Mutagenetix > Incidental Mutation

Incidental Mutation 'R8799:Tmem101'

671454

Institutional Source

Beutler Lab

Gene Symbol

Tmem101

Ensembl Gene

ENSMUSG00000020921

Gene Name

transmembrane protein 101

Synonyms

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.141) question?

Stock #

R8799 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

102152546-102156404 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 102153510 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 184 (F184L)

Ref Sequence

ENSEMBL: ENSMUSP00000021296 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021296] [ENSMUST00000055409]

AlphaFold

Q91VP7

Predicted Effect

probably benign
Transcript: ENSMUST00000021296
AA Change: F184L

PolyPhen 2 Score 0.077 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000021296
Gene: ENSMUSG00000020921
AA Change: F184L

Domain	Start	End	E-Value	Type
Pfam:TMEM101	8	256	9.9e-122	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000055409

SMART Domains

Protein: ENSMUSP00000050258
Gene: ENSMUSG00000048217

Domain	Start	End	E-Value	Type
low complexity region	9	48	N/A	INTRINSIC
low complexity region	77	90	N/A	INTRINSIC
Pfam:NAT	349	514	3.5e-50	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

97% (57/59)

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700020L24Rik	T	A	11: 83,440,752	C134S	probably damaging	Het
Acox3	T	G	5: 35,589,708	F156L	probably damaging	Het
Adprhl1	T	C	8: 13,222,474	E1428G	probably benign	Het
Arhgap40	A	T	2: 158,512,838	M15L	probably benign	Het
Bahcc1	C	A	11: 120,286,347	F2020L	probably damaging	Het
BC024063	A	T	10: 82,109,518	H324L	probably benign	Het
Ccdc40	C	A	11: 119,264,466	S1177R	probably benign	Het
Ccne2	A	T	4: 11,201,355	R294S	probably benign	Het
Cib1	A	C	7: 80,232,543	S6R	probably damaging	Het
Csnk2a1	A	G	2: 152,257,966	E114G	probably damaging	Het
Cxxc1	A	G	18: 74,221,057		probably benign	Het
Decr2	A	C	17: 26,088,937	I47M	possibly damaging	Het
Dmxl2	A	G	9: 54,419,743		probably null	Het
Dse	G	A	10: 34,184,153		probably benign	Het
Eml6	A	G	11: 29,758,981	V1480A	probably benign	Het
Erap1	A	G	13: 74,657,636	I165M	probably benign	Het
Fan1	G	T	7: 64,366,658	Q573K	probably damaging	Het
Fndc3a	G	A	14: 72,556,515	T906I	probably benign	Het
Gm21915	A	C	9: 40,670,710	H33P	possibly damaging	Het
Idh1	CA	CAA	1: 65,165,188		probably null	Het
Lama3	G	T	18: 12,490,943	Q1384H	probably damaging	Het
Lrrc34	C	T	3: 30,624,830	E373K	probably benign	Het
Ly75	A	G	2: 60,348,441	F677L	probably damaging	Het
Megf11	A	G	9: 64,681,391	D493G	probably benign	Het
Mta3	A	T	17: 83,788,940	I348F	possibly damaging	Het
Myo1d	A	T	11: 80,684,379	F170L	probably damaging	Het
Nfkbia	A	G	12: 55,492,298		probably benign	Het
Nfx1	A	T	4: 41,023,727	E1045D	probably damaging	Het
Npffr2	T	G	5: 89,583,318	V369G	probably benign	Het
Olfr1136	G	T	2: 87,693,713	H56Q	possibly damaging	Het
Olfr1312	A	T	2: 112,042,183	I283N	probably damaging	Het
Olfr141	A	T	2: 86,806,231	M256K	probably damaging	Het
Olfr301	T	C	7: 86,412,646	C95R	probably damaging	Het
Olfr984	A	T	9: 40,100,689	M267K	possibly damaging	Het
Otoa	G	A	7: 121,092,671	E41K	possibly damaging	Het
Podxl	A	G	6: 31,524,465	V385A	probably damaging	Het
Ppp4r4	C	T	12: 103,601,364	T673M	possibly damaging	Het
Rbm20	G	T	19: 53,832,689	C525F	probably damaging	Het
Rnf17	A	T	14: 56,500,429	E1222D	probably damaging	Het
Ros1	G	A	10: 52,046,047	A2331V	probably benign	Het
Rrs1	T	A	1: 9,545,594	I24N	probably damaging	Het
Sec11a	A	G	7: 80,935,102	V29A	possibly damaging	Het
Shisa3	T	A	5: 67,611,406	Y216*	probably null	Het
Slc22a2	A	T	17: 12,612,538	Y405F	probably benign	Het
Slc2a12	A	T	10: 22,692,206	M511L	possibly damaging	Het
Slc38a9	T	A	13: 112,703,602	H338Q	probably damaging	Het
Slco1c1	T	G	6: 141,559,805	S486A	probably benign	Het
Slit2	T	C	5: 48,304,182	S1524P	possibly damaging	Het
Spata31d1c	T	C	13: 65,036,326	S561P	possibly damaging	Het
Stac3	T	A	10: 127,504,912	M182K	probably damaging	Het
Stt3b	T	C	9: 115,248,617	E689G	probably damaging	Het
Suclg1	A	G	6: 73,271,108	K271E	probably benign	Het
Tas1r1	A	G	4: 152,032,251	Y309H	probably benign	Het
Tbc1d5	TTGCTGCTGCTGCTGCTG	TTGCTGCTGCTGCTGCTGCTG	17: 50,799,934		probably benign	Het
Tbc1d5	TGC	TGCGGC	17: 50,799,935		probably benign	Het
Tbc1d5	TGC	TGCCGC	17: 50,799,941		probably benign	Het
Tbc1d5	TG	TGCGG	17: 50,799,950		probably benign	Het
Zfp474	A	G	18: 52,639,094	Q273R	probably benign	Het
Zfyve28	A	G	5: 34,233,326	L197P	probably damaging	Het

Other mutations in Tmem101

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01085:Tmem101	APN	11	102154660	missense	probably damaging	0.99
IGL01096:Tmem101	APN	11	102154552	splice site	probably null
IGL01593:Tmem101	APN	11	102155878	missense	probably damaging	1.00
IGL01814:Tmem101	APN	11	102153458	missense	possibly damaging	0.58
IGL02451:Tmem101	APN	11	102153293	missense	probably damaging	1.00
IGL03238:Tmem101	APN	11	102155785	missense	probably damaging	1.00
R0462:Tmem101	UTSW	11	102155867	missense	probably benign	0.08
R0848:Tmem101	UTSW	11	102155866	missense	possibly damaging	0.65
R1465:Tmem101	UTSW	11	102153329	missense	probably damaging	0.97
R1465:Tmem101	UTSW	11	102153329	missense	probably damaging	0.97
R1722:Tmem101	UTSW	11	102154693	missense	probably damaging	1.00
R1928:Tmem101	UTSW	11	102153396	missense	probably benign
R2082:Tmem101	UTSW	11	102153377	missense	probably benign	0.17
R4577:Tmem101	UTSW	11	102155837	missense	possibly damaging	0.48
R4724:Tmem101	UTSW	11	102153443	missense	probably benign	0.32
R4729:Tmem101	UTSW	11	102156329	missense	probably benign	0.25
R5146:Tmem101	UTSW	11	102154624	missense	probably benign
R5184:Tmem101	UTSW	11	102156233	missense	possibly damaging	0.78
R7381:Tmem101	UTSW	11	102153350	missense	possibly damaging	0.91
R9612:Tmem101	UTSW	11	102153368	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTAGCTTCATCTGGTTCCAG -3'
(R):5'- AGACACTCATCTGCTGCAGTC -3'

Sequencing Primer
(F):5'- ATCTGGTTCCAGAACTCGACG -3'
(R):5'- CTTGAACTCAGGACCTCTGGAAG -3'

Posted On

2021-04-30