Mutagenetix > Incidental Mutation

Incidental Mutation 'R8850:Tnn'

674916

Institutional Source

Beutler Lab

Gene Symbol

Tnn

Ensembl Gene

ENSMUSG00000026725

Gene Name

tenascin N

Synonyms

tenascin-W, Tnw

MMRRC Submission

068673-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.396) question?

Stock #

R8850 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

159912599-159981150 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to G at 159937814 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000039452 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039178] [ENSMUST00000039178] [ENSMUST00000131919]

AlphaFold

Q80Z71

Predicted Effect

probably null
Transcript: ENSMUST00000039178

SMART Domains

Protein: ENSMUSP00000039452
Gene: ENSMUSG00000026725

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
coiled coil region	100	132	N/A	INTRINSIC
EGF_like	170	198	3.5e1	SMART
EGF	201	229	2.29e1	SMART
EGF_like	232	260	2.86e1	SMART
FN3	262	341	1.81e-8	SMART
FN3	351	432	1.08e-6	SMART
FN3	443	521	1.19e-8	SMART
FN3	531	608	2.64e-10	SMART
FN3	619	696	1.6e-9	SMART
FN3	707	784	9.04e-9	SMART
FN3	795	872	7.34e-9	SMART
FN3	883	960	9.04e-9	SMART
FN3	971	1048	1.07e-10	SMART
FN3	1059	1136	7.57e-11	SMART
FN3	1147	1224	4.59e-10	SMART
FN3	1235	1312	1.95e-4	SMART
FBG	1327	1539	1.16e-114	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000039178

SMART Domains

Protein: ENSMUSP00000039452
Gene: ENSMUSG00000026725

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
coiled coil region	100	132	N/A	INTRINSIC
EGF_like	170	198	3.5e1	SMART
EGF	201	229	2.29e1	SMART
EGF_like	232	260	2.86e1	SMART
FN3	262	341	1.81e-8	SMART
FN3	351	432	1.08e-6	SMART
FN3	443	521	1.19e-8	SMART
FN3	531	608	2.64e-10	SMART
FN3	619	696	1.6e-9	SMART
FN3	707	784	9.04e-9	SMART
FN3	795	872	7.34e-9	SMART
FN3	883	960	9.04e-9	SMART
FN3	971	1048	1.07e-10	SMART
FN3	1059	1136	7.57e-11	SMART
FN3	1147	1224	4.59e-10	SMART
FN3	1235	1312	1.95e-4	SMART
FBG	1327	1539	1.16e-114	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131919

SMART Domains

Protein: ENSMUSP00000115685
Gene: ENSMUSG00000026725

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
coiled coil region	100	132	N/A	INTRINSIC
EGF_like	170	198	3.5e1	SMART
EGF	201	229	2.29e1	SMART
EGF_like	232	260	2.86e1	SMART
FN3	262	341	1.81e-8	SMART
FN3	351	432	1.08e-6	SMART
FN3	443	521	1.19e-8	SMART
FN3	531	608	2.64e-10	SMART
FN3	619	696	1.6e-9	SMART
FN3	707	784	9.04e-9	SMART
FN3	795	872	7.57e-11	SMART
FN3	883	960	4.59e-10	SMART
FN3	971	1048	1.95e-4	SMART
FBG	1063	1275	1.16e-114	SMART

Meta Mutation Damage Score

0.9491

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (64/64)

Allele List at MGI

All alleles(2) : Targeted, other(2)

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actr10	G	A	12: 70,989,032 (GRCm39)		probably null	Het
Adgrd1	A	G	5: 129,219,574 (GRCm39)	K465E	probably benign	Het
Aktip	A	T	8: 91,853,402 (GRCm39)	V105E	probably benign	Het
Ankrd7	A	G	6: 18,870,006 (GRCm39)	Y253C	probably damaging	Het
Bag6	T	C	17: 35,361,041 (GRCm39)	M440T	unknown	Het
Camk2b	T	A	11: 5,922,838 (GRCm39)	N426Y	probably damaging	Het
Ccdc82	T	A	9: 13,289,706 (GRCm39)	Y500*	probably null	Het
Cenpe	T	A	3: 134,930,777 (GRCm39)	D341E	probably damaging	Het
Cyp2j13	A	T	4: 95,956,428 (GRCm39)	M227K	probably benign	Het
Dmbt1	G	A	7: 130,692,134 (GRCm39)	V967I	unknown	Het
Dnmt3b	A	T	2: 153,515,933 (GRCm39)	N447Y	probably benign	Het
Dtl	A	T	1: 191,285,175 (GRCm39)	Y320*	probably null	Het
Dtymk	A	C	1: 93,729,543 (GRCm39)	S3A	probably benign	Het
Eefsec	G	A	6: 88,423,253 (GRCm39)	T70M	possibly damaging	Het
Fam171a1	C	T	2: 3,221,344 (GRCm39)	T270M	probably damaging	Het
Gm17728	T	C	17: 9,641,159 (GRCm39)	S90P	probably benign	Het
Hdlbp	A	G	1: 93,359,053 (GRCm39)	L213P	probably damaging	Het
Heatr5b	T	C	17: 79,109,188 (GRCm39)	I1024V	probably benign	Het
Hr	C	T	14: 70,799,305 (GRCm39)	R597*	probably null	Het
Ifit3	T	C	19: 34,564,988 (GRCm39)	I178T	probably damaging	Het
Ifna6	A	G	4: 88,746,222 (GRCm39)	*190W	probably null	Het
Igkv4-55	A	T	6: 69,584,315 (GRCm39)	M99K	probably benign	Het
Klk1	T	A	7: 43,877,056 (GRCm39)	I53N	probably damaging	Het
Lpcat2b	A	G	5: 107,580,692 (GRCm39)	H7R	probably benign	Het
Lrrc37a	G	A	11: 103,393,481 (GRCm39)	P648L		Het
Mast4	G	T	13: 102,895,174 (GRCm39)	P975H	probably damaging	Het
Mfrp	C	T	9: 44,013,807 (GRCm39)	L106F	probably benign	Het
Mllt10	T	A	2: 18,201,469 (GRCm39)	S652T	probably benign	Het
Mroh8	C	A	2: 157,083,673 (GRCm39)	G400W	probably damaging	Het
Neurl4	A	G	11: 69,794,788 (GRCm39)		probably benign	Het
Nop14	A	C	5: 34,817,352 (GRCm39)	V13G	probably benign	Het
Nup188	T	A	2: 30,217,576 (GRCm39)	L837Q	probably damaging	Het
Or2a54	T	A	6: 43,092,905 (GRCm39)	N76K	possibly damaging	Het
Or2ak5	T	A	11: 58,611,572 (GRCm39)	T101S	probably benign	Het
Or7g30	T	C	9: 19,352,817 (GRCm39)	S203P	probably damaging	Het
Or8k39	T	C	2: 86,563,302 (GRCm39)	Y218C	probably damaging	Het
Pate10	A	G	9: 35,652,391 (GRCm39)	Y16C	probably damaging	Het
Pde8b	A	T	13: 95,226,793 (GRCm39)	V185E	probably benign	Het
Pink1	G	T	4: 138,047,333 (GRCm39)	A247D	probably damaging	Het
Pla2g10	A	T	16: 13,545,914 (GRCm39)	V40D	probably damaging	Het
Plce1	G	A	19: 38,512,811 (GRCm39)	G37R	probably benign	Het
Pld1	A	G	3: 28,166,439 (GRCm39)	E826G	possibly damaging	Het
Poc5	G	T	13: 96,535,228 (GRCm39)	K164N	possibly damaging	Het
Polr2b	A	T	5: 77,463,761 (GRCm39)	M53L	probably benign	Het
Ppp1r10	C	T	17: 36,239,690 (GRCm39)	P470S	probably damaging	Het
Rasef	A	G	4: 73,645,840 (GRCm39)	S510P	probably damaging	Het
Rnf17	T	A	14: 56,722,658 (GRCm39)	L1073Q	probably damaging	Het
Rpl13a-ps1	C	T	19: 50,018,739 (GRCm39)	G146R	probably damaging	Het
Scn2a	A	G	2: 65,518,730 (GRCm39)	D368G	probably damaging	Het
Shc3	T	A	13: 51,634,248 (GRCm39)	S86C	probably benign	Het
Sos1	T	C	17: 80,741,405 (GRCm39)	H460R	probably damaging	Het
Steap1	T	A	5: 5,790,838 (GRCm39)	M37L	probably benign	Het
Themis	G	T	10: 28,673,492 (GRCm39)	C593F	possibly damaging	Het
Trabd	G	T	15: 88,969,667 (GRCm39)		probably benign	Het
Trim42	T	A	9: 97,248,047 (GRCm39)	K216N	probably damaging	Het
Trmt44	G	A	5: 35,721,673 (GRCm39)	P497S	probably benign	Het
Trp53bp2	A	G	1: 182,256,475 (GRCm39)	Y15C	probably damaging	Het
Trpm7	T	A	2: 126,652,100 (GRCm39)	I1458L	probably benign	Het
Ttc17	G	T	2: 94,237,003 (GRCm39)	A3E	possibly damaging	Het
Ttn	C	A	2: 76,704,208 (GRCm39)	V9274L	unknown	Het
Tut7	T	A	13: 59,937,011 (GRCm39)	Q1212L	possibly damaging	Het
Vmn2r105	T	A	17: 20,428,872 (GRCm39)	I735F	probably damaging	Het
Vmn2r22	A	G	6: 123,614,454 (GRCm39)	C379R	probably damaging	Het
Vmn2r97	G	A	17: 19,149,607 (GRCm39)	E332K	probably benign	Het
Xrn1	A	G	9: 95,920,732 (GRCm39)	T1331A	probably benign	Het

Other mutations in Tnn

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Tnn	APN	1	159,953,021 (GRCm39)	missense	possibly damaging	0.65
IGL00433:Tnn	APN	1	159,925,776 (GRCm39)	splice site	probably benign
IGL00858:Tnn	APN	1	159,915,962 (GRCm39)	critical splice donor site	probably null
IGL00939:Tnn	APN	1	159,975,100 (GRCm39)	missense	probably damaging	1.00
IGL01569:Tnn	APN	1	159,948,124 (GRCm39)	missense	possibly damaging	0.51
IGL01591:Tnn	APN	1	159,953,144 (GRCm39)	missense	probably damaging	1.00
IGL01628:Tnn	APN	1	159,975,172 (GRCm39)	missense	possibly damaging	0.89
IGL01811:Tnn	APN	1	159,934,705 (GRCm39)	missense	probably damaging	1.00
IGL01813:Tnn	APN	1	159,916,008 (GRCm39)	missense	probably damaging	1.00
IGL02340:Tnn	APN	1	159,972,775 (GRCm39)	missense	probably benign	0.00
IGL02488:Tnn	APN	1	159,968,163 (GRCm39)	missense	probably benign	0.21
IGL02535:Tnn	APN	1	159,950,222 (GRCm39)	splice site	probably null
IGL02563:Tnn	APN	1	159,942,123 (GRCm39)	missense	probably damaging	1.00
IGL02572:Tnn	APN	1	159,913,677 (GRCm39)	missense	probably damaging	1.00
IGL02740:Tnn	APN	1	159,968,347 (GRCm39)	splice site	probably benign
IGL02818:Tnn	APN	1	159,943,848 (GRCm39)	missense	possibly damaging	0.86
IGL03284:Tnn	APN	1	159,953,022 (GRCm39)	missense	probably benign	0.01
1mM(1):Tnn	UTSW	1	159,924,911 (GRCm39)	missense	probably damaging	1.00
PIT4305001:Tnn	UTSW	1	159,913,647 (GRCm39)	missense	possibly damaging	0.91
R0023:Tnn	UTSW	1	159,932,498 (GRCm39)	missense	probably benign	0.00
R0234:Tnn	UTSW	1	159,916,036 (GRCm39)	missense	probably damaging	1.00
R0234:Tnn	UTSW	1	159,916,036 (GRCm39)	missense	probably damaging	1.00
R0316:Tnn	UTSW	1	159,948,137 (GRCm39)	missense	possibly damaging	0.93
R0492:Tnn	UTSW	1	159,948,327 (GRCm39)	missense	probably damaging	0.99
R0547:Tnn	UTSW	1	159,943,907 (GRCm39)	intron	probably benign
R1067:Tnn	UTSW	1	159,952,968 (GRCm39)	missense	probably damaging	1.00
R1563:Tnn	UTSW	1	159,952,985 (GRCm39)	missense	probably damaging	1.00
R1565:Tnn	UTSW	1	159,924,835 (GRCm39)	missense	probably damaging	1.00
R1615:Tnn	UTSW	1	159,945,978 (GRCm39)	missense	possibly damaging	0.93
R1637:Tnn	UTSW	1	159,975,170 (GRCm39)	missense	probably damaging	1.00
R1707:Tnn	UTSW	1	159,972,714 (GRCm39)	missense	probably damaging	1.00
R1758:Tnn	UTSW	1	159,975,154 (GRCm39)	missense	possibly damaging	0.61
R1797:Tnn	UTSW	1	159,968,258 (GRCm39)	missense	probably damaging	1.00
R1847:Tnn	UTSW	1	159,943,752 (GRCm39)	missense	possibly damaging	0.51
R1925:Tnn	UTSW	1	159,924,799 (GRCm39)	missense	probably damaging	1.00
R2182:Tnn	UTSW	1	159,968,170 (GRCm39)	splice site	probably null
R2196:Tnn	UTSW	1	159,924,798 (GRCm39)	nonsense	probably null
R2225:Tnn	UTSW	1	159,975,035 (GRCm39)	missense	probably damaging	1.00
R2227:Tnn	UTSW	1	159,975,035 (GRCm39)	missense	probably damaging	1.00
R2286:Tnn	UTSW	1	159,938,079 (GRCm39)	missense	possibly damaging	0.89
R2850:Tnn	UTSW	1	159,966,857 (GRCm39)	missense	probably benign	0.00
R3110:Tnn	UTSW	1	159,943,856 (GRCm39)	missense	possibly damaging	0.71
R3111:Tnn	UTSW	1	159,934,625 (GRCm39)	missense	probably damaging	0.98
R3112:Tnn	UTSW	1	159,943,856 (GRCm39)	missense	possibly damaging	0.71
R3729:Tnn	UTSW	1	159,973,810 (GRCm39)	missense	probably damaging	1.00
R4183:Tnn	UTSW	1	159,924,925 (GRCm39)	missense	probably damaging	1.00
R4439:Tnn	UTSW	1	159,943,650 (GRCm39)	missense	probably benign
R4441:Tnn	UTSW	1	159,943,650 (GRCm39)	missense	probably benign
R4588:Tnn	UTSW	1	159,972,681 (GRCm39)	missense	probably benign	0.25
R4646:Tnn	UTSW	1	159,973,612 (GRCm39)	missense	probably benign
R4647:Tnn	UTSW	1	159,973,612 (GRCm39)	missense	probably benign
R4648:Tnn	UTSW	1	159,973,612 (GRCm39)	missense	probably benign
R4701:Tnn	UTSW	1	159,975,338 (GRCm39)	missense	possibly damaging	0.72
R4703:Tnn	UTSW	1	159,943,815 (GRCm39)	missense	possibly damaging	0.84
R4737:Tnn	UTSW	1	159,973,659 (GRCm39)	missense	probably damaging	1.00
R4801:Tnn	UTSW	1	159,972,603 (GRCm39)	missense	possibly damaging	0.90
R4802:Tnn	UTSW	1	159,972,603 (GRCm39)	missense	possibly damaging	0.90
R4868:Tnn	UTSW	1	159,958,443 (GRCm39)	missense	possibly damaging	0.64
R4977:Tnn	UTSW	1	159,948,188 (GRCm39)	missense	probably damaging	1.00
R5011:Tnn	UTSW	1	159,953,949 (GRCm39)	missense	possibly damaging	0.89
R5026:Tnn	UTSW	1	159,973,707 (GRCm39)	missense	probably benign	0.00
R5027:Tnn	UTSW	1	159,972,781 (GRCm39)	missense	probably damaging	1.00
R5049:Tnn	UTSW	1	159,968,308 (GRCm39)	missense	probably benign	0.00
R5119:Tnn	UTSW	1	159,948,122 (GRCm39)	missense	probably damaging	0.98
R5128:Tnn	UTSW	1	159,950,464 (GRCm39)	missense	probably damaging	0.98
R5234:Tnn	UTSW	1	159,972,569 (GRCm39)	missense	possibly damaging	0.95
R5398:Tnn	UTSW	1	159,975,092 (GRCm39)	missense	probably benign	0.00
R5424:Tnn	UTSW	1	159,950,272 (GRCm39)	missense	possibly damaging	0.69
R5452:Tnn	UTSW	1	159,937,831 (GRCm39)	missense	probably benign	0.13
R5466:Tnn	UTSW	1	159,948,106 (GRCm39)	missense	possibly damaging	0.93
R6022:Tnn	UTSW	1	159,937,928 (GRCm39)	missense	probably benign	0.00
R6062:Tnn	UTSW	1	159,925,848 (GRCm39)	missense	probably damaging	1.00
R6086:Tnn	UTSW	1	159,913,690 (GRCm39)	missense	probably damaging	1.00
R6132:Tnn	UTSW	1	159,973,641 (GRCm39)	missense	probably damaging	0.96
R6324:Tnn	UTSW	1	159,972,774 (GRCm39)	missense	probably damaging	0.96
R6455:Tnn	UTSW	1	159,942,289 (GRCm39)	missense	probably damaging	1.00
R6563:Tnn	UTSW	1	159,915,968 (GRCm39)	missense	probably damaging	1.00
R6650:Tnn	UTSW	1	159,942,153 (GRCm39)	missense	probably damaging	1.00
R6806:Tnn	UTSW	1	159,948,278 (GRCm39)	missense	possibly damaging	0.95
R6810:Tnn	UTSW	1	159,932,412 (GRCm39)	missense	probably damaging	1.00
R7157:Tnn	UTSW	1	159,953,947 (GRCm39)	nonsense	probably null
R7243:Tnn	UTSW	1	159,934,687 (GRCm39)	missense	probably benign	0.07
R7340:Tnn	UTSW	1	159,973,592 (GRCm39)	missense	probably damaging	0.98
R7472:Tnn	UTSW	1	159,937,917 (GRCm39)	missense	probably benign	0.12
R7502:Tnn	UTSW	1	159,937,929 (GRCm39)	missense	probably benign	0.00
R7527:Tnn	UTSW	1	159,946,074 (GRCm39)	missense	possibly damaging	0.51
R7608:Tnn	UTSW	1	159,915,984 (GRCm39)	nonsense	probably null
R7746:Tnn	UTSW	1	159,942,255 (GRCm39)	missense	probably damaging	0.97
R8096:Tnn	UTSW	1	159,950,411 (GRCm39)	missense	probably damaging	1.00
R8136:Tnn	UTSW	1	159,934,630 (GRCm39)	missense	probably damaging	0.96
R8191:Tnn	UTSW	1	159,953,088 (GRCm39)	missense	probably damaging	1.00
R8334:Tnn	UTSW	1	159,946,053 (GRCm39)	missense	probably damaging	1.00
R8335:Tnn	UTSW	1	159,946,053 (GRCm39)	missense	probably damaging	1.00
R8337:Tnn	UTSW	1	159,946,053 (GRCm39)	missense	probably damaging	1.00
R8338:Tnn	UTSW	1	159,946,053 (GRCm39)	missense	probably damaging	1.00
R8427:Tnn	UTSW	1	159,958,256 (GRCm39)	missense	probably damaging	0.99
R8433:Tnn	UTSW	1	159,924,790 (GRCm39)	missense	possibly damaging	0.81
R8479:Tnn	UTSW	1	159,950,397 (GRCm39)	missense	probably benign	0.06
R8505:Tnn	UTSW	1	159,973,593 (GRCm39)	missense	probably damaging	0.98
R8554:Tnn	UTSW	1	159,937,986 (GRCm39)	missense	probably damaging	1.00
R8717:Tnn	UTSW	1	159,943,846 (GRCm39)	missense	possibly damaging	0.51
R8928:Tnn	UTSW	1	159,953,099 (GRCm39)	missense	probably damaging	1.00
R9209:Tnn	UTSW	1	159,953,986 (GRCm39)	missense	probably benign	0.02
X0019:Tnn	UTSW	1	159,913,716 (GRCm39)	missense	probably damaging	1.00
Z1176:Tnn	UTSW	1	159,973,863 (GRCm39)	missense	probably benign
Z1177:Tnn	UTSW	1	159,954,097 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GTCCTACCTAACCTGTCAAAGC -3'
(R):5'- ACATGGTTCGCTACACCTCTG -3'

Sequencing Primer
(F):5'- AAGTGCTTCACATGCATGCG -3'
(R):5'- TACACCTCTGCGGATGGAG -3'

Posted On

2021-07-15