Mutagenetix > Incidental Mutation

Incidental Mutation 'R8865:Notch3'

675895

Institutional Source

Beutler Lab

Gene Symbol

Notch3

Ensembl Gene

ENSMUSG00000038146

Gene Name

notch 3

Synonyms

hpbk, N3

MMRRC Submission

068681-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8865 (G1)

Quality Score

187.009

Status

Validated

Chromosome

Chromosomal Location

32339794-32385826 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 32341090 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 2221 (Y2221H)

Ref Sequence

ENSEMBL: ENSMUSP00000085016 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087723]

AlphaFold

Q61982

Predicted Effect

probably benign
Transcript: ENSMUST00000087723
AA Change: Y2221H

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000085016
Gene: ENSMUSG00000038146
AA Change: Y2221H

Domain	Start	End	E-Value	Type
signal peptide	1	39	N/A	INTRINSIC
EGF	42	78	3.73e-5	SMART
EGF	82	119	1.78e-2	SMART
EGF	123	157	1.13e-4	SMART
EGF_CA	159	196	1.89e-15	SMART
EGF	201	235	3.85e-7	SMART
EGF_CA	237	273	3.97e-9	SMART
EGF_CA	275	313	4.63e-10	SMART
EGF_CA	315	351	1.05e-8	SMART
EGF	355	390	1.28e-3	SMART
EGF_CA	392	430	6.29e-12	SMART
EGF_CA	432	468	1.11e-12	SMART
EGF_CA	470	506	4.21e-13	SMART
EGF_CA	508	544	8.43e-13	SMART
EGF_CA	546	581	9.54e-12	SMART
EGF_CA	583	619	1.31e-9	SMART
EGF_CA	621	656	2.03e-6	SMART
EGF_CA	658	694	2.28e-9	SMART
EGF	699	731	7.18e-7	SMART
EGF	738	771	2.5e-6	SMART
EGF	775	809	8e-5	SMART
EGF_CA	811	848	4.77e-12	SMART
EGF_CA	850	886	3.81e-11	SMART
EGF_CA	888	923	1.47e-12	SMART
EGF_CA	925	961	3.4e-8	SMART
EGF	966	999	5.74e-6	SMART
EGF	1004	1035	7.18e-7	SMART
EGF	1040	1083	1.21e-4	SMART
EGF_CA	1085	1121	1.29e-8	SMART
EGF_CA	1123	1159	1.45e-11	SMART
EGF_CA	1161	1204	1.26e-11	SMART
EGF	1209	1245	1.53e-1	SMART
EGF	1250	1288	8e-5	SMART
EGF	1293	1326	1.13e1	SMART
EGF	1339	1374	5.36e-6	SMART
NL	1381	1419	1.63e-15	SMART
NL	1422	1460	1.78e-16	SMART
NL	1461	1502	1.75e-15	SMART
NOD	1506	1562	2.98e-24	SMART
NODP	1577	1641	1.34e-26	SMART
transmembrane domain	1644	1666	N/A	INTRINSIC
low complexity region	1774	1783	N/A	INTRINSIC
ANK	1789	1834	1.48e3	SMART
ANK	1839	1868	1.61e-4	SMART
ANK	1872	1902	1.42e0	SMART
ANK	1906	1935	1.77e-1	SMART
ANK	1939	1968	3.18e-3	SMART
ANK	1972	2001	5.16e-3	SMART
low complexity region	2028	2054	N/A	INTRINSIC
low complexity region	2108	2123	N/A	INTRINSIC
low complexity region	2169	2193	N/A	INTRINSIC
DUF3454	2218	2275	2.81e-20	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

100% (72/72)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008]
PHENOTYPE: Some, but not all, null alleles cause defects in artery morphology and in T cell development. Progressive emaciation and kyphosis with paraphimosis occurs in an intron 31 splice donor site point mutant. In conjunction with Notch1 deficiency, abnormalities in embryonic development have been observed. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts2	T	A	11: 50,672,571 (GRCm39)	Y606*	probably null	Het
Adgrb1	A	G	15: 74,415,507 (GRCm39)	I696V	possibly damaging	Het
Ass1	A	G	2: 31,410,407 (GRCm39)	Q406R	probably benign	Het
Calcoco2	GGGCCTTCTCTTTCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTTCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTTCTCCCAGGAGGCCTTCTCTTCC	GGGCCTTCTCTTTCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTCCTCCCAGGAGGCCTTCTCTTTCTCCCAGGAGGCCTTCTCTTCC	11: 95,990,808 (GRCm39)		probably benign	Het
Ccdc148	T	C	2: 58,719,832 (GRCm39)	K409R	possibly damaging	Het
Ccnl1	A	T	3: 65,854,269 (GRCm39)	S451T	probably benign	Het
Cdc123	T	C	2: 5,800,235 (GRCm39)		probably benign	Het
Cep192	T	C	18: 67,967,703 (GRCm39)	V729A	probably benign	Het
Chd6	G	A	2: 160,862,989 (GRCm39)	A444V	probably benign	Het
Chl1	A	G	6: 103,685,822 (GRCm39)	K898E	probably damaging	Het
Chrng	T	C	1: 87,135,219 (GRCm39)	V154A	probably damaging	Het
Cog1	T	G	11: 113,549,324 (GRCm39)	M799R	probably benign	Het
Col4a3	G	A	1: 82,647,483 (GRCm39)		probably null	Het
Coro6	C	A	11: 77,359,917 (GRCm39)	T366K	probably damaging	Het
Cwh43	G	A	5: 73,598,702 (GRCm39)	M640I	probably benign	Het
Cyp2c55	A	G	19: 39,019,878 (GRCm39)	E272G	probably benign	Het
Cyp4f39	A	G	17: 32,702,271 (GRCm39)	Y256C	probably damaging	Het
Dbx2	G	A	15: 95,530,281 (GRCm39)	R229*	probably null	Het
Dcdc2a	C	T	13: 25,386,266 (GRCm39)	A380V	probably benign	Het
En1	G	T	1: 120,530,729 (GRCm39)		probably benign	Het
F3	T	A	3: 121,523,060 (GRCm39)	V90E	probably damaging	Het
Fam171a1	A	G	2: 3,226,940 (GRCm39)	K691R	probably damaging	Het
Foxp2	C	T	6: 15,415,093 (GRCm39)	A609V	unknown	Het
Hemgn	A	T	4: 46,396,682 (GRCm39)	S185T	possibly damaging	Het
Hk1	C	T	10: 62,151,294 (GRCm39)	D33N	probably benign	Het
Igfbp1	T	A	11: 7,151,929 (GRCm39)	V244D	probably damaging	Het
Insr	A	T	8: 3,211,358 (GRCm39)	D1160E	probably damaging	Het
Irs1	A	T	1: 82,265,830 (GRCm39)	Y795*	probably null	Het
Krtap28-10	T	C	1: 83,019,808 (GRCm39)	K111E	unknown	Het
Lbx1	T	G	19: 45,223,605 (GRCm39)	D21A	probably benign	Het
Map6	G	T	7: 98,918,192 (GRCm39)	A322S	probably benign	Het
Mat1a	T	C	14: 40,843,788 (GRCm39)	Y336H	probably damaging	Het
Mcf2l	A	G	8: 12,930,003 (GRCm39)	I8V	probably benign	Het
Med12l	T	C	3: 58,979,303 (GRCm39)	V276A	probably benign	Het
Mettl17	A	G	14: 52,122,308 (GRCm39)		probably benign	Het
Mllt1	T	C	17: 57,207,295 (GRCm39)	D183G	possibly damaging	Het
Msmb	T	A	14: 31,872,217 (GRCm39)	C69*	probably null	Het
Mterf1a	A	G	5: 3,941,425 (GRCm39)	S148P	probably damaging	Het
Mybl2	A	G	2: 162,922,653 (GRCm39)	I583V	probably benign	Het
Nbr1	T	A	11: 101,455,520 (GRCm39)	D91E	probably benign	Het
Npat	C	A	9: 53,481,940 (GRCm39)	T1216N	probably benign	Het
Or14j6	T	C	17: 38,215,115 (GRCm39)	L226P	probably damaging	Het
Or2j3	T	A	17: 38,615,872 (GRCm39)	H160L	probably damaging	Het
Or8k35	C	A	2: 86,424,744 (GRCm39)	V143F	possibly damaging	Het
Pawr	T	A	10: 108,218,603 (GRCm39)	Y170N	probably damaging	Het
Pde12	T	C	14: 26,390,280 (GRCm39)	D143G	possibly damaging	Het
Phactr2	A	G	10: 13,129,476 (GRCm39)	I264T	probably benign	Het
Pip5k1b	A	G	19: 24,374,422 (GRCm39)	L53P	probably damaging	Het
Pknox1	T	C	17: 31,818,520 (GRCm39)	I251T	probably benign	Het
Plcxd1	A	T	5: 110,249,841 (GRCm39)		probably benign	Het
Polr3c	C	T	3: 96,622,517 (GRCm39)		probably benign	Het
Pramel4	G	C	4: 143,795,052 (GRCm39)	G483R	probably damaging	Het
Prdm10	T	C	9: 31,238,693 (GRCm39)	L195P	probably damaging	Het
Prss16	A	T	13: 22,187,175 (GRCm39)	L465Q	possibly damaging	Het
Psg25	G	T	7: 18,263,519 (GRCm39)	H101Q	possibly damaging	Het
Pstpip2	A	G	18: 77,934,108 (GRCm39)	D55G	possibly damaging	Het
Rfc1	A	T	5: 65,436,135 (GRCm39)	D636E	possibly damaging	Het
Scarf2	G	T	16: 17,620,974 (GRCm39)	C214F	probably damaging	Het
Sirt4	T	C	5: 115,620,704 (GRCm39)	E156G	probably damaging	Het
Spag6	C	T	2: 18,738,928 (GRCm39)	S286L	probably benign	Het
Stx17	T	A	4: 48,183,444 (GRCm39)	H267Q	unknown	Het
Tekt3	T	C	11: 62,961,058 (GRCm39)	C76R	probably benign	Het
Tln1	A	T	4: 43,538,281 (GRCm39)	V1807E	possibly damaging	Het
Tnfrsf21	A	G	17: 43,396,372 (GRCm39)	D552G	probably damaging	Het
Ttn	C	A	2: 76,560,664 (GRCm39)	V29246L	possibly damaging	Het
Usp43	C	A	11: 67,789,788 (GRCm39)	C252F	probably damaging	Het
Vmn2r16	T	C	5: 109,487,910 (GRCm39)	I261T	probably benign	Het
Vwa5b1	T	C	4: 138,308,530 (GRCm39)	E769G	probably benign	Het
Xrn1	T	A	9: 95,873,246 (GRCm39)	F670Y	probably benign	Het
Ypel1	T	C	16: 16,915,269 (GRCm39)	N113S	probably benign	Het
Zc3h7b	G	A	15: 81,656,681 (GRCm39)	R166Q	probably benign	Het
Zdhhc14	A	T	17: 5,775,570 (GRCm39)	Y274F	possibly damaging	Het
Zeb2	T	A	2: 44,886,139 (GRCm39)	M973L	probably benign	Het
Zfp638	T	C	6: 83,954,035 (GRCm39)	V1380A	possibly damaging	Het

Other mutations in Notch3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00161:Notch3	APN	17	32,377,088 (GRCm39)	nonsense	probably null
IGL01065:Notch3	APN	17	32,365,390 (GRCm39)	nonsense	probably null
IGL01296:Notch3	APN	17	32,385,731 (GRCm39)	missense	unknown
IGL01322:Notch3	APN	17	32,363,445 (GRCm39)	missense	probably damaging	1.00
IGL01343:Notch3	APN	17	32,362,410 (GRCm39)	missense	probably benign	0.10
IGL01358:Notch3	APN	17	32,363,721 (GRCm39)	missense	probably damaging	1.00
IGL01600:Notch3	APN	17	32,363,472 (GRCm39)	missense	probably damaging	1.00
IGL01622:Notch3	APN	17	32,377,844 (GRCm39)	missense	possibly damaging	0.50
IGL01623:Notch3	APN	17	32,377,844 (GRCm39)	missense	possibly damaging	0.50
IGL01971:Notch3	APN	17	32,343,321 (GRCm39)	missense	probably damaging	1.00
IGL02000:Notch3	APN	17	32,341,716 (GRCm39)	missense	probably damaging	0.99
IGL02072:Notch3	APN	17	32,366,048 (GRCm39)	nonsense	probably null
IGL02145:Notch3	APN	17	32,373,715 (GRCm39)	missense	probably benign	0.01
IGL02256:Notch3	APN	17	32,351,298 (GRCm39)	missense	probably damaging	1.00
IGL02366:Notch3	APN	17	32,363,179 (GRCm39)	missense	probably benign
IGL02476:Notch3	APN	17	32,377,612 (GRCm39)	missense	possibly damaging	0.67
IGL02502:Notch3	APN	17	32,377,252 (GRCm39)	nonsense	probably null
IGL02551:Notch3	APN	17	32,373,705 (GRCm39)	splice site	probably benign
divide	UTSW	17	32,356,787 (GRCm39)	splice site	probably null
impressed	UTSW	17	32,385,652 (GRCm39)	missense	probably benign
indented	UTSW	17	32,366,937 (GRCm39)	missense	probably benign	0.00
Lopressor	UTSW	17	32,372,858 (GRCm39)	missense	probably damaging	1.00
marginal	UTSW	17	32,383,198 (GRCm39)	missense	probably benign
PIT4486001:Notch3	UTSW	17	32,373,737 (GRCm39)	missense	probably damaging	1.00
R0115:Notch3	UTSW	17	32,352,436 (GRCm39)	missense	possibly damaging	0.82
R0201:Notch3	UTSW	17	32,375,122 (GRCm39)	splice site	probably benign
R0630:Notch3	UTSW	17	32,366,446 (GRCm39)	splice site	probably benign
R1167:Notch3	UTSW	17	32,341,719 (GRCm39)	missense	possibly damaging	0.95
R1432:Notch3	UTSW	17	32,383,198 (GRCm39)	missense	probably benign
R1567:Notch3	UTSW	17	32,377,554 (GRCm39)	missense	possibly damaging	0.77
R1623:Notch3	UTSW	17	32,358,165 (GRCm39)	missense	probably benign	0.00
R1663:Notch3	UTSW	17	32,375,093 (GRCm39)	missense	probably damaging	1.00
R1668:Notch3	UTSW	17	32,377,563 (GRCm39)	missense	probably damaging	0.99
R1789:Notch3	UTSW	17	32,377,699 (GRCm39)	missense	probably damaging	1.00
R1813:Notch3	UTSW	17	32,362,402 (GRCm39)	missense	probably benign	0.08
R1837:Notch3	UTSW	17	32,343,296 (GRCm39)	missense	probably damaging	1.00
R1896:Notch3	UTSW	17	32,362,402 (GRCm39)	missense	probably benign	0.08
R1937:Notch3	UTSW	17	32,372,826 (GRCm39)	missense	probably benign	0.03
R1954:Notch3	UTSW	17	32,385,652 (GRCm39)	missense	probably benign
R2014:Notch3	UTSW	17	32,376,974 (GRCm39)	missense	probably benign	0.00
R2058:Notch3	UTSW	17	32,362,618 (GRCm39)	missense	probably benign
R2068:Notch3	UTSW	17	32,354,482 (GRCm39)	missense	probably benign	0.00
R2097:Notch3	UTSW	17	32,341,728 (GRCm39)	missense	probably damaging	1.00
R2112:Notch3	UTSW	17	32,363,584 (GRCm39)	missense	probably benign	0.19
R2156:Notch3	UTSW	17	32,366,818 (GRCm39)	missense	probably damaging	1.00
R2211:Notch3	UTSW	17	32,366,952 (GRCm39)	missense	probably benign	0.00
R2324:Notch3	UTSW	17	32,369,108 (GRCm39)	splice site	probably benign
R2432:Notch3	UTSW	17	32,372,778 (GRCm39)	missense	probably damaging	1.00
R3117:Notch3	UTSW	17	32,377,089 (GRCm39)	missense	probably damaging	1.00
R3236:Notch3	UTSW	17	32,377,435 (GRCm39)	missense	probably damaging	0.96
R3409:Notch3	UTSW	17	32,369,676 (GRCm39)	missense	possibly damaging	0.67
R3434:Notch3	UTSW	17	32,377,592 (GRCm39)	missense	possibly damaging	0.80
R3435:Notch3	UTSW	17	32,377,592 (GRCm39)	missense	possibly damaging	0.80
R3438:Notch3	UTSW	17	32,372,564 (GRCm39)	missense	probably damaging	1.00
R3926:Notch3	UTSW	17	32,372,531 (GRCm39)	missense	possibly damaging	0.92
R4087:Notch3	UTSW	17	32,377,087 (GRCm39)	missense	possibly damaging	0.60
R4115:Notch3	UTSW	17	32,377,407 (GRCm39)	missense	probably damaging	1.00
R4214:Notch3	UTSW	17	32,351,181 (GRCm39)	missense	possibly damaging	0.96
R4234:Notch3	UTSW	17	32,360,315 (GRCm39)	missense	probably damaging	0.97
R4242:Notch3	UTSW	17	32,362,719 (GRCm39)	missense	possibly damaging	0.74
R4658:Notch3	UTSW	17	32,373,737 (GRCm39)	missense	probably damaging	1.00
R4878:Notch3	UTSW	17	32,366,059 (GRCm39)	missense	probably damaging	1.00
R4879:Notch3	UTSW	17	32,366,937 (GRCm39)	missense	probably benign	0.00
R4885:Notch3	UTSW	17	32,360,351 (GRCm39)	missense	probably damaging	0.98
R4924:Notch3	UTSW	17	32,363,705 (GRCm39)	missense	probably damaging	1.00
R5084:Notch3	UTSW	17	32,376,864 (GRCm39)	critical splice donor site	probably null
R5086:Notch3	UTSW	17	32,362,308 (GRCm39)	missense	probably benign	0.13
R5343:Notch3	UTSW	17	32,362,257 (GRCm39)	missense	probably benign	0.03
R5389:Notch3	UTSW	17	32,358,163 (GRCm39)	missense	probably benign
R5503:Notch3	UTSW	17	32,366,029 (GRCm39)	missense	probably benign	0.00
R5698:Notch3	UTSW	17	32,376,961 (GRCm39)	missense	probably damaging	1.00
R5824:Notch3	UTSW	17	32,372,835 (GRCm39)	missense	possibly damaging	0.92
R5969:Notch3	UTSW	17	32,372,858 (GRCm39)	missense	probably damaging	1.00
R6050:Notch3	UTSW	17	32,362,501 (GRCm39)	missense	probably benign
R6274:Notch3	UTSW	17	32,366,264 (GRCm39)	missense	probably benign
R6276:Notch3	UTSW	17	32,373,723 (GRCm39)	missense	probably benign	0.10
R6313:Notch3	UTSW	17	32,370,128 (GRCm39)	splice site	probably null
R6316:Notch3	UTSW	17	32,356,787 (GRCm39)	splice site	probably null
R6380:Notch3	UTSW	17	32,363,533 (GRCm39)	missense	probably damaging	1.00
R6401:Notch3	UTSW	17	32,377,597 (GRCm39)	missense	probably benign	0.01
R6502:Notch3	UTSW	17	32,377,191 (GRCm39)	missense	probably damaging	1.00
R6741:Notch3	UTSW	17	32,362,458 (GRCm39)	missense	probably benign	0.16
R7131:Notch3	UTSW	17	32,363,191 (GRCm39)	missense	probably benign
R7140:Notch3	UTSW	17	32,375,351 (GRCm39)	missense	possibly damaging	0.84
R7162:Notch3	UTSW	17	32,365,423 (GRCm39)	missense	probably damaging	0.98
R7171:Notch3	UTSW	17	32,377,936 (GRCm39)	missense	probably damaging	1.00
R7449:Notch3	UTSW	17	32,376,940 (GRCm39)	missense	probably damaging	1.00
R7450:Notch3	UTSW	17	32,360,365 (GRCm39)	missense	possibly damaging	0.69
R7554:Notch3	UTSW	17	32,341,345 (GRCm39)	missense	probably benign	0.03
R7575:Notch3	UTSW	17	32,373,793 (GRCm39)	missense	possibly damaging	0.81
R7632:Notch3	UTSW	17	32,377,480 (GRCm39)	missense	probably benign
R7633:Notch3	UTSW	17	32,377,596 (GRCm39)	missense	probably benign	0.17
R7860:Notch3	UTSW	17	32,341,747 (GRCm39)	missense	possibly damaging	0.67
R8052:Notch3	UTSW	17	32,365,545 (GRCm39)	missense	probably damaging	1.00
R8250:Notch3	UTSW	17	32,351,310 (GRCm39)	missense	probably damaging	1.00
R8296:Notch3	UTSW	17	32,341,713 (GRCm39)	missense	probably damaging	1.00
R8306:Notch3	UTSW	17	32,377,086 (GRCm39)	missense	probably damaging	0.99
R8458:Notch3	UTSW	17	32,375,024 (GRCm39)	missense	probably damaging	1.00
R8539:Notch3	UTSW	17	32,375,329 (GRCm39)	missense	possibly damaging	0.92
R8925:Notch3	UTSW	17	32,372,792 (GRCm39)	missense	probably benign	0.14
R8927:Notch3	UTSW	17	32,372,792 (GRCm39)	missense	probably benign	0.14
R9062:Notch3	UTSW	17	32,341,692 (GRCm39)	missense	possibly damaging	0.93
R9079:Notch3	UTSW	17	32,383,033 (GRCm39)	intron	probably benign
R9089:Notch3	UTSW	17	32,370,521 (GRCm39)	missense	probably benign	0.00
R9260:Notch3	UTSW	17	32,362,216 (GRCm39)	critical splice donor site	probably null
R9289:Notch3	UTSW	17	32,377,254 (GRCm39)	missense	probably damaging	1.00
R9294:Notch3	UTSW	17	32,362,665 (GRCm39)	missense	probably benign	0.03
R9661:Notch3	UTSW	17	32,373,792 (GRCm39)	missense	probably damaging	1.00
R9779:Notch3	UTSW	17	32,372,757 (GRCm39)	missense	probably damaging	1.00
T0975:Notch3	UTSW	17	32,365,391 (GRCm39)	missense	probably damaging	0.99
Z1088:Notch3	UTSW	17	32,377,626 (GRCm39)	missense	possibly damaging	0.94
Z1176:Notch3	UTSW	17	32,370,344 (GRCm39)	missense	probably damaging	1.00
Z1176:Notch3	UTSW	17	32,360,490 (GRCm39)	missense	probably benign	0.12
Z1177:Notch3	UTSW	17	32,385,668 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TAACTTCTGGTTGGGGTCCC -3'
(R):5'- GGTCTGCTCAATCCTGTAGC -3'

Sequencing Primer
(F):5'- CTCCAGAGGCTGTGGCATTG -3'
(R):5'- GTAGCTGTTCCCCTCGACTGG -3'

Posted On

2021-07-15