Mutagenetix > Incidental Mutation

Incidental Mutation 'R8877:Msantd5f6'

676625

Institutional Source

Beutler Lab

Gene Symbol

Msantd5f6

Ensembl Gene

ENSMUSG00000066137

Gene Name

Myb/SANT DNA binding domain containing 5 family member 6

Synonyms

Gm11487

MMRRC Submission

068745-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.148) question?

Stock #

R8877 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

73319269-73323309 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 73322468 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 12 (R12*)

Ref Sequence

ENSEMBL: ENSMUSP00000081520 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000084480]

AlphaFold

Q5RIS0

Predicted Effect

probably null
Transcript: ENSMUST00000084480
AA Change: R12*

SMART Domains

Protein: ENSMUSP00000081520
Gene: ENSMUSG00000066137
AA Change: R12*

Domain	Start	End	E-Value	Type
low complexity region	69	84	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000147434

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

100% (58/58)

MGI Phenotype

FUNCTION: This gene belongs to a family of related genes tandemly arranged in two clusters on chromosome 4. This family, which appears to be mouse-specific and composed of multiple highly similar members, is supported by limited transcript data. Members of the family maintain an intact open reading frame although the encoded protein has no known function. This gene is inferred from alignment of paralogous transcripts. [provided by RefSeq, Apr 2013]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2900092C05Rik	T	A	7: 12,288,704 (GRCm39)		probably null	Het
Acp2	G	A	2: 91,036,129 (GRCm39)	R109H	probably damaging	Het
Adamts8	T	C	9: 30,862,688 (GRCm39)	S298P	probably damaging	Het
Anxa7	A	G	14: 20,517,548 (GRCm39)	V157A	probably benign	Het
Arid3b	T	C	9: 57,740,904 (GRCm39)	K181E	probably damaging	Het
Armt1	A	G	10: 4,400,864 (GRCm39)	T204A	possibly damaging	Het
Brip1	A	C	11: 86,043,532 (GRCm39)	V344G	possibly damaging	Het
Ccdc40	C	T	11: 119,153,992 (GRCm39)	S1088L	probably damaging	Het
Ccdc93	A	T	1: 121,403,867 (GRCm39)	H332L	probably benign	Het
Celsr3	A	G	9: 108,706,877 (GRCm39)	D1120G	probably damaging	Het
Cep104	T	A	4: 154,077,985 (GRCm39)	I631N	probably damaging	Het
Col17a1	C	T	19: 47,637,197 (GRCm39)	A1354T	unknown	Het
Cpa2	T	C	6: 30,541,692 (GRCm39)	L10P	probably damaging	Het
Creg2	G	T	1: 39,689,861 (GRCm39)	T83K	probably benign	Het
Ddit3	T	C	10: 127,131,884 (GRCm39)	I144T	probably damaging	Het
Dmxl1	A	G	18: 50,011,292 (GRCm39)	I1150V	possibly damaging	Het
Eprs1	C	T	1: 185,148,071 (GRCm39)	R1278*	probably null	Het
Esco1	A	G	18: 10,575,017 (GRCm39)	V685A	probably damaging	Het
Gid8	C	T	2: 180,358,710 (GRCm39)	A125V	probably damaging	Het
Gin1	A	G	1: 97,710,941 (GRCm39)	D208G	possibly damaging	Het
Gja10	G	A	4: 32,602,441 (GRCm39)		probably benign	Het
Grin2d	T	C	7: 45,503,699 (GRCm39)	I679V	probably damaging	Het
Gsto2	C	A	19: 47,873,176 (GRCm39)	R184S	probably damaging	Het
Hira	T	A	16: 18,770,854 (GRCm39)	H830Q	probably benign	Het
Hmcn1	G	A	1: 150,514,659 (GRCm39)	T3571I	probably benign	Het
Khnyn	T	C	14: 56,131,782 (GRCm39)	V568A	possibly damaging	Het
Kif13a	T	A	13: 46,954,921 (GRCm39)		probably null	Het
Larp4b	T	C	13: 9,193,835 (GRCm39)	V161A	probably benign	Het
Larp6	T	A	9: 60,644,850 (GRCm39)	M330K	probably benign	Het
Lhfpl6	A	G	3: 52,950,974 (GRCm39)	R83G	possibly damaging	Het
Mavs	A	G	2: 131,087,489 (GRCm39)	N329S	possibly damaging	Het
Mep1b	T	A	18: 21,221,630 (GRCm39)	N193K	possibly damaging	Het
Mttp	A	T	3: 137,818,317 (GRCm39)	D380E	probably damaging	Het
Nbeal2	G	A	9: 110,459,311 (GRCm39)	T1939I	probably damaging	Het
Nsun7	A	T	5: 66,453,294 (GRCm39)	R670*	probably null	Het
Obsl1	G	A	1: 75,473,167 (GRCm39)	R1019*	probably null	Het
Or10ak12	A	G	4: 118,666,482 (GRCm39)	V193A	probably damaging	Het
Or5w11	T	C	2: 87,459,212 (GRCm39)	M19T	probably damaging	Het
Phc3	C	T	3: 30,968,271 (GRCm39)	V922I	probably damaging	Het
Pip5kl1	T	C	2: 32,468,951 (GRCm39)	V190A	possibly damaging	Het
Plekhg2	G	T	7: 28,060,278 (GRCm39)	T993N	possibly damaging	Het
Psg26	C	T	7: 18,217,865 (GRCm39)	V18I	probably benign	Het
Slc11a1	A	G	1: 74,419,424 (GRCm39)	Y187C	probably damaging	Het
Smarcd3	A	G	5: 24,798,990 (GRCm39)	S323P	possibly damaging	Het
Spen	G	T	4: 141,199,137 (GRCm39)	Y3163*	probably null	Het
Stx4a	T	A	7: 127,447,633 (GRCm39)	V259D	probably damaging	Het
Synj2	T	C	17: 6,087,941 (GRCm39)	S1331P	probably damaging	Het
Tmprss11c	G	A	5: 86,385,540 (GRCm39)	Q311*	probably null	Het
Tpo	T	A	12: 30,142,738 (GRCm39)	N662I	probably damaging	Het
Trav13d-4	C	A	14: 53,995,350 (GRCm39)	D101E	probably damaging	Het
Trbv14	G	A	6: 41,112,292 (GRCm39)	A30T	probably benign	Het
Unc80	T	C	1: 66,567,144 (GRCm39)	S917P	possibly damaging	Het
Zc3h6	T	A	2: 128,856,319 (GRCm39)	C466*	probably null	Het
Zfp462	T	C	4: 55,011,097 (GRCm39)	V1021A	probably damaging	Het
Zfp51	T	C	17: 21,682,017 (GRCm39)	M72T	probably damaging	Het
Zfp704	C	T	3: 9,674,416 (GRCm39)	E122K	unknown	Het
Zfyve26	T	C	12: 79,334,152 (GRCm39)	K289E	probably benign	Het

Other mutations in Msantd5f6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02373:Msantd5f6	APN	4	73,321,880 (GRCm39)	missense	probably benign	0.01
IGL03275:Msantd5f6	APN	4	73,321,653 (GRCm39)	missense	possibly damaging	0.80
R1066:Msantd5f6	UTSW	4	73,320,066 (GRCm39)	missense	possibly damaging	0.46
R1274:Msantd5f6	UTSW	4	73,321,313 (GRCm39)	missense	probably damaging	0.97
R1742:Msantd5f6	UTSW	4	73,319,447 (GRCm39)	missense	probably damaging	1.00
R1863:Msantd5f6	UTSW	4	73,320,037 (GRCm39)	nonsense	probably null
R1903:Msantd5f6	UTSW	4	73,321,675 (GRCm39)	missense	probably damaging	0.98
R2027:Msantd5f6	UTSW	4	73,321,295 (GRCm39)	missense	possibly damaging	0.46
R4011:Msantd5f6	UTSW	4	73,320,047 (GRCm39)	missense	probably damaging	0.97
R4801:Msantd5f6	UTSW	4	73,319,504 (GRCm39)	nonsense	probably null
R4802:Msantd5f6	UTSW	4	73,319,504 (GRCm39)	nonsense	probably null
R5213:Msantd5f6	UTSW	4	73,319,571 (GRCm39)	missense	probably damaging	0.96
R5334:Msantd5f6	UTSW	4	73,321,754 (GRCm39)	missense	probably benign	0.31
R5345:Msantd5f6	UTSW	4	73,319,514 (GRCm39)	missense	probably damaging	1.00
R6093:Msantd5f6	UTSW	4	73,320,258 (GRCm39)	missense	probably benign	0.05
R6786:Msantd5f6	UTSW	4	73,321,843 (GRCm39)	missense	possibly damaging	0.86
R8033:Msantd5f6	UTSW	4	73,321,329 (GRCm39)	missense	probably benign	0.01
R8093:Msantd5f6	UTSW	4	73,321,759 (GRCm39)	missense	probably damaging	0.98
R8975:Msantd5f6	UTSW	4	73,320,167 (GRCm39)	missense	probably damaging	0.97
R9191:Msantd5f6	UTSW	4	73,319,670 (GRCm39)	missense	probably damaging	0.96
R9230:Msantd5f6	UTSW	4	73,319,685 (GRCm39)	missense	probably benign	0.05
R9338:Msantd5f6	UTSW	4	73,320,245 (GRCm39)	missense	possibly damaging	0.82

Predicted Primers

PCR Primer
(F):5'- AGACACTCAGTGCCTTCTGG -3'
(R):5'- CACAGGCCTACTTTCCCATTAG -3'

Sequencing Primer
(F):5'- CCTTCTGGGACAAGGGAATAAATC -3'
(R):5'- GGCCTACTTTCCCATTAGAAGAG -3'

Posted On

2021-07-15