Mutagenetix > Incidental Mutation

Incidental Mutation 'R8879:Ttc25'

676785

Institutional Source

Beutler Lab

Gene Symbol

Ttc25

Ensembl Gene

ENSMUSG00000006784

Gene Name

tetratricopeptide repeat domain 25

Synonyms

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.147) question?

Stock #

R8879 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

100545607-100572568 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to T at 100566926 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 452 (E452*)

Ref Sequence

ENSEMBL: ENSMUSP00000090355 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000006976] [ENSMUST00000092684]

AlphaFold

Q9D4B2

Predicted Effect

probably null
Transcript: ENSMUST00000006976
AA Change: E452*

SMART Domains

Protein: ENSMUSP00000006976
Gene: ENSMUSG00000006784
AA Change: E452*

Domain	Start	End	E-Value	Type
TPR	11	44	3.69e1	SMART
TPR	45	78	3.07e1	SMART
TPR	79	112	4.96e0	SMART
low complexity region	147	158	N/A	INTRINSIC
low complexity region	191	208	N/A	INTRINSIC
TPR	318	351	4.96e0	SMART
TPR	358	391	1.11e1	SMART
Blast:TPR	395	428	7e-14	BLAST
TPR	435	468	2.99e1	SMART
low complexity region	493	505	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000092684
AA Change: E452*

SMART Domains

Protein: ENSMUSP00000090355
Gene: ENSMUSG00000006784
AA Change: E452*

Domain	Start	End	E-Value	Type
TPR	11	44	3.69e1	SMART
TPR	45	78	3.07e1	SMART
TPR	79	112	4.96e0	SMART
low complexity region	147	158	N/A	INTRINSIC
low complexity region	191	208	N/A	INTRINSIC
TPR	318	351	4.96e0	SMART
TPR	358	391	1.11e1	SMART
Blast:TPR	395	428	5e-14	BLAST
TPR	435	468	2.99e1	SMART
coiled coil region	528	548	N/A	INTRINSIC
low complexity region	556	567	N/A	INTRINSIC
coiled coil region	599	624	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (69/69)

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele display partial preweaning lethality, impaired ciliary motility, and a variety of left-right body symmetry defects. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130401M01Rik	C	T	15: 58,022,509	V326M	probably damaging	Het
Adap2	T	A	11: 80,156,959	H80Q	probably benign	Het
Ang4	T	C	14: 51,764,486	T2A	probably benign	Het
Arf2	T	C	11: 103,979,759		probably null	Het
B3gnt4	A	G	5: 123,511,148	D192G	probably damaging	Het
BC034090	T	C	1: 155,226,357	I54V	probably benign	Het
Catsper3	A	G	13: 55,804,895	T202A	probably benign	Het
Cenpe	T	A	3: 135,260,101	D2113E	probably damaging	Het
Clasp2	T	C	9: 113,773,705	V191A	probably benign	Het
Col5a1	G	T	2: 28,014,158	A1356S	unknown	Het
Cuzd1	A	G	7: 131,308,848	S573P	probably damaging	Het
Cyp1a2	C	A	9: 57,681,885	M215I	possibly damaging	Het
Dcaf17	T	A	2: 71,063,402	I122K	possibly damaging	Het
Dhcr24	A	G	4: 106,573,809	I232V	probably benign	Het
Dnah10	A	G	5: 124,818,117	E3570G	probably damaging	Het
Dnaja4	A	T	9: 54,714,704		probably benign	Het
Ehd1	A	G	19: 6,298,324	D444G	probably damaging	Het
Ehmt1	T	A	2: 24,836,476	M766L	possibly damaging	Het
Eno4	A	G	19: 58,970,722	I613M	probably benign	Het
Exoc3l	A	T	8: 105,290,549	M602K		Het
Fam107a	C	T	14: 8,301,352		probably null	Het
Frem3	A	T	8: 80,613,148	D690V	probably damaging	Het
Gm11639	T	C	11: 104,690,955	I41T	probably benign	Het
Gm19410	A	T	8: 35,771,868	D97V	probably damaging	Het
Grik5	T	A	7: 25,023,064	D540V	possibly damaging	Het
Hint1	T	A	11: 54,869,943	D69E	probably benign	Het
Krt13	T	C	11: 100,119,385	T257A	probably benign	Het
Lpin2	T	C	17: 71,242,754	L676P	probably damaging	Het
Lrguk	A	G	6: 34,029,683	E76G	probably benign	Het
Lrrc8a	A	G	2: 30,256,298	M375V	probably benign	Het
Lrrtm3	T	C	10: 64,089,238	Q50R	possibly damaging	Het
Mmrn1	T	A	6: 60,976,529	L598Q	probably damaging	Het
Mrs2	A	G	13: 25,001,784	I135T	probably damaging	Het
Neb	T	C	2: 52,235,580	D475G		Het
Notch2	A	G	3: 98,135,599	S1427G	possibly damaging	Het
Olfr118	C	A	17: 37,672,411	Y129*	probably null	Het
Olfr682-ps1	A	G	7: 105,126,686	V195A	probably benign	Het
Olfr694	A	T	7: 106,689,089	I214N	probably damaging	Het
Olfr816	A	G	10: 129,911,862	C139R	probably damaging	Het
Olfr952	A	C	9: 39,426,219	V284G	possibly damaging	Het
Opcml	T	C	9: 28,902,151	F246S	probably damaging	Het
Pdzd2	C	A	15: 12,402,319	V729F	probably damaging	Het
Pias2	C	T	18: 77,146,768	Q565*	probably null	Het
Pnp	T	A	14: 50,950,720		probably null	Het
Ptpn18	T	C	1: 34,463,130	S76P	probably benign	Het
Qtrt2	A	T	16: 43,863,197	L304Q	probably damaging	Het
Rad51ap2	A	T	12: 11,457,400	E441V	possibly damaging	Het
Ranbp2	T	A	10: 58,477,889	V1477E	probably benign	Het
Repin1	C	T	6: 48,597,433	T432I	possibly damaging	Het
Rest	G	A	5: 77,282,511	G926R	probably benign	Het
Rnft1	T	A	11: 86,486,690	F143L	possibly damaging	Het
Sema3e	A	T	5: 14,232,094	I415L	probably benign	Het
Slc10a1	T	A	12: 80,967,595	N117I	probably damaging	Het
Slc25a23	G	A	17: 57,059,709		probably benign	Het
Slc2a2	T	C	3: 28,713,802	S160P	possibly damaging	Het
Srrd	A	G	5: 112,338,456	V178A	possibly damaging	Het
Tmigd3	G	T	3: 105,921,961	G198C	probably benign	Het
Trbv29	G	A	6: 41,271,405	M1I	probably null	Het
Tril	A	G	6: 53,819,584	S218P	probably damaging	Het
Trip11	T	C	12: 101,862,598	K1749R	probably benign	Het
Ttn	A	G	2: 76,830,651	V12011A		Het
Ubr4	T	G	4: 139,410,518	F1093V	probably benign	Het
Urb2	C	A	8: 124,028,403	A283E	probably benign	Het
Usp43	T	A	11: 67,898,881		probably benign	Het
Vmn1r28	A	T	6: 58,265,684	I171F	probably benign	Het
Vps33a	G	A	5: 123,533,899	R469W	probably damaging	Het
Zfp202	G	A	9: 40,211,757	R605Q	probably damaging	Het
Zpbp2	A	T	11: 98,554,620	H158L	probably benign	Het

Other mutations in Ttc25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02027:Ttc25	APN	11	100569902	missense	probably damaging	1.00
IGL02379:Ttc25	APN	11	100566983	missense	possibly damaging	0.87
IGL03091:Ttc25	APN	11	100550250	nonsense	probably null
IGL02991:Ttc25	UTSW	11	100553872	missense	probably benign	0.01
R0044:Ttc25	UTSW	11	100567001	missense	probably damaging	1.00
R0137:Ttc25	UTSW	11	100563568	missense	probably damaging	1.00
R0280:Ttc25	UTSW	11	100550265	missense	probably damaging	1.00
R1373:Ttc25	UTSW	11	100545832	missense	probably damaging	1.00
R1793:Ttc25	UTSW	11	100569853	splice site	probably null
R2097:Ttc25	UTSW	11	100563582	missense	possibly damaging	0.89
R2121:Ttc25	UTSW	11	100567011	critical splice donor site	probably null
R2509:Ttc25	UTSW	11	100553535	missense	probably damaging	0.99
R2655:Ttc25	UTSW	11	100553579	missense	probably damaging	1.00
R4635:Ttc25	UTSW	11	100551507	nonsense	probably null
R4773:Ttc25	UTSW	11	100549916	missense	probably benign	0.05
R4858:Ttc25	UTSW	11	100550321	missense	probably damaging	0.96
R5164:Ttc25	UTSW	11	100571520	nonsense	probably null
R5181:Ttc25	UTSW	11	100549893	missense	probably damaging	1.00
R5707:Ttc25	UTSW	11	100554061	missense	probably damaging	0.99
R5742:Ttc25	UTSW	11	100545873	missense	possibly damaging	0.90
R7634:Ttc25	UTSW	11	100561905	critical splice donor site	probably null
R7851:Ttc25	UTSW	11	100545829	missense	probably damaging	1.00
R8186:Ttc25	UTSW	11	100563679	missense	probably benign	0.27
R8194:Ttc25	UTSW	11	100563676	missense	probably benign	0.06
R8444:Ttc25	UTSW	11	100561905	critical splice donor site	probably null
X0018:Ttc25	UTSW	11	100553598	missense	probably damaging	0.99
X0028:Ttc25	UTSW	11	100545898	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TAAAATCGGTCCCCTCACTGAG -3'
(R):5'- CCTGTGTCCTGTGAAGGATG -3'

Sequencing Primer
(F):5'- GTGTCCCAGTCCTCAGTGCTG -3'
(R):5'- TCCTGTGAAGGATGGGCGATAAG -3'

Posted On

2021-07-15