C	probably damaging	Het
Aff4	T	C	11: 53,291,335 (GRCm39)		probably benign	Het
Ak9	T	A	10: 41,258,847 (GRCm39)	D781E		Het
Arfgef3	T	C	10: 18,522,799 (GRCm39)	D409G	probably damaging	Het
Arhgef18	T	A	8: 3,414,645 (GRCm39)	Y8N	probably benign	Het
Blnk	A	G	19: 40,950,795 (GRCm39)	Y119H	probably damaging	Het
Brsk2	T	C	7: 141,541,601 (GRCm39)	L310P	probably damaging	Het
Cgas	G	A	9: 78,350,348 (GRCm39)	R5C	probably benign	Het
Cit	T	A	5: 116,107,102 (GRCm39)	L1078Q	probably damaging	Het
Cpn1	A	T	19: 43,974,675 (GRCm39)	V18D	possibly damaging	Het
Csmd3	A	T	15: 47,595,382 (GRCm39)	Y1115N		Het
Dnah9	A	C	11: 65,796,057 (GRCm39)	V3589G	probably damaging	Het
Dpp9	T	C	17: 56,506,037 (GRCm39)	Y454C	probably damaging	Het
Eif3i	A	G	4: 129,490,717 (GRCm39)	V67A	probably benign	Het
Eif6	T	C	2: 155,664,886 (GRCm39)	N200S	probably damaging	Het
Fbxo10	T	C	4: 45,051,605 (GRCm39)	H502R	possibly damaging	Het
Fcer1a	A	G	1: 173,053,047 (GRCm39)	Y50H	probably benign	Het
Filip1	G	A	9: 79,727,372 (GRCm39)	H416Y	possibly damaging	Het
Frmpd1	A	T	4: 45,285,424 (GRCm39)	N1415I	possibly damaging	Het
Gdpgp1	C	T	7: 79,888,725 (GRCm39)	P252L	probably damaging	Het
Gsap	T	C	5: 21,476,248 (GRCm39)		probably null	Het
Hgs	T	C	11: 120,370,938 (GRCm39)	V598A	probably benign	Het
Irf2	G	T	8: 47,260,349 (GRCm39)	V94L	probably benign	Het
Kalrn	G	T	16: 33,803,225 (GRCm39)	H2675Q	possibly damaging	Het
Klhl33	C	A	14: 51,130,565 (GRCm39)	A50S	possibly damaging	Het
Klk1b11	T	C	7: 43,645,372 (GRCm39)	I15T	probably benign	Het
Lcor	C	T	19: 41,573,698 (GRCm39)	R818W	probably damaging	Het
Lrrn3	A	G	12: 41,503,174 (GRCm39)	M381T	possibly damaging	Het
Ltbr	A	G	6: 125,284,258 (GRCm39)	Y395H	probably benign	Het
Ltf	A	G	9: 110,852,828 (GRCm39)		probably benign	Het
Ly75	A	G	2: 60,154,270 (GRCm39)	F1059S	probably benign	Het
Lypd6	T	A	2: 50,078,774 (GRCm39)	I90N	possibly damaging	Het
Map1a	G	A	2: 121,132,768 (GRCm39)	V1195M	probably benign	Het
Mroh2b	A	T	15: 4,978,191 (GRCm39)	I1320F	probably damaging	Het
Mug2	A	G	6: 122,058,569 (GRCm39)	T1324A	probably damaging	Het
Mylk	G	A	16: 34,719,772 (GRCm39)	V562M	possibly damaging	Het
Myo15b	G	A	11: 115,754,232 (GRCm39)	G911R		Het
Neo1	A	G	9: 58,820,449 (GRCm39)	Y825H	probably damaging	Het
Nfat5	T	G	8: 108,074,321 (GRCm39)	V343G	probably damaging	Het
Nlrp2	A	T	7: 5,330,887 (GRCm39)	I503K	possibly damaging	Het
Or10d5	G	A	9: 39,862,013 (GRCm39)	T18I	probably benign	Het
Or13d1	C	A	4: 52,970,790 (GRCm39)	H56Q	possibly damaging	Het
Or5k16	A	T	16: 58,736,307 (GRCm39)	D232E	probably benign	Het
Or8d2	G	A	9: 38,760,213 (GRCm39)	E268K	probably benign	Het
Pdcl2	T	C	5: 76,472,947 (GRCm39)	D7G	probably damaging	Het
Phyh	C	T	2: 4,923,893 (GRCm39)	R5C	probably damaging	Het
Prr11	A	C	11: 86,996,881 (GRCm39)	S28A	unknown	Het
Psmd13	T	A	7: 140,477,663 (GRCm39)	L190*	probably null	Het
Ptx4	A	G	17: 25,343,716 (GRCm39)	D322G	probably damaging	Het
Rnf40	T	C	7: 127,188,954 (GRCm39)	L109P	probably damaging	Het
Rrp1	T	C	10: 78,245,024 (GRCm39)	T102A	probably damaging	Het
Sema4a	T	G	3: 88,344,004 (GRCm39)	D749A	possibly damaging	Het
Serpina3f	A	T	12: 104,183,702 (GRCm39)	D188V	probably benign	Het
Smarca5	C	A	8: 81,443,137 (GRCm39)	A570S	probably damaging	Het
Spocd1	G	C	4: 129,823,997 (GRCm39)		probably null	Het
Stard9	G	A	2: 120,535,212 (GRCm39)	G3823D	probably benign	Het
Suco	A	G	1: 161,648,004 (GRCm39)	L1094S	probably damaging	Het
Tcf7	T	C	11: 52,151,421 (GRCm39)	D79G	probably damaging	Het
Tdrd12	A	G	7: 35,228,605 (GRCm39)	M39T	probably damaging	Het
Thnsl1	A	G	2: 21,217,209 (GRCm39)	N321S	probably benign	Het
Tnfaip6	T	C	2: 51,933,824 (GRCm39)	F60L	probably benign	Het
Tnpo3	T	C	6: 29,572,620 (GRCm39)	I411V	probably benign	Het
Trio	A	C	15: 27,851,923 (GRCm39)	V856G	possibly damaging	Het
Trpc2	C	G	7: 101,733,767 (GRCm39)	R239G	probably benign	Het
Ttn	C	T	2: 76,656,476 (GRCm39)	V12502I	unknown	Het
Usf3	A	G	16: 44,040,536 (GRCm39)	D1672G	probably damaging	Het
Usp24	A	G	4: 106,236,436 (GRCm39)	N1011S	probably benign	Het
Vac14	A	G	8: 111,446,519 (GRCm39)	E756G	probably benign	Het
Vmn2r41	A	G	7: 8,164,522 (GRCm39)	L10P	probably damaging	Het
Xrcc5	T	C	1: 72,353,337 (GRCm39)	M115T	probably damaging	Het
Zfp1006	G	A	8: 129,945,779 (GRCm39)	H349Y	probably damaging	Het
Zfp827	G	A	8: 79,916,463 (GRCm39)		probably benign	Het
Zmym1	A	T	4: 126,943,664 (GRCm39)	N241K	possibly damaging	Het

Other mutations in Tmem132c

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00869:Tmem132c	APN	5	127,640,306 (GRCm39)	missense	possibly damaging	0.95
IGL00985:Tmem132c	APN	5	127,581,930 (GRCm39)	missense	probably damaging	0.99
IGL01599:Tmem132c	APN	5	127,436,616 (GRCm39)	splice site	probably benign
IGL01750:Tmem132c	APN	5	127,540,023 (GRCm39)	missense	possibly damaging	0.91
IGL01893:Tmem132c	APN	5	127,540,093 (GRCm39)	missense	possibly damaging	0.51
IGL02143:Tmem132c	APN	5	127,640,466 (GRCm39)	missense	probably benign	0.03
IGL02164:Tmem132c	APN	5	127,613,441 (GRCm39)	missense	probably damaging	1.00
IGL02252:Tmem132c	APN	5	127,539,991 (GRCm39)	missense	possibly damaging	0.91
IGL02527:Tmem132c	APN	5	127,436,675 (GRCm39)	missense	possibly damaging	0.95
IGL02584:Tmem132c	APN	5	127,582,063 (GRCm39)	missense	probably damaging	1.00
IGL02878:Tmem132c	APN	5	127,640,154 (GRCm39)	missense	probably damaging	0.99
IGL03065:Tmem132c	APN	5	127,640,688 (GRCm39)	missense	probably damaging	1.00
IGL03120:Tmem132c	APN	5	127,640,448 (GRCm39)	missense	probably benign	0.06
IGL03380:Tmem132c	APN	5	127,613,506 (GRCm39)	missense	probably benign	0.00
IGL03387:Tmem132c	APN	5	127,640,784 (GRCm39)	missense	probably benign	0.00
R0106:Tmem132c	UTSW	5	127,631,733 (GRCm39)	missense	possibly damaging	0.94
R0106:Tmem132c	UTSW	5	127,631,733 (GRCm39)	missense	possibly damaging	0.94
R0415:Tmem132c	UTSW	5	127,640,769 (GRCm39)	missense	probably damaging	1.00
R0607:Tmem132c	UTSW	5	127,640,617 (GRCm39)	nonsense	probably null
R1413:Tmem132c	UTSW	5	127,640,631 (GRCm39)	missense	probably damaging	1.00
R1531:Tmem132c	UTSW	5	127,436,955 (GRCm39)	missense	probably damaging	1.00
R1648:Tmem132c	UTSW	5	127,540,120 (GRCm39)	splice site	probably benign
R2148:Tmem132c	UTSW	5	127,540,026 (GRCm39)	missense	probably damaging	1.00
R2149:Tmem132c	UTSW	5	127,540,026 (GRCm39)	missense	probably damaging	1.00
R2259:Tmem132c	UTSW	5	127,581,988 (GRCm39)	missense	probably benign	0.01
R3853:Tmem132c	UTSW	5	127,436,933 (GRCm39)	missense	probably benign	0.00
R4204:Tmem132c	UTSW	5	127,640,829 (GRCm39)	missense	possibly damaging	0.73
R4543:Tmem132c	UTSW	5	127,582,041 (GRCm39)	missense	probably benign	0.00
R4701:Tmem132c	UTSW	5	127,641,560 (GRCm39)	unclassified	probably benign
R5017:Tmem132c	UTSW	5	127,640,414 (GRCm39)	missense	probably benign	0.13
R5037:Tmem132c	UTSW	5	127,630,199 (GRCm39)	missense	probably benign	0.42
R5327:Tmem132c	UTSW	5	127,640,816 (GRCm39)	missense	possibly damaging	0.50
R5423:Tmem132c	UTSW	5	127,640,907 (GRCm39)	missense	probably benign	0.02
R5548:Tmem132c	UTSW	5	127,628,587 (GRCm39)	nonsense	probably null
R6425:Tmem132c	UTSW	5	127,630,329 (GRCm39)	missense	possibly damaging	0.95
R6717:Tmem132c	UTSW	5	127,641,093 (GRCm39)	missense	possibly damaging	0.93
R6899:Tmem132c	UTSW	5	127,628,744 (GRCm39)	missense	probably damaging	1.00
R7007:Tmem132c	UTSW	5	127,436,679 (GRCm39)	missense	probably damaging	1.00
R7297:Tmem132c	UTSW	5	127,437,281 (GRCm39)	missense	probably benign	0.06
R7326:Tmem132c	UTSW	5	127,641,123 (GRCm39)	missense	possibly damaging	0.51
R7386:Tmem132c	UTSW	5	127,640,990 (GRCm39)	missense	probably benign	0.16
R7504:Tmem132c	UTSW	5	127,631,696 (GRCm39)	missense	probably damaging	1.00
R7640:Tmem132c	UTSW	5	127,640,070 (GRCm39)	missense	probably damaging	1.00
R7718:Tmem132c	UTSW	5	127,640,504 (GRCm39)	missense	probably benign	0.06
R7762:Tmem132c	UTSW	5	127,631,760 (GRCm39)	missense	possibly damaging	0.90
R7818:Tmem132c	UTSW	5	127,641,152 (GRCm39)	makesense	probably null
R8117:Tmem132c	UTSW	5	127,437,176 (GRCm39)	missense	probably benign	0.22
R8425:Tmem132c	UTSW	5	127,641,421 (GRCm39)	missense
R8749:Tmem132c	UTSW	5	127,437,003 (GRCm39)	missense	possibly damaging	0.75
R8771:Tmem132c	UTSW	5	127,437,192 (GRCm39)	missense	probably benign	0.00
R9372:Tmem132c	UTSW	5	127,640,145 (GRCm39)	missense	probably damaging	1.00
X0067:Tmem132c	UTSW	5	127,613,471 (GRCm39)	missense	possibly damaging	0.79
Z1088:Tmem132c	UTSW	5	127,581,985 (GRCm39)	missense	probably benign	0.14

Predicted Primers

PCR Primer
(F):5'- TTTGCGTTCAGAGACAGCC -3'
(R):5'- ATGCGGTGACTATGTCTCCC -3'

Sequencing Primer
(F):5'- CGTTCAGAGACAGCCGGGAG -3'
(R):5'- GGCAGCCAGATGACCACATTG -3'

Posted On

2021-04-30