Mutagenetix > Incidental Mutation

Incidental Mutation 'R8868:Zfp369'

676105

Institutional Source

Beutler Lab

Gene Symbol

Zfp369

Ensembl Gene

ENSMUSG00000021514

Gene Name

zinc finger protein 369

Synonyms

B930030B22Rik, NRIF2, D230020H11Rik

MMRRC Submission

068683-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R8868 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

65426628-65452035 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 65445082 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 742 (S742T)

Ref Sequence

ENSEMBL: ENSMUSP00000119114 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000126879] [ENSMUST00000130799]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000126879
AA Change: S742T

PolyPhen 2 Score 0.603 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000119114
Gene: ENSMUSG00000021514
AA Change: S742T

Domain	Start	End	E-Value	Type
KRAB	35	95	7.13e-20	SMART
SCAN	178	289	2.57e-39	SMART
KRAB	300	367	1.63e-14	SMART
low complexity region	440	452	N/A	INTRINSIC
ZnF_C2H2	701	723	5.42e-2	SMART
ZnF_C2H2	729	751	4.79e-3	SMART
ZnF_C2H2	757	779	1.47e-3	SMART
ZnF_C2H2	785	807	2.43e-4	SMART
ZnF_C2H2	813	835	6.88e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000130799

SMART Domains

Protein: ENSMUSP00000116221
Gene: ENSMUSG00000021514

Domain	Start	End	E-Value	Type
KRAB	35	95	7.13e-20	SMART
SCAN	178	289	2.57e-39	SMART
KRAB	300	354	3.54e-15	SMART

Meta Mutation Damage Score

0.6500

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

100% (64/64)

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acad8	T	A	9: 26,890,544 (GRCm39)	N255Y	probably damaging	Het
Accsl	A	C	2: 93,696,490 (GRCm39)	L36R	probably benign	Het
Acvr2a	T	A	2: 48,763,469 (GRCm39)	V171E	probably benign	Het
Adgrl3	A	G	5: 81,794,451 (GRCm39)	I491V	probably benign	Het
Ak9	G	A	10: 41,193,842 (GRCm39)		probably null	Het
Ak9	G	T	10: 41,258,869 (GRCm39)	E789*	probably null	Het
Akr1c19	T	A	13: 4,293,070 (GRCm39)	N275K	probably benign	Het
Alg1	A	G	16: 5,061,557 (GRCm39)	H398R	probably benign	Het
Apol11a	T	A	15: 77,401,209 (GRCm39)	I232N	probably damaging	Het
Cacnb2	A	G	2: 14,989,080 (GRCm39)	I437V	probably benign	Het
Catsper4	A	G	4: 133,954,417 (GRCm39)		probably null	Het
Cdhr17	G	A	5: 17,028,152 (GRCm39)	G449D	probably benign	Het
Crnn	T	C	3: 93,055,609 (GRCm39)	C132R	probably benign	Het
Cyp4a14	T	A	4: 115,348,553 (GRCm39)	H337L	probably damaging	Het
Dcdc2a	C	T	13: 25,386,266 (GRCm39)	A380V	probably benign	Het
Dclre1b	T	C	3: 103,710,654 (GRCm39)	N419S	probably benign	Het
Dip2c	A	G	13: 9,625,503 (GRCm39)	D621G	possibly damaging	Het
Dmwd	T	A	7: 18,814,694 (GRCm39)	L448Q	probably damaging	Het
Dnajc13	G	A	9: 104,042,987 (GRCm39)	H1922Y	probably benign	Het
E2f3	A	T	13: 30,095,301 (GRCm39)	I329N	probably damaging	Het
Eml2	G	A	7: 18,927,988 (GRCm39)	V266M	probably benign	Het
Entpd2	A	G	2: 25,289,725 (GRCm39)	T376A	probably benign	Het
Flnb	A	G	14: 7,908,671 (GRCm38)	T1350A	probably benign	Het
Fmnl2	A	G	2: 53,016,077 (GRCm39)	E999G	unknown	Het
Hivep1	A	G	13: 42,312,407 (GRCm39)	Q1549R	possibly damaging	Het
Hps6	G	A	19: 45,992,446 (GRCm39)	V128M	possibly damaging	Het
Ifi27l2a	T	C	12: 103,402,899 (GRCm39)	S66P	possibly damaging	Het
Ikbip	T	C	10: 90,932,187 (GRCm39)	V277A	possibly damaging	Het
Ints8	T	C	4: 11,230,488 (GRCm39)	E451G	probably benign	Het
Itih2	A	T	2: 10,132,600 (GRCm39)	S64T	probably benign	Het
Kcnc4	T	A	3: 107,355,452 (GRCm39)	E332V	probably damaging	Het
Krt23	T	C	11: 99,374,567 (GRCm39)		probably benign	Het
Lrpprc	C	A	17: 85,078,920 (GRCm39)	G265C	probably damaging	Het
Nav1	G	T	1: 135,512,943 (GRCm39)	A39E	probably benign	Het
Ogfod1	G	T	8: 94,773,906 (GRCm39)	D103Y	probably damaging	Het
Or7g20	T	G	9: 18,946,778 (GRCm39)	Y120D	probably damaging	Het
Or8b55	G	A	9: 38,727,285 (GRCm39)	C162Y	probably damaging	Het
Or8g37	A	G	9: 39,731,252 (GRCm39)	T106A	probably benign	Het
Ppp1r3a	A	T	6: 14,755,014 (GRCm39)	S78T	probably damaging	Het
Rnf148	A	T	6: 23,654,540 (GRCm39)	V152E	probably damaging	Het
Sdf2	A	T	11: 78,136,970 (GRCm39)	H50L	probably damaging	Het
Sec1	T	A	7: 45,328,700 (GRCm39)	M116L	probably benign	Het
Sema6d	T	C	2: 124,496,114 (GRCm39)	S53P	probably damaging	Het
Siae	A	G	9: 37,528,132 (GRCm39)	Y35C	probably damaging	Het
Slc18b1	A	T	10: 23,686,751 (GRCm39)	Y189F	probably damaging	Het
Slc35d1	T	C	4: 103,065,351 (GRCm39)	T177A	probably damaging	Het
Spef2	T	C	15: 9,729,747 (GRCm39)	T124A	possibly damaging	Het
Strn4	G	A	7: 16,560,570 (GRCm39)	E241K	probably benign	Het
Svep1	A	G	4: 58,135,578 (GRCm39)	I510T	possibly damaging	Het
Svop	T	C	5: 114,170,854 (GRCm39)	D395G	probably damaging	Het
Tbc1d14	A	C	5: 36,728,888 (GRCm39)	S159R	probably damaging	Het
Tex50	T	A	1: 160,984,848 (GRCm39)	I131F	possibly damaging	Het
Tssk1	A	G	16: 17,712,884 (GRCm39)	K223R	probably benign	Het
Ttc3	A	G	16: 94,252,002 (GRCm39)	I1565V	probably benign	Het
Ttn	A	T	2: 76,541,938 (GRCm39)	F33683I	possibly damaging	Het
Ttn	T	C	2: 76,783,470 (GRCm39)	M887V	unknown	Het
Usp45	G	A	4: 21,815,399 (GRCm39)		probably null	Het
Wdr47	C	T	3: 108,498,841 (GRCm39)		probably benign	Het
Xirp1	A	G	9: 119,846,871 (GRCm39)	S671P	probably benign	Het
Zbtb18	A	G	1: 177,274,682 (GRCm39)	D5G	probably benign	Het
Zc3h7b	G	A	15: 81,656,681 (GRCm39)	R166Q	probably benign	Het
Zfp260	T	G	7: 29,804,914 (GRCm39)	Y271*	probably null	Het
Zfp786	A	T	6: 47,802,015 (GRCm39)	D87E	probably damaging	Het

Other mutations in Zfp369

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00969:Zfp369	APN	13	65,445,074 (GRCm39)	missense	probably benign	0.41
IGL01393:Zfp369	APN	13	65,442,288 (GRCm39)	missense	possibly damaging	0.85
IGL02277:Zfp369	APN	13	65,432,746 (GRCm39)	missense	probably damaging	1.00
IGL02986:Zfp369	APN	13	65,428,095 (GRCm39)	missense	probably damaging	1.00
IGL02992:Zfp369	APN	13	65,442,265 (GRCm39)	missense	possibly damaging	0.50
IGL03170:Zfp369	APN	13	65,442,224 (GRCm39)	missense	probably damaging	0.99
IGL03351:Zfp369	APN	13	65,443,967 (GRCm39)	missense	possibly damaging	0.95
R0136:Zfp369	UTSW	13	65,445,016 (GRCm39)	missense	probably benign	0.08
R0600:Zfp369	UTSW	13	65,444,248 (GRCm39)	missense	probably damaging	0.97
R0646:Zfp369	UTSW	13	65,445,362 (GRCm39)	missense	probably damaging	1.00
R1190:Zfp369	UTSW	13	65,440,107 (GRCm39)	missense	probably damaging	0.99
R1191:Zfp369	UTSW	13	65,439,776 (GRCm39)	nonsense	probably null
R1528:Zfp369	UTSW	13	65,439,979 (GRCm39)	missense	probably damaging	1.00
R1934:Zfp369	UTSW	13	65,444,965 (GRCm39)	missense	probably damaging	1.00
R2168:Zfp369	UTSW	13	65,444,762 (GRCm39)	missense	probably benign	0.01
R4386:Zfp369	UTSW	13	65,444,806 (GRCm39)	missense	probably benign	0.00
R4928:Zfp369	UTSW	13	65,444,614 (GRCm39)	missense	possibly damaging	0.91
R5127:Zfp369	UTSW	13	65,426,847 (GRCm39)	start gained	probably benign
R5549:Zfp369	UTSW	13	65,445,194 (GRCm39)	missense	probably damaging	1.00
R5740:Zfp369	UTSW	13	65,444,581 (GRCm39)	missense	probably benign
R5743:Zfp369	UTSW	13	65,443,494 (GRCm39)	missense	probably benign	0.02
R5840:Zfp369	UTSW	13	65,445,092 (GRCm39)	missense	possibly damaging	0.46
R6351:Zfp369	UTSW	13	65,444,044 (GRCm39)	missense	possibly damaging	0.83
R7248:Zfp369	UTSW	13	65,443,931 (GRCm39)	missense	probably damaging	0.98
R7288:Zfp369	UTSW	13	65,432,832 (GRCm39)	critical splice donor site	probably null
R7314:Zfp369	UTSW	13	65,439,918 (GRCm39)	missense	probably damaging	0.97
R7470:Zfp369	UTSW	13	65,439,960 (GRCm39)	missense	probably benign	0.12
R7508:Zfp369	UTSW	13	65,427,087 (GRCm39)	missense	unknown
R7796:Zfp369	UTSW	13	65,444,029 (GRCm39)	missense	probably benign
R7886:Zfp369	UTSW	13	65,439,868 (GRCm39)	missense	possibly damaging	0.95
R8425:Zfp369	UTSW	13	65,444,489 (GRCm39)	missense	possibly damaging	0.96
R8671:Zfp369	UTSW	13	65,444,095 (GRCm39)	missense	possibly damaging	0.92
R8918:Zfp369	UTSW	13	65,443,529 (GRCm39)	nonsense	probably null
R8921:Zfp369	UTSW	13	65,444,044 (GRCm39)	missense	possibly damaging	0.92
R9201:Zfp369	UTSW	13	65,444,396 (GRCm39)	missense	probably benign	0.07
R9236:Zfp369	UTSW	13	65,445,010 (GRCm39)	missense	probably benign	0.08
R9443:Zfp369	UTSW	13	65,444,347 (GRCm39)	missense	possibly damaging	0.93
Z1177:Zfp369	UTSW	13	65,439,974 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GGCATGCTCAGCAGATATCC -3'
(R):5'- ACTTGGTACACACATAAGGTCGC -3'

Sequencing Primer
(F):5'- CTTTATAAAAATTCACAAGGGGAGCC -3'
(R):5'- ATAAGGTCGCTCCCCAGTATG -3'

Posted On

2021-07-15