Mutagenetix > Incidental Mutation

Incidental Mutation 'R8881:Or10ak8'

676897

Institutional Source

Beutler Lab

Gene Symbol

Or10ak8

Ensembl Gene

ENSMUSG00000096705

Gene Name

olfactory receptor family 10 subfamily AK member 8

Synonyms

Olfr1329, MOR259-2, GA_x6K02T2QD9B-18619941-18620882

MMRRC Submission

068749-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.138) question?

Stock #

R8881 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

118773721-118774662 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 118774571 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 31 (V31A)

Ref Sequence

ENSEMBL: ENSMUSP00000071870 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071979]

AlphaFold

L7MTV4

Predicted Effect

probably benign
Transcript: ENSMUST00000071979
AA Change: V31A

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000071870
Gene: ENSMUSG00000096705
AA Change: V31A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	308	4.3e-55	PFAM
Pfam:7TM_GPCR_Srsx	36	306	1.5e-6	PFAM
Pfam:7tm_1	42	291	5.7e-25	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.5%

Validation Efficiency

100% (69/69)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca16	G	A	7: 120,074,794 (GRCm39)	C612Y	probably benign	Het
Adam21	T	C	12: 81,606,650 (GRCm39)	T371A	probably benign	Het
Akap9	T	C	5: 4,011,279 (GRCm39)	S661P		Het
Anxa11	C	T	14: 25,874,687 (GRCm39)	R233C	probably damaging	Het
Capza3	A	C	6: 139,987,521 (GRCm39)	D40A	probably damaging	Het
Caskin1	C	A	17: 24,718,273 (GRCm39)	N294K	probably damaging	Het
Cdh1	T	C	8: 107,392,904 (GRCm39)	V796A	probably benign	Het
Cfap251	C	T	5: 123,462,438 (GRCm39)	P1249L	probably damaging	Het
Cped1	A	T	6: 22,119,578 (GRCm39)	T346S	possibly damaging	Het
Crisp4	A	T	1: 18,185,902 (GRCm39)	V278D	probably damaging	Het
Ddx60	A	C	8: 62,474,343 (GRCm39)	D1477A	possibly damaging	Het
Dmrtb1	T	C	4: 107,537,922 (GRCm39)	D82G	possibly damaging	Het
Dnah11	T	C	12: 118,077,647 (GRCm39)	N1282S	probably benign	Het
Dnah11	C	T	12: 118,090,550 (GRCm39)	V1104M	probably benign	Het
Dot1l	A	G	10: 80,621,429 (GRCm39)	E630G	probably damaging	Het
Dppa4	C	A	16: 48,108,299 (GRCm39)	T28K		Het
Dusp5	T	A	19: 53,529,745 (GRCm39)	S383T	probably benign	Het
Eef2k	C	T	7: 120,472,548 (GRCm39)	A87V	probably damaging	Het
Epha1	T	C	6: 42,337,961 (GRCm39)	Y828C	probably damaging	Het
Erbb4	A	C	1: 68,382,997 (GRCm39)		probably null	Het
Fam151b	T	A	13: 92,604,630 (GRCm39)	M120L	probably benign	Het
Fam237b	A	C	5: 5,625,379 (GRCm39)	D25A	possibly damaging	Het
Fez2	T	C	17: 78,689,051 (GRCm39)	D366G	probably damaging	Het
Frmd4b	G	T	6: 97,272,735 (GRCm39)	H886N	probably benign	Het
Galnt17	T	C	5: 130,906,635 (GRCm39)	H511R	probably benign	Het
Grpel1	C	A	5: 36,626,816 (GRCm39)	Q33K	possibly damaging	Het
Gzmm	A	C	10: 79,530,819 (GRCm39)		probably null	Het
Hmcn1	C	A	1: 150,525,723 (GRCm39)	L3333F	probably damaging	Het
Il21r	A	T	7: 125,231,498 (GRCm39)	T309S	probably benign	Het
Itgal	G	A	7: 126,929,541 (GRCm39)	V1153M	probably benign	Het
Kdf1	G	A	4: 133,257,654 (GRCm39)	A390T	possibly damaging	Het
Lcn11	G	T	2: 25,669,296 (GRCm39)	R151L	probably benign	Het
Lgi3	T	A	14: 70,770,282 (GRCm39)	Y116N	probably damaging	Het
Llgl2	A	G	11: 115,743,866 (GRCm39)	H731R	probably benign	Het
Lrp5	A	T	19: 3,641,015 (GRCm39)	S1482T	probably damaging	Het
Meis2	T	C	2: 115,889,116 (GRCm39)	D212G	probably benign	Het
Mrgpra9	A	G	7: 46,885,242 (GRCm39)	C142R	possibly damaging	Het
Mrgprf	A	G	7: 144,861,999 (GRCm39)	H187R	probably benign	Het
Mvd	A	G	8: 123,164,564 (GRCm39)		probably null	Het
Neb	T	C	2: 52,096,999 (GRCm39)	I4938M	possibly damaging	Het
Nipal4	A	T	11: 46,042,177 (GRCm39)	M168K	probably benign	Het
Nr1h4	A	G	10: 89,319,351 (GRCm39)	Y158H	probably damaging	Het
Nrxn2	T	C	19: 6,554,920 (GRCm39)	I1133T	probably benign	Het
Numa1	T	C	7: 101,650,684 (GRCm39)	Y1472H	probably benign	Het
Or12j3	A	G	7: 139,952,698 (GRCm39)	V275A	probably benign	Het
Or2y10	G	A	11: 49,455,209 (GRCm39)	V154M	probably benign	Het
Or3a1d	T	C	11: 74,237,471 (GRCm39)	D313G	probably benign	Het
Or52h7	A	G	7: 104,213,619 (GRCm39)	M64V	possibly damaging	Het
Pate7	T	A	9: 35,689,384 (GRCm39)		probably benign	Het
Pcdha6	G	T	18: 37,101,484 (GRCm39)	V226F	probably damaging	Het
Pnpla6	G	A	8: 3,581,489 (GRCm39)	M605I	probably benign	Het
Ppp1r13l	G	A	7: 19,105,194 (GRCm39)	R322H	probably damaging	Het
Sgtb	T	C	13: 104,258,046 (GRCm39)		probably null	Het
Skint6	T	C	4: 112,672,716 (GRCm39)	K1086E	possibly damaging	Het
Slc4a11	T	C	2: 130,527,457 (GRCm39)	E646G	probably damaging	Het
Speg	G	T	1: 75,377,795 (GRCm39)	R851L	possibly damaging	Het
Spns3	T	G	11: 72,429,912 (GRCm39)	D172A	probably damaging	Het
Srgap3	A	G	6: 112,700,098 (GRCm39)	V984A	probably benign	Het
Syne1	A	T	10: 5,223,639 (GRCm39)	D3080E	probably damaging	Het
Taf5l	T	C	8: 124,730,101 (GRCm39)	H196R	possibly damaging	Het
Tmem229a	G	C	6: 24,955,587 (GRCm39)	R56G	probably damaging	Het
Tmprss6	A	C	15: 78,327,987 (GRCm39)	*582G	probably null	Het
Trip13	C	A	13: 74,077,795 (GRCm39)	R173L	possibly damaging	Het
Trpm7	A	T	2: 126,661,883 (GRCm39)	V1055E	probably damaging	Het
Utrn	A	G	10: 12,423,737 (GRCm39)	I2T	possibly damaging	Het
V1rd19	T	A	7: 23,703,081 (GRCm39)	S182R	possibly damaging	Het
Vmn2r58	C	A	7: 41,486,609 (GRCm39)	G762V	probably benign	Het
Zfp874b	T	C	13: 67,622,141 (GRCm39)	K386E	probably damaging	Het

Other mutations in Or10ak8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01064:Or10ak8	APN	4	118,774,091 (GRCm39)	missense	possibly damaging	0.67
IGL02065:Or10ak8	APN	4	118,773,968 (GRCm39)	missense	probably benign	0.00
IGL02085:Or10ak8	APN	4	118,773,947 (GRCm39)	missense	probably damaging	1.00
IGL02092:Or10ak8	APN	4	118,774,187 (GRCm39)	missense	possibly damaging	0.72
IGL02557:Or10ak8	APN	4	118,774,389 (GRCm39)	missense	probably benign	0.00
R0129:Or10ak8	UTSW	4	118,774,667 (GRCm39)	splice site	probably null
R0411:Or10ak8	UTSW	4	118,773,823 (GRCm39)	missense	possibly damaging	0.87
R5001:Or10ak8	UTSW	4	118,774,440 (GRCm39)	missense	probably damaging	0.97
R5313:Or10ak8	UTSW	4	118,773,995 (GRCm39)	missense	probably benign	0.00
R5733:Or10ak8	UTSW	4	118,774,035 (GRCm39)	missense	probably benign	0.00
R6153:Or10ak8	UTSW	4	118,773,944 (GRCm39)	missense	probably damaging	1.00
R6274:Or10ak8	UTSW	4	118,774,427 (GRCm39)	missense	probably benign	0.01
R7671:Or10ak8	UTSW	4	118,774,183 (GRCm39)	missense	probably benign	0.02
R8358:Or10ak8	UTSW	4	118,773,723 (GRCm39)	makesense	probably null
R8696:Or10ak8	UTSW	4	118,774,635 (GRCm39)	missense	probably benign	0.23
R9599:Or10ak8	UTSW	4	118,773,851 (GRCm39)	missense	probably benign	0.00
R9655:Or10ak8	UTSW	4	118,773,804 (GRCm39)	missense	probably damaging	1.00
Z1088:Or10ak8	UTSW	4	118,773,785 (GRCm39)	missense	probably damaging	1.00
Z1176:Or10ak8	UTSW	4	118,774,332 (GRCm39)	missense	probably damaging	0.96
Z1176:Or10ak8	UTSW	4	118,774,224 (GRCm39)	missense	probably benign	0.21

Predicted Primers

PCR Primer
(F):5'- CCAGCAAAGGAGATGGTCTG -3'
(R):5'- CATGATCCAGAGCCATGAAGG -3'

Sequencing Primer
(F):5'- TCTGAGAGTGAGCAAGAAGATGC -3'
(R):5'- CTTGAGGATCCAAGTTACTGACC -3'

Posted On

2021-07-15