Mutagenetix > Incidental Mutation

Incidental Mutation 'T0975:Or6c35'

67727

Institutional Source

Beutler Lab

Gene Symbol

Or6c35

Ensembl Gene

ENSMUSG00000095138

Gene Name

olfactory receptor family 6 subfamily C member 35

Synonyms

MOR114-6, Olfr781, GA_x6K02T2PULF-11013616-11014551

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

T0975 (G3) of strain 714

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

129168752-129169687 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 129169314 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 188 (D188G)

Ref Sequence

ENSEMBL: ENSMUSP00000145356 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075613] [ENSMUST00000204108]

AlphaFold

Q8VFZ8

Predicted Effect

probably benign
Transcript: ENSMUST00000075613
AA Change: D188G

PolyPhen 2 Score 0.307 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000075041
Gene: ENSMUSG00000095138
AA Change: D188G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	305	1.6e-44	PFAM
Pfam:7tm_1	38	287	5e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000204108
AA Change: D188G

PolyPhen 2 Score 0.307 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000145356
Gene: ENSMUSG00000095138
AA Change: D188G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	305	1.6e-44	PFAM
Pfam:7tm_1	38	287	5e-24	PFAM

Coding Region Coverage

1x: 99.4%
3x: 98.7%
10x: 97.0%
20x: 93.3%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930556J24Rik	TAA	TAAA	11: 3,887,945 (GRCm39)		probably null	Het
4930556J24Rik	C	T	11: 3,926,324 (GRCm39)	A27T	unknown	Het
Ago3	C	T	4: 126,298,056 (GRCm39)	V155I	probably benign	Het
Ago3	G	A	4: 126,298,103 (GRCm39)	A139V	probably benign	Het
Ago3	C	T	4: 126,298,098 (GRCm39)	A141T	probably benign	Het
Ahdc1	ACCTCCT	ACCTCCTCCT	4: 132,790,065 (GRCm39)		probably benign	Het
Azin2	A	G	4: 128,839,927 (GRCm39)	Y222H	probably benign	Het
Bpifb5	C	A	2: 154,071,384 (GRCm39)		probably null	Het
Castor1	G	C	11: 4,170,445 (GRCm39)	G147A	probably benign	Het
Ccdc157	C	T	11: 4,096,246 (GRCm39)	A455T	probably damaging	Het
Ccng1	A	C	11: 40,644,871 (GRCm39)	S9A	probably benign	Het
Cfh	T	C	1: 140,082,336 (GRCm39)	T164A	probably benign	Het
Cherp	TTGGACCTGGACCTGGACCTGGACCTGGA	TTGGACCTGGACCTGGACCTGGA	8: 73,215,878 (GRCm39)		probably benign	Het
Chrng	T	C	1: 87,138,348 (GRCm39)	S380P	probably benign	Het
Clspn	ACGGCGGCGGCGGCG	ACGGCGGCGGCGGCGGCGGCG	4: 126,460,230 (GRCm39)		probably benign	Het
Ctrc	T	TA	4: 141,572,507 (GRCm39)		probably null	Het
Cxxc1	C	T	18: 74,353,992 (GRCm39)	R593C	probably damaging	Het
Dlgap1	T	C	17: 70,823,950 (GRCm39)	S312P	possibly damaging	Het
Dnah10	A	G	5: 124,840,130 (GRCm39)	S1255G	probably benign	Het
Dpep1	A	T	8: 123,927,727 (GRCm39)	S388C	probably damaging	Het
Emid1	T	C	11: 5,094,386 (GRCm39)	T42A	probably damaging	Het
Emid1	A	C	11: 5,078,884 (GRCm39)	L353V	probably benign	Het
Epn3	A	G	11: 94,382,733 (GRCm39)		probably null	Het
Fam124b	T	C	1: 80,190,843 (GRCm39)	E180G	probably benign	Het
Fam135b	T	G	15: 71,335,734 (GRCm39)	T487P	probably damaging	Het
Gja4	G	C	4: 127,206,024 (GRCm39)	H246Q	probably benign	Het
Gm9972	GA	GAA	11: 42,927,597 (GRCm39)		probably null	Het
Hmmr	G	C	11: 40,614,243 (GRCm39)	N148K	probably damaging	Het
Homez	C	T	14: 55,094,796 (GRCm39)	R304K	possibly damaging	Het
Ifngr1	G	A	10: 19,485,221 (GRCm39)	V407M	probably damaging	Het
Inpp5j	G	T	11: 3,452,527 (GRCm39)	T241N	possibly damaging	Het
Kif12	GGGGC	GGGGCCTCCACCCGGCGGGC	4: 63,089,660 (GRCm39)		probably benign	Het
Kremen1	C	T	11: 5,145,105 (GRCm39)	A424T	probably benign	Het
Mat2b	G	A	11: 40,570,918 (GRCm39)	T302I	probably benign	Het
Mtmr3	C	T	11: 4,438,441 (GRCm39)	R671K	probably benign	Het
Nacad	A	G	11: 6,551,632 (GRCm39)	C520R	probably benign	Het
Nacad	GCAGGGTCAGGGTC	GCAGGGTCAGGGTCAGGGTC	11: 6,549,750 (GRCm39)		probably benign	Het
Nacad	T	C	11: 6,551,622 (GRCm39)	N523S	probably benign	Het
Nefh	G	A	11: 4,890,151 (GRCm39)	P823S	probably benign	Het
Nfrkb	G	C	9: 31,308,379 (GRCm39)	A230P	probably benign	Het
Nlrp4a	A	G	7: 26,149,062 (GRCm39)	E223G	probably damaging	Het
Notch3	T	A	17: 32,365,391 (GRCm39)	Y1107F	probably damaging	Het
Or10ak9	G	T	4: 118,726,500 (GRCm39)	R174M	probably benign	Het
Or13g1	A	T	7: 85,955,492 (GRCm39)	Y276*	probably null	Het
Osm	A	G	11: 4,189,588 (GRCm39)	D124G	probably benign	Het
Plekhm2	TTCCTCCTCCT	TTCCTCCT	4: 141,359,292 (GRCm39)		probably benign	Het
Pomgnt1	C	T	4: 115,994,624 (GRCm39)		probably benign	Het
Spen	A	G	4: 141,201,664 (GRCm39)	V2321A	probably benign	Het
Sytl1	TCTGC	TC	4: 132,984,305 (GRCm39)		probably benign	Het
Tcn2	G	C	11: 3,873,487 (GRCm39)	F286L	possibly damaging	Het
Tg	T	C	15: 66,560,712 (GRCm39)	S10P	probably benign	Het
Tmprss7	C	T	16: 45,501,096 (GRCm39)	R235Q	probably benign	Het
Tns3	G	A	11: 8,499,100 (GRCm39)		probably benign	Het
Tns3	G	T	11: 8,401,146 (GRCm39)	L1051M	probably benign	Het
Tns3	T	G	11: 8,429,518 (GRCm39)	E806A	probably benign	Het
Toe1	T	C	4: 116,663,290 (GRCm39)	I62M	probably benign	Het
Txnrd2	A	G	16: 18,294,315 (GRCm39)	H436R	probably damaging	Het
Ubr4	C	T	4: 139,179,092 (GRCm39)	P2001S	probably damaging	Het
Vmn2r23	T	A	6: 123,690,120 (GRCm39)	M332K	probably benign	Het
Zbtb8a	GG	GGATG	4: 129,253,812 (GRCm39)		probably benign	Het
Zbtb8a	T	C	4: 129,254,005 (GRCm39)	H163R	probably benign	Het
Zfyve21	A	G	12: 111,794,067 (GRCm39)	D206G	probably damaging	Het
Zkscan4	AGAGGAG	AGAG	13: 21,663,370 (GRCm39)		probably benign	Het
Zmym1	A	C	4: 126,943,466 (GRCm39)	H307Q	probably benign	Het
Zmym1	C	T	4: 126,941,740 (GRCm39)	D785N	probably benign	Het
Zmym1	C	T	4: 126,942,043 (GRCm39)	V684I	probably benign	Het
Zpld2	GTG	GTGCTG	4: 133,929,940 (GRCm39)		probably benign	Het

Other mutations in Or6c35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01121:Or6c35	APN	10	129,168,804 (GRCm39)	missense	probably benign
IGL01636:Or6c35	APN	10	129,168,752 (GRCm39)	start codon destroyed	probably damaging	0.96
IGL01744:Or6c35	APN	10	129,169,326 (GRCm39)	missense	probably benign	0.05
IGL01867:Or6c35	APN	10	129,169,232 (GRCm39)	missense	probably damaging	1.00
IGL02002:Or6c35	APN	10	129,168,996 (GRCm39)	missense	probably damaging	0.99
IGL02423:Or6c35	APN	10	129,169,397 (GRCm39)	missense	probably benign	0.00
R2036:Or6c35	UTSW	10	129,169,541 (GRCm39)	missense	probably benign	0.43
R2099:Or6c35	UTSW	10	129,169,152 (GRCm39)	missense	probably damaging	0.96
R2273:Or6c35	UTSW	10	129,169,326 (GRCm39)	missense	probably benign	0.05
R2274:Or6c35	UTSW	10	129,169,326 (GRCm39)	missense	probably benign	0.05
R3841:Or6c35	UTSW	10	129,169,202 (GRCm39)	missense	probably benign	0.00
R4585:Or6c35	UTSW	10	129,169,142 (GRCm39)	missense	probably benign	0.08
R4586:Or6c35	UTSW	10	129,169,142 (GRCm39)	missense	probably benign	0.08
R5522:Or6c35	UTSW	10	129,168,798 (GRCm39)	missense	probably damaging	0.98
R6052:Or6c35	UTSW	10	129,169,071 (GRCm39)	missense	possibly damaging	0.91
R6414:Or6c35	UTSW	10	129,169,578 (GRCm39)	missense	probably benign	0.23
R6468:Or6c35	UTSW	10	129,169,580 (GRCm39)	missense	possibly damaging	0.91
R6647:Or6c35	UTSW	10	129,169,033 (GRCm39)	nonsense	probably null
R8099:Or6c35	UTSW	10	129,168,996 (GRCm39)	missense	probably damaging	0.99
R9151:Or6c35	UTSW	10	129,169,623 (GRCm39)	missense	probably damaging	1.00
R9617:Or6c35	UTSW	10	129,168,794 (GRCm39)	missense	probably damaging	0.98
R9649:Or6c35	UTSW	10	129,169,368 (GRCm39)	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- CAGATGATATTCGGCTGCAAATTCTGC -3'
(R):5'- ACATCGTCCTTGGATGAAGGTTTGAC -3'

Sequencing Primer
(F):5'- TCATCTGGGGACAAGTCCATTAC -3'
(R):5'- TGACATAAATGAAGATGCAGCTACC -3'

Posted On

2013-09-03