Mutagenetix > Incidental Mutation

Incidental Mutation 'R8891:Meioc'

677705

Institutional Source

Beutler Lab

Gene Symbol

Meioc

Ensembl Gene

ENSMUSG00000051455

Gene Name

meiosis specific with coiled-coil domain

Synonyms

Gm1564, LOC380729, LOC268491

MMRRC Submission

068753-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.133) question?

Stock #

R8891 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

102663716-102682237 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 102668420 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 56 (I56N)

Ref Sequence

ENSEMBL: ENSMUSP00000116246 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100378] [ENSMUST00000156590]

AlphaFold

A2AG06

Predicted Effect

probably benign
Transcript: ENSMUST00000100378
AA Change: I112N

PolyPhen 2 Score 0.428 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000097947
Gene: ENSMUSG00000051455
AA Change: I112N

Domain	Start	End	E-Value	Type
low complexity region	526	537	N/A	INTRINSIC
low complexity region	712	728	N/A	INTRINSIC
Pfam:DUF4582	757	922	5.1e-89	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000156590
AA Change: I56N

PolyPhen 2 Score 0.428 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000116246
Gene: ENSMUSG00000051455
AA Change: I56N

Domain	Start	End	E-Value	Type
low complexity region	470	481	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit infertility with small gonads, absent germ cells and arrested meiosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	T	3: 138,066,759 (GRCm38)	K570*	probably null	Het
Abi1	T	C	2: 22,971,250 (GRCm38)	I99V	probably damaging	Het
Abtb3	G	A	10: 85,388,094 (GRCm38)	G256R	unknown	Het
Adgrg7	T	A	16: 56,752,399 (GRCm38)	E351D	probably benign	Het
Ankrd34c	T	G	9: 89,730,090 (GRCm38)	K66T	probably damaging	Het
Asap2	C	T	12: 21,112,143 (GRCm38)	R34W	probably damaging	Het
Atr	A	G	9: 95,905,760 (GRCm38)	T1469A	probably benign	Het
Ccdc168	T	C	1: 44,057,124 (GRCm38)	I1605V	probably benign	Het
Celsr1	G	T	15: 85,937,993 (GRCm38)	R1708S	probably benign	Het
Chmp2a	T	A	7: 13,033,913 (GRCm38)	E28D	probably benign	Het
Cldn24	A	T	8: 47,822,246 (GRCm38)	N35I	probably benign	Het
Coa6	G	C	8: 126,422,831 (GRCm38)		probably null	Het
Copa	C	T	1: 172,119,251 (GRCm38)	R1009C	probably damaging	Het
Crybb2	G	A	5: 113,062,047 (GRCm38)	T150M	possibly damaging	Het
Ctnnd2	A	T	15: 30,619,930 (GRCm38)	T143S	probably benign	Het
Eid3	A	G	10: 82,867,158 (GRCm38)	N151S	probably damaging	Het
Fbxo17	G	T	7: 28,735,308 (GRCm38)	V173L	possibly damaging	Het
Fmn2	CCCTCCTCTCCCTGGAATGGGAATACCTCCCCCACCTCCTCTCCCTGGAATGGGAATACCTCCCCCACCTCCTCTCCCTGGAATGGGAATATCTCCCCTACCTCCTCTCCCTGGAATGGGAATACCTCC	CCCTCCTCTCCCTGGAATGGGAATACCTCCCCCACCTCCTCTCCCTGGAATGGGAATATCTCCCCTACCTCCTCTCCCTGGAATGGGAATACCTCC	1: 174,609,203 (GRCm38)		probably benign	Het
Galntl6	T	C	8: 57,962,399 (GRCm38)	N240S	probably damaging	Het
Gck	G	T	11: 5,901,733 (GRCm38)	S445R	probably damaging	Het
Gm5114	C	A	7: 39,408,294 (GRCm38)	V634F	probably benign	Het
Gnat2	G	A	3: 108,098,318 (GRCm38)	D200N		Het
H13	A	G	2: 152,704,129 (GRCm38)	N390S	probably benign	Het
Helz	T	A	11: 107,662,016 (GRCm38)	M1206K	probably damaging	Het
Itpr3	G	A	17: 27,118,677 (GRCm38)		probably benign	Het
Kank1	G	A	19: 25,410,075 (GRCm38)	G371R	probably benign	Het
Kif13b	A	G	14: 64,744,877 (GRCm38)	T513A	probably damaging	Het
L3mbtl4	T	C	17: 68,455,786 (GRCm38)	S105P	possibly damaging	Het
Lpo	T	A	11: 87,807,022 (GRCm38)	E653V	probably benign	Het
Lyst	A	G	13: 13,712,850 (GRCm38)	D3088G	possibly damaging	Het
Man2b1	C	A	8: 85,084,455 (GRCm38)	H72N	probably damaging	Het
Mast3	A	T	8: 70,781,157 (GRCm38)	H981Q	probably damaging	Het
Mul1	A	C	4: 138,434,853 (GRCm38)	K32Q	probably benign	Het
Oas1c	C	T	5: 120,808,061 (GRCm38)	S124N	probably benign	Het
Omg	T	A	11: 79,503,003 (GRCm38)	K10*	probably null	Het
Or2a52	A	G	6: 43,167,816 (GRCm38)	I253V	probably benign	Het
Or4k52	A	T	2: 111,780,841 (GRCm38)	I174F	probably damaging	Het
Or6c88	A	G	10: 129,571,177 (GRCm38)	H174R	probably damaging	Het
Pcdhb14	T	A	18: 37,449,639 (GRCm38)	N599K	probably damaging	Het
Pcdhb18	C	A	18: 37,490,647 (GRCm38)	N343K	probably damaging	Het
Phf7	C	A	14: 31,249,656 (GRCm38)		probably benign	Het
Pramel15	C	T	4: 144,372,827 (GRCm38)	C456Y	probably damaging	Het
Qdpr	T	C	5: 45,447,640 (GRCm38)	N42S	probably damaging	Het
Rbm44	T	A	1: 91,162,414 (GRCm38)	D716E	probably benign	Het
Rprd2	A	C	3: 95,764,055 (GRCm38)	H1345Q	possibly damaging	Het
Rsf1	G	GACGGCGGCT	7: 97,579,909 (GRCm38)		probably benign	Het
Ryr2	G	A	13: 11,799,882 (GRCm38)	A668V	probably damaging	Het
Sag	T	A	1: 87,831,961 (GRCm38)	L307Q	probably damaging	Het
Sash1	G	A	10: 8,727,970 (GRCm38)	P1106L	probably damaging	Het
Sec16b	T	A	1: 157,554,839 (GRCm38)	I615N	probably damaging	Het
Sfi1	A	ATCTTCCCAAAGCCAGTGC	11: 3,153,384 (GRCm38)		probably benign	Het
Slco1b2	T	C	6: 141,683,267 (GRCm38)	V600A	probably benign	Het
Sucnr1	A	G	3: 60,086,842 (GRCm38)	T264A	probably benign	Het
Tbx3	A	G	5: 119,671,918 (GRCm38)		probably benign	Het
Tigd5	T	C	15: 75,911,220 (GRCm38)	V477A	possibly damaging	Het
Tle7	T	A	8: 110,110,131 (GRCm38)	S216R	possibly damaging	Het
Ttc17	G	A	2: 94,362,419 (GRCm38)	A89V	probably damaging	Het
Twsg1	A	G	17: 65,948,662 (GRCm38)	I39T		Het
Zfp184	T	C	13: 21,959,342 (GRCm38)	F406S	probably damaging	Het
Zfp429	A	T	13: 67,390,711 (GRCm38)	C205S	probably damaging	Het
Zfp59	T	A	7: 27,854,888 (GRCm38)	D588E	probably benign	Het
Zfp64	A	T	2: 168,955,163 (GRCm38)	M1K	probably null	Het

Other mutations in Meioc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01391:Meioc	APN	11	102,674,287 (GRCm38)	missense	probably benign	0.33
IGL01952:Meioc	APN	11	102,672,185 (GRCm38)	missense	possibly damaging	0.79
IGL02006:Meioc	APN	11	102,674,266 (GRCm38)	missense	probably damaging	1.00
IGL02195:Meioc	APN	11	102,674,857 (GRCm38)	missense	possibly damaging	0.91
IGL02339:Meioc	APN	11	102,668,448 (GRCm38)	missense	probably benign	0.18
IGL02935:Meioc	APN	11	102,672,191 (GRCm38)	missense	probably benign	0.06
IGL03294:Meioc	APN	11	102,680,669 (GRCm38)	missense	probably damaging	1.00
PIT4519001:Meioc	UTSW	11	102,679,957 (GRCm38)	missense	probably damaging	1.00
R0285:Meioc	UTSW	11	102,672,191 (GRCm38)	missense	probably benign	0.06
R0964:Meioc	UTSW	11	102,680,031 (GRCm38)	missense	probably damaging	1.00
R1074:Meioc	UTSW	11	102,675,393 (GRCm38)	missense	probably damaging	1.00
R2024:Meioc	UTSW	11	102,675,358 (GRCm38)	missense	probably benign	0.00
R4012:Meioc	UTSW	11	102,675,828 (GRCm38)	missense	probably damaging	0.99
R4429:Meioc	UTSW	11	102,675,720 (GRCm38)	missense	probably damaging	1.00
R4491:Meioc	UTSW	11	102,674,920 (GRCm38)	missense	possibly damaging	0.84
R4594:Meioc	UTSW	11	102,674,166 (GRCm38)	missense	probably damaging	1.00
R4752:Meioc	UTSW	11	102,674,433 (GRCm38)	missense	probably benign	0.00
R5301:Meioc	UTSW	11	102,680,045 (GRCm38)	missense	probably damaging	1.00
R5352:Meioc	UTSW	11	102,675,313 (GRCm38)	missense	probably benign	0.03
R5646:Meioc	UTSW	11	102,675,257 (GRCm38)	missense	possibly damaging	0.94
R5958:Meioc	UTSW	11	102,675,153 (GRCm38)	missense	probably benign	0.41
R5968:Meioc	UTSW	11	102,675,831 (GRCm38)	missense	probably damaging	0.99
R6157:Meioc	UTSW	11	102,668,401 (GRCm38)	missense	probably damaging	1.00
R6410:Meioc	UTSW	11	102,675,034 (GRCm38)	missense	probably benign	0.00
R6644:Meioc	UTSW	11	102,668,460 (GRCm38)	critical splice donor site	probably null
R7285:Meioc	UTSW	11	102,666,342 (GRCm38)	missense	probably benign	0.00
R7440:Meioc	UTSW	11	102,674,237 (GRCm38)	missense	possibly damaging	0.67
R7815:Meioc	UTSW	11	102,675,588 (GRCm38)	missense	probably damaging	1.00
R7984:Meioc	UTSW	11	102,674,606 (GRCm38)	missense	possibly damaging	0.94
R8009:Meioc	UTSW	11	102,676,743 (GRCm38)	missense	probably damaging	1.00
R8078:Meioc	UTSW	11	102,668,400 (GRCm38)	nonsense	probably null
R8195:Meioc	UTSW	11	102,675,067 (GRCm38)	nonsense	probably null
R8429:Meioc	UTSW	11	102,674,206 (GRCm38)	missense	probably benign	0.06
R8797:Meioc	UTSW	11	102,676,860 (GRCm38)	nonsense	probably null
R8854:Meioc	UTSW	11	102,675,763 (GRCm38)	missense	probably damaging	0.98
R9081:Meioc	UTSW	11	102,674,175 (GRCm38)	missense	probably benign	0.00
R9360:Meioc	UTSW	11	102,674,953 (GRCm38)	missense	probably benign	0.13
R9539:Meioc	UTSW	11	102,674,680 (GRCm38)	missense	probably damaging	0.99
R9549:Meioc	UTSW	11	102,665,724 (GRCm38)	intron	probably benign
R9751:Meioc	UTSW	11	102,675,593 (GRCm38)	nonsense	probably null
Z1177:Meioc	UTSW	11	102,666,364 (GRCm38)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- AGAGTTCTTGAGTAGCTATCCCAAC -3'
(R):5'- TCCAGCACTCAAGAGATAAGGC -3'

Sequencing Primer
(F):5'- TGAGTAGCTATCCCAACTTCTAAC -3'
(R):5'- AGGTAACAAATATGAAGCCAACTTAC -3'

Posted On

2021-08-02