Mutagenetix > Incidental Mutation

Incidental Mutation 'R8868:Zfp260'

676084

Institutional Source

Beutler Lab

Gene Symbol

Zfp260

Ensembl Gene

ENSMUSG00000049421

Gene Name

zinc finger protein 260

Synonyms

PEX1, Zfp63, Ozrf1

MMRRC Submission

068683-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.103) question?

Stock #

R8868 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

29794207-29807047 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to G at 29804914 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 271 (Y271*)

Ref Sequence

ENSEMBL: ENSMUSP00000052200 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000050735] [ENSMUST00000108200] [ENSMUST00000130526]

AlphaFold

Q62513

Predicted Effect

probably null
Transcript: ENSMUST00000050735
AA Change: Y271*

SMART Domains

Protein: ENSMUSP00000052200
Gene: ENSMUSG00000049421
AA Change: Y271*

Domain	Start	End	E-Value	Type
ZnF_C2H2	23	45	6.78e-3	SMART
ZnF_C2H2	51	71	2.44e2	SMART
ZnF_C2H2	79	101	3.21e-4	SMART
ZnF_C2H2	131	153	5.59e-4	SMART
ZnF_C2H2	159	181	1.38e-3	SMART
ZnF_C2H2	187	209	9.88e-5	SMART
ZnF_C2H2	215	237	8.47e-4	SMART
ZnF_C2H2	243	265	1.6e-4	SMART
ZnF_C2H2	271	293	8.94e-3	SMART
ZnF_C2H2	299	321	3.21e-4	SMART
ZnF_C2H2	327	349	9.88e-5	SMART
ZnF_C2H2	355	377	1.38e-3	SMART
ZnF_C2H2	383	405	1.38e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000108200
AA Change: Y271*

SMART Domains

Protein: ENSMUSP00000103835
Gene: ENSMUSG00000049421
AA Change: Y271*

Domain	Start	End	E-Value	Type
ZnF_C2H2	23	45	6.78e-3	SMART
ZnF_C2H2	51	71	2.44e2	SMART
ZnF_C2H2	79	101	3.21e-4	SMART
ZnF_C2H2	131	153	5.59e-4	SMART
ZnF_C2H2	159	181	1.38e-3	SMART
ZnF_C2H2	187	209	9.88e-5	SMART
ZnF_C2H2	215	237	8.47e-4	SMART
ZnF_C2H2	243	265	1.6e-4	SMART
ZnF_C2H2	271	293	8.94e-3	SMART
ZnF_C2H2	299	321	3.21e-4	SMART
ZnF_C2H2	327	349	9.88e-5	SMART
ZnF_C2H2	355	377	1.38e-3	SMART
ZnF_C2H2	383	405	1.38e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000130526

Meta Mutation Damage Score

0.9756

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

100% (64/64)

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acad8	T	A	9: 26,890,544 (GRCm39)	N255Y	probably damaging	Het
Accsl	A	C	2: 93,696,490 (GRCm39)	L36R	probably benign	Het
Acvr2a	T	A	2: 48,763,469 (GRCm39)	V171E	probably benign	Het
Adgrl3	A	G	5: 81,794,451 (GRCm39)	I491V	probably benign	Het
Ak9	G	A	10: 41,193,842 (GRCm39)		probably null	Het
Ak9	G	T	10: 41,258,869 (GRCm39)	E789*	probably null	Het
Akr1c19	T	A	13: 4,293,070 (GRCm39)	N275K	probably benign	Het
Alg1	A	G	16: 5,061,557 (GRCm39)	H398R	probably benign	Het
Apol11a	T	A	15: 77,401,209 (GRCm39)	I232N	probably damaging	Het
Cacnb2	A	G	2: 14,989,080 (GRCm39)	I437V	probably benign	Het
Catsper4	A	G	4: 133,954,417 (GRCm39)		probably null	Het
Cdhr17	G	A	5: 17,028,152 (GRCm39)	G449D	probably benign	Het
Crnn	T	C	3: 93,055,609 (GRCm39)	C132R	probably benign	Het
Cyp4a14	T	A	4: 115,348,553 (GRCm39)	H337L	probably damaging	Het
Dcdc2a	C	T	13: 25,386,266 (GRCm39)	A380V	probably benign	Het
Dclre1b	T	C	3: 103,710,654 (GRCm39)	N419S	probably benign	Het
Dip2c	A	G	13: 9,625,503 (GRCm39)	D621G	possibly damaging	Het
Dmwd	T	A	7: 18,814,694 (GRCm39)	L448Q	probably damaging	Het
Dnajc13	G	A	9: 104,042,987 (GRCm39)	H1922Y	probably benign	Het
E2f3	A	T	13: 30,095,301 (GRCm39)	I329N	probably damaging	Het
Eml2	G	A	7: 18,927,988 (GRCm39)	V266M	probably benign	Het
Entpd2	A	G	2: 25,289,725 (GRCm39)	T376A	probably benign	Het
Flnb	A	G	14: 7,908,671 (GRCm38)	T1350A	probably benign	Het
Fmnl2	A	G	2: 53,016,077 (GRCm39)	E999G	unknown	Het
Hivep1	A	G	13: 42,312,407 (GRCm39)	Q1549R	possibly damaging	Het
Hps6	G	A	19: 45,992,446 (GRCm39)	V128M	possibly damaging	Het
Ifi27l2a	T	C	12: 103,402,899 (GRCm39)	S66P	possibly damaging	Het
Ikbip	T	C	10: 90,932,187 (GRCm39)	V277A	possibly damaging	Het
Ints8	T	C	4: 11,230,488 (GRCm39)	E451G	probably benign	Het
Itih2	A	T	2: 10,132,600 (GRCm39)	S64T	probably benign	Het
Kcnc4	T	A	3: 107,355,452 (GRCm39)	E332V	probably damaging	Het
Krt23	T	C	11: 99,374,567 (GRCm39)		probably benign	Het
Lrpprc	C	A	17: 85,078,920 (GRCm39)	G265C	probably damaging	Het
Nav1	G	T	1: 135,512,943 (GRCm39)	A39E	probably benign	Het
Ogfod1	G	T	8: 94,773,906 (GRCm39)	D103Y	probably damaging	Het
Or7g20	T	G	9: 18,946,778 (GRCm39)	Y120D	probably damaging	Het
Or8b55	G	A	9: 38,727,285 (GRCm39)	C162Y	probably damaging	Het
Or8g37	A	G	9: 39,731,252 (GRCm39)	T106A	probably benign	Het
Ppp1r3a	A	T	6: 14,755,014 (GRCm39)	S78T	probably damaging	Het
Rnf148	A	T	6: 23,654,540 (GRCm39)	V152E	probably damaging	Het
Sdf2	A	T	11: 78,136,970 (GRCm39)	H50L	probably damaging	Het
Sec1	T	A	7: 45,328,700 (GRCm39)	M116L	probably benign	Het
Sema6d	T	C	2: 124,496,114 (GRCm39)	S53P	probably damaging	Het
Siae	A	G	9: 37,528,132 (GRCm39)	Y35C	probably damaging	Het
Slc18b1	A	T	10: 23,686,751 (GRCm39)	Y189F	probably damaging	Het
Slc35d1	T	C	4: 103,065,351 (GRCm39)	T177A	probably damaging	Het
Spef2	T	C	15: 9,729,747 (GRCm39)	T124A	possibly damaging	Het
Strn4	G	A	7: 16,560,570 (GRCm39)	E241K	probably benign	Het
Svep1	A	G	4: 58,135,578 (GRCm39)	I510T	possibly damaging	Het
Svop	T	C	5: 114,170,854 (GRCm39)	D395G	probably damaging	Het
Tbc1d14	A	C	5: 36,728,888 (GRCm39)	S159R	probably damaging	Het
Tex50	T	A	1: 160,984,848 (GRCm39)	I131F	possibly damaging	Het
Tssk1	A	G	16: 17,712,884 (GRCm39)	K223R	probably benign	Het
Ttc3	A	G	16: 94,252,002 (GRCm39)	I1565V	probably benign	Het
Ttn	A	T	2: 76,541,938 (GRCm39)	F33683I	possibly damaging	Het
Ttn	T	C	2: 76,783,470 (GRCm39)	M887V	unknown	Het
Usp45	G	A	4: 21,815,399 (GRCm39)		probably null	Het
Wdr47	C	T	3: 108,498,841 (GRCm39)		probably benign	Het
Xirp1	A	G	9: 119,846,871 (GRCm39)	S671P	probably benign	Het
Zbtb18	A	G	1: 177,274,682 (GRCm39)	D5G	probably benign	Het
Zc3h7b	G	A	15: 81,656,681 (GRCm39)	R166Q	probably benign	Het
Zfp369	T	A	13: 65,445,082 (GRCm39)	S742T	possibly damaging	Het
Zfp786	A	T	6: 47,802,015 (GRCm39)	D87E	probably damaging	Het

Other mutations in Zfp260

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1739:Zfp260	UTSW	7	29,804,231 (GRCm39)	missense	probably benign	0.04
R2143:Zfp260	UTSW	7	29,804,765 (GRCm39)	missense	probably damaging	1.00
R2144:Zfp260	UTSW	7	29,804,765 (GRCm39)	missense	probably damaging	1.00
R2145:Zfp260	UTSW	7	29,804,765 (GRCm39)	missense	probably damaging	1.00
R2400:Zfp260	UTSW	7	29,804,126 (GRCm39)	missense	possibly damaging	0.83
R3802:Zfp260	UTSW	7	29,804,504 (GRCm39)	missense	probably benign	0.01
R5250:Zfp260	UTSW	7	29,804,392 (GRCm39)	missense	probably damaging	1.00
R6313:Zfp260	UTSW	7	29,804,267 (GRCm39)	missense	possibly damaging	0.86
R6416:Zfp260	UTSW	7	29,804,235 (GRCm39)	missense	possibly damaging	0.58
R7369:Zfp260	UTSW	7	29,804,750 (GRCm39)	missense	probably damaging	1.00
R7507:Zfp260	UTSW	7	29,804,291 (GRCm39)	missense	probably damaging	0.99
R7920:Zfp260	UTSW	7	29,805,017 (GRCm39)	nonsense	probably null
R8531:Zfp260	UTSW	7	29,804,884 (GRCm39)	missense	probably damaging	1.00
R9304:Zfp260	UTSW	7	29,804,279 (GRCm39)	missense	possibly damaging	0.55
R9372:Zfp260	UTSW	7	29,804,232 (GRCm39)	missense	probably benign	0.15
R9443:Zfp260	UTSW	7	29,804,249 (GRCm39)	missense	probably benign	0.00
R9579:Zfp260	UTSW	7	29,805,108 (GRCm39)	missense
R9801:Zfp260	UTSW	7	29,804,937 (GRCm39)	missense	possibly damaging	0.49
Z1186:Zfp260	UTSW	7	29,804,463 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TCCCATGACAACCACCGTTAGAA -3'
(R):5'- TGGGACTTCTGGCTGAAAGC -3'

Sequencing Primer
(F):5'- TCCATACTGGAGAGAAGCCTTATG -3'
(R):5'- GGCTCCTCATATGCACTGTAAGAG -3'

Posted On

2021-07-15