Mutagenetix > Incidental Mutation

Incidental Mutation 'R8828:Sema4f'

673605

Institutional Source

Beutler Lab

Gene Symbol

Sema4f

Ensembl Gene

ENSMUSG00000000627

Gene Name

sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain

Synonyms

Sema W

MMRRC Submission

068730-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8828 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

82888865-82916724 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 82894873 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 441 (L441F)

Ref Sequence

ENSEMBL: ENSMUSP00000000641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000000641] [ENSMUST00000203271]

AlphaFold

Q9Z123

Predicted Effect

probably benign
Transcript: ENSMUST00000000641
AA Change: L441F

PolyPhen 2 Score 0.018 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000000641
Gene: ENSMUSG00000000627
AA Change: L441F

Domain	Start	End	E-Value	Type
signal peptide	1	40	N/A	INTRINSIC
Sema	71	502	2.23e-170	SMART
PSI	518	569	2.64e-12	SMART
Blast:Sema	607	656	5e-20	BLAST
transmembrane domain	665	687	N/A	INTRINSIC
low complexity region	722	735	N/A	INTRINSIC
low complexity region	743	751	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000203271

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a member of semaphorin family of membrane-bound and secreted proteins that are involved in guiding axonal growth. The encoded protein is a transmembrane protein localized to the glutamatergic synapses via its association with a synapse-associated scaffolding protein. In oligodendrocyte precursor cells, the encoded protein contributes to the outward migration and differentiation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2015]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abi3bp	A	T	16: 56,507,455 (GRCm39)	Y1091F	probably damaging	Het
Arl8b	A	G	6: 108,792,250 (GRCm39)	I104V	probably benign	Het
Asb2	C	A	12: 103,304,457 (GRCm39)	G16C	probably benign	Het
Bptf	T	C	11: 106,945,836 (GRCm39)	S2468G	probably damaging	Het
Capns1	T	A	7: 29,889,963 (GRCm39)	D182V	probably damaging	Het
Cct5	T	C	15: 31,594,658 (GRCm39)	H182R	possibly damaging	Het
Cd200	G	A	16: 45,215,157 (GRCm39)	R165C	probably benign	Het
Chd3	C	T	11: 69,247,097 (GRCm39)	V1024I	probably damaging	Het
Chd8	CAGGGTCTGCCCTCATGGTATTGTATTTCTCATAGCCTAAAAGAACAAGGG	C	14: 52,448,037 (GRCm39)		probably null	Het
Csmd1	T	C	8: 16,048,794 (GRCm39)	S2303G	probably benign	Het
Ctsl	A	G	13: 64,514,314 (GRCm39)	S255P	probably damaging	Het
Dclre1c	T	G	2: 3,444,714 (GRCm39)	N268K	possibly damaging	Het
Dock10	T	C	1: 80,521,134 (GRCm39)	D1158G	probably damaging	Het
Dock6	A	T	9: 21,757,797 (GRCm39)	D102E	probably benign	Het
Dvl3	T	A	16: 20,344,495 (GRCm39)	D290E	probably damaging	Het
Fam72a	T	A	1: 131,458,461 (GRCm39)	C74*	probably null	Het
Gnrh1	T	A	14: 67,985,334 (GRCm39)	L73Q	probably damaging	Het
Grina	T	A	15: 76,132,497 (GRCm39)	S115T	probably damaging	Het
Grm3	A	T	5: 9,554,725 (GRCm39)	S856T	probably benign	Het
Hars1	C	A	18: 36,899,996 (GRCm39)	R490L	possibly damaging	Het
Ide	T	A	19: 37,292,241 (GRCm39)	K318I		Het
Immt	C	A	6: 71,829,762 (GRCm39)	S156*	probably null	Het
Iqcd	A	G	5: 120,738,232 (GRCm39)	I17V	possibly damaging	Het
Klhdc1	T	C	12: 69,298,808 (GRCm39)	V131A	probably damaging	Het
Klk6	C	A	7: 43,478,061 (GRCm39)	N144K		Het
Klk6	T	C	7: 43,478,062 (GRCm39)	C145R	probably damaging	Het
Lrfn2	A	T	17: 49,404,132 (GRCm39)	T752S	probably damaging	Het
Lrguk	A	C	6: 34,080,572 (GRCm39)	Q1269P	unknown	Het
Lrwd1	G	T	5: 136,164,787 (GRCm39)	Q7K	probably benign	Het
Macf1	A	T	4: 123,302,204 (GRCm39)	M766K	probably benign	Het
Myo3a	A	T	2: 22,245,864 (GRCm39)	T4S	probably benign	Het
Neb	T	C	2: 52,084,438 (GRCm39)	Y5587C	probably damaging	Het
Nf1	A	T	11: 79,286,679 (GRCm39)		probably null	Het
Npepps	G	T	11: 97,131,823 (GRCm39)	R344S	probably damaging	Het
Nudt16l2	C	T	9: 105,021,648 (GRCm39)	D133N	probably damaging	Het
Or52p1	A	T	7: 104,267,486 (GRCm39)	Y200F	probably damaging	Het
Or5v1b	A	G	17: 37,841,333 (GRCm39)	N155S	probably damaging	Het
Pcnx1	T	A	12: 82,042,597 (GRCm39)	W2286R	probably damaging	Het
Ppm1g	A	T	5: 31,365,768 (GRCm39)	N51K	probably benign	Het
Ppp1r13b	T	C	12: 111,799,981 (GRCm39)	K722E	probably damaging	Het
Prim1	A	G	10: 127,859,761 (GRCm39)	S318G	probably damaging	Het
Prl4a1	T	C	13: 28,207,307 (GRCm39)	Y194H	probably damaging	Het
Prl7c1	A	T	13: 27,957,854 (GRCm39)	D195E	probably benign	Het
Psmb2	A	G	4: 126,603,330 (GRCm39)	D186G	probably benign	Het
Ptprq	A	G	10: 107,482,513 (GRCm39)	V1020A	probably benign	Het
Scgb2b20	T	C	7: 33,065,689 (GRCm39)	T13A	unknown	Het
Scly	C	A	1: 91,244,830 (GRCm39)	H310Q	possibly damaging	Het
Sdccag8	G	A	1: 176,783,473 (GRCm39)	E661K	probably damaging	Het
Tmem262	C	T	19: 6,130,118 (GRCm39)		probably benign	Het
Trim33	A	G	3: 103,236,392 (GRCm39)	T501A	probably damaging	Het
Trio	C	A	15: 27,741,150 (GRCm39)	V2692F	possibly damaging	Het
Ttn	A	T	2: 76,772,774 (GRCm39)	V2453E	unknown	Het
Ttn	G	T	2: 76,541,956 (GRCm39)	R33677S	probably benign	Het
Uaca	T	A	9: 60,778,852 (GRCm39)	S1080T	probably benign	Het
Vav3	T	A	3: 109,555,051 (GRCm39)	C660S	probably benign	Het
Vmn1r229	T	A	17: 21,034,990 (GRCm39)	D78E	probably damaging	Het
Vmn2r73	A	G	7: 85,521,179 (GRCm39)	V263A	probably damaging	Het
Yeats2	A	T	16: 19,969,260 (GRCm39)	D17V	probably benign	Het
Zfp658	T	A	7: 43,222,240 (GRCm39)	W172R	probably benign	Het
Zfp804a	A	C	2: 82,089,459 (GRCm39)	H1096P	possibly damaging	Het
Zkscan14	C	T	5: 145,138,375 (GRCm39)	W37*	probably null	Het
Zmynd15	A	T	11: 70,355,017 (GRCm39)	D468V	probably damaging	Het
Zmynd8	A	T	2: 165,654,466 (GRCm39)	V682E	probably benign	Het

Other mutations in Sema4f

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00339:Sema4f	APN	6	82,914,155 (GRCm39)	missense	probably benign	0.00
IGL01661:Sema4f	APN	6	82,895,036 (GRCm39)	unclassified	probably benign
docking	UTSW	6	82,890,626 (GRCm39)	nonsense	probably null
flagman	UTSW	6	82,895,540 (GRCm39)	missense	probably benign	0.02
R0054:Sema4f	UTSW	6	82,896,674 (GRCm39)	splice site	probably benign
R0054:Sema4f	UTSW	6	82,896,674 (GRCm39)	splice site	probably benign
R0243:Sema4f	UTSW	6	82,916,447 (GRCm39)	missense	possibly damaging	0.87
R0692:Sema4f	UTSW	6	82,916,511 (GRCm39)	unclassified	probably benign
R0893:Sema4f	UTSW	6	82,912,948 (GRCm39)	splice site	probably benign
R1708:Sema4f	UTSW	6	82,894,975 (GRCm39)	missense	probably damaging	1.00
R1833:Sema4f	UTSW	6	82,895,540 (GRCm39)	missense	probably benign	0.02
R1867:Sema4f	UTSW	6	82,894,824 (GRCm39)	missense	possibly damaging	0.84
R1899:Sema4f	UTSW	6	82,895,010 (GRCm39)	missense	probably benign	0.00
R1933:Sema4f	UTSW	6	82,907,908 (GRCm39)	missense	probably damaging	1.00
R1934:Sema4f	UTSW	6	82,907,908 (GRCm39)	missense	probably damaging	1.00
R2433:Sema4f	UTSW	6	82,916,490 (GRCm39)	missense	possibly damaging	0.66
R3801:Sema4f	UTSW	6	82,895,608 (GRCm39)	missense	possibly damaging	0.88
R4116:Sema4f	UTSW	6	82,894,887 (GRCm39)	missense	probably benign	0.25
R4745:Sema4f	UTSW	6	82,895,265 (GRCm39)	missense	probably damaging	1.00
R5187:Sema4f	UTSW	6	82,894,631 (GRCm39)	missense	probably benign	0.45
R6015:Sema4f	UTSW	6	82,916,553 (GRCm39)	unclassified	probably benign
R6043:Sema4f	UTSW	6	82,896,634 (GRCm39)	missense	probably damaging	0.99
R6110:Sema4f	UTSW	6	82,914,085 (GRCm39)	missense	probably damaging	0.97
R6378:Sema4f	UTSW	6	82,894,613 (GRCm39)	nonsense	probably null
R6449:Sema4f	UTSW	6	82,894,851 (GRCm39)	missense	probably benign	0.09
R6452:Sema4f	UTSW	6	82,894,643 (GRCm39)	missense	probably benign	0.36
R6854:Sema4f	UTSW	6	82,894,983 (GRCm39)	missense	probably damaging	1.00
R7159:Sema4f	UTSW	6	82,894,864 (GRCm39)	missense	possibly damaging	0.63
R7475:Sema4f	UTSW	6	82,891,355 (GRCm39)	missense	possibly damaging	0.94
R7555:Sema4f	UTSW	6	82,891,037 (GRCm39)	missense	probably benign	0.01
R7780:Sema4f	UTSW	6	82,890,941 (GRCm39)	missense	possibly damaging	0.95
R8254:Sema4f	UTSW	6	82,894,382 (GRCm39)	missense	probably damaging	1.00
R8828:Sema4f	UTSW	6	82,894,874 (GRCm39)	nonsense	probably null
R8862:Sema4f	UTSW	6	82,891,081 (GRCm39)	missense	probably benign	0.02
R9166:Sema4f	UTSW	6	82,890,626 (GRCm39)	nonsense	probably null
R9218:Sema4f	UTSW	6	82,890,480 (GRCm39)	missense	probably benign	0.08
R9340:Sema4f	UTSW	6	82,890,890 (GRCm39)	missense	probably damaging	1.00
R9690:Sema4f	UTSW	6	82,912,652 (GRCm39)	missense	probably damaging	1.00
X0026:Sema4f	UTSW	6	82,912,661 (GRCm39)	missense	probably benign	0.10

Predicted Primers

PCR Primer
(F):5'- ATGGCTTAGCTGGCACTGTG -3'
(R):5'- CGTAAAGGACAAGAGCTTTGC -3'

Sequencing Primer
(F):5'- ACTGTGCCTGGAACCCTG -3'
(R):5'- GTTCCCTGAGAAACATTGTCAC -3'

Posted On

2021-07-15