Mutagenetix > Incidental Mutation

Incidental Mutation 'R8887:Vcan'

677430

Institutional Source

Beutler Lab

Gene Symbol

Vcan

Ensembl Gene

ENSMUSG00000021614

Gene Name

versican

Synonyms

PG-M, hdf, heart defect, Cspg2, DPEAAE, 5430420N07Rik

MMRRC Submission

068752-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8887 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

89803431-89890628 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 89853026 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 645 (T645A)

Ref Sequence

ENSEMBL: ENSMUSP00000105173 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109543] [ENSMUST00000109544] [ENSMUST00000109546] [ENSMUST00000159910]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000109543

SMART Domains

Protein: ENSMUSP00000105170
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
EGF	351	384	2.72e-7	SMART
EGF_CA	386	422	1.16e-10	SMART
CLECT	428	549	3.08e-34	SMART
CCP	555	611	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109544
AA Change: T645A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000105171
Gene: ENSMUSG00000021614
AA Change: T645A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
EGF	1311	1344	2.72e-7	SMART
EGF_CA	1346	1382	1.16e-10	SMART
CLECT	1388	1509	3.08e-34	SMART
CCP	1515	1571	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109546
AA Change: T645A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000105173
Gene: ENSMUSG00000021614
AA Change: T645A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
low complexity region	1322	1333	N/A	INTRINSIC
low complexity region	1546	1569	N/A	INTRINSIC
low complexity region	1837	1852	N/A	INTRINSIC
low complexity region	2013	2026	N/A	INTRINSIC
low complexity region	2354	2367	N/A	INTRINSIC
low complexity region	2468	2482	N/A	INTRINSIC
low complexity region	2719	2728	N/A	INTRINSIC
EGF	3050	3083	2.72e-7	SMART
EGF_CA	3085	3121	1.16e-10	SMART
CLECT	3127	3248	3.08e-34	SMART
CCP	3254	3310	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000159910

SMART Domains

Protein: ENSMUSP00000125446
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	362	373	N/A	INTRINSIC
low complexity region	586	609	N/A	INTRINSIC
low complexity region	877	892	N/A	INTRINSIC
low complexity region	1053	1066	N/A	INTRINSIC
low complexity region	1394	1407	N/A	INTRINSIC
low complexity region	1508	1522	N/A	INTRINSIC
low complexity region	1759	1768	N/A	INTRINSIC
EGF	2090	2123	2.72e-7	SMART
EGF_CA	2125	2161	1.16e-10	SMART
CLECT	2167	2288	3.08e-34	SMART
CCP	2294	2350	1.04e-8	SMART

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.5%

Validation Efficiency

98% (93/95)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
PHENOTYPE: Homozygotes for an insertional mutation exhibit anterior-posterior segmental defects of the heart, lack endocardial cushions of the conus and atrioventricular region, and die and around embryonic day 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 97 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsbg2	T	C	17: 57,175,285 (GRCm39)	T76A	probably benign	Het
Actn1	A	G	12: 80,215,197 (GRCm39)	C860R	probably damaging	Het
Adam23	T	A	1: 63,554,744 (GRCm39)	L170Q	probably damaging	Het
Afdn	C	T	17: 14,116,401 (GRCm39)	R1620*	probably null	Het
Agfg1	A	G	1: 82,848,525 (GRCm39)		probably benign	Het
Aire	A	G	10: 77,870,298 (GRCm39)	S476P	probably damaging	Het
Arhgef26	A	G	3: 62,247,401 (GRCm39)	T162A	probably benign	Het
Atp1a2	T	C	1: 172,113,222 (GRCm39)	Q487R	probably null	Het
BC048507	T	A	13: 68,011,628 (GRCm39)	C2S	probably benign	Het
Bltp1	G	T	3: 37,087,503 (GRCm39)	A615S	possibly damaging	Het
Bnc2	A	C	4: 84,209,707 (GRCm39)		probably benign	Het
Bop1	C	T	15: 76,338,524 (GRCm39)	G473R	probably damaging	Het
C1galt1	A	G	6: 7,866,379 (GRCm39)	E75G	probably benign	Het
Cacna1i	T	C	15: 80,258,894 (GRCm39)	V1201A	possibly damaging	Het
Cecr2	C	A	6: 120,715,162 (GRCm39)	T249K	probably damaging	Het
Clca3a2	C	A	3: 144,790,810 (GRCm39)	G421*	probably null	Het
Clec1b	A	T	6: 129,378,703 (GRCm39)		probably null	Het
Cnksr3	C	T	10: 7,104,467 (GRCm39)	D79N	probably damaging	Het
Col15a1	A	G	4: 47,287,091 (GRCm39)	Q843R	probably damaging	Het
Col6a3	T	C	1: 90,755,948 (GRCm39)	S114G	probably benign	Het
Corin	A	T	5: 72,486,953 (GRCm39)		probably null	Het
Cyp2u1	A	G	3: 131,096,503 (GRCm39)	Y92H	probably damaging	Het
Dcbld2	T	C	16: 58,229,270 (GRCm39)	L51P	probably damaging	Het
Dip2c	T	A	13: 9,673,989 (GRCm39)		probably benign	Het
Dlc1	A	T	8: 37,051,481 (GRCm39)	V299E	probably benign	Het
Dlgap2	G	A	8: 14,229,682 (GRCm39)		probably null	Het
Dnah1	A	G	14: 31,032,997 (GRCm39)	L346P	probably damaging	Het
Dnah9	T	C	11: 65,746,210 (GRCm39)	T3968A	probably benign	Het
Duox2	A	C	2: 122,120,044 (GRCm39)	M822R	probably null	Het
E2f7	A	T	10: 110,610,674 (GRCm39)	Q433L	probably benign	Het
Eif3k	A	C	7: 28,679,901 (GRCm39)	Y42*	probably null	Het
Elmod3	A	G	6: 72,563,494 (GRCm39)	S45P	probably damaging	Het
Emilin3	C	A	2: 160,751,108 (GRCm39)	V214F	possibly damaging	Het
Fbxo10	A	T	4: 45,058,887 (GRCm39)	F283L	probably benign	Het
Fbxw7	T	C	3: 84,876,549 (GRCm39)	Y358H		Het
G430095P16Rik	A	G	8: 85,453,043 (GRCm39)	N10S	unknown	Het
Gldc	A	G	19: 30,111,156 (GRCm39)	V540A	possibly damaging	Het
Gm3667	T	A	14: 18,271,553 (GRCm39)	E67D	probably benign	Het
Golga3	T	A	5: 110,353,626 (GRCm39)		probably benign	Het
H60c	T	C	10: 3,217,255 (GRCm39)	E38G	probably benign	Het
Hectd4	A	G	5: 121,433,541 (GRCm39)	D952G	probably benign	Het
Hoxb8	T	A	11: 96,175,223 (GRCm39)	L220Q	probably damaging	Het
Ice1	A	T	13: 70,751,050 (GRCm39)	L1679M	probably damaging	Het
Ifi202b	T	A	1: 173,802,480 (GRCm39)	Y118F	probably damaging	Het
Ift122	A	T	6: 115,868,880 (GRCm39)	T456S	probably benign	Het
Ighg3	G	A	12: 113,323,845 (GRCm39)	T181I		Het
Ighv1-66	C	A	12: 115,557,032 (GRCm39)	V17F	probably damaging	Het
Ighv5-12-4	A	G	12: 113,726,130 (GRCm39)	V31A	possibly damaging	Het
Ints12	T	A	3: 132,815,003 (GRCm39)	N403K	probably damaging	Het
Itpr3	G	A	17: 27,337,651 (GRCm39)		probably benign	Het
Kalrn	A	C	16: 34,047,496 (GRCm39)	N908K	probably benign	Het
Kctd9	G	A	14: 67,962,016 (GRCm39)	V20I	unknown	Het
Krt78	G	T	15: 101,861,746 (GRCm39)	L167M	probably damaging	Het
Ldb1	T	C	19: 46,023,294 (GRCm39)	E206G	probably damaging	Het
Lig1	A	G	7: 13,030,713 (GRCm39)	Y455C	probably damaging	Het
Ltbp1	T	G	17: 75,486,077 (GRCm39)	I34S	probably damaging	Het
Map2	T	C	1: 66,454,758 (GRCm39)	I1216T	possibly damaging	Het
Mex3c	T	C	18: 73,706,800 (GRCm39)	V229A	probably damaging	Het
Mfsd11	T	C	11: 116,745,526 (GRCm39)		probably null	Het
Mroh1	A	G	15: 76,331,474 (GRCm39)	T1233A	probably benign	Het
Myct1	C	T	10: 5,554,208 (GRCm39)	T25I	probably damaging	Het
Nipbl	A	T	15: 8,391,271 (GRCm39)	H234Q	probably damaging	Het
Npc1l1	G	A	11: 6,175,665 (GRCm39)	P549S	probably damaging	Het
Or12d2	A	T	17: 37,624,642 (GRCm39)	L211Q	probably damaging	Het
Or4c3	A	G	2: 89,852,269 (GRCm39)	V47A	probably benign	Het
Or5h22	A	T	16: 58,894,846 (GRCm39)	V199D	possibly damaging	Het
Or5w22	A	G	2: 87,363,187 (GRCm39)	D270G	possibly damaging	Het
Or6c3b	A	T	10: 129,527,372 (GRCm39)	Y179*	probably null	Het
Padi3	A	T	4: 140,523,795 (GRCm39)	C228*	probably null	Het
Palld	T	A	8: 61,986,512 (GRCm39)	D1015V	unknown	Het
Pcna-ps2	A	T	19: 9,261,488 (GRCm39)	Y249F	probably benign	Het
Pelo	C	T	13: 115,225,451 (GRCm39)	C258Y	probably benign	Het
Ppp1r13b	A	T	12: 111,803,430 (GRCm39)		probably benign	Het
Pramel26	A	G	4: 143,539,257 (GRCm39)	F79L	probably damaging	Het
Prdm2	A	G	4: 142,860,771 (GRCm39)	W840R	probably damaging	Het
Prkcg	A	G	7: 3,370,857 (GRCm39)	D428G	possibly damaging	Het
Pus7	G	A	5: 23,948,476 (GRCm39)	R571*	probably null	Het
Rab11fip3	A	G	17: 26,286,927 (GRCm39)	C409R	possibly damaging	Het
Rdh13	T	C	7: 4,434,522 (GRCm39)	D186G	probably damaging	Het
Rslcan18	T	A	13: 67,246,793 (GRCm39)	H273L	probably damaging	Het
Rxfp1	T	A	3: 79,559,289 (GRCm39)		probably benign	Het
Sh3bp1	T	A	15: 78,788,540 (GRCm39)		probably null	Het
Siglecg	T	A	7: 43,058,008 (GRCm39)	S9T	probably benign	Het
Slc9a1	T	C	4: 133,139,258 (GRCm39)	F159L	probably benign	Het
Slc9a2	A	G	1: 40,758,009 (GRCm39)	I183V	probably benign	Het
St6galnac4	A	G	2: 32,484,110 (GRCm39)	M103V	probably damaging	Het
Tbc1d30	T	C	10: 121,187,059 (GRCm39)	D42G	possibly damaging	Het
Tlr5	C	A	1: 182,801,332 (GRCm39)	T212K	probably benign	Het
Trim43b	T	G	9: 88,969,642 (GRCm39)	I269L	probably benign	Het
Ttll6	T	C	11: 96,047,492 (GRCm39)	L697P	possibly damaging	Het
Ttn	T	G	2: 76,549,239 (GRCm39)	T31813P	probably damaging	Het
Ttn	A	T	2: 76,571,932 (GRCm39)	D26320E	probably damaging	Het
Uba6	A	G	5: 86,307,061 (GRCm39)		probably null	Het
Usp1	C	G	4: 98,819,185 (GRCm39)	Q216E	probably benign	Het
Wdr64	C	A	1: 175,599,850 (GRCm39)	P559H	probably benign	Het
Zfat	T	C	15: 68,056,315 (GRCm39)	Y247C	probably damaging	Het
Zfp979	A	T	4: 147,698,219 (GRCm39)	N163K	probably damaging	Het

Other mutations in Vcan

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00434:Vcan	APN	13	89,852,821 (GRCm39)	missense	probably damaging	1.00
IGL00502:Vcan	APN	13	89,840,438 (GRCm39)	missense	probably benign
IGL00504:Vcan	APN	13	89,839,394 (GRCm39)	missense	possibly damaging	0.70
IGL00566:Vcan	APN	13	89,837,098 (GRCm39)	missense	probably benign	0.01
IGL00701:Vcan	APN	13	89,851,845 (GRCm39)	missense	probably benign
IGL00743:Vcan	APN	13	89,873,425 (GRCm39)	missense	probably damaging	0.98
IGL00962:Vcan	APN	13	89,810,171 (GRCm39)	missense	probably damaging	1.00
IGL01085:Vcan	APN	13	89,828,077 (GRCm39)	missense	probably damaging	1.00
IGL01317:Vcan	APN	13	89,839,787 (GRCm39)	missense	probably benign	0.00
IGL01349:Vcan	APN	13	89,852,062 (GRCm39)	missense	probably damaging	0.98
IGL01391:Vcan	APN	13	89,852,288 (GRCm39)	missense	probably benign	0.19
IGL01644:Vcan	APN	13	89,836,794 (GRCm39)	missense	probably benign	0.13
IGL01657:Vcan	APN	13	89,838,705 (GRCm39)	missense	probably damaging	1.00
IGL01707:Vcan	APN	13	89,837,864 (GRCm39)	missense	probably damaging	1.00
IGL01764:Vcan	APN	13	89,873,507 (GRCm39)	missense	probably damaging	1.00
IGL01920:Vcan	APN	13	89,837,324 (GRCm39)	missense	probably benign	0.04
IGL01989:Vcan	APN	13	89,837,478 (GRCm39)	missense	possibly damaging	0.86
IGL01999:Vcan	APN	13	89,832,557 (GRCm39)	missense	probably damaging	1.00
IGL02083:Vcan	APN	13	89,873,684 (GRCm39)	missense	probably damaging	1.00
IGL02160:Vcan	APN	13	89,832,612 (GRCm39)	missense	probably damaging	1.00
IGL02217:Vcan	APN	13	89,851,196 (GRCm39)	missense	probably damaging	1.00
IGL02522:Vcan	APN	13	89,852,968 (GRCm39)	missense	probably benign	0.00
IGL02527:Vcan	APN	13	89,838,776 (GRCm39)	missense	possibly damaging	0.95
IGL02926:Vcan	APN	13	89,836,742 (GRCm39)	missense	probably damaging	0.98
IGL03061:Vcan	APN	13	89,851,394 (GRCm39)	missense	probably benign	0.25
IGL03331:Vcan	APN	13	89,810,051 (GRCm39)	missense	probably damaging	1.00
IGL03352:Vcan	APN	13	89,853,125 (GRCm39)	missense	probably benign	0.00
R0041:Vcan	UTSW	13	89,810,104 (GRCm39)	missense	probably damaging	1.00
R0102:Vcan	UTSW	13	89,851,787 (GRCm39)	missense	probably benign	0.01
R0102:Vcan	UTSW	13	89,851,787 (GRCm39)	missense	probably benign	0.01
R0109:Vcan	UTSW	13	89,826,192 (GRCm39)	critical splice donor site	probably null
R0139:Vcan	UTSW	13	89,839,380 (GRCm39)	missense	probably damaging	1.00
R0295:Vcan	UTSW	13	89,860,310 (GRCm39)	missense	probably benign	0.06
R0375:Vcan	UTSW	13	89,839,394 (GRCm39)	missense	probably damaging	0.99
R0379:Vcan	UTSW	13	89,851,665 (GRCm39)	missense	probably damaging	0.99
R0457:Vcan	UTSW	13	89,851,318 (GRCm39)	missense	possibly damaging	0.78
R0482:Vcan	UTSW	13	89,826,264 (GRCm39)	missense	probably damaging	1.00
R0485:Vcan	UTSW	13	89,852,779 (GRCm39)	missense	possibly damaging	0.92
R0532:Vcan	UTSW	13	89,851,891 (GRCm39)	missense	probably damaging	0.99
R0561:Vcan	UTSW	13	89,879,583 (GRCm39)	missense	possibly damaging	0.86
R0561:Vcan	UTSW	13	89,860,372 (GRCm39)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,860,386 (GRCm39)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,852,825 (GRCm39)	missense	probably damaging	0.99
R0680:Vcan	UTSW	13	89,827,941 (GRCm39)	missense	probably damaging	1.00
R0849:Vcan	UTSW	13	89,853,072 (GRCm39)	missense	possibly damaging	0.75
R1006:Vcan	UTSW	13	89,833,196 (GRCm39)	critical splice donor site	probably null
R1104:Vcan	UTSW	13	89,840,529 (GRCm39)	missense	probably damaging	1.00
R1118:Vcan	UTSW	13	89,853,782 (GRCm39)	missense	probably damaging	1.00
R1137:Vcan	UTSW	13	89,852,422 (GRCm39)	missense	probably damaging	1.00
R1199:Vcan	UTSW	13	89,827,913 (GRCm39)	splice site	probably null
R1219:Vcan	UTSW	13	89,828,023 (GRCm39)	missense	probably damaging	1.00
R1296:Vcan	UTSW	13	89,805,675 (GRCm39)	missense	probably damaging	1.00
R1332:Vcan	UTSW	13	89,841,174 (GRCm39)	missense	probably damaging	1.00
R1336:Vcan	UTSW	13	89,841,174 (GRCm39)	missense	probably damaging	1.00
R1403:Vcan	UTSW	13	89,836,603 (GRCm39)	missense	probably benign	0.00
R1403:Vcan	UTSW	13	89,836,603 (GRCm39)	missense	probably benign	0.00
R1546:Vcan	UTSW	13	89,841,075 (GRCm39)	missense	probably damaging	0.99
R1604:Vcan	UTSW	13	89,837,780 (GRCm39)	missense	probably benign	0.42
R1616:Vcan	UTSW	13	89,853,782 (GRCm39)	missense	probably damaging	1.00
R1636:Vcan	UTSW	13	89,851,786 (GRCm39)	missense	possibly damaging	0.90
R1654:Vcan	UTSW	13	89,810,065 (GRCm39)	missense	probably damaging	1.00
R1680:Vcan	UTSW	13	89,851,666 (GRCm39)	missense	probably benign	0.19
R1694:Vcan	UTSW	13	89,836,602 (GRCm39)	missense	probably damaging	0.98
R1712:Vcan	UTSW	13	89,869,894 (GRCm39)	missense	probably damaging	1.00
R1754:Vcan	UTSW	13	89,852,854 (GRCm39)	missense	probably benign	0.01
R1756:Vcan	UTSW	13	89,839,800 (GRCm39)	missense	probably benign	0.05
R1824:Vcan	UTSW	13	89,853,331 (GRCm39)	missense	possibly damaging	0.75
R1852:Vcan	UTSW	13	89,853,511 (GRCm39)	missense	probably damaging	0.99
R1868:Vcan	UTSW	13	89,838,990 (GRCm39)	missense	probably benign	0.12
R1920:Vcan	UTSW	13	89,841,134 (GRCm39)	missense	probably damaging	1.00
R1932:Vcan	UTSW	13	89,853,653 (GRCm39)	missense	possibly damaging	0.78
R1934:Vcan	UTSW	13	89,851,045 (GRCm39)	missense	probably damaging	1.00
R1942:Vcan	UTSW	13	89,851,543 (GRCm39)	missense	probably benign	0.01
R1964:Vcan	UTSW	13	89,840,861 (GRCm39)	missense	probably benign	0.02
R1970:Vcan	UTSW	13	89,837,157 (GRCm39)	missense	probably damaging	1.00
R2045:Vcan	UTSW	13	89,839,104 (GRCm39)	missense	probably benign	0.00
R2110:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2111:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2112:Vcan	UTSW	13	89,841,422 (GRCm39)	missense	probably damaging	1.00
R2136:Vcan	UTSW	13	89,837,856 (GRCm39)	missense	probably damaging	1.00
R2158:Vcan	UTSW	13	89,851,648 (GRCm39)	missense	possibly damaging	0.68
R2376:Vcan	UTSW	13	89,851,529 (GRCm39)	missense	possibly damaging	0.80
R2385:Vcan	UTSW	13	89,837,568 (GRCm39)	missense	probably damaging	1.00
R2443:Vcan	UTSW	13	89,852,794 (GRCm39)	missense	probably damaging	1.00
R2876:Vcan	UTSW	13	89,852,356 (GRCm39)	missense	probably damaging	1.00
R3607:Vcan	UTSW	13	89,851,420 (GRCm39)	missense	probably damaging	0.98
R4042:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4043:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4044:Vcan	UTSW	13	89,840,662 (GRCm39)	missense	probably benign	0.35
R4065:Vcan	UTSW	13	89,828,006 (GRCm39)	missense	probably damaging	1.00
R4161:Vcan	UTSW	13	89,833,277 (GRCm39)	missense	probably damaging	1.00
R4178:Vcan	UTSW	13	89,873,666 (GRCm39)	missense	probably damaging	1.00
R4290:Vcan	UTSW	13	89,873,605 (GRCm39)	missense	probably damaging	1.00
R4530:Vcan	UTSW	13	89,852,147 (GRCm39)	missense	probably damaging	0.97
R4666:Vcan	UTSW	13	89,828,053 (GRCm39)	missense	probably damaging	1.00
R4785:Vcan	UTSW	13	89,853,908 (GRCm39)	missense	probably damaging	1.00
R4870:Vcan	UTSW	13	89,852,858 (GRCm39)	missense	probably benign	0.01
R4973:Vcan	UTSW	13	89,836,961 (GRCm39)	missense	probably benign	0.30
R5037:Vcan	UTSW	13	89,852,096 (GRCm39)	missense	probably damaging	1.00
R5104:Vcan	UTSW	13	89,805,591 (GRCm39)	intron	probably benign
R5124:Vcan	UTSW	13	89,873,636 (GRCm39)	missense	probably damaging	1.00
R5129:Vcan	UTSW	13	89,838,359 (GRCm39)	missense	probably damaging	1.00
R5198:Vcan	UTSW	13	89,838,991 (GRCm39)	missense	probably damaging	1.00
R5240:Vcan	UTSW	13	89,840,651 (GRCm39)	missense	probably benign	0.08
R5254:Vcan	UTSW	13	89,839,719 (GRCm39)	missense	probably damaging	0.99
R5280:Vcan	UTSW	13	89,838,405 (GRCm39)	missense	probably benign	0.00
R5522:Vcan	UTSW	13	89,839,929 (GRCm39)	missense	possibly damaging	0.62
R5557:Vcan	UTSW	13	89,851,231 (GRCm39)	missense	possibly damaging	0.77
R5568:Vcan	UTSW	13	89,836,790 (GRCm39)	missense	probably damaging	1.00
R5578:Vcan	UTSW	13	89,839,622 (GRCm39)	missense	probably benign	0.01
R5627:Vcan	UTSW	13	89,839,254 (GRCm39)	frame shift	probably null
R5687:Vcan	UTSW	13	89,826,253 (GRCm39)	missense	probably damaging	1.00
R5752:Vcan	UTSW	13	89,828,069 (GRCm39)	missense	probably damaging	1.00
R5879:Vcan	UTSW	13	89,852,071 (GRCm39)	missense	probably damaging	0.99
R5941:Vcan	UTSW	13	89,840,810 (GRCm39)	missense	probably damaging	0.98
R6113:Vcan	UTSW	13	89,805,655 (GRCm39)	nonsense	probably null
R6135:Vcan	UTSW	13	89,838,045 (GRCm39)	missense	probably benign	0.36
R6252:Vcan	UTSW	13	89,839,339 (GRCm39)	nonsense	probably null
R6280:Vcan	UTSW	13	89,873,492 (GRCm39)	missense	probably damaging	1.00
R6317:Vcan	UTSW	13	89,839,716 (GRCm39)	missense	probably benign	0.22
R6327:Vcan	UTSW	13	89,852,951 (GRCm39)	missense	probably damaging	0.99
R6460:Vcan	UTSW	13	89,838,806 (GRCm39)	missense	possibly damaging	0.61
R6669:Vcan	UTSW	13	89,852,850 (GRCm39)	missense	probably benign	0.21
R6744:Vcan	UTSW	13	89,853,301 (GRCm39)	missense	probably damaging	1.00
R6819:Vcan	UTSW	13	89,853,244 (GRCm39)	missense	probably benign	0.00
R6880:Vcan	UTSW	13	89,860,500 (GRCm39)	missense	probably damaging	1.00
R6956:Vcan	UTSW	13	89,837,550 (GRCm39)	missense	probably damaging	0.99
R6971:Vcan	UTSW	13	89,826,252 (GRCm39)	missense	probably damaging	1.00
R6985:Vcan	UTSW	13	89,828,075 (GRCm39)	missense	probably damaging	1.00
R6994:Vcan	UTSW	13	89,841,526 (GRCm39)	missense	possibly damaging	0.94
R6997:Vcan	UTSW	13	89,838,737 (GRCm39)	missense	probably damaging	0.98
R7029:Vcan	UTSW	13	89,838,360 (GRCm39)	missense	probably damaging	1.00
R7066:Vcan	UTSW	13	89,853,805 (GRCm39)	missense	probably damaging	1.00
R7156:Vcan	UTSW	13	89,837,229 (GRCm39)	missense	possibly damaging	0.95
R7171:Vcan	UTSW	13	89,873,710 (GRCm39)	missense	probably damaging	1.00
R7176:Vcan	UTSW	13	89,837,055 (GRCm39)	missense	probably benign	0.01
R7229:Vcan	UTSW	13	89,853,389 (GRCm39)	missense	possibly damaging	0.87
R7250:Vcan	UTSW	13	89,879,576 (GRCm39)	critical splice donor site	probably null
R7250:Vcan	UTSW	13	89,869,805 (GRCm39)	missense	probably damaging	1.00
R7262:Vcan	UTSW	13	89,853,280 (GRCm39)	missense	possibly damaging	0.62
R7289:Vcan	UTSW	13	89,840,852 (GRCm39)	nonsense	probably null
R7299:Vcan	UTSW	13	89,853,385 (GRCm39)	missense	probably benign
R7301:Vcan	UTSW	13	89,853,385 (GRCm39)	missense	probably benign
R7425:Vcan	UTSW	13	89,837,951 (GRCm39)	missense	probably damaging	0.99
R7514:Vcan	UTSW	13	89,852,237 (GRCm39)	missense	probably damaging	0.97
R7579:Vcan	UTSW	13	89,840,577 (GRCm39)	missense	probably damaging	1.00
R7618:Vcan	UTSW	13	89,840,342 (GRCm39)	missense	probably damaging	0.99
R7655:Vcan	UTSW	13	89,833,233 (GRCm39)	missense	probably damaging	1.00
R7656:Vcan	UTSW	13	89,833,233 (GRCm39)	missense	probably damaging	1.00
R7676:Vcan	UTSW	13	89,839,908 (GRCm39)	missense	probably damaging	1.00
R7719:Vcan	UTSW	13	89,852,738 (GRCm39)	missense	probably damaging	0.98
R7753:Vcan	UTSW	13	89,837,442 (GRCm39)	missense	probably damaging	1.00
R7762:Vcan	UTSW	13	89,841,056 (GRCm39)	missense	probably damaging	1.00
R7778:Vcan	UTSW	13	89,836,773 (GRCm39)	missense	probably damaging	1.00
R7824:Vcan	UTSW	13	89,836,773 (GRCm39)	missense	probably damaging	1.00
R7995:Vcan	UTSW	13	89,839,977 (GRCm39)	missense	probably benign
R7998:Vcan	UTSW	13	89,852,446 (GRCm39)	missense	probably damaging	1.00
R8033:Vcan	UTSW	13	89,852,479 (GRCm39)	missense	probably benign	0.04
R8061:Vcan	UTSW	13	89,805,409 (GRCm39)	missense	probably benign	0.45
R8103:Vcan	UTSW	13	89,851,439 (GRCm39)	nonsense	probably null
R8103:Vcan	UTSW	13	89,805,777 (GRCm39)	missense	probably damaging	1.00
R8124:Vcan	UTSW	13	89,852,373 (GRCm39)	missense	possibly damaging	0.93
R8162:Vcan	UTSW	13	89,853,106 (GRCm39)	nonsense	probably null
R8166:Vcan	UTSW	13	89,840,855 (GRCm39)	missense	probably benign	0.02
R8274:Vcan	UTSW	13	89,853,089 (GRCm39)	missense	probably benign	0.02
R8284:Vcan	UTSW	13	89,852,454 (GRCm39)	missense	possibly damaging	0.68
R8417:Vcan	UTSW	13	89,836,862 (GRCm39)	missense	probably benign	0.19
R8696:Vcan	UTSW	13	89,839,217 (GRCm39)	missense	probably benign	0.00
R8738:Vcan	UTSW	13	89,840,439 (GRCm39)	missense	probably benign	0.17
R8792:Vcan	UTSW	13	89,840,230 (GRCm39)	missense	possibly damaging	0.91
R9049:Vcan	UTSW	13	89,826,224 (GRCm39)	missense	probably damaging	1.00
R9074:Vcan	UTSW	13	89,839,146 (GRCm39)	missense	possibly damaging	0.95
R9095:Vcan	UTSW	13	89,852,644 (GRCm39)	missense	probably benign	0.32
R9172:Vcan	UTSW	13	89,828,050 (GRCm39)	missense	probably damaging	1.00
R9199:Vcan	UTSW	13	89,838,615 (GRCm39)	nonsense	probably null
R9259:Vcan	UTSW	13	89,838,989 (GRCm39)	missense	probably damaging	0.99
R9455:Vcan	UTSW	13	89,837,452 (GRCm39)	missense	probably damaging	1.00
R9476:Vcan	UTSW	13	89,851,531 (GRCm39)	missense	possibly damaging	0.95
R9477:Vcan	UTSW	13	89,841,128 (GRCm39)	missense	probably damaging	1.00
R9555:Vcan	UTSW	13	89,839,659 (GRCm39)	missense
R9579:Vcan	UTSW	13	89,837,713 (GRCm39)	missense	possibly damaging	0.67
R9606:Vcan	UTSW	13	89,853,491 (GRCm39)	missense	probably damaging	1.00
R9645:Vcan	UTSW	13	89,841,081 (GRCm39)	missense	probably benign	0.00
R9659:Vcan	UTSW	13	89,839,860 (GRCm39)	missense	probably damaging	0.99
R9766:Vcan	UTSW	13	89,839,247 (GRCm39)	missense	probably benign	0.00
R9778:Vcan	UTSW	13	89,837,930 (GRCm39)	missense	probably damaging	1.00
X0058:Vcan	UTSW	13	89,840,612 (GRCm39)	missense	probably benign	0.21
X0065:Vcan	UTSW	13	89,853,868 (GRCm39)	missense	probably damaging	0.96
Z1176:Vcan	UTSW	13	89,840,690 (GRCm39)	missense	probably benign	0.10
Z1177:Vcan	UTSW	13	89,852,192 (GRCm39)	missense	probably damaging	1.00
Z1177:Vcan	UTSW	13	89,851,907 (GRCm39)	nonsense	probably null
Z1177:Vcan	UTSW	13	89,851,643 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TGGTATTTCATCAACTGTGTACGG -3'
(R):5'- ATCTGATCAGAGCACTCGGGTC -3'

Sequencing Primer
(F):5'- ACTGTGGATATTCCCTCTAC -3'
(R):5'- GGTCTTTAGCCAAATCCCCGAAG -3'

Posted On

2021-08-02