Mutagenetix > Incidental Mutation

Incidental Mutation 'R8824:Mrgpra9'

673327

Institutional Source

Beutler Lab

Gene Symbol

Mrgpra9

Ensembl Gene

ENSMUSG00000074111

Gene Name

MAS-related GPR, member A9

Synonyms

MrgA9, EG668725

MMRRC Submission

068657-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R8824 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

46884667-46902627 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 46885041 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 209 (C209S)

Ref Sequence

ENSEMBL: ENSMUSP00000136396 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098436] [ENSMUST00000179005]

AlphaFold

A0A140T8U8

Predicted Effect

probably benign
Transcript: ENSMUST00000098436
AA Change: C208S

PolyPhen 2 Score 0.060 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000096035
Gene: ENSMUSG00000074111
AA Change: C208S

Domain	Start	End	E-Value	Type
low complexity region	37	48	N/A	INTRINSIC
Pfam:7tm_1	56	225	1.5e-11	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000179005
AA Change: C209S

PolyPhen 2 Score 0.060 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000136396
Gene: ENSMUSG00000074111
AA Change: C209S

Domain	Start	End	E-Value	Type
Pfam:7tm_1	12	178	3.4e-9	PFAM

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.5%

Validation Efficiency

100% (63/63)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Accsl	A	T	2: 93,693,195 (GRCm39)	V260D	probably damaging	Het
Adora2a	G	A	10: 75,162,013 (GRCm39)	A51T	probably damaging	Het
Afg1l	G	A	10: 42,314,383 (GRCm39)	P128S	possibly damaging	Het
Ago4	C	A	4: 126,400,977 (GRCm39)	V623L	probably benign	Het
Akap11	A	T	14: 78,753,787 (GRCm39)	N112K		Het
C1ra	A	T	6: 124,494,654 (GRCm39)	I306F	probably damaging	Het
Ccdc73	A	G	2: 104,822,222 (GRCm39)	N724D	possibly damaging	Het
Cd248	A	G	19: 5,119,645 (GRCm39)	I498V	probably benign	Het
Clrn1	A	T	3: 58,792,314 (GRCm39)	S50T	probably benign	Het
Col7a1	A	G	9: 108,796,093 (GRCm39)	K1553R	unknown	Het
Cxcr6	A	C	9: 123,640,006 (GRCm39)	T343P	probably benign	Het
Cyp11b2	T	A	15: 74,727,914 (GRCm39)	Q56L	probably damaging	Het
Dip2a	A	G	10: 76,114,320 (GRCm39)		probably null	Het
Dnmbp	A	G	19: 43,838,276 (GRCm39)	V742A	probably benign	Het
Dusp16	A	T	6: 134,716,732 (GRCm39)	S192T	probably benign	Het
Ehbp1	A	T	11: 22,182,053 (GRCm39)	D87E	probably damaging	Het
Fgl1	A	G	8: 41,652,748 (GRCm39)	V150A	probably benign	Het
Flt3	T	C	5: 147,271,673 (GRCm39)	D873G	probably damaging	Het
Gart	T	C	16: 91,427,591 (GRCm39)	D469G	possibly damaging	Het
Gm28168	T	G	1: 117,875,625 (GRCm39)	S85A	probably benign	Het
Golgb1	A	G	16: 36,736,051 (GRCm39)	D1807G	probably benign	Het
Grm8	A	G	6: 27,761,351 (GRCm39)	L291S	probably damaging	Het
Gucy2d	C	T	7: 98,092,676 (GRCm39)	P18S	possibly damaging	Het
Ifna15	C	T	4: 88,475,998 (GRCm39)	C162Y	probably damaging	Het
Iqub	C	A	6: 24,479,307 (GRCm39)	E412*	probably null	Het
Krt4	T	A	15: 101,829,077 (GRCm39)	D312V		Het
Krtap26-1	T	C	16: 88,444,324 (GRCm39)	Y99C	probably damaging	Het
Krtap26-1	A	T	16: 88,444,303 (GRCm39)	I106N	probably damaging	Het
Lipn	T	C	19: 34,062,116 (GRCm39)	I357T	probably benign	Het
Lrrc14b	A	G	13: 74,512,068 (GRCm39)	L4P	probably damaging	Het
Myh7b	A	G	2: 155,472,301 (GRCm39)	N1291D	probably benign	Het
Myo5a	A	G	9: 75,074,328 (GRCm39)	T746A	probably damaging	Het
Myom2	A	C	8: 15,164,169 (GRCm39)	E1021D	possibly damaging	Het
Ncoa2	C	T	1: 13,247,409 (GRCm39)	R338H	probably benign	Het
Ncor2	A	G	5: 125,195,821 (GRCm39)	F91L		Het
Neb	C	G	2: 52,106,923 (GRCm39)	A4407P	probably damaging	Het
Or10u4	C	T	10: 129,801,661 (GRCm39)	V297M	probably damaging	Het
Or1j18	A	T	2: 36,625,203 (GRCm39)	Y290F	probably damaging	Het
Or4b1b	A	G	2: 90,126,356 (GRCm39)	I283T	probably damaging	Het
Or5d47	A	G	2: 87,804,347 (GRCm39)	F221L	probably benign	Het
Or5p76	T	G	7: 108,122,350 (GRCm39)	Y269S	probably benign	Het
Or5w12	T	C	2: 87,502,304 (GRCm39)	M136V	possibly damaging	Het
Peg10	T	TCCC	6: 4,756,451 (GRCm39)		probably benign	Het
Piwil4	C	A	9: 14,638,771 (GRCm39)	K298N	probably benign	Het
Prkcz	G	A	4: 155,429,285 (GRCm39)		probably benign	Het
Prkg2	G	A	5: 99,090,067 (GRCm39)	P691L	possibly damaging	Het
Ptprc	T	A	1: 138,041,446 (GRCm39)	K89*	probably null	Het
Rapgef3	C	T	15: 97,664,789 (GRCm39)	A25T	probably benign	Het
Rreb1	C	T	13: 38,114,492 (GRCm39)	T617I	probably damaging	Het
Rsph1	A	C	17: 31,492,350 (GRCm39)	V72G	possibly damaging	Het
Shc2	A	G	10: 79,473,536 (GRCm39)	V50A	probably benign	Het
Slc38a9	G	A	13: 112,838,021 (GRCm39)	R262H	probably benign	Het
Slco6c1	T	A	1: 97,055,884 (GRCm39)	N6Y	possibly damaging	Het
Smarca5	A	G	8: 81,431,961 (GRCm39)	F886L	probably benign	Het
Tas1r2	A	G	4: 139,381,074 (GRCm39)		probably benign	Het
Tnrc6c	T	A	11: 117,630,680 (GRCm39)		probably benign	Het
Trim30b	T	A	7: 104,007,113 (GRCm39)		probably benign	Het
Trim55	T	C	3: 19,727,126 (GRCm39)	S398P	probably benign	Het
Ttc39c	T	A	18: 12,820,003 (GRCm39)		probably benign	Het
Ubxn10	A	G	4: 138,463,178 (GRCm39)		probably null	Het
Usf3	A	G	16: 44,035,976 (GRCm39)	N152S	probably benign	Het
Vps13b	T	A	15: 35,533,445 (GRCm39)	V839E	probably damaging	Het
Zeb1	T	A	18: 5,748,680 (GRCm39)		probably benign	Het
Zfp780b	T	C	7: 27,662,893 (GRCm39)	Y554C	probably benign	Het
Zhx2	C	T	15: 57,684,676 (GRCm39)	T15I	probably damaging	Het

Other mutations in Mrgpra9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Mrgpra9	APN	7	46,884,839 (GRCm39)	missense	possibly damaging	0.85
IGL00575:Mrgpra9	APN	7	46,885,053 (GRCm39)	missense	possibly damaging	0.76
IGL01649:Mrgpra9	APN	7	46,884,900 (GRCm39)	missense	probably benign	0.22
IGL03207:Mrgpra9	APN	7	46,885,385 (GRCm39)	missense	possibly damaging	0.68
R0388:Mrgpra9	UTSW	7	46,902,542 (GRCm39)	start codon destroyed	probably null	0.08
R0972:Mrgpra9	UTSW	7	46,885,203 (GRCm39)	missense	probably damaging	0.99
R1270:Mrgpra9	UTSW	7	46,902,531 (GRCm39)	critical splice donor site	probably null
R1381:Mrgpra9	UTSW	7	46,885,050 (GRCm39)	missense	possibly damaging	0.75
R1403:Mrgpra9	UTSW	7	46,885,386 (GRCm39)	missense	probably benign	0.24
R1403:Mrgpra9	UTSW	7	46,885,386 (GRCm39)	missense	probably benign	0.24
R1448:Mrgpra9	UTSW	7	46,885,561 (GRCm39)	missense	probably benign	0.02
R2045:Mrgpra9	UTSW	7	46,885,583 (GRCm39)	missense	probably benign
R2144:Mrgpra9	UTSW	7	46,885,211 (GRCm39)	missense	probably benign	0.31
R2187:Mrgpra9	UTSW	7	46,884,797 (GRCm39)	missense	probably damaging	1.00
R2507:Mrgpra9	UTSW	7	46,885,242 (GRCm39)	missense	possibly damaging	0.63
R2913:Mrgpra9	UTSW	7	46,884,828 (GRCm39)	missense	probably benign
R3810:Mrgpra9	UTSW	7	46,885,527 (GRCm39)	missense	probably damaging	0.98
R4177:Mrgpra9	UTSW	7	46,885,302 (GRCm39)	missense	probably damaging	1.00
R4521:Mrgpra9	UTSW	7	46,884,938 (GRCm39)	missense	probably damaging	1.00
R4781:Mrgpra9	UTSW	7	46,884,795 (GRCm39)	missense	possibly damaging	0.88
R4926:Mrgpra9	UTSW	7	46,884,759 (GRCm39)	missense	possibly damaging	0.62
R6469:Mrgpra9	UTSW	7	46,884,854 (GRCm39)	missense	probably benign	0.02
R6505:Mrgpra9	UTSW	7	46,884,884 (GRCm39)	missense	probably benign	0.00
R6724:Mrgpra9	UTSW	7	46,884,786 (GRCm39)	missense	probably damaging	1.00
R7398:Mrgpra9	UTSW	7	46,885,385 (GRCm39)	missense	possibly damaging	0.68
R8737:Mrgpra9	UTSW	7	46,885,624 (GRCm39)	missense	probably benign	0.00
R8881:Mrgpra9	UTSW	7	46,885,242 (GRCm39)	missense	possibly damaging	0.63
R9320:Mrgpra9	UTSW	7	46,885,392 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CAACCGATGCCTGAATGAGC -3'
(R):5'- AGAACACACATCAACTGTCATGTG -3'

Sequencing Primer
(F):5'- TAGCACTCAGGACAGTTGATGC -3'
(R):5'- ACACATCAACTGTCATGTGTGCTG -3'

Posted On

2021-07-15