Mutagenetix > Incidental Mutation

Incidental Mutation 'T0975:Tg'

67733

Institutional Source

Beutler Lab

Gene Symbol

Ensembl Gene

ENSMUSG00000053469

Gene Name

thyroglobulin

Synonyms

Tgn

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.128) question?

Stock #

T0975 (G3) of strain 714

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

66670753-66850721 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 66688863 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 10 (S10P)

Ref Sequence

ENSEMBL: ENSMUSP00000129868 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065916] [ENSMUST00000163495]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000065916
AA Change: S1025P

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000070239
Gene: ENSMUSG00000053469
AA Change: S1025P

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
TY	50	97	5.9e-16	SMART
TY	118	165	5.59e-17	SMART
Pfam:Thyroglobulin_1	174	252	4e-9	PFAM
TY	317	363	4.36e-19	SMART
low complexity region	495	504	N/A	INTRINSIC
TY	617	662	3.58e-15	SMART
TY	684	730	1.47e-16	SMART
TY	880	926	1.51e-4	SMART
TY	1029	1078	1.21e-12	SMART
TY	1106	1150	7.56e-5	SMART
TY	1167	1215	7.26e-16	SMART
low complexity region	1244	1255	N/A	INTRINSIC
Pfam:GCC2_GCC3	1464	1509	2.7e-16	PFAM
TY	1519	1568	9.81e-13	SMART
Pfam:COesterase	2181	2717	8.4e-140	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000163495
AA Change: S10P

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000129868
Gene: ENSMUSG00000053469
AA Change: S10P

Domain	Start	End	E-Value	Type
TY	14	63	1.21e-12	SMART

Meta Mutation Damage Score

0.1434

Coding Region Coverage

1x: 99.4%
3x: 98.7%
10x: 97.0%
20x: 93.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]
PHENOTYPE: Mice homozygous for a spontaneous mutation exhibit enlarged thyroid gland, hypothyroidism, abnormal thyroid gland morphology, and decreased body weight. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930556J24Rik	C	T	11: 3,976,324 (GRCm38)	A27T	unknown	Het
4930556J24Rik	TAA	TAAA	11: 3,937,945 (GRCm38)		probably null	Het
Ago3	C	T	4: 126,404,263 (GRCm38)	V155I	probably benign	Het
Ago3	C	T	4: 126,404,305 (GRCm38)	A141T	probably benign	Het
Ago3	G	A	4: 126,404,310 (GRCm38)	A139V	probably benign	Het
Ahdc1	ACCTCCT	ACCTCCTCCT	4: 133,062,754 (GRCm38)		probably benign	Het
Azin2	A	G	4: 128,946,134 (GRCm38)	Y222H	probably benign	Het
Bpifb5	C	A	2: 154,229,464 (GRCm38)		probably null	Het
Ccdc157	C	T	11: 4,146,246 (GRCm38)	A455T	probably damaging	Het
Ccng1	A	C	11: 40,754,044 (GRCm38)	S9A	probably benign	Het
Cfh	T	C	1: 140,154,598 (GRCm38)	T164A	probably benign	Het
Cherp	TTGGACCTGGACCTGGACCTGGACCTGGA	TTGGACCTGGACCTGGACCTGGA	8: 72,462,034 (GRCm38)		probably benign	Het
Chrng	T	C	1: 87,210,626 (GRCm38)	S380P	probably benign	Het
Clspn	ACGGCGGCGGCGGCG	ACGGCGGCGGCGGCGGCGGCG	4: 126,566,437 (GRCm38)		probably benign	Het
Ctrc	T	TA	4: 141,845,196 (GRCm38)		probably null	Het
Cxxc1	C	T	18: 74,220,921 (GRCm38)	R593C	probably damaging	Het
Dlgap1	T	C	17: 70,516,955 (GRCm38)	S312P	possibly damaging	Het
Dnah10	A	G	5: 124,763,066 (GRCm38)	S1255G	probably benign	Het
Dpep1	A	T	8: 123,200,988 (GRCm38)	S388C	probably damaging	Het
Emid1	T	C	11: 5,144,386 (GRCm38)	T42A	probably damaging	Het
Emid1	A	C	11: 5,128,884 (GRCm38)	L353V	probably benign	Het
Epn3	A	G	11: 94,491,907 (GRCm38)		probably null	Het
Fam124b	T	C	1: 80,213,126 (GRCm38)	E180G	probably benign	Het
Fam135b	T	G	15: 71,463,885 (GRCm38)	T487P	probably damaging	Het
Gatsl3	G	C	11: 4,220,445 (GRCm38)	G147A	probably benign	Het
Gja4	G	C	4: 127,312,231 (GRCm38)	H246Q	probably benign	Het
Gm7534	GTG	GTGCTG	4: 134,202,629 (GRCm38)		probably benign	Het
Gm9972	GA	GAA	11: 43,036,770 (GRCm38)		probably null	Het
Hmmr	G	C	11: 40,723,416 (GRCm38)	N148K	probably damaging	Het
Homez	C	T	14: 54,857,339 (GRCm38)	R304K	possibly damaging	Het
Ifngr1	G	A	10: 19,609,473 (GRCm38)	V407M	probably damaging	Het
Inpp5j	G	T	11: 3,502,527 (GRCm38)	T241N	possibly damaging	Het
Kif12	GGGGC	GGGGCCTCCACCCGGCGGGC	4: 63,171,423 (GRCm38)		probably benign	Het
Kremen1	C	T	11: 5,195,105 (GRCm38)	A424T	probably benign	Het
Mat2b	G	A	11: 40,680,091 (GRCm38)	T302I	probably benign	Het
Mtmr3	C	T	11: 4,488,441 (GRCm38)	R671K	probably benign	Het
Nacad	GCAGGGTCAGGGTC	GCAGGGTCAGGGTCAGGGTC	11: 6,599,750 (GRCm38)		probably benign	Het
Nacad	T	C	11: 6,601,622 (GRCm38)	N523S	probably benign	Het
Nacad	A	G	11: 6,601,632 (GRCm38)	C520R	probably benign	Het
Nefh	G	A	11: 4,940,151 (GRCm38)	P823S	probably benign	Het
Nfrkb	G	C	9: 31,397,083 (GRCm38)	A230P	probably benign	Het
Nlrp4a	A	G	7: 26,449,637 (GRCm38)	E223G	probably damaging	Het
Notch3	T	A	17: 32,146,417 (GRCm38)	Y1107F	probably damaging	Het
Olfr1331	G	T	4: 118,869,303 (GRCm38)	R174M	probably benign	Het
Olfr309	A	T	7: 86,306,284 (GRCm38)	Y276*	probably null	Het
Olfr781	A	G	10: 129,333,445 (GRCm38)	D188G	probably benign	Het
Osm	A	G	11: 4,239,588 (GRCm38)	D124G	probably benign	Het
Plekhm2	TTCCTCCTCCT	TTCCTCCT	4: 141,631,981 (GRCm38)		probably benign	Het
Pomgnt1	C	T	4: 116,137,427 (GRCm38)		probably benign	Het
Spen	A	G	4: 141,474,353 (GRCm38)	V2321A	probably benign	Het
Sytl1	TCTGC	TC	4: 133,256,994 (GRCm38)		probably benign	Het
Tcn2	G	C	11: 3,923,487 (GRCm38)	F286L	possibly damaging	Het
Tmprss7	C	T	16: 45,680,733 (GRCm38)	R235Q	probably benign	Het
Tns3	G	T	11: 8,451,146 (GRCm38)	L1051M	probably benign	Het
Tns3	T	G	11: 8,479,518 (GRCm38)	E806A	probably benign	Het
Tns3	G	A	11: 8,549,100 (GRCm38)		probably benign	Het
Toe1	T	C	4: 116,806,093 (GRCm38)	I62M	probably benign	Het
Txnrd2	A	G	16: 18,475,565 (GRCm38)	H436R	probably damaging	Het
Ubr4	C	T	4: 139,451,781 (GRCm38)	P2001S	probably damaging	Het
Vmn2r23	T	A	6: 123,713,161 (GRCm38)	M332K	probably benign	Het
Zbtb8a	T	C	4: 129,360,212 (GRCm38)	H163R	probably benign	Het
Zbtb8a	GG	GGATG	4: 129,360,019 (GRCm38)		probably benign	Het
Zfyve21	A	G	12: 111,827,633 (GRCm38)	D206G	probably damaging	Het
Zkscan4	AGAGGAG	AGAG	13: 21,479,200 (GRCm38)		probably benign	Het
Zmym1	A	C	4: 127,049,673 (GRCm38)	H307Q	probably benign	Het
Zmym1	C	T	4: 127,048,250 (GRCm38)	V684I	probably benign	Het
Zmym1	C	T	4: 127,047,947 (GRCm38)	D785N	probably benign	Het

Other mutations in Tg

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Tg	APN	15	66,847,166 (GRCm38)	missense	probably damaging	1.00
IGL00230:Tg	APN	15	66,827,290 (GRCm38)	missense	probably benign	0.00
IGL00324:Tg	APN	15	66,693,424 (GRCm38)	missense	probably benign
IGL00428:Tg	APN	15	66,773,424 (GRCm38)	missense	probably benign	0.33
IGL00703:Tg	APN	15	66,696,489 (GRCm38)	missense	probably benign	0.34
IGL00808:Tg	APN	15	66,683,813 (GRCm38)	missense	probably damaging	1.00
IGL00833:Tg	APN	15	66,688,801 (GRCm38)	missense	probably benign	0.34
IGL00899:Tg	APN	15	66,674,073 (GRCm38)	critical splice donor site	probably null
IGL00921:Tg	APN	15	66,764,453 (GRCm38)	missense	probably benign	0.28
IGL00975:Tg	APN	15	66,681,882 (GRCm38)	missense	probably benign
IGL01288:Tg	APN	15	66,736,276 (GRCm38)	missense	possibly damaging	0.81
IGL01397:Tg	APN	15	66,696,092 (GRCm38)	splice site	probably benign
IGL01634:Tg	APN	15	66,729,566 (GRCm38)	missense	probably benign	0.34
IGL01646:Tg	APN	15	66,678,087 (GRCm38)	missense	probably damaging	1.00
IGL01704:Tg	APN	15	66,671,351 (GRCm38)	missense	probably damaging	0.98
IGL01958:Tg	APN	15	66,759,486 (GRCm38)	missense	probably benign	0.06
IGL02093:Tg	APN	15	66,692,374 (GRCm38)	missense	possibly damaging	0.83
IGL02113:Tg	APN	15	66,705,330 (GRCm38)	missense	probably benign	0.08
IGL02138:Tg	APN	15	66,717,233 (GRCm38)	missense	probably benign	0.01
IGL02156:Tg	APN	15	66,705,348 (GRCm38)	missense	probably benign	0.19
IGL02169:Tg	APN	15	66,757,943 (GRCm38)	missense	probably benign	0.04
IGL02342:Tg	APN	15	66,764,291 (GRCm38)	missense	probably benign
IGL02434:Tg	APN	15	66,764,342 (GRCm38)	missense	probably damaging	0.97
IGL02506:Tg	APN	15	66,741,594 (GRCm38)	missense	possibly damaging	0.71
IGL02513:Tg	APN	15	66,705,274 (GRCm38)	missense	probably benign
IGL02549:Tg	APN	15	66,839,361 (GRCm38)	missense	probably damaging	1.00
IGL02669:Tg	APN	15	66,748,726 (GRCm38)	splice site	probably benign
IGL02756:Tg	APN	15	66,734,586 (GRCm38)	missense	probably benign
IGL02800:Tg	APN	15	66,757,886 (GRCm38)	missense	probably damaging	1.00
IGL02828:Tg	APN	15	66,682,394 (GRCm38)	missense	probably damaging	1.00
IGL02927:Tg	APN	15	66,678,093 (GRCm38)	missense	probably damaging	1.00
IGL03061:Tg	APN	15	66,671,405 (GRCm38)	missense	probably damaging	1.00
IGL03105:Tg	APN	15	66,715,106 (GRCm38)	missense	probably benign	0.01
IGL03160:Tg	APN	15	66,839,303 (GRCm38)	nonsense	probably null
IGL03242:Tg	APN	15	66,683,798 (GRCm38)	missense	probably damaging	0.99
Also_ran	UTSW	15	66,678,839 (GRCm38)	missense	probably damaging	1.00
bedraggled	UTSW	15	66,740,714 (GRCm38)	missense	probably damaging	1.00
foster	UTSW	15	66,693,260 (GRCm38)	nonsense	probably null
hognose	UTSW	15	66,717,208 (GRCm38)	missense	probably damaging	0.99
ito	UTSW	15	66,766,162 (GRCm38)	nonsense	probably null
ito2	UTSW	15	66,671,396 (GRCm38)	missense	probably damaging	1.00
ito3	UTSW	15	66,773,474 (GRCm38)	missense	probably damaging	1.00
ito4	UTSW	15	66,696,520 (GRCm38)	missense	possibly damaging	0.47
Papua	UTSW	15	66,674,050 (GRCm38)	missense	probably damaging	1.00
Pipistrella	UTSW	15	66,696,135 (GRCm38)	missense	probably damaging	1.00
pluribus	UTSW	15	66,715,163 (GRCm38)	missense	probably damaging	0.98
samarai	UTSW	15	66,758,006 (GRCm38)	critical splice donor site	probably null
sariba	UTSW	15	66,694,870 (GRCm38)	missense	probably benign	0.01
ticker	UTSW	15	66,827,382 (GRCm38)	nonsense	probably null
Vampire	UTSW	15	66,682,827 (GRCm38)	missense	probably damaging	1.00
IGL03134:Tg	UTSW	15	66,740,718 (GRCm38)	missense	probably damaging	1.00
P0019:Tg	UTSW	15	66,688,863 (GRCm38)	missense	probably benign	0.01
R0121:Tg	UTSW	15	66,740,781 (GRCm38)	missense	probably benign	0.04
R0135:Tg	UTSW	15	66,694,870 (GRCm38)	missense	probably benign	0.01
R0227:Tg	UTSW	15	66,698,446 (GRCm38)	missense	possibly damaging	0.84
R0448:Tg	UTSW	15	66,764,442 (GRCm38)	missense	probably damaging	1.00
R0453:Tg	UTSW	15	66,828,533 (GRCm38)	missense	probably benign	0.09
R0504:Tg	UTSW	15	66,682,404 (GRCm38)	missense	probably damaging	0.97
R0543:Tg	UTSW	15	66,729,597 (GRCm38)	missense	probably benign	0.13
R0638:Tg	UTSW	15	66,717,208 (GRCm38)	missense	probably damaging	0.99
R0639:Tg	UTSW	15	66,741,484 (GRCm38)	critical splice acceptor site	probably null
R0646:Tg	UTSW	15	66,729,626 (GRCm38)	missense	probably damaging	0.99
R0666:Tg	UTSW	15	66,737,521 (GRCm38)	missense	probably benign
R0673:Tg	UTSW	15	66,741,484 (GRCm38)	critical splice acceptor site	probably null
R0689:Tg	UTSW	15	66,839,404 (GRCm38)	splice site	probably benign
R0704:Tg	UTSW	15	66,757,880 (GRCm38)	missense	probably benign	0.02
R0730:Tg	UTSW	15	66,678,789 (GRCm38)	missense	probably damaging	1.00
R0830:Tg	UTSW	15	66,725,144 (GRCm38)	missense	probably damaging	1.00
R0959:Tg	UTSW	15	66,708,010 (GRCm38)	missense	probably damaging	0.98
R1027:Tg	UTSW	15	66,672,409 (GRCm38)	missense	possibly damaging	0.65
R1061:Tg	UTSW	15	66,698,559 (GRCm38)	missense	probably benign	0.09
R1086:Tg	UTSW	15	66,684,062 (GRCm38)	missense	probably benign
R1103:Tg	UTSW	15	66,719,655 (GRCm38)	missense	probably benign	0.45
R1240:Tg	UTSW	15	66,828,548 (GRCm38)	missense	probably benign	0.16
R1281:Tg	UTSW	15	66,696,489 (GRCm38)	missense	probably benign	0.34
R1470:Tg	UTSW	15	66,849,463 (GRCm38)	missense	possibly damaging	0.95
R1470:Tg	UTSW	15	66,849,463 (GRCm38)	missense	possibly damaging	0.95
R1531:Tg	UTSW	15	66,850,502 (GRCm38)	missense	probably benign	0.02
R1544:Tg	UTSW	15	66,705,232 (GRCm38)	missense	probably benign	0.04
R1550:Tg	UTSW	15	66,693,430 (GRCm38)	missense	possibly damaging	0.52
R1575:Tg	UTSW	15	66,729,685 (GRCm38)	critical splice donor site	probably null
R1638:Tg	UTSW	15	66,696,166 (GRCm38)	nonsense	probably null
R1655:Tg	UTSW	15	66,828,568 (GRCm38)	critical splice donor site	probably null
R1671:Tg	UTSW	15	66,692,387 (GRCm38)	missense	possibly damaging	0.89
R1789:Tg	UTSW	15	66,737,548 (GRCm38)	missense	probably benign	0.00
R1883:Tg	UTSW	15	66,671,309 (GRCm38)	missense	probably damaging	1.00
R1984:Tg	UTSW	15	66,682,842 (GRCm38)	missense	probably benign
R2063:Tg	UTSW	15	66,828,553 (GRCm38)	missense	probably damaging	1.00
R2092:Tg	UTSW	15	66,849,607 (GRCm38)	missense	probably null	0.26
R2109:Tg	UTSW	15	66,729,594 (GRCm38)	missense	probably benign	0.02
R2128:Tg	UTSW	15	66,694,894 (GRCm38)	missense	probably benign	0.10
R2129:Tg	UTSW	15	66,694,894 (GRCm38)	missense	probably benign	0.10
R2207:Tg	UTSW	15	66,681,939 (GRCm38)	missense	probably benign	0.15
R2219:Tg	UTSW	15	66,681,933 (GRCm38)	missense	probably benign	0.03
R2228:Tg	UTSW	15	66,674,011 (GRCm38)	missense	probably damaging	0.99
R2229:Tg	UTSW	15	66,674,011 (GRCm38)	missense	probably damaging	0.99
R2259:Tg	UTSW	15	66,683,898 (GRCm38)	missense	probably benign
R2994:Tg	UTSW	15	66,681,953 (GRCm38)	missense	probably benign
R3904:Tg	UTSW	15	66,766,162 (GRCm38)	nonsense	probably null
R3946:Tg	UTSW	15	66,674,023 (GRCm38)	missense	probably damaging	1.00
R3965:Tg	UTSW	15	66,684,190 (GRCm38)	missense	probably benign
R4245:Tg	UTSW	15	66,696,469 (GRCm38)	missense	possibly damaging	0.68
R4451:Tg	UTSW	15	66,766,147 (GRCm38)	missense	probably benign	0.01
R4487:Tg	UTSW	15	66,671,396 (GRCm38)	missense	probably damaging	1.00
R4489:Tg	UTSW	15	66,707,942 (GRCm38)	missense	probably damaging	1.00
R4623:Tg	UTSW	15	66,735,271 (GRCm38)	missense	probably benign	0.23
R4659:Tg	UTSW	15	66,673,920 (GRCm38)	missense	possibly damaging	0.67
R4728:Tg	UTSW	15	66,682,827 (GRCm38)	missense	probably damaging	1.00
R4760:Tg	UTSW	15	66,693,319 (GRCm38)	missense	probably damaging	1.00
R4797:Tg	UTSW	15	66,758,006 (GRCm38)	critical splice donor site	probably null
R4944:Tg	UTSW	15	66,764,337 (GRCm38)	missense	probably damaging	1.00
R4998:Tg	UTSW	15	66,674,050 (GRCm38)	missense	probably damaging	1.00
R5009:Tg	UTSW	15	66,696,586 (GRCm38)	missense	probably benign	0.01
R5025:Tg	UTSW	15	66,707,930 (GRCm38)	missense	probably damaging	1.00
R5035:Tg	UTSW	15	66,681,813 (GRCm38)	splice site	probably null
R5049:Tg	UTSW	15	66,827,382 (GRCm38)	nonsense	probably null
R5073:Tg	UTSW	15	66,735,252 (GRCm38)	missense	probably benign	0.05
R5169:Tg	UTSW	15	66,678,780 (GRCm38)	nonsense	probably null
R5185:Tg	UTSW	15	66,773,474 (GRCm38)	missense	probably damaging	1.00
R5227:Tg	UTSW	15	66,759,567 (GRCm38)	missense	possibly damaging	0.87
R5300:Tg	UTSW	15	66,678,855 (GRCm38)	missense	probably damaging	1.00
R5334:Tg	UTSW	15	66,678,055 (GRCm38)	missense	probably damaging	1.00
R5339:Tg	UTSW	15	66,678,093 (GRCm38)	missense	probably damaging	1.00
R5402:Tg	UTSW	15	66,739,168 (GRCm38)	missense	probably damaging	0.98
R5441:Tg	UTSW	15	66,696,520 (GRCm38)	missense	possibly damaging	0.47
R5509:Tg	UTSW	15	66,827,293 (GRCm38)	missense	probably benign	0.45
R5580:Tg	UTSW	15	66,685,300 (GRCm38)	missense	possibly damaging	0.66
R5582:Tg	UTSW	15	66,693,435 (GRCm38)	missense	probably damaging	1.00
R5624:Tg	UTSW	15	66,838,057 (GRCm38)	missense	probably benign	0.11
R5686:Tg	UTSW	15	66,688,889 (GRCm38)	missense	probably benign	0.28
R6042:Tg	UTSW	15	66,683,993 (GRCm38)	missense	probably benign	0.01
R6122:Tg	UTSW	15	66,828,457 (GRCm38)	missense	probably damaging	1.00
R6146:Tg	UTSW	15	66,673,367 (GRCm38)	splice site	probably null
R6159:Tg	UTSW	15	66,735,247 (GRCm38)	missense	possibly damaging	0.71
R6223:Tg	UTSW	15	66,707,922 (GRCm38)	missense	probably benign	0.15
R6480:Tg	UTSW	15	66,671,311 (GRCm38)	missense	probably damaging	1.00
R6505:Tg	UTSW	15	66,759,558 (GRCm38)	missense	probably damaging	0.99
R6531:Tg	UTSW	15	66,839,362 (GRCm38)	missense	probably damaging	0.99
R6614:Tg	UTSW	15	66,735,259 (GRCm38)	missense	probably damaging	0.99
R6698:Tg	UTSW	15	66,839,362 (GRCm38)	missense	probably damaging	1.00
R6798:Tg	UTSW	15	66,678,839 (GRCm38)	missense	probably damaging	1.00
R6837:Tg	UTSW	15	66,696,135 (GRCm38)	missense	probably damaging	1.00
R6861:Tg	UTSW	15	66,688,891 (GRCm38)	missense	probably benign	0.00
R6888:Tg	UTSW	15	66,696,246 (GRCm38)	missense	probably damaging	0.99
R6933:Tg	UTSW	15	66,764,309 (GRCm38)	missense	possibly damaging	0.73
R6983:Tg	UTSW	15	66,693,358 (GRCm38)	missense	probably benign	0.01
R7078:Tg	UTSW	15	66,673,543 (GRCm38)	missense	probably damaging	1.00
R7244:Tg	UTSW	15	66,740,714 (GRCm38)	missense	probably damaging	1.00
R7320:Tg	UTSW	15	66,694,784 (GRCm38)	missense	possibly damaging	0.71
R7334:Tg	UTSW	15	66,725,272 (GRCm38)	missense	probably benign	0.01
R7418:Tg	UTSW	15	66,696,583 (GRCm38)	missense	probably damaging	0.99
R7485:Tg	UTSW	15	66,696,588 (GRCm38)	missense	probably benign	0.04
R7524:Tg	UTSW	15	66,696,161 (GRCm38)	missense	probably benign	0.01
R7529:Tg	UTSW	15	66,694,768 (GRCm38)	missense	probably damaging	0.99
R7540:Tg	UTSW	15	66,689,927 (GRCm38)	missense	probably benign	0.16
R7583:Tg	UTSW	15	66,764,418 (GRCm38)	missense	probably damaging	1.00
R7594:Tg	UTSW	15	66,729,583 (GRCm38)	missense	probably benign	0.20
R7667:Tg	UTSW	15	66,715,163 (GRCm38)	missense	probably damaging	0.98
R7722:Tg	UTSW	15	66,764,309 (GRCm38)	missense	possibly damaging	0.73
R7790:Tg	UTSW	15	66,849,604 (GRCm38)	missense	probably damaging	0.99
R7838:Tg	UTSW	15	66,693,263 (GRCm38)	missense	probably benign	0.00
R7890:Tg	UTSW	15	66,683,814 (GRCm38)	missense	probably damaging	1.00
R7904:Tg	UTSW	15	66,705,279 (GRCm38)	missense	probably benign	0.08
R7919:Tg	UTSW	15	66,684,074 (GRCm38)	missense	possibly damaging	0.73
R7921:Tg	UTSW	15	66,683,793 (GRCm38)	missense	probably benign	0.08
R8037:Tg	UTSW	15	66,688,875 (GRCm38)	missense	probably benign	0.00
R8038:Tg	UTSW	15	66,688,875 (GRCm38)	missense	probably benign	0.00
R8214:Tg	UTSW	15	66,773,398 (GRCm38)	missense	probably damaging	1.00
R8304:Tg	UTSW	15	66,693,260 (GRCm38)	nonsense	probably null
R8688:Tg	UTSW	15	66,694,953 (GRCm38)	critical splice donor site	probably benign
R8709:Tg	UTSW	15	66,681,937 (GRCm38)	missense	probably benign	0.08
R8714:Tg	UTSW	15	66,684,042 (GRCm38)	missense	probably damaging	0.97
R8901:Tg	UTSW	15	66,685,335 (GRCm38)	missense	probably damaging	1.00
R8917:Tg	UTSW	15	66,773,483 (GRCm38)	critical splice donor site	probably null
R9023:Tg	UTSW	15	66,683,673 (GRCm38)	missense	probably damaging	1.00
R9232:Tg	UTSW	15	66,698,461 (GRCm38)	missense	probably benign	0.01
R9310:Tg	UTSW	15	66,827,269 (GRCm38)	missense	possibly damaging	0.69
R9361:Tg	UTSW	15	66,685,397 (GRCm38)	missense	possibly damaging	0.50
R9389:Tg	UTSW	15	66,689,324 (GRCm38)	missense	probably benign	0.04
R9501:Tg	UTSW	15	66,847,074 (GRCm38)	missense	possibly damaging	0.52
R9510:Tg	UTSW	15	66,674,064 (GRCm38)	missense	probably damaging	1.00
R9594:Tg	UTSW	15	66,735,260 (GRCm38)	nonsense	probably null
R9629:Tg	UTSW	15	66,683,738 (GRCm38)	missense	possibly damaging	0.95
R9701:Tg	UTSW	15	66,766,142 (GRCm38)	missense	probably benign	0.03
R9743:Tg	UTSW	15	66,689,990 (GRCm38)	missense	probably benign	0.18
R9748:Tg	UTSW	15	66,847,159 (GRCm38)	missense	possibly damaging	0.91
X0005:Tg	UTSW	15	66,688,863 (GRCm38)	missense	probably benign	0.01
X0065:Tg	UTSW	15	66,682,454 (GRCm38)	missense	probably damaging	1.00
X0067:Tg	UTSW	15	66,748,743 (GRCm38)	missense	probably benign	0.10
Z1177:Tg	UTSW	15	66,849,547 (GRCm38)	missense	probably benign	0.02
Z1177:Tg	UTSW	15	66,685,310 (GRCm38)	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- TGTCTACCCGAGAGGAACACTTGTC -3'
(R):5'- TGAAGGCCCCTGCTTCCATAACAC -3'

Sequencing Primer
(F):5'- CACTTGTCAGGTTATAAGAAGGCTG -3'
(R):5'- TGCTTCCATAACACAGGGG -3'

Posted On

2013-09-03