Mutagenetix > Incidental Mutation

Incidental Mutation 'R8825:Or8g4'

673393

Institutional Source

Beutler Lab

Gene Symbol

Or8g4

Ensembl Gene

ENSMUSG00000055820

Gene Name

olfactory receptor family 8 subfamily G member 4

Synonyms

Olfr967, GA_x6K02T2PVTD-33447884-33448816, MOR171-30P

MMRRC Submission

068728-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.084) question?

Stock #

R8825 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

39661684-39662616 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 39661994 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 104 (F104S)

Ref Sequence

ENSEMBL: ENSMUSP00000150183 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069561] [ENSMUST00000213358]

AlphaFold

Q7TRA6

Predicted Effect

probably damaging
Transcript: ENSMUST00000069561
AA Change: F104S

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000064201
Gene: ENSMUSG00000055820
AA Change: F104S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	5.6e-50	PFAM
Pfam:7tm_1	41	290	6.8e-22	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000213358
AA Change: F104S

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

100% (61/61)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arfgap2	T	A	2: 91,103,906 (GRCm39)	L334Q	probably damaging	Het
Arhgef18	T	A	8: 3,436,951 (GRCm39)	L247Q	probably damaging	Het
Birc6	T	A	17: 74,920,500 (GRCm39)	C2100S	probably damaging	Het
Bpi	A	G	2: 158,109,670 (GRCm39)	D206G	probably benign	Het
C6	T	C	15: 4,761,170 (GRCm39)	I10T	possibly damaging	Het
Cactin	A	G	10: 81,161,492 (GRCm39)	T675A	probably damaging	Het
Cd200	G	A	16: 45,215,157 (GRCm39)	R165C	probably benign	Het
Cdkal1	C	T	13: 29,538,777 (GRCm39)	V461I	probably benign	Het
Ces2g	C	T	8: 105,693,954 (GRCm39)	S430F	probably benign	Het
Clcn3	A	T	8: 61,382,522 (GRCm39)	H382Q	probably damaging	Het
Crem	A	T	18: 3,268,061 (GRCm39)	V89E	probably damaging	Het
Crtc2	T	A	3: 90,166,463 (GRCm39)	M146K	probably benign	Het
Depdc1b	T	G	13: 108,521,316 (GRCm39)	D374E	possibly damaging	Het
Dgkg	T	A	16: 22,381,519 (GRCm39)	H477L	probably benign	Het
Dnhd1	T	A	7: 105,343,174 (GRCm39)	V1506D	possibly damaging	Het
Erfl	A	G	7: 24,628,682 (GRCm39)	V60A	possibly damaging	Het
Exosc10	G	A	4: 148,653,159 (GRCm39)		probably null	Het
Fem1al	T	C	11: 29,773,696 (GRCm39)	E587G	probably benign	Het
Flnb	G	T	14: 7,887,566 (GRCm38)	G459C	probably damaging	Het
Golgb1	A	G	16: 36,739,809 (GRCm39)	D2757G	probably benign	Het
Gsta4	T	G	9: 78,116,121 (GRCm39)		probably benign	Het
Herc2	T	A	7: 55,700,626 (GRCm39)	M1K	probably null	Het
Hip1	C	T	5: 135,450,976 (GRCm39)	V879M	probably damaging	Het
Htt	T	C	5: 34,983,304 (GRCm39)	Y968H	probably benign	Het
Ints11	T	A	4: 155,969,587 (GRCm39)	Y154*	probably null	Het
Itih5	A	G	2: 10,195,231 (GRCm39)	S208G	probably benign	Het
Lamb2	C	T	9: 108,362,460 (GRCm39)	T701I	probably benign	Het
Lgals3	T	A	14: 47,617,557 (GRCm39)	Y22*	probably null	Het
Macc1	T	A	12: 119,409,587 (GRCm39)	D118E	probably benign	Het
Mad2l2	T	C	4: 148,225,277 (GRCm39)	L9P	probably damaging	Het
Mark2	T	C	19: 7,318,571 (GRCm39)	T6A	probably benign	Het
Mcat	T	C	15: 83,436,812 (GRCm39)	N143S	probably benign	Het
Med10	T	G	13: 69,962,046 (GRCm39)	C144G	unknown	Het
Miga1	A	T	3: 151,982,460 (GRCm39)	F539I	probably damaging	Het
Mon2	A	T	10: 122,849,776 (GRCm39)	S1174T	probably benign	Het
Mycbp2	C	A	14: 103,466,871 (GRCm39)	W1330C	probably damaging	Het
Myo5b	T	C	18: 74,892,169 (GRCm39)	S1612P	possibly damaging	Het
Nipal4	T	C	11: 46,052,873 (GRCm39)	I31V	probably benign	Het
Obox3	A	T	7: 15,361,226 (GRCm39)	V13E	possibly damaging	Het
Or2ag19	A	T	7: 106,444,636 (GRCm39)	I273F	probably damaging	Het
Ppic	A	G	18: 53,542,222 (GRCm39)	V162A	probably damaging	Het
Prkg2	T	C	5: 99,090,043 (GRCm39)	K699R	probably benign	Het
Prl2c2	T	A	13: 13,179,656 (GRCm39)	L6F	possibly damaging	Het
Rnf148	G	C	6: 23,654,378 (GRCm39)	S206C	probably benign	Het
Sec16a	A	G	2: 26,313,586 (GRCm39)	S474P		Het
Sema5a	T	G	15: 32,689,498 (GRCm39)	H1054Q	probably benign	Het
Slc6a18	C	A	13: 73,813,751 (GRCm39)	V521F	probably null	Het
Stat2	T	A	10: 128,127,233 (GRCm39)	D921E	possibly damaging	Het
Tcf24	G	T	1: 10,031,224 (GRCm39)	N42K	unknown	Het
Tgfb2	T	A	1: 186,361,136 (GRCm39)	N372Y	probably damaging	Het
Tktl2	A	C	8: 66,966,319 (GRCm39)	M626L	possibly damaging	Het
Tmem94	A	G	11: 115,688,201 (GRCm39)	D1220G	probably benign	Het
Trcg1	A	G	9: 57,148,754 (GRCm39)	T109A	probably benign	Het
Trim30a	T	A	7: 104,060,529 (GRCm39)	K416*	probably null	Het
Trpm5	T	C	7: 142,636,753 (GRCm39)	K395E	possibly damaging	Het
Ufm1	T	A	3: 53,771,093 (GRCm39)		probably null	Het
Unc13b	T	A	4: 43,237,683 (GRCm39)		probably benign	Het
Vmn2r16	T	C	5: 109,487,019 (GRCm39)	V80A	probably benign	Het
Vmn2r77	A	T	7: 86,452,855 (GRCm39)	Y524F	probably benign	Het
Zfp1007	T	G	5: 109,826,746 (GRCm39)	T5P	probably damaging	Het
Zfp64	T	C	2: 168,793,552 (GRCm39)	S65G	probably benign	Het
Zng1	A	G	19: 24,926,601 (GRCm39)	V150A	probably benign	Het

Other mutations in Or8g4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02418:Or8g4	APN	9	39,661,787 (GRCm39)	missense	probably damaging	0.99
IGL02900:Or8g4	APN	9	39,661,901 (GRCm39)	missense	probably benign	0.01
R0099:Or8g4	UTSW	9	39,661,957 (GRCm39)	missense	possibly damaging	0.95
R0586:Or8g4	UTSW	9	39,662,414 (GRCm39)	missense	probably damaging	0.98
R0653:Or8g4	UTSW	9	39,661,934 (GRCm39)	missense	probably benign	0.26
R0839:Or8g4	UTSW	9	39,661,687 (GRCm39)	missense	probably benign
R1701:Or8g4	UTSW	9	39,662,365 (GRCm39)	missense	probably damaging	1.00
R1744:Or8g4	UTSW	9	39,661,711 (GRCm39)	missense	probably benign	0.33
R1902:Or8g4	UTSW	9	39,662,102 (GRCm39)	missense	probably benign	0.01
R4696:Or8g4	UTSW	9	39,662,024 (GRCm39)	missense	probably damaging	0.98
R5252:Or8g4	UTSW	9	39,661,784 (GRCm39)	missense	probably damaging	0.98
R5660:Or8g4	UTSW	9	39,662,063 (GRCm39)	missense	probably damaging	1.00
R6272:Or8g4	UTSW	9	39,661,816 (GRCm39)	missense	probably benign	0.39
R6976:Or8g4	UTSW	9	39,662,540 (GRCm39)	missense	probably damaging	1.00
R7078:Or8g4	UTSW	9	39,661,787 (GRCm39)	missense	possibly damaging	0.92
R7167:Or8g4	UTSW	9	39,661,865 (GRCm39)	missense	probably damaging	0.96
R7701:Or8g4	UTSW	9	39,662,597 (GRCm39)	missense	probably benign
R7980:Or8g4	UTSW	9	39,662,417 (GRCm39)	missense	probably damaging	0.99
R8026:Or8g4	UTSW	9	39,662,092 (GRCm39)	missense	possibly damaging	0.80
R8701:Or8g4	UTSW	9	39,662,210 (GRCm39)	missense	probably damaging	1.00
R8790:Or8g4	UTSW	9	39,662,204 (GRCm39)	missense	probably damaging	1.00
R8822:Or8g4	UTSW	9	39,661,700 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TCTATGCAGTAACGATGGTAGG -3'
(R):5'- TGGACTCTTAGCATGCACACTG -3'

Sequencing Primer
(F):5'- GGGCATGATCACACTGATTCTGC -3'
(R):5'- CACTGTGTGAGCTGTGGCAC -3'

Posted On

2021-07-15