Mutagenetix > Incidental Mutation

Incidental Mutation 'R8882:Vmn2r60'

676978

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r60

Ensembl Gene

ENSMUSG00000090619

Gene Name

vomeronasal 2, receptor 60

Synonyms

Gprc2a-rs3, Casr-rs3, EG637898

MMRRC Submission

068750-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.113) question?

Stock #

R8882 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

42116471-42195776 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 42141094 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Lysine at position 502 (Q502K)

Ref Sequence

ENSEMBL: ENSMUSP00000128493 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166447]

AlphaFold

A0A3B2WBC8

Predicted Effect

probably benign
Transcript: ENSMUST00000166447
AA Change: Q502K

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000128493
Gene: ENSMUSG00000090619
AA Change: Q502K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	78	471	1.2e-44	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	1.4e-51	PFAM

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.5%

Validation Efficiency

100% (55/55)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actbl2	A	T	13: 111,255,499	I123L	probably benign	Het
Adam18	T	C	8: 24,646,422	D361G	probably benign	Het
Adcyap1r1	G	A	6: 55,491,234	V352M	possibly damaging	Het
Ahnak	T	C	19: 9,000,742	L75P	probably damaging	Het
Arhgap23	T	C	11: 97,465,123	S856P	probably benign	Het
Asprv1	G	T	6: 86,628,367	C65F	probably benign	Het
Atp2b4	A	T	1: 133,726,455		probably null	Het
Cd55	A	G	1: 130,459,764	V99A	probably benign	Het
Cit	G	A	5: 115,863,030	A163T	probably benign	Het
Dnah6	A	T	6: 73,178,498	D711E	probably benign	Het
Dnajc15	A	T	14: 77,856,971	V41E	probably damaging	Het
Dock2	GCACACACACA	GCACACACACACA	11: 34,704,609	453	probably null	Het
Dst	T	C	1: 34,200,924	S1785P	probably damaging	Het
Efhb	A	G	17: 53,462,684	V199A	probably damaging	Het
Esyt3	C	T	9: 99,320,856	G498D	probably damaging	Het
Fbxo11	A	G	17: 87,997,529	I562T		Het
Fh1	C	T	1: 175,609,787	V249I	possibly damaging	Het
Gdpgp1	T	C	7: 80,238,956	I245T	possibly damaging	Het
Hinfp	A	G	9: 44,298,332		probably null	Het
Hip1r	A	G	5: 124,001,962	K1043E	probably damaging	Het
Htt	G	A	5: 34,821,717	V815I	probably benign	Het
Jrk	A	G	15: 74,707,155	Y94H	probably damaging	Het
Krit1	T	A	5: 3,836,864	N704K	possibly damaging	Het
Mmp17	A	G	5: 129,601,944	D331G	probably benign	Het
Nlrp9a	T	A	7: 26,558,278	Y440*	probably null	Het
Nphp3	A	G	9: 104,005,594	T216A	possibly damaging	Het
Olfr1252	G	T	2: 89,721,396	C238*	probably null	Het
Olfr291	T	C	7: 84,856,473	Y37H	probably damaging	Het
Olfr397	T	G	11: 73,965,114	C169G	probably damaging	Het
Olfr872	T	C	9: 20,259,960	F40S	probably benign	Het
Olfr893	T	A	9: 38,209,165	Y35*	probably null	Het
Parva	T	C	7: 112,428,004	S14P	probably benign	Het
Pax6	A	C	2: 105,691,618	N207H	possibly damaging	Het
Pde6a	A	G	18: 61,245,548	N314S		Het
Phlpp1	T	A	1: 106,392,642	S1456T	probably benign	Het
Plxnc1	A	G	10: 94,841,566	V933A	probably damaging	Het
Rasgef1b	A	G	5: 99,377,001	S100P	probably benign	Het
Rpn2	A	G	2: 157,294,182	H170R	probably benign	Het
Sbds	A	G	5: 130,253,937		probably null	Het
Scube2	G	C	7: 109,852,473	L158V	probably damaging	Het
Slc16a12	C	A	19: 34,672,454	V394L	probably benign	Het
Slc23a2	A	T	2: 132,091,239	Y100N	possibly damaging	Het
Slc30a9	G	T	5: 67,315,701	E43*	probably null	Het
Specc1l	A	T	10: 75,229,855	M1L	unknown	Het
Sspo	T	A	6: 48,475,456	C2785S	probably damaging	Het
Star	T	C	8: 25,812,869	S280P	probably benign	Het
Strip1	A	T	3: 107,627,025	M127K	probably benign	Het
Tbx19	C	T	1: 165,139,211	V365M	probably benign	Het
Tex2	T	C	11: 106,544,236	D788G	unknown	Het
Tspyl1	A	G	10: 34,282,498	E73G	possibly damaging	Het
Ulk2	C	T	11: 61,808,061		probably null	Het
Vmn2r120	A	T	17: 57,545,229	M29K	probably benign	Het
Xpo5	A	G	17: 46,227,740	D624G	possibly damaging	Het
Zfp119b	T	C	17: 55,939,923	R88G	possibly damaging	Het
Zfp879	C	A	11: 50,833,936	E98*	probably null	Het

Other mutations in Vmn2r60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01622:Vmn2r60	APN	7	42136486	missense	probably benign	0.09
IGL01623:Vmn2r60	APN	7	42136486	missense	probably benign	0.09
IGL02363:Vmn2r60	APN	7	42195154	missense	probably benign	0.02
IGL02485:Vmn2r60	APN	7	42195466	missense	possibly damaging	0.54
IGL02651:Vmn2r60	APN	7	42195586	missense	probably damaging	0.99
IGL02660:Vmn2r60	APN	7	42142296	nonsense	probably null
IGL03135:Vmn2r60	APN	7	42136594	missense	probably benign	0.13
IGL03307:Vmn2r60	APN	7	42116547	missense	probably benign	0.14
R0310:Vmn2r60	UTSW	7	42195140	missense	possibly damaging	0.54
R0314:Vmn2r60	UTSW	7	42135561	splice site	probably benign
R0328:Vmn2r60	UTSW	7	42142320	splice site	probably benign
R0464:Vmn2r60	UTSW	7	42135831	missense	probably damaging	0.99
R0755:Vmn2r60	UTSW	7	42195445	missense	probably damaging	1.00
R1119:Vmn2r60	UTSW	7	42194941	missense	possibly damaging	0.68
R1162:Vmn2r60	UTSW	7	42195771	missense	probably benign	0.29
R1241:Vmn2r60	UTSW	7	42137052	missense	probably benign	0.01
R1404:Vmn2r60	UTSW	7	42136787	missense	probably damaging	0.99
R1404:Vmn2r60	UTSW	7	42136787	missense	probably damaging	0.99
R1488:Vmn2r60	UTSW	7	42136713	missense	probably benign	0.17
R1623:Vmn2r60	UTSW	7	42135855	nonsense	probably null
R1628:Vmn2r60	UTSW	7	42136406	nonsense	probably null
R1883:Vmn2r60	UTSW	7	42136670	missense	probably damaging	0.99
R1884:Vmn2r60	UTSW	7	42136670	missense	probably damaging	0.99
R2182:Vmn2r60	UTSW	7	42195507	missense	probably benign	0.06
R2275:Vmn2r60	UTSW	7	42136827	nonsense	probably null
R2847:Vmn2r60	UTSW	7	42136433	missense	probably benign	0.07
R2885:Vmn2r60	UTSW	7	42140979	missense	possibly damaging	0.91
R2894:Vmn2r60	UTSW	7	42135796	missense	probably benign
R2921:Vmn2r60	UTSW	7	42141035	missense	probably damaging	0.98
R2922:Vmn2r60	UTSW	7	42141035	missense	probably damaging	0.98
R3772:Vmn2r60	UTSW	7	42116556	missense	probably benign	0.35
R3820:Vmn2r60	UTSW	7	42135701	missense	probably damaging	0.98
R3822:Vmn2r60	UTSW	7	42135701	missense	probably damaging	0.98
R3872:Vmn2r60	UTSW	7	42136454	missense	probably benign	0.19
R4222:Vmn2r60	UTSW	7	42116528	missense	probably benign	0.08
R4223:Vmn2r60	UTSW	7	42116528	missense	probably benign	0.08
R4224:Vmn2r60	UTSW	7	42116528	missense	probably benign	0.08
R4526:Vmn2r60	UTSW	7	42195243	missense	probably damaging	0.96
R4547:Vmn2r60	UTSW	7	42135663	missense	probably null	0.54
R4840:Vmn2r60	UTSW	7	42135861	missense	probably damaging	1.00
R5173:Vmn2r60	UTSW	7	42195511	missense	probably damaging	0.97
R5231:Vmn2r60	UTSW	7	42137024	missense	possibly damaging	0.93
R5480:Vmn2r60	UTSW	7	42135730	missense	probably damaging	0.98
R5521:Vmn2r60	UTSW	7	42195625	missense	probably damaging	0.99
R5834:Vmn2r60	UTSW	7	42116508	missense	probably benign	0.17
R6038:Vmn2r60	UTSW	7	42194962	missense	probably benign	0.04
R6038:Vmn2r60	UTSW	7	42194962	missense	probably benign	0.04
R6112:Vmn2r60	UTSW	7	42195423	missense	probably damaging	1.00
R6149:Vmn2r60	UTSW	7	42136976	missense	probably damaging	1.00
R6170:Vmn2r60	UTSW	7	42135621	missense	possibly damaging	0.94
R6383:Vmn2r60	UTSW	7	42116471	start codon destroyed	probably null	0.04
R6811:Vmn2r60	UTSW	7	42194886	missense	probably damaging	1.00
R6876:Vmn2r60	UTSW	7	42135663	missense	probably null	0.54
R6997:Vmn2r60	UTSW	7	42142292	missense	probably benign	0.00
R7040:Vmn2r60	UTSW	7	42142242	missense	probably benign	0.00
R7116:Vmn2r60	UTSW	7	42137063	missense	probably benign	0.00
R7128:Vmn2r60	UTSW	7	42195112	missense	probably damaging	0.96
R7232:Vmn2r60	UTSW	7	42136742	missense	possibly damaging	0.83
R7296:Vmn2r60	UTSW	7	42136402	missense	probably benign	0.01
R7376:Vmn2r60	UTSW	7	42195207	missense	probably damaging	1.00
R7526:Vmn2r60	UTSW	7	42195734	frame shift	probably null
R7527:Vmn2r60	UTSW	7	42195734	frame shift	probably null
R7528:Vmn2r60	UTSW	7	42195734	frame shift	probably null
R7764:Vmn2r60	UTSW	7	42195111	missense	probably damaging	0.99
R7843:Vmn2r60	UTSW	7	42195087	missense	probably benign	0.00
R8080:Vmn2r60	UTSW	7	42141097	missense	probably benign	0.30
R8290:Vmn2r60	UTSW	7	42142266	missense	probably damaging	1.00
R8342:Vmn2r60	UTSW	7	42141070	missense	possibly damaging	0.63
R8362:Vmn2r60	UTSW	7	42195530	missense	probably damaging	1.00
R8418:Vmn2r60	UTSW	7	42195426	missense	probably damaging	0.97
R8848:Vmn2r60	UTSW	7	42136745	missense	probably damaging	1.00
R8860:Vmn2r60	UTSW	7	42142230	missense	probably damaging	0.99
R8913:Vmn2r60	UTSW	7	42136354	missense	probably benign	0.27
R9190:Vmn2r60	UTSW	7	42195511	missense	probably damaging	0.99
R9229:Vmn2r60	UTSW	7	42142299	missense	possibly damaging	0.95
R9295:Vmn2r60	UTSW	7	42136531	missense	probably benign	0.01
R9335:Vmn2r60	UTSW	7	42194908	missense	probably damaging	1.00
R9796:Vmn2r60	UTSW	7	42135748	missense	probably benign
RF024:Vmn2r60	UTSW	7	42140939	missense	probably benign	0.01
X0023:Vmn2r60	UTSW	7	42141114	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GCTTCACCCTTTCCTAAAGAAC -3'
(R):5'- TTCCGAGGCAGTAACTAATAGACAC -3'

Sequencing Primer
(F):5'- CCCTTTCCTAAAGAACACGATAATG -3'
(R):5'- ATGGTCATGACATCCTGA -3'

Posted On

2021-07-15