Mutagenetix > Incidental Mutation

Incidental Mutation 'R8829:Or10j7'

673651

Institutional Source

Beutler Lab

Gene Symbol

Or10j7

Ensembl Gene

ENSMUSG00000058981

Gene Name

olfactory receptor family 10 subfamily J member 7

Synonyms

GA_x6K02T2R7CC-664297-665229, MOR267-5, Olfr1406

MMRRC Submission

068731-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R8829 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

173011009-173012071 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 173011458 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 181 (R181H)

Ref Sequence

ENSEMBL: ENSMUSP00000144530 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072395] [ENSMUST00000201132] [ENSMUST00000215878]

AlphaFold

E9Q8X1

Predicted Effect

probably benign
Transcript: ENSMUST00000072395
AA Change: R181H

PolyPhen 2 Score 0.058 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000072231
Gene: ENSMUSG00000058981
AA Change: R181H

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	3.9e-55	PFAM
Pfam:7tm_1	41	289	2.7e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000201132
AA Change: R181H

PolyPhen 2 Score 0.058 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000144530
Gene: ENSMUSG00000058981
AA Change: R181H

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	88	5.4e-11	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215878
AA Change: R181H

PolyPhen 2 Score 0.058 (Sensitivity: 0.94; Specificity: 0.84)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadac	A	T	3: 59,939,240 (GRCm39)	I5F	probably benign	Het
Abca13	A	T	11: 9,571,881 (GRCm39)	D4814V	probably damaging	Het
Adgrl1	A	G	8: 84,665,458 (GRCm39)	N1382D		Het
Adh6b	T	C	3: 138,055,463 (GRCm39)	V71A	probably benign	Het
Aox4	A	T	1: 58,294,649 (GRCm39)	M953L	probably benign	Het
B3galt9	T	A	2: 34,728,634 (GRCm39)	N144K	probably benign	Het
Bach2	A	T	4: 32,562,028 (GRCm39)	E165V	probably damaging	Het
Bltp2	A	G	11: 78,158,064 (GRCm39)	K409E	probably benign	Het
Catsperg2	A	G	7: 29,397,269 (GRCm39)	V1078A	probably benign	Het
Ccno	A	G	13: 113,126,239 (GRCm39)	N236S	probably benign	Het
Ccpg1	A	T	9: 72,917,633 (GRCm39)	D255V	probably benign	Het
Cdhr2	A	T	13: 54,865,930 (GRCm39)	Y278F	probably damaging	Het
Cdk17	C	T	10: 93,042,920 (GRCm39)		probably benign	Het
Celsr3	A	G	9: 108,717,582 (GRCm39)	D2216G	probably benign	Het
Cenpu	T	C	8: 47,026,496 (GRCm39)	L294P	probably damaging	Het
Chct1	A	G	11: 85,062,037 (GRCm39)	D12G	probably damaging	Het
Ctif	CGGGGCACACTTTGCTCTTACCTCCCGGAGGCACGTGTAGATGGGGCACACTTTGCTCTTACCTCCCGGAGGCACGTGTAGATGGGGCACAC	CGGGGCACACTTTGCTCTTACCTCCCGGAGGCACGTGTAGATGGGGCACAC	18: 75,604,874 (GRCm39)		probably benign	Het
Ctnna2	G	A	6: 77,582,205 (GRCm39)	R352*	probably null	Het
D630003M21Rik	C	A	2: 158,058,856 (GRCm39)	C348F	probably damaging	Het
Dcdc2a	C	T	13: 25,294,051 (GRCm39)	Q236*	probably null	Het
Ddx60	T	G	8: 62,393,695 (GRCm39)	S44A	probably damaging	Het
Dennd2c	T	A	3: 103,059,720 (GRCm39)		probably null	Het
Drd5	G	A	5: 38,477,078 (GRCm39)	V24M	probably benign	Het
Dthd1	T	C	5: 62,971,608 (GRCm39)	S144P	probably benign	Het
Fcna	C	A	2: 25,516,145 (GRCm39)	R124L	possibly damaging	Het
Frem1	T	C	4: 82,918,431 (GRCm39)	R504G	probably damaging	Het
Gldc	C	G	19: 30,078,212 (GRCm39)	M928I	probably benign	Het
Gprc6a	A	C	10: 51,491,295 (GRCm39)	I818R	probably damaging	Het
Hcfc2	T	C	10: 82,574,179 (GRCm39)	Y118H	probably damaging	Het
Hmmr	G	A	11: 40,612,499 (GRCm39)	S206F	probably damaging	Het
Htr1d	A	T	4: 136,170,554 (GRCm39)	H261L	probably benign	Het
Hycc2	A	T	1: 58,587,832 (GRCm39)	I127N	possibly damaging	Het
Igf1r	T	A	7: 67,875,769 (GRCm39)	F1244I	probably damaging	Het
Ighv1-63	A	G	12: 115,459,534 (GRCm39)	V21A	probably benign	Het
Kit	A	G	5: 75,799,791 (GRCm39)	N508D	probably benign	Het
Klk1b22	A	T	7: 43,764,277 (GRCm39)	E68D	probably benign	Het
Knstrn	C	T	2: 118,654,222 (GRCm39)	Q211*	probably null	Het
Krtap2-4	A	T	11: 99,505,246 (GRCm39)	C122S	unknown	Het
Map3k1	T	G	13: 111,889,015 (GRCm39)	H1314P	possibly damaging	Het
Mgat4c	A	T	10: 102,214,084 (GRCm39)	K22N	probably damaging	Het
Mpo	T	A	11: 87,694,250 (GRCm39)	F660Y	probably damaging	Het
Myb	A	T	10: 21,021,130 (GRCm39)	L433H	probably damaging	Het
Myo1c	G	A	11: 75,561,072 (GRCm39)	V793I	probably benign	Het
Nckap1l	T	A	15: 103,387,242 (GRCm39)	S706T	probably benign	Het
Ncoa3	A	G	2: 165,892,068 (GRCm39)	Y148C	probably damaging	Het
Nktr	T	A	9: 121,583,330 (GRCm39)	S1432T	unknown	Het
Odf2l	C	T	3: 144,833,820 (GRCm39)	S160L	probably benign	Het
Or56b34	T	C	7: 104,937,435 (GRCm39)	L45P	possibly damaging	Het
Or6x1	T	A	9: 40,099,209 (GRCm39)	F266Y	probably benign	Het
Or8d23	G	T	9: 38,842,190 (GRCm39)	C241F	probably damaging	Het
Pclo	T	C	5: 14,838,464 (GRCm39)	W1424R		Het
Pcx	A	G	19: 4,651,968 (GRCm39)	D72G	probably damaging	Het
Pebp4	A	G	14: 70,285,916 (GRCm39)	D193G	probably benign	Het
Pi4kb	C	T	3: 94,900,344 (GRCm39)	T326M	probably damaging	Het
Piezo1	A	C	8: 123,217,753 (GRCm39)	M1265R		Het
Pip4k2c	A	T	10: 127,037,037 (GRCm39)	H177Q	probably damaging	Het
Pramel48	G	T	5: 95,630,939 (GRCm39)	C272F	possibly damaging	Het
Prpf40a	T	C	2: 53,047,927 (GRCm39)	M197V	probably benign	Het
Ptger3	A	G	3: 157,273,423 (GRCm39)	T257A	probably damaging	Het
Relt	T	A	7: 100,499,479 (GRCm39)	S147C	probably damaging	Het
Scap	A	G	9: 110,209,271 (GRCm39)	Y648C	probably damaging	Het
Scn9a	T	A	2: 66,313,961 (GRCm39)	D1919V	probably benign	Het
Septin8	G	A	11: 53,422,865 (GRCm39)	V25I	probably damaging	Het
Serpinb2	A	G	1: 107,443,257 (GRCm39)	I19V	probably benign	Het
Slc16a6	A	G	11: 109,345,932 (GRCm39)	Y444H	probably benign	Het
Syne1	T	C	10: 5,058,685 (GRCm39)	E7276G	probably benign	Het
Syt1	C	A	10: 108,478,193 (GRCm39)	C77F	probably benign	Het
Tcf20	T	A	15: 82,739,915 (GRCm39)	K512M	probably damaging	Het
Tomm7	T	C	5: 24,049,047 (GRCm39)	K9E	possibly damaging	Het
Trank1	T	C	9: 111,176,591 (GRCm39)	S288P	probably benign	Het
Trem3	T	C	17: 48,556,865 (GRCm39)	V112A	probably benign	Het
Ttbk1	C	A	17: 46,757,821 (GRCm39)	G938W	probably damaging	Het
Vmn1r178	G	A	7: 23,593,264 (GRCm39)	C104Y	probably damaging	Het
Zpr1	T	A	9: 46,192,344 (GRCm39)	Y418*	probably null	Het

Other mutations in Or10j7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00946:Or10j7	APN	1	173,011,190 (GRCm39)	missense	probably benign	0.45
IGL01609:Or10j7	APN	1	173,011,843 (GRCm39)	missense	probably benign	0.00
IGL01996:Or10j7	APN	1	173,011,294 (GRCm39)	missense	probably benign	0.00
F5770:Or10j7	UTSW	1	173,011,531 (GRCm39)	missense	probably benign	0.05
PIT4378001:Or10j7	UTSW	1	173,011,381 (GRCm39)	missense	probably benign	0.00
R0053:Or10j7	UTSW	1	173,011,845 (GRCm39)	missense	probably benign	0.35
R0800:Or10j7	UTSW	1	173,011,627 (GRCm39)	missense	probably damaging	1.00
R1793:Or10j7	UTSW	1	173,011,976 (GRCm39)	missense	probably benign
R2230:Or10j7	UTSW	1	173,011,182 (GRCm39)	missense	probably benign	0.04
R2232:Or10j7	UTSW	1	173,011,182 (GRCm39)	missense	probably benign	0.04
R5395:Or10j7	UTSW	1	173,011,247 (GRCm39)	nonsense	probably null
R5455:Or10j7	UTSW	1	173,011,818 (GRCm39)	missense	probably damaging	1.00
R5457:Or10j7	UTSW	1	173,011,180 (GRCm39)	missense	probably damaging	1.00
R5558:Or10j7	UTSW	1	173,011,585 (GRCm39)	missense	probably benign	0.01
R5760:Or10j7	UTSW	1	173,011,318 (GRCm39)	missense	probably benign	0.05
R6285:Or10j7	UTSW	1	173,011,477 (GRCm39)	missense	probably damaging	1.00
R7159:Or10j7	UTSW	1	173,011,890 (GRCm39)	missense	possibly damaging	0.95
R7676:Or10j7	UTSW	1	173,011,120 (GRCm39)	nonsense	probably null
R8700:Or10j7	UTSW	1	173,011,429 (GRCm39)	missense	probably benign
R9721:Or10j7	UTSW	1	173,011,915 (GRCm39)	missense	probably benign	0.07
R9785:Or10j7	UTSW	1	173,011,458 (GRCm39)	missense	probably benign	0.06
R9788:Or10j7	UTSW	1	173,011,458 (GRCm39)	missense	probably benign	0.06
R9789:Or10j7	UTSW	1	173,011,458 (GRCm39)	missense	probably benign	0.06
V7580:Or10j7	UTSW	1	173,011,531 (GRCm39)	missense	probably benign	0.05
V7581:Or10j7	UTSW	1	173,011,531 (GRCm39)	missense	probably benign	0.05
V7582:Or10j7	UTSW	1	173,011,531 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- GCACAGCCATAGTGGATCAC -3'
(R):5'- ATCGCTATGTGGCCATCTGC -3'

Sequencing Primer
(F):5'- TCACAACCACAGTGAGGTG -3'
(R):5'- GCAACCCGCTTCGCTATAG -3'

Posted On

2021-07-15