Mutagenetix > Incidental Mutation

Incidental Mutation 'R8867:Fbxo31'

676017

Institutional Source

Beutler Lab

Gene Symbol

Fbxo31

Ensembl Gene

ENSMUSG00000052934

Gene Name

F-box protein 31

Synonyms

Fbxo14, 2310046N15Rik, Fbx14, 1110003O08Rik

MMRRC Submission

068682-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8867 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

122276179-122305545 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 122281967 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 311 (R311H)

Ref Sequence

ENSEMBL: ENSMUSP00000057573 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059018] [ENSMUST00000127664]

AlphaFold

Q3TQF0

Predicted Effect

probably benign
Transcript: ENSMUST00000059018
AA Change: R311H

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000057573
Gene: ENSMUSG00000052934
AA Change: R311H

Domain	Start	End	E-Value	Type
low complexity region	14	24	N/A	INTRINSIC
low complexity region	25	42	N/A	INTRINSIC
FBOX	56	96	3.45e-8	SMART
low complexity region	358	379	N/A	INTRINSIC
low complexity region	385	412	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000127664

SMART Domains

Protein: ENSMUSP00000118564
Gene: ENSMUSG00000092329

Domain	Start	End	E-Value	Type
Pfam:Glycos_transf_2	104	287	7.4e-31	PFAM
Pfam:Glyco_transf_7C	261	331	4.9e-8	PFAM
RICIN	406	531	9.28e-27	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000212985

Meta Mutation Damage Score

0.1235

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

99% (78/79)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the F-box family. Members are classified into three classes according to the substrate interaction domain, FBW for WD40 repeats, FBL for leucing-rich repeats, and FBXO for other domains. This protein, classified into the last category because of the lack of a recognizable substrate binding domain, has been proposed to be a component of the SCF ubiquitination complex. It is thought to bind and recruit substrate for ubiquitination and degradation. This protein may have a role in regulating the cell cycle as well as dendrite growth and neuronal migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310002L09Rik	G	A	4: 73,861,087 (GRCm39)	S171F	probably damaging	Het
A130010J15Rik	T	C	1: 192,857,406 (GRCm39)	S253P	probably damaging	Het
Acbd5	T	C	2: 22,970,370 (GRCm39)	M97T	possibly damaging	Het
Aire	T	C	10: 77,873,809 (GRCm39)	E300G	probably damaging	Het
Ano1	A	G	7: 144,223,397 (GRCm39)	S99P	possibly damaging	Het
Ap5z1	A	G	5: 142,463,011 (GRCm39)	N732D	probably benign	Het
Apcs	A	T	1: 172,722,004 (GRCm39)	L114Q	possibly damaging	Het
Apmap	T	C	2: 150,431,886 (GRCm39)		probably benign	Het
Ark2c	T	C	18: 77,563,182 (GRCm39)	E159G	possibly damaging	Het
Asxl3	C	A	18: 22,649,547 (GRCm39)	T512N	possibly damaging	Het
Atp1a4	T	C	1: 172,072,491 (GRCm39)	T388A	probably damaging	Het
B3galt9	A	G	2: 34,728,636 (GRCm39)	D145G	probably damaging	Het
Bcl2a1a	A	T	9: 88,839,503 (GRCm39)	I134L	probably damaging	Het
Chd6	G	A	2: 160,862,989 (GRCm39)	A444V	probably benign	Het
Cmbl	C	A	15: 31,582,073 (GRCm39)	H23Q	probably benign	Het
Cnnm2	T	A	19: 46,750,996 (GRCm39)	I262N	probably damaging	Het
Col6a3	C	T	1: 90,715,673 (GRCm39)	R2260H	unknown	Het
Cracr2a	T	A	6: 127,606,736 (GRCm39)	L260*	probably null	Het
Csmd2	A	G	4: 128,451,469 (GRCm39)	Y3409C		Het
Cyp4b1	C	T	4: 115,493,169 (GRCm39)	R225H	possibly damaging	Het
Dcdc2a	C	T	13: 25,386,266 (GRCm39)	A380V	probably benign	Het
Dnah6	A	G	6: 72,998,131 (GRCm39)	L4097P	probably damaging	Het
Dppa3	G	A	6: 122,605,602 (GRCm39)	R52Q	probably benign	Het
Fhad1	T	C	4: 141,656,885 (GRCm39)	R90G	probably damaging	Het
Fitm2	A	T	2: 163,311,602 (GRCm39)	W204R	possibly damaging	Het
Gatb	T	A	3: 85,511,716 (GRCm39)	L157H	probably damaging	Het
Gm5111	G	A	6: 48,566,629 (GRCm39)		probably null	Het
Gm7298	A	C	6: 121,748,788 (GRCm39)	I688L	probably benign	Het
Hecw1	T	C	13: 14,422,275 (GRCm39)		probably null	Het
Ift122	T	A	6: 115,857,632 (GRCm39)	V126E	probably damaging	Het
Iqcf6	A	T	9: 106,504,698 (GRCm39)	I121F	possibly damaging	Het
Kif21a	A	G	15: 90,852,382 (GRCm39)	V902A	probably damaging	Het
Klhl38	T	C	15: 58,178,435 (GRCm39)	M512V	probably benign	Het
Klk1b1	A	C	7: 43,619,747 (GRCm39)	N102T	probably damaging	Het
Lama4	T	A	10: 38,923,996 (GRCm39)	L468Q	probably damaging	Het
Lilra5	A	T	7: 4,241,165 (GRCm39)	Q34L	possibly damaging	Het
Lrrc7	T	A	3: 157,867,521 (GRCm39)	D740V	probably damaging	Het
Mapk7	G	T	11: 61,384,632 (GRCm39)	P25T	probably benign	Het
Mcm3ap	C	A	10: 76,306,538 (GRCm39)	A217D	probably benign	Het
Mucl3	A	T	17: 35,948,872 (GRCm39)	F242L	probably benign	Het
Ncaph2	A	G	15: 89,254,605 (GRCm39)	E406G	probably benign	Het
Ncor2	T	C	5: 125,179,739 (GRCm39)	I69V	unknown	Het
Nfatc3	A	G	8: 106,805,640 (GRCm39)	S170G	probably damaging	Het
Nptn	G	A	9: 58,526,264 (GRCm39)	R137Q	probably damaging	Het
Nsd1	A	T	13: 55,430,570 (GRCm39)	K1581N	probably damaging	Het
Or1q1	T	C	2: 36,887,691 (GRCm39)	Y290H	probably damaging	Het
Or2t43	A	G	11: 58,457,562 (GRCm39)	L203P	probably damaging	Het
Or51l4	G	A	7: 103,403,893 (GRCm39)	Q300*	probably null	Het
Or52ab2	T	A	7: 102,970,449 (GRCm39)	L277Q		Het
Or5be3	G	T	2: 86,863,803 (GRCm39)	T254K	probably damaging	Het
Or6c211	A	T	10: 129,505,628 (GRCm39)	C253*	probably null	Het
Pdcl	T	C	2: 37,242,348 (GRCm39)	E134G	probably damaging	Het
Pikfyve	T	A	1: 65,283,576 (GRCm39)	Y738N	probably damaging	Het
Pkd1	A	G	17: 24,792,807 (GRCm39)	D1498G	probably damaging	Het
Plxna1	T	C	6: 89,310,079 (GRCm39)	N1029D	probably benign	Het
Ppp3cb	C	A	14: 20,596,517 (GRCm39)		probably benign	Het
Prkn	A	G	17: 11,456,448 (GRCm39)	T91A	probably benign	Het
Rgs18	T	A	1: 144,629,698 (GRCm39)	D187V	probably damaging	Het
Ripor2	C	T	13: 24,822,760 (GRCm39)		probably benign	Het
Rpl3l	G	A	17: 24,954,455 (GRCm39)	G172D	probably damaging	Het
Sdf4	A	G	4: 156,093,759 (GRCm39)	T298A	probably damaging	Het
Skic3	C	T	13: 76,279,428 (GRCm39)	S620L	probably damaging	Het
Slc17a3	T	C	13: 24,039,943 (GRCm39)	V327A		Het
Slc4a1ap	T	A	5: 31,708,059 (GRCm39)	F677L	probably benign	Het
Stoml2	A	G	4: 43,028,256 (GRCm39)	V324A	probably benign	Het
Syne2	A	G	12: 75,989,620 (GRCm39)	Q1833R	probably damaging	Het
Syt6	A	G	3: 103,534,371 (GRCm39)	N481D	possibly damaging	Het
Tert	T	C	13: 73,776,566 (GRCm39)	M439T	probably benign	Het
Thsd7a	A	T	6: 12,338,686 (GRCm39)	D1181E		Het
Tln2	G	A	9: 67,237,832 (GRCm39)	T33I	probably damaging	Het
Top3a	A	G	11: 60,633,481 (GRCm39)	S872P	probably benign	Het
Trim10	T	C	17: 37,181,048 (GRCm39)	L93P	probably benign	Het
Trpc7	C	T	13: 57,008,746 (GRCm39)	S307N	probably benign	Het
Ttn	A	T	2: 76,640,427 (GRCm39)	V13727E	probably damaging	Het
Vmn1r220	T	C	13: 23,368,271 (GRCm39)	S142G	probably benign	Het
Vmn1r32	A	G	6: 66,530,651 (GRCm39)	S42P	probably damaging	Het
Zc3h7b	G	A	15: 81,656,681 (GRCm39)	R166Q	probably benign	Het
Zfp683	C	T	4: 133,785,995 (GRCm39)	T374I	probably damaging	Het

Other mutations in Fbxo31

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00983:Fbxo31	APN	8	122,281,069 (GRCm39)	missense	possibly damaging	0.92
IGL02155:Fbxo31	APN	8	122,285,814 (GRCm39)	missense	probably damaging	1.00
IGL02551:Fbxo31	APN	8	122,293,083 (GRCm39)	missense	probably damaging	0.99
IGL03092:Fbxo31	APN	8	122,286,757 (GRCm39)	missense	probably benign
Archive	UTSW	8	122,281,967 (GRCm39)	missense	probably benign
Repository	UTSW	8	122,281,136 (GRCm39)	missense	probably damaging	0.98
R0377:Fbxo31	UTSW	8	122,285,841 (GRCm39)	unclassified	probably benign
R0730:Fbxo31	UTSW	8	122,282,103 (GRCm39)	unclassified	probably benign
R1132:Fbxo31	UTSW	8	122,279,019 (GRCm39)	frame shift	probably null
R1132:Fbxo31	UTSW	8	122,279,015 (GRCm39)	frame shift	probably null
R1626:Fbxo31	UTSW	8	122,286,745 (GRCm39)	missense	probably damaging	1.00
R1796:Fbxo31	UTSW	8	122,287,177 (GRCm39)	nonsense	probably null
R2215:Fbxo31	UTSW	8	122,293,050 (GRCm39)	missense	probably benign	0.01
R3726:Fbxo31	UTSW	8	122,305,248 (GRCm39)	missense	probably damaging	1.00
R3761:Fbxo31	UTSW	8	122,287,169 (GRCm39)	missense	possibly damaging	0.94
R4646:Fbxo31	UTSW	8	122,286,755 (GRCm39)	missense	probably benign
R4782:Fbxo31	UTSW	8	122,279,180 (GRCm39)	missense	probably damaging	1.00
R4782:Fbxo31	UTSW	8	122,279,178 (GRCm39)	nonsense	probably null
R5103:Fbxo31	UTSW	8	122,279,101 (GRCm39)	missense	probably damaging	1.00
R5715:Fbxo31	UTSW	8	122,305,302 (GRCm39)	missense	probably damaging	1.00
R6347:Fbxo31	UTSW	8	122,305,198 (GRCm39)	missense	possibly damaging	0.69
R6551:Fbxo31	UTSW	8	122,291,443 (GRCm39)	intron	probably benign
R7027:Fbxo31	UTSW	8	122,305,224 (GRCm39)	missense	probably damaging	1.00
R7156:Fbxo31	UTSW	8	122,281,060 (GRCm39)	missense	possibly damaging	0.83
R7271:Fbxo31	UTSW	8	122,305,503 (GRCm39)	unclassified	probably benign
R7594:Fbxo31	UTSW	8	122,279,107 (GRCm39)	missense	probably damaging	1.00
R7860:Fbxo31	UTSW	8	122,291,384 (GRCm39)	splice site	probably null
R8039:Fbxo31	UTSW	8	122,285,794 (GRCm39)	missense	probably damaging	1.00
R8116:Fbxo31	UTSW	8	122,287,127 (GRCm39)	missense	probably damaging	1.00
R8284:Fbxo31	UTSW	8	122,287,181 (GRCm39)	missense	probably benign	0.01
R8726:Fbxo31	UTSW	8	122,282,014 (GRCm39)	nonsense	probably null
R9081:Fbxo31	UTSW	8	122,281,136 (GRCm39)	missense	probably damaging	0.98
R9082:Fbxo31	UTSW	8	122,281,136 (GRCm39)	missense	probably damaging	0.98
R9093:Fbxo31	UTSW	8	122,281,136 (GRCm39)	missense	probably damaging	0.98
R9094:Fbxo31	UTSW	8	122,281,136 (GRCm39)	missense	probably damaging	0.98
R9095:Fbxo31	UTSW	8	122,281,136 (GRCm39)	missense	probably damaging	0.98
R9098:Fbxo31	UTSW	8	122,281,136 (GRCm39)	missense	probably damaging	0.98
R9667:Fbxo31	UTSW	8	122,305,208 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CCACACCAACATGAAGTGTG -3'
(R):5'- CAAGGTCTAGGGCAAATGGC -3'

Sequencing Primer
(F):5'- AAATCTGGGATACAGGGC -3'
(R):5'- ATAGAGGATGCCTGCCTAGC -3'

Posted On

2021-07-15