Mutagenetix > Incidental Mutation

Incidental Mutation 'R8886:Greb1l'

677359

Institutional Source

Beutler Lab

Gene Symbol

Greb1l

Ensembl Gene

ENSMUSG00000042942

Gene Name

growth regulation by estrogen in breast cancer-like

Synonyms

AK220484, mKIAA4095

MMRRC Submission

068691-MU

Accession Numbers

Genbank: NM_001083628; MGI: 3576497

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8886 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

10325177-10562934 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 10553843 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 1615 (I1615F)

Ref Sequence

ENSEMBL: ENSMUSP00000049003 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048977]

AlphaFold

B9EJV3

Predicted Effect

probably benign
Transcript: ENSMUST00000048977
AA Change: I1615F

PolyPhen 2 Score 0.209 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000049003
Gene: ENSMUSG00000042942
AA Change: I1615F

Domain	Start	End	E-Value	Type
Pfam:GREB1	1	1172	N/A	PFAM
Pfam:GREB1	1154	1913	N/A	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

99% (72/73)

Allele List at MGI

All alleles(5) : Targeted, other(2) Gene trapped(3)

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700025G04Rik	T	A	1: 152,006,598 (GRCm38)	N14I	possibly damaging	Het
1700066M21Rik	T	A	1: 57,382,849 (GRCm38)	I128N	probably damaging	Het
4930407I10Rik	G	T	15: 82,065,850 (GRCm38)	S1316I	probably damaging	Het
Abcc9	T	C	6: 142,600,694 (GRCm38)		probably benign	Het
Adgrb3	A	G	1: 25,111,847 (GRCm38)	S1102P	probably damaging	Het
BC061237	A	C	14: 44,504,234 (GRCm38)	I145L	probably benign	Het
Birc7	A	T	2: 180,932,993 (GRCm38)		probably benign	Het
Boc	C	T	16: 44,499,443 (GRCm38)	G393S		Het
Ccdc112	C	T	18: 46,311,759 (GRCm38)	G18R	unknown	Het
Cryzl1	T	A	16: 91,695,300 (GRCm38)	T213S	possibly damaging	Het
Cttnbp2	T	C	6: 18,414,299 (GRCm38)	N916D	probably benign	Het
Cyp4v3	A	T	8: 45,321,748 (GRCm38)	L123*	probably null	Het
Dmpk	C	A	7: 19,091,961 (GRCm38)		probably benign	Het
Dnah9	A	T	11: 66,053,014 (GRCm38)	V1932E	probably damaging	Het
Ect2	T	C	3: 27,145,977 (GRCm38)		probably benign	Het
Eif4a3	A	G	11: 119,288,879 (GRCm38)	Y361H	probably damaging	Het
Fkbp10	A	G	11: 100,422,036 (GRCm38)	E336G	probably damaging	Het
Gnptg	T	C	17: 25,234,654 (GRCm38)	S305G	probably benign	Het
Gse1	G	A	8: 120,570,731 (GRCm38)	R561Q	unknown	Het
Igkv9-129	T	A	6: 67,840,237 (GRCm38)	Y108*	probably null	Het
Igsf10	T	A	3: 59,329,989 (GRCm38)	T924S	probably benign	Het
Itgb6	A	T	2: 60,627,980 (GRCm38)	C476*	probably null	Het
Itih4	C	T	14: 30,895,525 (GRCm38)	Q601*	probably null	Het
Itpk1	G	A	12: 102,584,345 (GRCm38)		probably benign	Het
Itpr3	G	A	17: 27,118,677 (GRCm38)		probably benign	Het
Jakmip3	G	A	7: 139,007,442 (GRCm38)	E92K	probably benign	Het
Klhl29	A	T	12: 5,137,542 (GRCm38)	L274H	possibly damaging	Het
Klk1b5	T	A	7: 44,219,768 (GRCm38)	M122K	probably damaging	Het
Krt84	A	G	15: 101,528,786 (GRCm38)	I314T	possibly damaging	Het
Lama2	A	T	10: 27,369,161 (GRCm38)		probably benign	Het
Lmntd1	T	C	6: 145,417,426 (GRCm38)	T270A	probably damaging	Het
Mindy4	T	C	6: 55,278,238 (GRCm38)	L567P	probably benign	Het
Mt2	A	T	8: 94,172,848 (GRCm38)	M1L	probably damaging	Het
Myct1	C	T	10: 5,604,208 (GRCm38)	T25I	probably damaging	Het
Myh11	A	G	16: 14,234,414 (GRCm38)	I396T		Het
Naprt	T	C	15: 75,893,584 (GRCm38)	T136A	probably damaging	Het
Nbea	T	C	3: 56,058,727 (GRCm38)	N438S	probably damaging	Het
Nckap1	C	A	2: 80,508,711 (GRCm38)		probably null	Het
Nop56	G	T	2: 130,275,982 (GRCm38)	R126L	probably damaging	Het
Nts	C	T	10: 102,485,007 (GRCm38)	A74T	probably benign	Het
Ogfr	GGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCCAAAGCCAGGTGG	GGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCCAAAGCCAGGTGG	2: 180,595,266 (GRCm38)		probably benign	Het
Olfr913	T	G	9: 38,595,150 (GRCm38)	F310V	possibly damaging	Het
Pcp2	A	G	8: 3,625,208 (GRCm38)		probably null	Het
Pdgfra	T	C	5: 75,183,073 (GRCm38)	S752P	probably benign	Het
Pign	C	T	1: 105,585,054 (GRCm38)	V635I	probably benign	Het
Plekhg3	C	A	12: 76,564,974 (GRCm38)	H342Q	possibly damaging	Het
Ppp4c	A	T	7: 126,787,294 (GRCm38)	I177N	probably damaging	Het
Psmd5	A	G	2: 34,857,743 (GRCm38)	F301L	possibly damaging	Het
Rad52	C	T	6: 119,913,080 (GRCm38)	R56C	probably damaging	Het
Rbm20	A	T	19: 53,813,336 (GRCm38)	I92L	probably benign	Het
Rnf213	A	G	11: 119,473,438 (GRCm38)	D4522G		Het
Robo3	T	C	9: 37,417,472 (GRCm38)	E1276G	probably damaging	Het
Rxrb	T	C	17: 34,037,454 (GRCm38)	F500L	probably damaging	Het
Sar1a	A	T	10: 61,686,393 (GRCm38)	N88I	possibly damaging	Het
Scd3	A	G	19: 44,241,837 (GRCm38)	D353G	probably damaging	Het
Sh2d4b	T	C	14: 40,873,989 (GRCm38)		probably benign	Het
Ski	A	T	4: 155,159,559 (GRCm38)	L498Q	probably null	Het
Spocd1	T	A	4: 129,951,838 (GRCm38)	M497K		Het
Sspo	A	G	6: 48,481,267 (GRCm38)	E3299G	possibly damaging	Het
St14	C	A	9: 31,097,124 (GRCm38)	V507F	possibly damaging	Het
Tcf24	G	A	1: 9,967,163 (GRCm38)	T108I	probably benign	Het
Tecta	T	C	9: 42,367,063 (GRCm38)	T1050A	probably benign	Het
Ttc17	T	C	2: 94,375,128 (GRCm38)	D291G	probably benign	Het
Tubgcp2	G	T	7: 140,004,969 (GRCm38)	R518S	probably benign	Het
Ugt2a3	T	C	5: 87,336,499 (GRCm38)	Y222C	probably damaging	Het
Unc5c	C	G	3: 141,803,820 (GRCm38)	A687G	probably benign	Het
Usp42	T	C	5: 143,714,959 (GRCm38)	D1103G	probably damaging	Het
Vmn1r83	A	C	7: 12,321,916 (GRCm38)	D71E	probably benign	Het
Vmn2r3	T	A	3: 64,287,471 (GRCm38)	I9F	possibly damaging	Het
Zfand1	T	C	3: 10,344,802 (GRCm38)	Q160R	probably null	Het
Zhx3	T	C	2: 160,781,296 (GRCm38)	N317S	probably damaging	Het

Other mutations in Greb1l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00485:Greb1l	APN	18	10,555,962 (GRCm38)	missense	possibly damaging	0.90
IGL01554:Greb1l	APN	18	10,522,144 (GRCm38)	missense	probably benign	0.01
IGL01563:Greb1l	APN	18	10,469,399 (GRCm38)	missense	probably damaging	0.99
IGL01944:Greb1l	APN	18	10,557,280 (GRCm38)	missense	possibly damaging	0.91
IGL02110:Greb1l	APN	18	10,515,271 (GRCm38)	missense	probably damaging	1.00
IGL02249:Greb1l	APN	18	10,532,961 (GRCm38)	missense	probably damaging	1.00
IGL02318:Greb1l	APN	18	10,469,388 (GRCm38)	missense	possibly damaging	0.91
IGL02340:Greb1l	APN	18	10,515,200 (GRCm38)	missense	probably damaging	0.99
IGL02516:Greb1l	APN	18	10,537,064 (GRCm38)	missense	probably benign	0.31
IGL02566:Greb1l	APN	18	10,503,299 (GRCm38)	missense	probably damaging	0.99
IGL02583:Greb1l	APN	18	10,542,362 (GRCm38)	missense	probably damaging	1.00
IGL02838:Greb1l	APN	18	10,560,430 (GRCm38)	missense	probably damaging	1.00
A4554:Greb1l	UTSW	18	10,532,862 (GRCm38)	missense	possibly damaging	0.58
PIT4453001:Greb1l	UTSW	18	10,533,032 (GRCm38)	missense	probably benign	0.08
PIT4453001:Greb1l	UTSW	18	10,533,031 (GRCm38)	missense	probably damaging	0.98
R0099:Greb1l	UTSW	18	10,509,158 (GRCm38)	missense	probably damaging	1.00
R0226:Greb1l	UTSW	18	10,522,076 (GRCm38)	intron	probably benign
R0234:Greb1l	UTSW	18	10,560,331 (GRCm38)	missense	probably damaging	1.00
R0234:Greb1l	UTSW	18	10,560,331 (GRCm38)	missense	probably damaging	1.00
R0239:Greb1l	UTSW	18	10,458,567 (GRCm38)	splice site	probably benign
R0316:Greb1l	UTSW	18	10,547,420 (GRCm38)	missense	probably damaging	1.00
R0369:Greb1l	UTSW	18	10,469,375 (GRCm38)	missense	possibly damaging	0.80
R0394:Greb1l	UTSW	18	10,523,374 (GRCm38)	missense	probably damaging	0.99
R0478:Greb1l	UTSW	18	10,509,281 (GRCm38)	missense	probably damaging	1.00
R0555:Greb1l	UTSW	18	10,458,781 (GRCm38)	splice site	probably benign
R0671:Greb1l	UTSW	18	10,474,303 (GRCm38)	missense	probably damaging	1.00
R1282:Greb1l	UTSW	18	10,547,289 (GRCm38)	missense	probably benign	0.13
R1574:Greb1l	UTSW	18	10,554,997 (GRCm38)	missense	possibly damaging	0.95
R1574:Greb1l	UTSW	18	10,554,997 (GRCm38)	missense	possibly damaging	0.95
R1607:Greb1l	UTSW	18	10,529,703 (GRCm38)	missense	possibly damaging	0.85
R1666:Greb1l	UTSW	18	10,529,708 (GRCm38)	critical splice donor site	probably null
R1666:Greb1l	UTSW	18	10,501,080 (GRCm38)	critical splice donor site	probably null
R1720:Greb1l	UTSW	18	10,553,848 (GRCm38)	missense	probably benign	0.19
R1808:Greb1l	UTSW	18	10,542,143 (GRCm38)	missense	probably benign
R1829:Greb1l	UTSW	18	10,509,314 (GRCm38)	missense	probably damaging	1.00
R1897:Greb1l	UTSW	18	10,498,992 (GRCm38)	missense	probably benign	0.00
R1967:Greb1l	UTSW	18	10,501,049 (GRCm38)	missense	possibly damaging	0.91
R2025:Greb1l	UTSW	18	10,515,221 (GRCm38)	missense	possibly damaging	0.71
R2086:Greb1l	UTSW	18	10,523,281 (GRCm38)	missense	probably damaging	1.00
R2125:Greb1l	UTSW	18	10,511,422 (GRCm38)	missense	probably damaging	0.98
R2139:Greb1l	UTSW	18	10,555,011 (GRCm38)	missense	probably damaging	1.00
R2255:Greb1l	UTSW	18	10,554,857 (GRCm38)	missense	probably damaging	1.00
R2256:Greb1l	UTSW	18	10,503,307 (GRCm38)	missense	possibly damaging	0.91
R2257:Greb1l	UTSW	18	10,503,307 (GRCm38)	missense	possibly damaging	0.91
R2880:Greb1l	UTSW	18	10,547,288 (GRCm38)	missense	possibly damaging	0.93
R3623:Greb1l	UTSW	18	10,542,380 (GRCm38)	missense	probably damaging	0.99
R3778:Greb1l	UTSW	18	10,469,444 (GRCm38)	missense	possibly damaging	0.60
R3975:Greb1l	UTSW	18	10,522,247 (GRCm38)	missense	possibly damaging	0.71
R4038:Greb1l	UTSW	18	10,515,209 (GRCm38)	missense	possibly damaging	0.93
R4062:Greb1l	UTSW	18	10,522,150 (GRCm38)	missense	probably damaging	0.99
R4134:Greb1l	UTSW	18	10,529,708 (GRCm38)	critical splice donor site	probably null
R4342:Greb1l	UTSW	18	10,544,561 (GRCm38)	missense	probably benign	0.12
R4409:Greb1l	UTSW	18	10,503,182 (GRCm38)	missense	possibly damaging	0.70
R4600:Greb1l	UTSW	18	10,553,705 (GRCm38)	missense	probably damaging	1.00
R4618:Greb1l	UTSW	18	10,498,965 (GRCm38)	missense	probably benign	0.00
R4683:Greb1l	UTSW	18	10,529,563 (GRCm38)	splice site	probably null
R4686:Greb1l	UTSW	18	10,522,112 (GRCm38)	missense	probably damaging	0.98
R4707:Greb1l	UTSW	18	10,532,922 (GRCm38)	missense	probably benign	0.02
R4780:Greb1l	UTSW	18	10,541,792 (GRCm38)	missense	probably benign	0.00
R4819:Greb1l	UTSW	18	10,458,358 (GRCm38)	missense	probably damaging	1.00
R4925:Greb1l	UTSW	18	10,547,447 (GRCm38)	missense	possibly damaging	0.79
R4960:Greb1l	UTSW	18	10,547,306 (GRCm38)	missense	probably damaging	0.99
R5150:Greb1l	UTSW	18	10,555,950 (GRCm38)	frame shift	probably null
R5154:Greb1l	UTSW	18	10,458,312 (GRCm38)	missense	probably benign	0.02
R5269:Greb1l	UTSW	18	10,511,409 (GRCm38)	missense	probably benign
R5290:Greb1l	UTSW	18	10,542,427 (GRCm38)	missense	probably damaging	1.00
R5310:Greb1l	UTSW	18	10,542,427 (GRCm38)	missense	probably damaging	1.00
R5328:Greb1l	UTSW	18	10,553,720 (GRCm38)	missense	probably damaging	1.00
R5337:Greb1l	UTSW	18	10,509,143 (GRCm38)	missense	probably damaging	1.00
R5393:Greb1l	UTSW	18	10,458,312 (GRCm38)	missense	probably benign	0.02
R5402:Greb1l	UTSW	18	10,537,169 (GRCm38)	missense	probably benign	0.26
R5718:Greb1l	UTSW	18	10,542,427 (GRCm38)	missense	probably damaging	1.00
R5719:Greb1l	UTSW	18	10,542,427 (GRCm38)	missense	probably damaging	1.00
R5720:Greb1l	UTSW	18	10,542,427 (GRCm38)	missense	probably damaging	1.00
R5721:Greb1l	UTSW	18	10,542,427 (GRCm38)	missense	probably damaging	1.00
R5902:Greb1l	UTSW	18	10,538,302 (GRCm38)	missense	probably benign	0.00
R5993:Greb1l	UTSW	18	10,544,455 (GRCm38)	missense	probably benign	0.10
R6035:Greb1l	UTSW	18	10,501,025 (GRCm38)	missense	possibly damaging	0.91
R6035:Greb1l	UTSW	18	10,501,025 (GRCm38)	missense	possibly damaging	0.91
R6045:Greb1l	UTSW	18	10,547,068 (GRCm38)	missense	probably damaging	1.00
R6063:Greb1l	UTSW	18	10,557,340 (GRCm38)	missense	probably damaging	1.00
R6297:Greb1l	UTSW	18	10,469,494 (GRCm38)	missense	probably damaging	1.00
R6405:Greb1l	UTSW	18	10,501,076 (GRCm38)	missense	probably benign	0.30
R6552:Greb1l	UTSW	18	10,541,814 (GRCm38)	missense	probably benign	0.00
R6572:Greb1l	UTSW	18	10,522,131 (GRCm38)	missense	probably benign	0.07
R6575:Greb1l	UTSW	18	10,547,347 (GRCm38)	missense	possibly damaging	0.88
R6922:Greb1l	UTSW	18	10,547,482 (GRCm38)	missense	possibly damaging	0.88
R6957:Greb1l	UTSW	18	10,558,786 (GRCm38)	missense	probably benign	0.23
R6962:Greb1l	UTSW	18	10,547,327 (GRCm38)	missense	probably damaging	1.00
R7012:Greb1l	UTSW	18	10,529,707 (GRCm38)	critical splice donor site	probably null
R7179:Greb1l	UTSW	18	10,544,576 (GRCm38)	missense	probably benign	0.00
R7251:Greb1l	UTSW	18	10,515,319 (GRCm38)	missense	probably damaging	1.00
R7275:Greb1l	UTSW	18	10,544,561 (GRCm38)	missense	probably benign	0.12
R7301:Greb1l	UTSW	18	10,544,970 (GRCm38)	missense	probably damaging	1.00
R7307:Greb1l	UTSW	18	10,538,142 (GRCm38)	missense	probably damaging	0.99
R7455:Greb1l	UTSW	18	10,554,915 (GRCm38)	missense	probably damaging	1.00
R7832:Greb1l	UTSW	18	10,542,056 (GRCm38)	missense	probably benign	0.38
R7934:Greb1l	UTSW	18	10,474,371 (GRCm38)	nonsense	probably null
R8137:Greb1l	UTSW	18	10,474,357 (GRCm38)	missense	possibly damaging	0.77
R8138:Greb1l	UTSW	18	10,533,060 (GRCm38)	missense	probably benign	0.13
R8208:Greb1l	UTSW	18	10,510,703 (GRCm38)	missense	probably damaging	1.00
R8227:Greb1l	UTSW	18	10,515,371 (GRCm38)	missense	probably damaging	1.00
R8312:Greb1l	UTSW	18	10,511,587 (GRCm38)	intron	probably benign
R8331:Greb1l	UTSW	18	10,458,706 (GRCm38)	missense	possibly damaging	0.96
R8364:Greb1l	UTSW	18	10,529,687 (GRCm38)	missense	possibly damaging	0.85
R8389:Greb1l	UTSW	18	10,529,613 (GRCm38)	missense	probably benign	0.00
R8695:Greb1l	UTSW	18	10,544,450 (GRCm38)	missense	probably benign	0.01
R8795:Greb1l	UTSW	18	10,553,739 (GRCm38)	missense	probably damaging	0.98
R8836:Greb1l	UTSW	18	10,509,257 (GRCm38)	missense	probably benign	0.30
R8862:Greb1l	UTSW	18	10,555,042 (GRCm38)	missense	possibly damaging	0.90
R8872:Greb1l	UTSW	18	10,529,684 (GRCm38)	missense	probably benign	0.18
R8874:Greb1l	UTSW	18	10,544,896 (GRCm38)	missense	probably benign	0.01
R8921:Greb1l	UTSW	18	10,541,825 (GRCm38)	missense	probably benign	0.01
R8997:Greb1l	UTSW	18	10,510,747 (GRCm38)	missense	probably damaging	1.00
R9015:Greb1l	UTSW	18	10,541,675 (GRCm38)	missense	probably benign	0.00
R9018:Greb1l	UTSW	18	10,542,004 (GRCm38)	missense	possibly damaging	0.76
R9074:Greb1l	UTSW	18	10,558,795 (GRCm38)	missense	probably damaging	1.00
R9074:Greb1l	UTSW	18	10,532,797 (GRCm38)	missense	probably damaging	1.00
R9117:Greb1l	UTSW	18	10,542,422 (GRCm38)	missense	probably benign	0.31
R9189:Greb1l	UTSW	18	10,499,983 (GRCm38)	missense	probably benign
R9332:Greb1l	UTSW	18	10,532,796 (GRCm38)	missense	possibly damaging	0.92
R9367:Greb1l	UTSW	18	10,522,130 (GRCm38)	missense	probably benign	0.00
R9497:Greb1l	UTSW	18	10,458,600 (GRCm38)	missense	probably benign	0.00
R9796:Greb1l	UTSW	18	10,538,233 (GRCm38)	missense	possibly damaging	0.69
Z1176:Greb1l	UTSW	18	10,515,305 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGTACCAAACTAGCATCCTCTTC -3'
(R):5'- CGTAACCAGTGAGCTAGTGAG -3'

Sequencing Primer
(F):5'- CCCGTGTAAACAGTATGCCAGTG -3'
(R):5'- AGAGTAGTCTCATGATCCACTTTGC -3'

Posted On

2021-08-02