Mutagenetix > Incidental Mutation

Incidental Mutation 'R8824:Mrgpra9'

673327

Institutional Source

Beutler Lab

Gene Symbol

Mrgpra9

Ensembl Gene

ENSMUSG00000074111

Gene Name

MAS-related GPR, member A9

Synonyms

EG668725, MrgA9

MMRRC Submission

068657-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.054) question?

Stock #

R8824 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

47234861-47252848 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 47235293 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 209 (C209S)

Ref Sequence

ENSEMBL: ENSMUSP00000136396 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098436] [ENSMUST00000179005]

AlphaFold

A0A140T8U8

Predicted Effect

probably benign
Transcript: ENSMUST00000098436
AA Change: C208S

PolyPhen 2 Score 0.060 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000096035
Gene: ENSMUSG00000074111
AA Change: C208S

Domain	Start	End	E-Value	Type
low complexity region	37	48	N/A	INTRINSIC
Pfam:7tm_1	56	225	1.5e-11	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000179005
AA Change: C209S

PolyPhen 2 Score 0.060 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000136396
Gene: ENSMUSG00000074111
AA Change: C209S

Domain	Start	End	E-Value	Type
Pfam:7tm_1	12	178	3.4e-9	PFAM

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.5%

Validation Efficiency

100% (63/63)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Accsl	A	T	2: 93,862,850 (GRCm38)	V260D	probably damaging	Het
Adora2a	G	A	10: 75,326,179 (GRCm38)	A51T	probably damaging	Het
Afg1l	G	A	10: 42,438,387 (GRCm38)	P128S	possibly damaging	Het
Ago4	C	A	4: 126,507,184 (GRCm38)	V623L	probably benign	Het
Akap11	A	T	14: 78,516,347 (GRCm38)	N112K		Het
C1ra	A	T	6: 124,517,695 (GRCm38)	I306F	probably damaging	Het
Ccdc73	A	G	2: 104,991,877 (GRCm38)	N724D	possibly damaging	Het
Cd248	A	G	19: 5,069,617 (GRCm38)	I498V	probably benign	Het
Clrn1	A	T	3: 58,884,893 (GRCm38)	S50T	probably benign	Het
Col7a1	A	G	9: 108,967,025 (GRCm38)	K1553R	unknown	Het
Cxcr6	A	C	9: 123,810,941 (GRCm38)	T343P	probably benign	Het
Cyp11b2	T	A	15: 74,856,065 (GRCm38)	Q56L	probably damaging	Het
Dip2a	A	G	10: 76,278,486 (GRCm38)		probably null	Het
Dnmbp	A	G	19: 43,849,837 (GRCm38)	V742A	probably benign	Het
Dusp16	A	T	6: 134,739,769 (GRCm38)	S192T	probably benign	Het
Ehbp1	A	T	11: 22,232,053 (GRCm38)	D87E	probably damaging	Het
Fgl1	A	G	8: 41,199,711 (GRCm38)	V150A	probably benign	Het
Flt3	T	C	5: 147,334,863 (GRCm38)	D873G	probably damaging	Het
Gart	T	C	16: 91,630,703 (GRCm38)	D469G	possibly damaging	Het
Gm28168	T	G	1: 117,947,895 (GRCm38)	S85A	probably benign	Het
Golgb1	A	G	16: 36,915,689 (GRCm38)	D1807G	probably benign	Het
Grm8	A	G	6: 27,761,352 (GRCm38)	L291S	probably damaging	Het
Gucy2d	C	T	7: 98,443,469 (GRCm38)	P18S	possibly damaging	Het
Ifna15	C	T	4: 88,557,761 (GRCm38)	C162Y	probably damaging	Het
Iqub	C	A	6: 24,479,308 (GRCm38)	E412*	probably null	Het
Krt4	T	A	15: 101,920,642 (GRCm38)	D312V		Het
Krtap26-1	A	T	16: 88,647,415 (GRCm38)	I106N	probably damaging	Het
Krtap26-1	T	C	16: 88,647,436 (GRCm38)	Y99C	probably damaging	Het
Lipn	T	C	19: 34,084,716 (GRCm38)	I357T	probably benign	Het
Lrrc14b	A	G	13: 74,363,949 (GRCm38)	L4P	probably damaging	Het
Myh7b	A	G	2: 155,630,381 (GRCm38)	N1291D	probably benign	Het
Myo5a	A	G	9: 75,167,046 (GRCm38)	T746A	probably damaging	Het
Myom2	A	C	8: 15,114,169 (GRCm38)	E1021D	possibly damaging	Het
Ncoa2	C	T	1: 13,177,185 (GRCm38)	R338H	probably benign	Het
Ncor2	A	G	5: 125,118,757 (GRCm38)	F91L		Het
Neb	C	G	2: 52,216,911 (GRCm38)	A4407P	probably damaging	Het
Olfr1135	T	C	2: 87,671,960 (GRCm38)	M136V	possibly damaging	Het
Olfr1272	A	G	2: 90,296,012 (GRCm38)	I283T	probably damaging	Het
Olfr347	A	T	2: 36,735,191 (GRCm38)	Y290F	probably damaging	Het
Olfr502	T	G	7: 108,523,143 (GRCm38)	Y269S	probably benign	Het
Olfr74	A	G	2: 87,974,003 (GRCm38)	F221L	probably benign	Het
Olfr819	C	T	10: 129,965,792 (GRCm38)	V297M	probably damaging	Het
Peg10	T	TCCC	6: 4,756,451 (GRCm38)		probably benign	Het
Piwil4	C	A	9: 14,727,475 (GRCm38)	K298N	probably benign	Het
Prkcz	G	A	4: 155,344,828 (GRCm38)		probably benign	Het
Prkg2	G	A	5: 98,942,208 (GRCm38)	P691L	possibly damaging	Het
Ptprc	T	A	1: 138,113,708 (GRCm38)	K89*	probably null	Het
Rapgef3	C	T	15: 97,766,908 (GRCm38)	A25T	probably benign	Het
Rreb1	C	T	13: 37,930,516 (GRCm38)	T617I	probably damaging	Het
Rsph1	A	C	17: 31,273,376 (GRCm38)	V72G	possibly damaging	Het
Shc2	A	G	10: 79,637,702 (GRCm38)	V50A	probably benign	Het
Slc38a9	G	A	13: 112,701,487 (GRCm38)	R262H	probably benign	Het
Slco6c1	T	A	1: 97,128,159 (GRCm38)	N6Y	possibly damaging	Het
Smarca5	A	G	8: 80,705,332 (GRCm38)	F886L	probably benign	Het
Tas1r2	A	G	4: 139,653,763 (GRCm38)		probably benign	Het
Tnrc6c	T	A	11: 117,739,854 (GRCm38)		probably benign	Het
Trim30b	T	A	7: 104,357,906 (GRCm38)		probably benign	Het
Trim55	T	C	3: 19,672,962 (GRCm38)	S398P	probably benign	Het
Ttc39c	T	A	18: 12,686,946 (GRCm38)		probably benign	Het
Ubxn10	A	G	4: 138,735,867 (GRCm38)		probably null	Het
Usf3	A	G	16: 44,215,613 (GRCm38)	N152S	probably benign	Het
Vps13b	T	A	15: 35,533,299 (GRCm38)	V839E	probably damaging	Het
Zeb1	T	A	18: 5,748,680 (GRCm38)		probably benign	Het
Zfp780b	T	C	7: 27,963,468 (GRCm38)	Y554C	probably benign	Het
Zhx2	C	T	15: 57,821,280 (GRCm38)	T15I	probably damaging	Het

Other mutations in Mrgpra9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Mrgpra9	APN	7	47,235,091 (GRCm38)	missense	possibly damaging	0.85
IGL00575:Mrgpra9	APN	7	47,235,305 (GRCm38)	missense	possibly damaging	0.76
IGL01649:Mrgpra9	APN	7	47,235,152 (GRCm38)	missense	probably benign	0.22
IGL03207:Mrgpra9	APN	7	47,235,637 (GRCm38)	missense	possibly damaging	0.68
R0388:Mrgpra9	UTSW	7	47,252,794 (GRCm38)	start codon destroyed	probably null	0.08
R0972:Mrgpra9	UTSW	7	47,235,455 (GRCm38)	missense	probably damaging	0.99
R1270:Mrgpra9	UTSW	7	47,252,783 (GRCm38)	critical splice donor site	probably null
R1381:Mrgpra9	UTSW	7	47,235,302 (GRCm38)	missense	possibly damaging	0.75
R1403:Mrgpra9	UTSW	7	47,235,638 (GRCm38)	missense	probably benign	0.24
R1403:Mrgpra9	UTSW	7	47,235,638 (GRCm38)	missense	probably benign	0.24
R1448:Mrgpra9	UTSW	7	47,235,813 (GRCm38)	missense	probably benign	0.02
R2045:Mrgpra9	UTSW	7	47,235,835 (GRCm38)	missense	probably benign
R2144:Mrgpra9	UTSW	7	47,235,463 (GRCm38)	missense	probably benign	0.31
R2187:Mrgpra9	UTSW	7	47,235,049 (GRCm38)	missense	probably damaging	1.00
R2507:Mrgpra9	UTSW	7	47,235,494 (GRCm38)	missense	possibly damaging	0.63
R2913:Mrgpra9	UTSW	7	47,235,080 (GRCm38)	missense	probably benign
R3810:Mrgpra9	UTSW	7	47,235,779 (GRCm38)	missense	probably damaging	0.98
R4177:Mrgpra9	UTSW	7	47,235,554 (GRCm38)	missense	probably damaging	1.00
R4521:Mrgpra9	UTSW	7	47,235,190 (GRCm38)	missense	probably damaging	1.00
R4781:Mrgpra9	UTSW	7	47,235,047 (GRCm38)	missense	possibly damaging	0.88
R4926:Mrgpra9	UTSW	7	47,235,011 (GRCm38)	missense	possibly damaging	0.62
R6469:Mrgpra9	UTSW	7	47,235,106 (GRCm38)	missense	probably benign	0.02
R6505:Mrgpra9	UTSW	7	47,235,136 (GRCm38)	missense	probably benign	0.00
R6724:Mrgpra9	UTSW	7	47,235,038 (GRCm38)	missense	probably damaging	1.00
R7398:Mrgpra9	UTSW	7	47,235,637 (GRCm38)	missense	possibly damaging	0.68
R8737:Mrgpra9	UTSW	7	47,235,876 (GRCm38)	missense	probably benign	0.00
R8881:Mrgpra9	UTSW	7	47,235,494 (GRCm38)	missense	possibly damaging	0.63
R9320:Mrgpra9	UTSW	7	47,235,644 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CAACCGATGCCTGAATGAGC -3'
(R):5'- AGAACACACATCAACTGTCATGTG -3'

Sequencing Primer
(F):5'- TAGCACTCAGGACAGTTGATGC -3'
(R):5'- ACACATCAACTGTCATGTGTGCTG -3'

Posted On

2021-07-15