Mutagenetix > Incidental Mutation

Incidental Mutation 'K3955:Vmn1r84'

67231

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r84

Ensembl Gene

ENSMUSG00000066803

Gene Name

vomeronasal 1 receptor 84

Synonyms

V1rg11

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.049) question?

Stock #

K3955 (G3) of strain 706

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

12095735-12096691 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 12095884 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 270 (V270M)

Ref Sequence

ENSEMBL: ENSMUSP00000083405 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086228] [ENSMUST00000226408] [ENSMUST00000227427] [ENSMUST00000228653]

AlphaFold

Q8R284

Predicted Effect

probably damaging
Transcript: ENSMUST00000086228
AA Change: V270M

PolyPhen 2 Score 0.979 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000083405
Gene: ENSMUSG00000066803
AA Change: V270M

Domain	Start	End	E-Value	Type
Pfam:TAS2R	16	312	3.7e-11	PFAM
Pfam:V1R	45	308	1.9e-22	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000177845

Predicted Effect

probably damaging
Transcript: ENSMUST00000226408
AA Change: V258M

PolyPhen 2 Score 0.974 (Sensitivity: 0.76; Specificity: 0.96)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000226811

Predicted Effect

probably damaging
Transcript: ENSMUST00000227427
AA Change: V258M

PolyPhen 2 Score 0.974 (Sensitivity: 0.76; Specificity: 0.96)

Predicted Effect

probably damaging
Transcript: ENSMUST00000228653
AA Change: V258M

PolyPhen 2 Score 0.974 (Sensitivity: 0.76; Specificity: 0.96)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000228741

Meta Mutation Damage Score

0.1998

Coding Region Coverage

1x: 99.5%
3x: 98.9%
10x: 97.4%
20x: 94.9%

Validation Efficiency

100% (42/42)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agap1	G	A	1: 89,815,326 (GRCm39)	R738H	probably damaging	Het
Arhgap28	T	C	17: 68,311,001 (GRCm39)	E2G	probably damaging	Het
Atad2b	C	A	12: 5,004,536 (GRCm39)		probably benign	Het
Atmin	T	A	8: 117,683,775 (GRCm39)	C478*	probably null	Het
Calr	T	C	8: 85,572,902 (GRCm39)	Y57C	probably damaging	Het
Cdh13	G	A	8: 119,401,843 (GRCm39)	V82M	probably damaging	Het
Ces3a	A	T	8: 105,777,259 (GRCm39)		probably benign	Het
Dmbt1	T	C	7: 130,721,293 (GRCm39)	Y1854H	probably damaging	Het
Dnah1	T	A	14: 30,988,416 (GRCm39)	M3429L	probably benign	Het
Dscam	A	G	16: 96,474,887 (GRCm39)	F1225S	probably benign	Het
E030025P04Rik	G	A	11: 109,034,778 (GRCm39)	P37S	unknown	Het
Eral1	A	T	11: 77,966,847 (GRCm39)	D189E	probably damaging	Het
Fbxw14	G	T	9: 109,105,313 (GRCm39)	P284Q	possibly damaging	Het
Fcrl6	A	G	1: 172,425,251 (GRCm39)	V260A	probably benign	Het
Fezf2	A	T	14: 12,345,097 (GRCm38)	F30Y	probably damaging	Het
Gjb4	A	T	4: 127,245,293 (GRCm39)	V216D	probably benign	Het
Gm9758	G	A	5: 14,963,522 (GRCm39)		probably benign	Het
Gm9758	C	G	5: 14,963,553 (GRCm39)	V92L	probably benign	Het
Gmps	A	C	3: 63,908,954 (GRCm39)	R485S	probably damaging	Het
Gtdc1	C	T	2: 44,642,233 (GRCm39)		probably null	Het
H2-Ob	C	T	17: 34,460,158 (GRCm39)	R19C	probably damaging	Het
Lars2	T	C	9: 123,206,842 (GRCm39)	V103A	probably damaging	Het
Mtrex	A	C	13: 113,047,513 (GRCm39)	Y277*	probably null	Het
Ndnf	G	A	6: 65,678,413 (GRCm39)		probably benign	Het
Nectin1	A	G	9: 43,703,375 (GRCm39)	Y211C	probably damaging	Het
Notch4	C	T	17: 34,787,436 (GRCm39)	T332I	probably damaging	Het
Or13c25	A	G	4: 52,911,081 (GRCm39)	F238L	probably damaging	Het
Or8g28	A	C	9: 39,169,926 (GRCm39)	L14W	probably damaging	Het
Or8g53	A	G	9: 39,683,469 (GRCm39)	I209T	probably benign	Het
Paf1	T	C	7: 28,096,350 (GRCm39)		probably null	Het
Pcdhb1	G	T	18: 37,399,026 (GRCm39)	G326C	probably damaging	Het
Plcl1	A	G	1: 55,737,098 (GRCm39)	Y813C	possibly damaging	Het
Pramel25	T	C	4: 143,521,710 (GRCm39)	I442T	possibly damaging	Het
Prkcq	T	C	2: 11,251,604 (GRCm39)		probably benign	Het
Proser3	G	T	7: 30,242,924 (GRCm39)	P218T	probably damaging	Het
Rccd1	G	A	7: 79,970,419 (GRCm39)	S66F	probably benign	Het
Recql	G	T	6: 142,323,932 (GRCm39)	S54*	probably null	Het
Samd15	G	T	12: 87,247,534 (GRCm39)	G73V	probably benign	Het
Siglec1	T	C	2: 130,923,359 (GRCm39)	N462S	probably benign	Het
Syne2	G	T	12: 75,977,439 (GRCm39)	A1296S	probably damaging	Het
Tlk1	T	C	2: 70,552,045 (GRCm39)	E542G	possibly damaging	Het
Tnks1bp1	C	T	2: 84,892,755 (GRCm39)	T232I	probably benign	Het
Tnrc6c	T	A	11: 117,651,564 (GRCm39)	Y1696N	probably damaging	Het
Uggt1	A	G	1: 36,201,434 (GRCm39)	I1102T	probably benign	Het
Wasf1	C	T	10: 40,812,191 (GRCm39)	P327S	unknown	Het

Other mutations in Vmn1r84

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02505:Vmn1r84	APN	7	12,096,346 (GRCm39)	missense	probably damaging	1.00
R0360:Vmn1r84	UTSW	7	12,095,799 (GRCm39)	missense	probably damaging	1.00
R0399:Vmn1r84	UTSW	7	12,095,794 (GRCm39)	missense	probably benign	0.26
R0697:Vmn1r84	UTSW	7	12,096,690 (GRCm39)	start codon destroyed	probably null	0.53
R1613:Vmn1r84	UTSW	7	12,096,460 (GRCm39)	missense	possibly damaging	0.80
R1636:Vmn1r84	UTSW	7	12,096,522 (GRCm39)	missense	probably benign	0.12
R1872:Vmn1r84	UTSW	7	12,096,555 (GRCm39)	missense	probably benign	0.17
R4022:Vmn1r84	UTSW	7	12,095,857 (GRCm39)	missense	probably benign
R4923:Vmn1r84	UTSW	7	12,095,743 (GRCm39)	missense	probably benign
R5189:Vmn1r84	UTSW	7	12,096,385 (GRCm39)	missense	probably benign	0.10
R5275:Vmn1r84	UTSW	7	12,095,741 (GRCm39)	missense	probably benign
R5721:Vmn1r84	UTSW	7	12,096,153 (GRCm39)	missense	probably damaging	1.00
R6151:Vmn1r84	UTSW	7	12,095,841 (GRCm39)	missense	possibly damaging	0.72
R7261:Vmn1r84	UTSW	7	12,096,069 (GRCm39)	missense	probably damaging	1.00
R7513:Vmn1r84	UTSW	7	12,095,994 (GRCm39)	missense	probably benign	0.00
R7573:Vmn1r84	UTSW	7	12,095,787 (GRCm39)	missense	probably benign	0.17
R8039:Vmn1r84	UTSW	7	12,095,935 (GRCm39)	missense	possibly damaging	0.67
R8814:Vmn1r84	UTSW	7	12,096,385 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn1r84	UTSW	7	12,096,586 (GRCm39)	missense	possibly damaging	0.92
Z1177:Vmn1r84	UTSW	7	12,096,439 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GTGCCTACACAGCAGTTCATACCTC -3'
(R):5'- TTCAGCAATGGTGTTACTGGCCTAC -3'

Sequencing Primer
(F):5'- AGTTCATACCTCAAGACCTGGTTG -3'
(R):5'- CAATGGTGTTACTGGCCTACATTTG -3'

Posted On

2013-09-03