Mutagenetix > Incidental Mutation

Incidental Mutation 'R8857:Or5ac25'

675439

Institutional Source

Beutler Lab

Gene Symbol

Or5ac25

Ensembl Gene

ENSMUSG00000095706

Gene Name

olfactory receptor family 5 subfamily AC member 25

Synonyms

MOR182-6, GA_x54KRFPKG5P-55580232-55579315, MOR182-6, Olfr207, MOR182-9, Olfr208, GA_x54KRFPKG5P-55570276-55569359, Olfr209, GA_x54KRFPKG5P-55590495-55589578

MMRRC Submission

068677-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.128) question?

Stock #

R8857 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

59181587-59182603 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 59182041 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 180 (I180N)

Ref Sequence

ENSEMBL: ENSMUSP00000147068 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055868] [ENSMUST00000208246] [ENSMUST00000208875] [ENSMUST00000213779]

AlphaFold

L7N1X3

Predicted Effect

probably damaging
Transcript: ENSMUST00000055868
AA Change: I180N

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000053192
Gene: ENSMUSG00000095706
AA Change: I180N

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	304	1.3e-49	PFAM
Pfam:7TM_GPCR_Srsx	34	303	1.3e-6	PFAM
Pfam:7tm_1	40	289	6.2e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000208246

Predicted Effect

probably damaging
Transcript: ENSMUST00000208875
AA Change: I180N

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

Predicted Effect

probably benign
Transcript: ENSMUST00000213779

Meta Mutation Damage Score

0.6329

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.4%

Validation Efficiency

98% (65/66)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ablim1	G	A	19: 57,119,287 (GRCm39)	L266F	possibly damaging	Het
Adgrf3	A	T	5: 30,402,065 (GRCm39)	C654*	probably null	Het
Adgrl1	C	A	8: 84,657,657 (GRCm39)	A421D	probably benign	Het
Adra1b	C	G	11: 43,727,092 (GRCm39)		probably benign	Het
Apba2	T	A	7: 64,399,939 (GRCm39)	V710E	possibly damaging	Het
Arhgef5	T	C	6: 43,264,558 (GRCm39)	V1523A	probably damaging	Het
AW551984	G	C	9: 39,511,831 (GRCm39)	A60G	probably damaging	Het
Bdh1	A	T	16: 31,265,450 (GRCm39)	T42S	probably benign	Het
C130073F10Rik	G	A	4: 101,747,555 (GRCm39)	P158L	possibly damaging	Het
Cacna2d4	A	T	6: 119,248,909 (GRCm39)	K457*	probably null	Het
Chd3	G	A	11: 69,253,146 (GRCm39)	P223L	probably benign	Het
Cnst	T	C	1: 179,437,878 (GRCm39)	S481P	probably damaging	Het
Col6a3	T	A	1: 90,703,485 (GRCm39)	K3027N	unknown	Het
Csf2rb2	T	C	15: 78,178,613 (GRCm39)	D111G	probably null	Het
Disc1	A	C	8: 125,891,870 (GRCm39)	E641A	probably damaging	Het
Fbln5	C	A	12: 101,726,990 (GRCm39)	C320F	probably damaging	Het
Fbn2	G	A	18: 58,286,933 (GRCm39)	T242I	probably damaging	Het
Frem1	T	A	4: 82,922,280 (GRCm39)		probably benign	Het
Gpsm1	G	C	2: 26,230,457 (GRCm39)	G469A	possibly damaging	Het
Hic1	A	G	11: 75,056,228 (GRCm39)	I887T	probably benign	Het
Hltf	T	C	3: 20,159,825 (GRCm39)	V692A	probably damaging	Het
Hsd17b13	A	G	5: 104,125,063 (GRCm39)	L40P	probably damaging	Het
Hydin	T	C	8: 111,298,587 (GRCm39)		probably null	Het
Irf2bpl	T	C	12: 86,929,359 (GRCm39)	Y438C	possibly damaging	Het
Itgb4	A	T	11: 115,871,853 (GRCm39)	N219I	probably benign	Het
Knop1	T	C	7: 118,451,949 (GRCm39)	K257E		Het
Krtap19-5	A	T	16: 88,693,139 (GRCm39)	F58I	unknown	Het
L3mbtl2	T	A	15: 81,571,320 (GRCm39)	F709L	unknown	Het
Map1a	G	A	2: 121,138,098 (GRCm39)	R2924H	probably damaging	Het
Mfsd13a	A	G	19: 46,356,567 (GRCm39)	D224G	probably benign	Het
Mme	A	T	3: 63,256,070 (GRCm39)	N510I	probably damaging	Het
Morc2a	T	A	11: 3,627,484 (GRCm39)		probably null	Het
Mptx2	T	C	1: 173,102,452 (GRCm39)	E79G	probably benign	Het
Nutm1	A	G	2: 112,081,523 (GRCm39)	M380T	probably benign	Het
P2rx1	A	G	11: 72,903,197 (GRCm39)		probably benign	Het
Padi4	A	G	4: 140,501,472 (GRCm39)	I7T	probably damaging	Het
Phf20l1	T	C	15: 66,513,781 (GRCm39)	S1013P	probably benign	Het
Plb1	T	C	5: 32,521,556 (GRCm39)	V1469A	unknown	Het
Polr1g	C	T	7: 19,093,355 (GRCm39)		probably benign	Het
Prkab1	T	C	5: 116,158,147 (GRCm39)	N150D	probably damaging	Het
Qsox1	A	G	1: 155,658,333 (GRCm39)	V412A	possibly damaging	Het
Rai1	A	G	11: 60,077,393 (GRCm39)	S486G	probably benign	Het
Rev3l	T	A	10: 39,670,965 (GRCm39)	Y170*	probably null	Het
Rims2	T	A	15: 39,543,044 (GRCm39)	M1426K	possibly damaging	Het
Selenoi	C	A	5: 30,461,160 (GRCm39)	S132*	probably null	Het
Shtn1	T	C	19: 58,978,800 (GRCm39)	I498M	probably damaging	Het
Sin3b	T	C	8: 73,483,523 (GRCm39)	M970T	probably benign	Het
Slc17a8	A	G	10: 89,427,022 (GRCm39)	F360L	probably damaging	Het
Slc7a11	A	G	3: 50,393,305 (GRCm39)	Y113H	probably damaging	Het
Spast	T	A	17: 74,675,938 (GRCm39)	M327K	possibly damaging	Het
Sphkap	T	A	1: 83,258,288 (GRCm39)	I152F	probably damaging	Het
Tecpr1	T	C	5: 144,153,117 (GRCm39)	E204G	possibly damaging	Het
Tiam1	A	T	16: 89,662,145 (GRCm39)	S658T	probably damaging	Het
Tmem174	G	T	13: 98,773,433 (GRCm39)	H132Q	probably damaging	Het
Tnfrsf10b	G	A	14: 70,012,543 (GRCm39)	V117I	probably benign	Het
Tnr	A	G	1: 159,713,728 (GRCm39)	T719A	probably benign	Het
Tomm34	G	A	2: 163,896,379 (GRCm39)	P292S	probably damaging	Het
Trav15-1-dv6-1	T	A	14: 53,797,615 (GRCm39)	F88Y	probably damaging	Het
Trp63	A	T	16: 25,639,226 (GRCm39)	H138L	probably damaging	Het
U2af2	T	A	7: 5,065,290 (GRCm39)	S2T	probably damaging	Het
Uroc1	T	C	6: 90,334,510 (GRCm39)	V574A	possibly damaging	Het
Zc3h7b	G	A	15: 81,656,681 (GRCm39)	R166Q	probably benign	Het
Zfp488	A	C	14: 33,692,760 (GRCm39)	S134R	probably benign	Het
Zfp950	T	C	19: 61,116,001 (GRCm39)	D2G	probably benign	Het
Zfyve1	T	A	12: 83,598,374 (GRCm39)	H618L	probably damaging	Het
Zp1	T	G	19: 10,893,888 (GRCm39)	D439A	probably damaging	Het

Other mutations in Or5ac25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01083:Or5ac25	APN	16	59,182,198 (GRCm39)	missense	probably damaging	1.00
IGL01137:Or5ac25	APN	16	59,182,335 (GRCm39)	missense	probably benign	0.01
IGL01383:Or5ac25	APN	16	59,182,316 (GRCm39)	missense	probably benign	0.10
IGL01913:Or5ac25	APN	16	59,182,294 (GRCm39)	missense	probably damaging	1.00
IGL01914:Or5ac25	APN	16	59,182,294 (GRCm39)	missense	probably damaging	1.00
IGL02125:Or5ac25	APN	16	59,181,879 (GRCm39)	missense	probably benign
IGL02414:Or5ac25	APN	16	59,182,077 (GRCm39)	missense	probably damaging	1.00
IGL02512:Or5ac25	APN	16	59,182,171 (GRCm39)	missense	possibly damaging	0.95
PIT4495001:Or5ac25	UTSW	16	59,181,871 (GRCm39)	missense	probably damaging	0.99
R0029:Or5ac25	UTSW	16	59,181,904 (GRCm39)	missense	probably benign	0.00
R0531:Or5ac25	UTSW	16	59,182,171 (GRCm39)	missense	probably damaging	0.99
R1799:Or5ac25	UTSW	16	59,182,243 (GRCm39)	missense	probably benign	0.01
R1901:Or5ac25	UTSW	16	59,182,526 (GRCm39)	missense	probably benign	0.03
R1902:Or5ac25	UTSW	16	59,182,526 (GRCm39)	missense	probably benign	0.03
R1903:Or5ac25	UTSW	16	59,182,526 (GRCm39)	missense	probably benign	0.03
R1972:Or5ac25	UTSW	16	59,182,476 (GRCm39)	missense	probably damaging	0.96
R2471:Or5ac25	UTSW	16	59,181,944 (GRCm39)	missense	possibly damaging	0.79
R5013:Or5ac25	UTSW	16	59,182,067 (GRCm39)	missense	probably damaging	1.00
R5160:Or5ac25	UTSW	16	59,182,129 (GRCm39)	missense	probably damaging	1.00
R5883:Or5ac25	UTSW	16	59,182,078 (GRCm39)	missense	probably damaging	1.00
R6272:Or5ac25	UTSW	16	59,181,948 (GRCm39)	missense	possibly damaging	0.76
R6296:Or5ac25	UTSW	16	59,181,769 (GRCm39)	missense	probably benign	0.06
R6741:Or5ac25	UTSW	16	59,181,918 (GRCm39)	missense	probably damaging	1.00
R6928:Or5ac25	UTSW	16	59,181,826 (GRCm39)	missense	probably damaging	1.00
R7982:Or5ac25	UTSW	16	59,181,927 (GRCm39)	missense	probably benign
R8254:Or5ac25	UTSW	16	59,182,534 (GRCm39)	missense	probably benign	0.00
R8429:Or5ac25	UTSW	16	59,181,990 (GRCm39)	missense	possibly damaging	0.89
R9596:Or5ac25	UTSW	16	59,181,942 (GRCm39)	missense	possibly damaging	0.50
R9611:Or5ac25	UTSW	16	59,182,242 (GRCm39)	missense	probably benign	0.04
R9658:Or5ac25	UTSW	16	59,182,106 (GRCm39)	missense	probably damaging	1.00
X0017:Or5ac25	UTSW	16	59,182,002 (GRCm39)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- TAGAGAAGGCTTTGTTTCTGCCC -3'
(R):5'- AGTGCAACTGCGGAATGTTTC -3'

Sequencing Primer
(F):5'- CCTCTCAGACTTTGTTCTCAGGATGG -3'
(R):5'- ATGACCGCTATGTAGCCATATGC -3'

Posted On

2021-07-15