Mutagenetix > Incidental Mutation

Incidental Mutation 'R8862:Or6d13'

675710

Institutional Source

Beutler Lab

Gene Symbol

Or6d13

Ensembl Gene

ENSMUSG00000081649

Gene Name

olfactory receptor family 6 subfamily D member 13

Synonyms

GA_x54KRFPKN04-58174409-58175392, Olfr213, MOR119-3

MMRRC Submission

068741-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R8862 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

116517416-116518399 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 116518186 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Isoleucine at position 257 (M257I)

Ref Sequence

ENSEMBL: ENSMUSP00000151916 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000121958] [ENSMUST00000220008]

AlphaFold

Q7TS32

Predicted Effect

probably benign
Transcript: ENSMUST00000121958
AA Change: M257I

PolyPhen 2 Score 0.026 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000112931
Gene: ENSMUSG00000081649
AA Change: M257I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	37	314	4e-45	PFAM
Pfam:7tm_1	47	296	5.4e-15	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000220008
AA Change: M257I

PolyPhen 2 Score 0.026 (Sensitivity: 0.95; Specificity: 0.81)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (61/61)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acin1	C	T	14: 54,901,172 (GRCm39)	G685R	probably benign	Het
Ahdc1	T	C	4: 132,791,129 (GRCm39)	F790S	possibly damaging	Het
Alg10b	T	C	15: 90,109,893 (GRCm39)	Y69H	probably damaging	Het
Amer3	A	T	1: 34,626,465 (GRCm39)	S235C	probably damaging	Het
Ankfy1	G	T	11: 72,644,469 (GRCm39)	S722I	probably benign	Het
Baz1a	T	C	12: 55,032,624 (GRCm39)		probably benign	Het
Ccdc177	A	G	12: 80,804,208 (GRCm39)	S689P	unknown	Het
Cdh24	C	A	14: 54,869,874 (GRCm39)	R681L	probably damaging	Het
Cdk14	T	G	5: 5,060,862 (GRCm39)	I338L	probably benign	Het
Clec2m	T	C	6: 129,308,494 (GRCm39)	T12A	probably benign	Het
Col18a1	G	T	10: 76,949,044 (GRCm39)	S156*	probably null	Het
Col6a6	T	C	9: 105,663,348 (GRCm39)	Y63C	probably damaging	Het
Cytip	G	A	2: 58,037,887 (GRCm39)	T159M	probably benign	Het
Dnah5	T	C	15: 28,459,502 (GRCm39)		probably benign	Het
Dock3	T	C	9: 106,855,927 (GRCm39)	Y744C	probably damaging	Het
Dop1a	T	C	9: 86,406,404 (GRCm39)		probably null	Het
Dse	A	T	10: 34,029,934 (GRCm39)	D385E	probably damaging	Het
Efhc1	A	C	1: 21,037,573 (GRCm39)	D250A		Het
Epha3	T	A	16: 63,431,348 (GRCm39)	T519S	probably benign	Het
Gas2l3	A	G	10: 89,250,282 (GRCm39)	Y279H	probably damaging	Het
Gatd3a	A	G	10: 78,005,461 (GRCm39)	S35P	probably damaging	Het
Gemin6	G	A	17: 80,535,432 (GRCm39)	V131I	probably damaging	Het
Gm40460	CACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	CACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	7: 141,794,554 (GRCm39)		probably benign	Het
Gnai2	C	A	9: 107,512,326 (GRCm39)	A31S		Het
Greb1l	A	G	18: 10,555,042 (GRCm39)	D1696G	possibly damaging	Het
Itfg2	T	C	6: 128,394,668 (GRCm39)	E35G	probably damaging	Het
Kctd10	A	G	5: 114,503,921 (GRCm39)	F244L	probably damaging	Het
Kif19b	C	A	5: 140,472,534 (GRCm39)	P686Q	probably damaging	Het
Krtap5-1	ACAGGGCTTGCAGCAGCTGGACTGACAGCAGCAGGGCTTGCAGCAGCTGGACTGACAGCAGCAGGGCTTGCAGCAGCTGGACTGACAGCAG	ACAGGGCTTGCAGCAGCTGGACTGACAGCAGCAGGGCTTGCAGCAGCTGGACTGACAGCAG	7: 141,850,333 (GRCm39)		probably benign	Het
Lgals9	G	T	11: 78,860,716 (GRCm39)		probably benign	Het
Lrriq4	A	T	3: 30,705,088 (GRCm39)	N372I	probably damaging	Het
Mical2	A	G	7: 111,910,574 (GRCm39)	N248S	probably damaging	Het
Mipep	T	A	14: 61,080,689 (GRCm39)	C560*	probably null	Het
Myo1a	G	A	10: 127,548,653 (GRCm39)	V396M	probably benign	Het
Myod1	A	G	7: 46,026,487 (GRCm39)	T131A	probably damaging	Het
Nalcn	T	A	14: 123,647,199 (GRCm39)	D558V	possibly damaging	Het
Odf2l	T	A	3: 144,833,758 (GRCm39)		probably benign	Het
Plbd2	A	T	5: 120,624,728 (GRCm39)	M480K	probably damaging	Het
Ppargc1a	A	G	5: 51,631,235 (GRCm39)	F465L	possibly damaging	Het
Ppp1r15b	A	T	1: 133,064,506 (GRCm39)	I659F	probably damaging	Het
Prune2	C	T	19: 17,097,510 (GRCm39)	L1005F	probably benign	Het
Pter	T	C	2: 12,985,341 (GRCm39)	S224P	probably damaging	Het
Ralgapa2	A	G	2: 146,266,731 (GRCm39)	I615T	probably benign	Het
Ror1	T	C	4: 100,191,715 (GRCm39)		probably null	Het
Rps2	A	G	17: 24,940,662 (GRCm39)	T259A	probably benign	Het
Rsf1	GCG	GCGACGGCGCCG	7: 97,229,114 (GRCm39)		probably benign	Het
S1pr4	G	A	10: 81,334,533 (GRCm39)	R314C	probably damaging	Het
Sema4f	A	T	6: 82,891,081 (GRCm39)	H575Q	probably benign	Het
Son	A	G	16: 91,453,734 (GRCm39)	D827G	probably damaging	Het
Sox8	A	T	17: 25,787,045 (GRCm39)	H219Q	possibly damaging	Het
Spink2	C	T	5: 77,357,615 (GRCm39)	D24N	probably benign	Het
Stard9	A	G	2: 120,534,099 (GRCm39)	D3452G	probably benign	Het
Syngr2	G	T	11: 117,703,507 (GRCm39)	D108Y	probably damaging	Het
Tardbp	G	A	4: 148,702,755 (GRCm39)	S403L	possibly damaging	Het
Tomm70a	T	C	16: 56,942,546 (GRCm39)	S108P	probably benign	Het
Ttc12	T	C	9: 49,351,515 (GRCm39)	T661A	probably benign	Het
Tubgcp6	A	G	15: 89,006,824 (GRCm39)	V66A	probably benign	Het
Unc13b	T	A	4: 43,235,207 (GRCm39)	L477Q	probably damaging	Het
Vmn1r77	T	A	7: 11,776,060 (GRCm39)	C279S	probably benign	Het
Vmn2r79	T	C	7: 86,645,712 (GRCm39)	S14P	probably benign	Het
Zan	T	C	5: 137,472,674 (GRCm39)	T72A	probably benign	Het
Zcchc2	A	G	1: 105,958,998 (GRCm39)	*1156W	probably null	Het
Zfp354c	A	T	11: 50,708,718 (GRCm39)	D26E	probably benign	Het

Other mutations in Or6d13

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01063:Or6d13	APN	6	116,517,968 (GRCm39)	missense	probably damaging	1.00
IGL02671:Or6d13	APN	6	116,518,104 (GRCm39)	missense	possibly damaging	0.50
IGL03107:Or6d13	APN	6	116,517,900 (GRCm39)	missense	possibly damaging	0.82
R0041:Or6d13	UTSW	6	116,518,295 (GRCm39)	missense	possibly damaging	0.55
R0426:Or6d13	UTSW	6	116,517,446 (GRCm39)	missense	probably damaging	1.00
R0627:Or6d13	UTSW	6	116,517,949 (GRCm39)	missense	possibly damaging	0.56
R0829:Or6d13	UTSW	6	116,518,226 (GRCm39)	missense	probably benign	0.01
R2111:Or6d13	UTSW	6	116,517,611 (GRCm39)	missense	possibly damaging	0.95
R2112:Or6d13	UTSW	6	116,517,611 (GRCm39)	missense	possibly damaging	0.95
R2120:Or6d13	UTSW	6	116,517,416 (GRCm39)	start codon destroyed	probably null	0.04
R3161:Or6d13	UTSW	6	116,517,807 (GRCm39)	missense	probably damaging	1.00
R3700:Or6d13	UTSW	6	116,517,489 (GRCm39)	missense	probably benign	0.10
R4710:Or6d13	UTSW	6	116,517,611 (GRCm39)	missense	possibly damaging	0.95
R5244:Or6d13	UTSW	6	116,518,187 (GRCm39)	missense	probably damaging	1.00
R5859:Or6d13	UTSW	6	116,517,861 (GRCm39)	missense	probably damaging	1.00
R6273:Or6d13	UTSW	6	116,518,277 (GRCm39)	missense	possibly damaging	0.74
R6505:Or6d13	UTSW	6	116,517,561 (GRCm39)	missense	probably benign	0.01
R7350:Or6d13	UTSW	6	116,517,495 (GRCm39)	missense	probably benign	0.28
R7380:Or6d13	UTSW	6	116,517,894 (GRCm39)	missense	probably benign	0.03
R7474:Or6d13	UTSW	6	116,517,999 (GRCm39)	missense	probably damaging	0.99
R8054:Or6d13	UTSW	6	116,517,960 (GRCm39)	missense	probably damaging	1.00
R8476:Or6d13	UTSW	6	116,517,507 (GRCm39)	missense	probably benign
R9082:Or6d13	UTSW	6	116,517,969 (GRCm39)	missense	probably damaging	1.00
R9100:Or6d13	UTSW	6	116,517,990 (GRCm39)	missense	possibly damaging	0.93
R9124:Or6d13	UTSW	6	116,517,416 (GRCm39)	start codon destroyed	probably null	0.04
R9151:Or6d13	UTSW	6	116,517,990 (GRCm39)	missense	possibly damaging	0.93
R9321:Or6d13	UTSW	6	116,518,370 (GRCm39)	missense	probably benign
R9404:Or6d13	UTSW	6	116,517,708 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACTGGCAAATCTCTCCTGTTCAG -3'
(R):5'- ACTGCTCCTGTGATATCTGGG -3'

Sequencing Primer
(F):5'- CAGATACCATGTCTGTTGAGATGC -3'
(R):5'- GTAATTGAACCCTGGACAGTGCATC -3'

Posted On

2021-07-15