Mutagenetix > Incidental Mutation

Incidental Mutation 'R8762:Coro7'

671873

Institutional Source

Beutler Lab

Gene Symbol

Coro7

Ensembl Gene

ENSMUSG00000039637

Gene Name

coronin 7

Synonyms

0610011B16Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R8762 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

4626133-4679777 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 4634339 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glycine at position 410 (V410G)

Ref Sequence

ENSEMBL: ENSMUSP00000048489 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000038552] [ENSMUST00000135823]

AlphaFold

Q9D2V7

Predicted Effect

probably benign
Transcript: ENSMUST00000038552
AA Change: V410G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000048489
Gene: ENSMUSG00000039637
AA Change: V410G

Domain	Start	End	E-Value	Type
DUF1899	3	64	6.41e-15	SMART
WD40	66	106	1.83e-7	SMART
WD40	115	154	4.13e0	SMART
WD40	157	196	1.78e-5	SMART
DUF1900	251	385	4.49e-60	SMART
low complexity region	427	456	N/A	INTRINSIC
DUF1899	463	528	1.2e-19	SMART
WD40	531	570	3.64e-2	SMART
WD40	580	620	8.55e-8	SMART
WD40	623	662	1.16e-9	SMART
low complexity region	667	679	N/A	INTRINSIC
DUF1900	718	854	6.69e-68	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000135823

SMART Domains

Protein: ENSMUSP00000118310
Gene: ENSMUSG00000039637

Domain	Start	End	E-Value	Type
DUF1899	3	64	6.41e-15	SMART
WD40	66	106	1.83e-7	SMART
WD40	115	154	4.13e0	SMART
WD40	157	196	1.78e-5	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

100% (66/66)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele are viable and overtly normal but exhibit disruption of the Golgi apparatus. Mutant fibroblasts show increased cell spreading and cellular F-actin content, increased cell polarization and migration, and enhanced wound healing in a scratch-wound assay. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5830473C10Rik	A	G	5: 90,566,602	N157S	probably benign	Het
Aarsd1	A	G	11: 101,410,400	V272A	probably benign	Het
Acbd6	G	A	1: 155,686,960	E236K	probably damaging	Het
Actr1b	T	C	1: 36,709,828	N9S	probably benign	Het
Adam17	A	T	12: 21,351,594	D133E	probably benign	Het
Adam6b	G	A	12: 113,489,607	V15M	probably damaging	Het
Adam9	T	A	8: 24,967,219	N631I	probably damaging	Het
Amy2a1	A	T	3: 113,531,627		probably benign	Het
Aplnr	A	T	2: 85,137,171	Y180F	probably benign	Het
Arhgef7	T	C	8: 11,781,216	V105A	probably benign	Het
Bambi	G	A	18: 3,511,277	D33N	probably damaging	Het
Bicc1	G	A	10: 70,943,386	T724I	probably benign	Het
Brd2	T	A	17: 34,116,960	Q93L	probably damaging	Het
C130060K24Rik	T	C	6: 65,447,409	V182A	probably benign	Het
Cacnb2	A	G	2: 14,967,948	D222G	possibly damaging	Het
Ccdc155	G	T	7: 45,196,057	D152E	probably damaging	Het
Cdh1	G	T	8: 106,659,704	D420Y	probably damaging	Het
Cep162	T	C	9: 87,227,261	S430G	probably benign	Het
Cfap161	T	A	7: 83,794,074	R27S	possibly damaging	Het
Chek1	C	T	9: 36,718,340	A237T	probably benign	Het
Chrna3	T	A	9: 55,015,711	K271M	probably damaging	Het
Crmp1	C	A	5: 37,284,096	N507K	probably damaging	Het
Crocc	T	C	4: 141,034,058	R755G	possibly damaging	Het
Disc1	A	G	8: 125,155,057	D577G	probably damaging	Het
Dnah6	A	G	6: 73,179,828	Y649H	possibly damaging	Het
Flywch1	A	G	17: 23,756,757	S504P	probably damaging	Het
Fpr1	A	T	17: 17,877,589	V46D	probably damaging	Het
Fpr-rs7	C	T	17: 20,113,527	V234M	probably benign	Het
Ighv5-16	T	C	12: 113,838,668	N71D	probably benign	Het
Kdm3b	A	G	18: 34,804,104	M480V	probably benign	Het
L3mbtl3	T	A	10: 26,276,223	D825V	probably damaging	Het
Lef1	T	A	3: 131,194,717	M311K	probably damaging	Het
Magi1	T	A	6: 93,815,808	R150*	probably null	Het
Magi3	C	T	3: 104,050,853	V639I	probably damaging	Het
Mocos	A	T	18: 24,679,497	R483W	probably damaging	Het
Mrps11	G	T	7: 78,788,739	V80F	possibly damaging	Het
Myh2	A	T	11: 67,193,752	R1706W	probably damaging	Het
Neurod6	T	A	6: 55,679,243	L136F	probably damaging	Het
Nfe2l1	A	T	11: 96,820,480	Y308N	probably damaging	Het
Nptn	T	A	9: 58,618,622		probably benign	Het
Olfr1002	A	T	2: 85,647,690	S210R	probably damaging	Het
Olfr388-ps1	G	T	11: 73,724,481	T181N	unknown	Het
Oscar	A	G	7: 3,610,901	S227P	probably benign	Het
Phgdh	A	C	3: 98,339,708	I42R	possibly damaging	Het
Pla2g4a	C	T	1: 149,886,184	G174D	probably benign	Het
Plcd4	A	G	1: 74,552,054	T203A	possibly damaging	Het
Plk3	ACACTCAC	ACAC	4: 117,131,893		probably benign	Het
Plppr4	A	T	3: 117,325,833	V309D	probably damaging	Het
Pltp	T	C	2: 164,844,732	K324E	possibly damaging	Het
Pml	C	T	9: 58,247,065	R175H	probably damaging	Het
Psg20	C	A	7: 18,674,632	V388F	probably benign	Het
Rabgef1	A	G	5: 130,208,716	D209G	possibly damaging	Het
Rif1	A	T	2: 52,111,730	N90I		Het
Sec31a	T	C	5: 100,378,829	H57R		Het
Sidt1	T	C	16: 44,332,344	N62S	probably benign	Het
Slc18a2	T	A	19: 59,272,923	M172K	probably benign	Het
Slc34a3	T	C	2: 25,230,991	T362A	probably benign	Het
Smtn	T	G	11: 3,526,407	D158A	probably benign	Het
Spen	C	T	4: 141,472,950	V2789M	probably damaging	Het
Taf2	A	T	15: 55,047,453	N608K	probably benign	Het
Tctn3	A	G	19: 40,607,192	V383A	unknown	Het
Themis3	A	T	17: 66,559,681	M188K	probably benign	Het
Trpv5	A	G	6: 41,675,379	V124A	probably benign	Het
Tshr	G	A	12: 91,537,550	V421M	probably damaging	Het
Ttn	T	A	2: 76,709,082	D34520V	probably benign	Het
Ttn	A	C	2: 76,777,950	Y17876*	probably null	Het
Zrsr1	T	C	11: 22,973,694	L156P	probably benign	Het

Other mutations in Coro7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00428:Coro7	APN	16	4634636	missense	possibly damaging	0.83
IGL00885:Coro7	APN	16	4635026	missense	probably benign	0.00
IGL02944:Coro7	APN	16	4635412	missense	probably benign	0.14
IGL03104:Coro7	APN	16	4629126	missense	probably damaging	1.00
IGL03153:Coro7	APN	16	4635382	critical splice donor site	probably null
R0022:Coro7	UTSW	16	4633304	missense	probably benign	0.01
R0022:Coro7	UTSW	16	4633304	missense	probably benign	0.01
R0071:Coro7	UTSW	16	4670527	missense	probably damaging	1.00
R0071:Coro7	UTSW	16	4670527	missense	probably damaging	1.00
R0080:Coro7	UTSW	16	4630464	missense	probably damaging	1.00
R0193:Coro7	UTSW	16	4627504	unclassified	probably benign
R0242:Coro7	UTSW	16	4630178	splice site	probably benign
R0318:Coro7	UTSW	16	4675807	missense	probably benign	0.09
R0554:Coro7	UTSW	16	4632257	missense	possibly damaging	0.63
R0666:Coro7	UTSW	16	4631911	missense	possibly damaging	0.70
R0835:Coro7	UTSW	16	4632254	missense	probably benign	0.12
R0968:Coro7	UTSW	16	4670055	splice site	probably benign
R1670:Coro7	UTSW	16	4628233	missense	possibly damaging	0.76
R1709:Coro7	UTSW	16	4634441	splice site	probably null
R1848:Coro7	UTSW	16	4630434	missense	probably damaging	0.99
R1884:Coro7	UTSW	16	4628819	unclassified	probably benign
R1935:Coro7	UTSW	16	4628732	missense	probably benign
R1937:Coro7	UTSW	16	4628732	missense	probably benign
R1939:Coro7	UTSW	16	4628732	missense	probably benign
R1967:Coro7	UTSW	16	4634889	missense	probably damaging	1.00
R1969:Coro7	UTSW	16	4633756	missense	probably benign	0.19
R1970:Coro7	UTSW	16	4633756	missense	probably benign	0.19
R3034:Coro7	UTSW	16	4632291	missense	probably damaging	0.99
R4638:Coro7	UTSW	16	4632287	missense	probably damaging	0.96
R4710:Coro7	UTSW	16	4634933	intron	probably benign
R4723:Coro7	UTSW	16	4631994	missense	probably benign	0.00
R4789:Coro7	UTSW	16	4628221	missense	probably damaging	1.00
R5493:Coro7	UTSW	16	4632487	missense	probably damaging	0.99
R5619:Coro7	UTSW	16	4676935	critical splice donor site	probably null
R5756:Coro7	UTSW	16	4632284	missense	probably damaging	0.97
R5974:Coro7	UTSW	16	4631889	missense	possibly damaging	0.83
R6010:Coro7	UTSW	16	4669956	missense	possibly damaging	0.68
R6038:Coro7	UTSW	16	4679550	critical splice donor site	probably null
R6038:Coro7	UTSW	16	4679550	critical splice donor site	probably null
R6906:Coro7	UTSW	16	4633304	missense	probably benign	0.00
R6925:Coro7	UTSW	16	4628674	critical splice donor site	probably null
R7069:Coro7	UTSW	16	4679611	start codon destroyed	probably damaging	0.99
R7326:Coro7	UTSW	16	4632048	missense	probably damaging	0.96
R7421:Coro7	UTSW	16	4668751	missense	probably benign	0.19
R7521:Coro7	UTSW	16	4631482	missense	probably benign	0.00
R7773:Coro7	UTSW	16	4632006	missense	probably damaging	1.00
R7846:Coro7	UTSW	16	4670536	missense	probably damaging	1.00
R8240:Coro7	UTSW	16	4668796	missense	probably damaging	0.96
R8726:Coro7	UTSW	16	4668755	missense	possibly damaging	0.95
R9383:Coro7	UTSW	16	4635024	missense	probably damaging	1.00
R9451:Coro7	UTSW	16	4670538	missense	probably damaging	1.00
R9553:Coro7	UTSW	16	4668760	missense	possibly damaging	0.55

Predicted Primers

PCR Primer
(F):5'- TTTAACACCTGCTCAGCTTAGC -3'
(R):5'- GGAGCTCTCATGTGGTTCTCTC -3'

Sequencing Primer
(F):5'- GCTCAGCTTAGCACACAGTG -3'
(R):5'- CAATCTCATGTCTACCCTCAATAGG -3'

Posted On

2021-04-30