Mutagenetix > Incidental Mutation

Incidental Mutation 'R8891:Zfp64'

677671

Institutional Source

Beutler Lab

Gene Symbol

Zfp64

Ensembl Gene

ENSMUSG00000027551

Gene Name

zinc finger protein 64

Synonyms

MMRRC Submission

068753-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.863) question?

Stock #

R8891 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

168735251-168797507 bp(-) (GRCm39)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

A to T at 168797083 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 1 (M1K)

Ref Sequence

ENSEMBL: ENSMUSP00000085285 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087971] [ENSMUST00000109161] [ENSMUST00000109162]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000087971
AA Change: M1K

PolyPhen 2 Score 0.366 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000085285
Gene: ENSMUSG00000027551
AA Change: M1K

Domain	Start	End	E-Value	Type
ZnF_C2H2	31	51	5.54e1	SMART
low complexity region	71	93	N/A	INTRINSIC
ZnF_C2H2	142	167	1.68e1	SMART
ZnF_C2H2	173	195	2.24e-3	SMART
ZnF_C2H2	201	223	1.58e-3	SMART
ZnF_C2H2	229	251	2.24e-3	SMART
ZnF_C2H2	257	279	3.89e-3	SMART
ZnF_C2H2	285	308	6.32e-3	SMART
ZnF_C2H2	313	335	2.05e-2	SMART
ZnF_C2H2	341	363	1.95e-3	SMART
ZnF_C2H2	369	392	1.36e-2	SMART
ZnF_C2H2	423	445	1.47e-3	SMART
low complexity region	503	522	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000109161
AA Change: M1K

PolyPhen 2 Score 0.937 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000104789
Gene: ENSMUSG00000027551
AA Change: M1K

Domain	Start	End	E-Value	Type
ZnF_C2H2	31	51	5.54e1	SMART
low complexity region	71	93	N/A	INTRINSIC
ZnF_C2H2	142	167	1.68e1	SMART
ZnF_C2H2	173	195	2.24e-3	SMART
ZnF_C2H2	201	223	1.58e-3	SMART
ZnF_C2H2	229	251	2.24e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000109162
AA Change: M1K

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000104790
Gene: ENSMUSG00000027551
AA Change: M1K

Domain	Start	End	E-Value	Type
ZnF_C2H2	31	51	5.54e1	SMART
low complexity region	71	93	N/A	INTRINSIC
ZnF_C2H2	142	167	1.68e1	SMART
ZnF_C2H2	173	195	2.24e-3	SMART
ZnF_C2H2	201	223	1.58e-3	SMART
ZnF_C2H2	229	251	2.24e-3	SMART
ZnF_C2H2	297	322	5.2e0	SMART
ZnF_C2H2	328	350	4.17e-3	SMART
ZnF_C2H2	356	378	6.78e-3	SMART
ZnF_C2H2	384	406	2.24e-3	SMART
ZnF_C2H2	412	434	1.79e-2	SMART
ZnF_C2H2	440	463	9.58e-3	SMART
ZnF_C2H2	465	487	3.78e-1	SMART
ZnF_C2H2	493	515	3.89e-3	SMART
ZnF_C2H2	521	544	1.92e-2	SMART
ZnF_C2H2	578	600	5.34e-1	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	T	3: 137,772,520 (GRCm39)	K570*	probably null	Het
Abi1	T	C	2: 22,861,262 (GRCm39)	I99V	probably damaging	Het
Abtb3	G	A	10: 85,223,958 (GRCm39)	G256R	unknown	Het
Adgrg7	T	A	16: 56,572,762 (GRCm39)	E351D	probably benign	Het
Ankrd34c	T	G	9: 89,612,143 (GRCm39)	K66T	probably damaging	Het
Asap2	C	T	12: 21,162,144 (GRCm39)	R34W	probably damaging	Het
Atr	A	G	9: 95,787,813 (GRCm39)	T1469A	probably benign	Het
Ccdc168	T	C	1: 44,096,284 (GRCm39)	I1605V	probably benign	Het
Celsr1	G	T	15: 85,822,194 (GRCm39)	R1708S	probably benign	Het
Chmp2a	T	A	7: 12,767,840 (GRCm39)	E28D	probably benign	Het
Cldn24	A	T	8: 48,275,281 (GRCm39)	N35I	probably benign	Het
Coa6	G	C	8: 127,149,570 (GRCm39)		probably null	Het
Copa	C	T	1: 171,946,818 (GRCm39)	R1009C	probably damaging	Het
Crybb2	G	A	5: 113,209,913 (GRCm39)	T150M	possibly damaging	Het
Ctnnd2	A	T	15: 30,620,076 (GRCm39)	T143S	probably benign	Het
Eid3	A	G	10: 82,702,992 (GRCm39)	N151S	probably damaging	Het
Fbxo17	G	T	7: 28,434,733 (GRCm39)	V173L	possibly damaging	Het
Fmn2	CCCTCCTCTCCCTGGAATGGGAATACCTCCCCCACCTCCTCTCCCTGGAATGGGAATACCTCCCCCACCTCCTCTCCCTGGAATGGGAATATCTCCCCTACCTCCTCTCCCTGGAATGGGAATACCTCC	CCCTCCTCTCCCTGGAATGGGAATACCTCCCCCACCTCCTCTCCCTGGAATGGGAATATCTCCCCTACCTCCTCTCCCTGGAATGGGAATACCTCC	1: 174,436,769 (GRCm39)		probably benign	Het
Galntl6	T	C	8: 58,415,433 (GRCm39)	N240S	probably damaging	Het
Gck	G	T	11: 5,851,733 (GRCm39)	S445R	probably damaging	Het
Gm5114	C	A	7: 39,057,718 (GRCm39)	V634F	probably benign	Het
Gnat2	G	A	3: 108,005,634 (GRCm39)	D200N		Het
H13	A	G	2: 152,546,049 (GRCm39)	N390S	probably benign	Het
Helz	T	A	11: 107,552,842 (GRCm39)	M1206K	probably damaging	Het
Itpr3	G	A	17: 27,337,651 (GRCm39)		probably benign	Het
Kank1	G	A	19: 25,387,439 (GRCm39)	G371R	probably benign	Het
Kif13b	A	G	14: 64,982,326 (GRCm39)	T513A	probably damaging	Het
L3mbtl4	T	C	17: 68,762,781 (GRCm39)	S105P	possibly damaging	Het
Lpo	T	A	11: 87,697,848 (GRCm39)	E653V	probably benign	Het
Lyst	A	G	13: 13,887,435 (GRCm39)	D3088G	possibly damaging	Het
Man2b1	C	A	8: 85,811,084 (GRCm39)	H72N	probably damaging	Het
Mast3	A	T	8: 71,233,801 (GRCm39)	H981Q	probably damaging	Het
Meioc	T	A	11: 102,559,246 (GRCm39)	I56N	probably benign	Het
Mul1	A	C	4: 138,162,164 (GRCm39)	K32Q	probably benign	Het
Oas1c	C	T	5: 120,946,126 (GRCm39)	S124N	probably benign	Het
Omg	T	A	11: 79,393,829 (GRCm39)	K10*	probably null	Het
Or2a52	A	G	6: 43,144,750 (GRCm39)	I253V	probably benign	Het
Or4k52	A	T	2: 111,611,186 (GRCm39)	I174F	probably damaging	Het
Or6c88	A	G	10: 129,407,046 (GRCm39)	H174R	probably damaging	Het
Pcdhb14	T	A	18: 37,582,692 (GRCm39)	N599K	probably damaging	Het
Pcdhb18	C	A	18: 37,623,700 (GRCm39)	N343K	probably damaging	Het
Phf7	C	A	14: 30,971,613 (GRCm39)		probably benign	Het
Pramel15	C	T	4: 144,099,397 (GRCm39)	C456Y	probably damaging	Het
Qdpr	T	C	5: 45,604,982 (GRCm39)	N42S	probably damaging	Het
Rbm44	T	A	1: 91,090,136 (GRCm39)	D716E	probably benign	Het
Rprd2	A	C	3: 95,671,367 (GRCm39)	H1345Q	possibly damaging	Het
Rsf1	G	GACGGCGGCT	7: 97,229,116 (GRCm39)		probably benign	Het
Ryr2	G	A	13: 11,814,768 (GRCm39)	A668V	probably damaging	Het
Sag	T	A	1: 87,759,683 (GRCm39)	L307Q	probably damaging	Het
Sash1	G	A	10: 8,603,734 (GRCm39)	P1106L	probably damaging	Het
Sec16b	T	A	1: 157,382,409 (GRCm39)	I615N	probably damaging	Het
Sfi1	A	ATCTTCCCAAAGCCAGTGC	11: 3,103,384 (GRCm39)		probably benign	Het
Slco1b2	T	C	6: 141,628,993 (GRCm39)	V600A	probably benign	Het
Sucnr1	A	G	3: 59,994,263 (GRCm39)	T264A	probably benign	Het
Tbx3	A	G	5: 119,809,983 (GRCm39)		probably benign	Het
Tigd5	T	C	15: 75,783,069 (GRCm39)	V477A	possibly damaging	Het
Tle7	T	A	8: 110,836,763 (GRCm39)	S216R	possibly damaging	Het
Ttc17	G	A	2: 94,192,764 (GRCm39)	A89V	probably damaging	Het
Twsg1	A	G	17: 66,255,657 (GRCm39)	I39T		Het
Zfp184	T	C	13: 22,143,512 (GRCm39)	F406S	probably damaging	Het
Zfp429	A	T	13: 67,538,830 (GRCm39)	C205S	probably damaging	Het
Zfp59	T	A	7: 27,554,313 (GRCm39)	D588E	probably benign	Het

Other mutations in Zfp64

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00155:Zfp64	APN	2	168,768,601 (GRCm39)	missense	probably benign	0.06
IGL01479:Zfp64	APN	2	168,793,599 (GRCm39)	missense	probably damaging	1.00
IGL02320:Zfp64	APN	2	168,768,118 (GRCm39)	missense	probably damaging	0.98
IGL03411:Zfp64	APN	2	168,793,462 (GRCm39)	splice site	probably null
PIT4362001:Zfp64	UTSW	2	168,767,735 (GRCm39)	missense	probably benign	0.23
R0033:Zfp64	UTSW	2	168,767,635 (GRCm39)	missense	possibly damaging	0.94
R0033:Zfp64	UTSW	2	168,767,635 (GRCm39)	missense	possibly damaging	0.94
R0230:Zfp64	UTSW	2	168,754,150 (GRCm39)	splice site	probably benign
R0325:Zfp64	UTSW	2	168,767,960 (GRCm39)	missense	probably benign	0.00
R0689:Zfp64	UTSW	2	168,777,121 (GRCm39)	splice site	probably benign
R1741:Zfp64	UTSW	2	168,768,238 (GRCm39)	missense	probably benign	0.05
R2054:Zfp64	UTSW	2	168,767,728 (GRCm39)	missense	probably damaging	1.00
R2133:Zfp64	UTSW	2	168,782,663 (GRCm39)	missense	probably damaging	1.00
R2254:Zfp64	UTSW	2	168,768,662 (GRCm39)	missense	probably damaging	0.96
R4093:Zfp64	UTSW	2	168,767,855 (GRCm39)	missense	probably benign	0.00
R4094:Zfp64	UTSW	2	168,767,855 (GRCm39)	missense	probably benign	0.00
R4257:Zfp64	UTSW	2	168,768,298 (GRCm39)	missense	probably damaging	1.00
R4630:Zfp64	UTSW	2	168,768,463 (GRCm39)	missense	possibly damaging	0.94
R4642:Zfp64	UTSW	2	168,776,851 (GRCm39)	missense	probably benign	0.22
R4817:Zfp64	UTSW	2	168,768,032 (GRCm39)	missense	probably benign
R4880:Zfp64	UTSW	2	168,736,297 (GRCm39)	missense	probably damaging	1.00
R5051:Zfp64	UTSW	2	168,768,304 (GRCm39)	missense	probably damaging	0.98
R5155:Zfp64	UTSW	2	168,748,885 (GRCm39)	missense	probably benign	0.17
R5562:Zfp64	UTSW	2	168,767,642 (GRCm39)	missense	probably benign	0.00
R5619:Zfp64	UTSW	2	168,741,735 (GRCm39)	missense	probably damaging	0.97
R5619:Zfp64	UTSW	2	168,741,734 (GRCm39)	missense	probably damaging	0.99
R5620:Zfp64	UTSW	2	168,741,888 (GRCm39)	missense	possibly damaging	0.70
R5919:Zfp64	UTSW	2	168,768,419 (GRCm39)	missense	probably benign	0.05
R6156:Zfp64	UTSW	2	168,768,088 (GRCm39)	missense	probably benign	0.34
R6364:Zfp64	UTSW	2	168,754,186 (GRCm39)	missense	probably damaging	1.00
R6488:Zfp64	UTSW	2	168,777,129 (GRCm39)	critical splice donor site	probably null
R6512:Zfp64	UTSW	2	168,735,997 (GRCm39)	missense	probably benign	0.00
R6588:Zfp64	UTSW	2	168,768,827 (GRCm39)	missense	probably damaging	1.00
R7103:Zfp64	UTSW	2	168,768,357 (GRCm39)	missense	probably benign	0.00
R7313:Zfp64	UTSW	2	168,741,810 (GRCm39)	missense	probably damaging	1.00
R7470:Zfp64	UTSW	2	168,767,731 (GRCm39)	missense	probably damaging	1.00
R7529:Zfp64	UTSW	2	168,735,992 (GRCm39)	missense	probably benign
R7560:Zfp64	UTSW	2	168,767,585 (GRCm39)	missense	probably damaging	1.00
R7963:Zfp64	UTSW	2	168,793,538 (GRCm39)	missense	probably benign	0.32
R8037:Zfp64	UTSW	2	168,741,932 (GRCm39)	missense	probably damaging	1.00
R8038:Zfp64	UTSW	2	168,741,932 (GRCm39)	missense	probably damaging	1.00
R8825:Zfp64	UTSW	2	168,793,552 (GRCm39)	missense	probably benign
R8840:Zfp64	UTSW	2	168,768,635 (GRCm39)	missense	probably benign
R9062:Zfp64	UTSW	2	168,767,747 (GRCm39)	missense	probably benign
R9592:Zfp64	UTSW	2	168,768,118 (GRCm39)	missense	probably damaging	1.00
R9681:Zfp64	UTSW	2	168,793,680 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTACTGCAAGAGTTCCAGGC -3'
(R):5'- GTACTCGGGTCTTAGACTCCAC -3'

Sequencing Primer
(F):5'- AAGAGTTCCAGGCGGCTC -3'
(R):5'- GGGTCTTAGACTCCACGACCTTTC -3'

Posted On

2021-08-02