Mutagenetix > Incidental Mutation

Incidental Mutation 'R8894:Dock9'

677912

Institutional Source

Beutler Lab

Gene Symbol

Dock9

Ensembl Gene

ENSMUSG00000025558

Gene Name

dedicator of cytokinesis 9

Synonyms

D14Wsu89e, Zizimin1, B230309H04Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8894 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

121542046-121797837 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 121622961 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 850 (S850G)

Ref Sequence

ENSEMBL: ENSMUSP00000148834 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040700] [ENSMUST00000100299] [ENSMUST00000212181] [ENSMUST00000212376]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000040700
AA Change: S850G

PolyPhen 2 Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000047881
Gene: ENSMUSG00000025558
AA Change: S850G

Domain	Start	End	E-Value	Type
Pfam:DUF3398	58	151	5.6e-36	PFAM
PH	172	280	1.38e-16	SMART
Blast:PH	297	372	4e-25	BLAST
Pfam:DOCK-C2	631	822	5.3e-51	PFAM
Pfam:DHR-2	1523	2068	2.1e-212	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000100299
AA Change: S852G

PolyPhen 2 Score 0.120 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000097872
Gene: ENSMUSG00000025558
AA Change: S852G

Domain	Start	End	E-Value	Type
Pfam:DUF3398	58	153	1.5e-32	PFAM
PH	174	282	1.38e-16	SMART
Blast:PH	299	374	4e-25	BLAST
Pfam:DOCK-C2	632	825	1.3e-59	PFAM
low complexity region	1752	1763	N/A	INTRINSIC
Pfam:Ded_cyto	1836	2013	2.4e-69	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000212181
AA Change: S850G

PolyPhen 2 Score 0.101 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000212376
AA Change: S864G

PolyPhen 2 Score 0.223 (Sensitivity: 0.91; Specificity: 0.88)

Meta Mutation Damage Score

0.1222

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

98% (63/64)

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931429L15Rik	C	T	9: 46,305,099	V291M	probably benign	Het
Abca14	A	C	7: 120,248,145	Y744S	probably damaging	Het
Acly	T	C	11: 100,516,813	E237G	probably benign	Het
Adcy1	T	A	11: 7,137,375	D416E	probably damaging	Het
Arid1b	T	A	17: 5,327,393	M1217K	probably damaging	Het
Atad2b	T	C	12: 5,014,001		probably null	Het
Ccdc177	A	G	12: 80,759,303	S66P	probably damaging	Het
Clpsl2	T	C	17: 28,550,671	C36R	possibly damaging	Het
Cramp1l	C	T	17: 24,983,140	G456D	probably damaging	Het
Crb1	T	A	1: 139,248,012	L744F	possibly damaging	Het
Crybg3	T	A	16: 59,522,189	E980D	probably damaging	Het
Cyp2d11	A	T	15: 82,390,470	V302E	probably benign	Het
Cyp3a25	A	C	5: 145,994,860		probably benign	Het
Dhx35	G	C	2: 158,834,875	A410P	possibly damaging	Het
Dlg2	A	T	7: 91,965,738	H295L	probably benign	Het
Dnah2	T	A	11: 69,492,222	M1172L	probably benign	Het
Dst	A	T	1: 34,174,133	M1369L	possibly damaging	Het
Fam205a1	A	G	4: 42,853,688	C47R	possibly damaging	Het
Fcrl6	T	C	1: 172,599,289	N30S	probably benign	Het
Fibp	A	T	19: 5,463,281	Q208L	probably benign	Het
Foxc1	T	G	13: 31,808,222	S339A	unknown	Het
Fras1	T	C	5: 96,759,543	C3196R	probably damaging	Het
Galnt7	C	T	8: 57,526,142	W649*	probably null	Het
Gm7682	T	G	5: 94,448,540	C479G	probably damaging	Het
H2-M10.2	T	C	17: 36,284,663	N245S	possibly damaging	Het
Hapln3	G	A	7: 79,117,491	R267W	probably benign	Het
Hkdc1	T	C	10: 62,408,621	I229V	probably damaging	Het
Kank1	G	A	19: 25,431,014	G1286R	probably damaging	Het
Krba1	A	G	6: 48,411,695	I543V	probably damaging	Het
Lipn	A	T	19: 34,084,848	*401L	probably null	Het
Lrrc37a	T	C	11: 103,456,623	N3082S	unknown	Het
Magi1	A	T	6: 93,686,605	F1117Y	probably benign	Het
Man2a1	A	G	17: 64,713,601	K791E	probably benign	Het
Mindy4	T	C	6: 55,278,238	L567P	probably benign	Het
Mpp4	A	G	1: 59,158,584		probably null	Het
Muc2	T	G	7: 141,694,515	C371W	probably damaging	Het
Nebl	A	T	2: 17,375,225	N779K	probably benign	Het
Nf1	T	C	11: 79,445,793	L1066P	probably damaging	Het
Npat	T	C	9: 53,556,651	L302P	probably damaging	Het
Nudt21	C	A	8: 94,028,870	G146W	probably damaging	Het
Olfr1205	T	G	2: 88,831,465	M116R	probably benign	Het
Olfr122	T	A	17: 37,772,049	I132K	probably damaging	Het
Olfr1510	A	T	14: 52,410,008	L288Q	probably damaging	Het
Olfr891	A	T	9: 38,180,074	F250I	probably damaging	Het
Pkd2l2	T	C	18: 34,438,220		probably benign	Het
Plcd3	T	A	11: 103,071,766	N620Y	probably damaging	Het
Ppl	T	C	16: 5,107,342		probably benign	Het
Prep	T	C	10: 45,158,524	*711Q	probably null	Het
Psap	T	A	10: 60,299,957	V394E	possibly damaging	Het
Ptprr	T	A	10: 116,048,345	D102E	probably benign	Het
Rgsl1	T	A	1: 153,822,373		probably null	Het
Rimbp2	T	A	5: 128,773,390	D943V	possibly damaging	Het
Rsrc2	G	T	5: 123,740,730	R113S	unknown	Het
Sim1	T	A	10: 50,910,530	V286E	possibly damaging	Het
Slc35d1	A	T	4: 103,211,332	F124L		Het
Sort1	T	C	3: 108,338,912	F402L	probably damaging	Het
Tcrg-V4	T	C	13: 19,185,457	S124P	probably damaging	Het
Tlr2	T	A	3: 83,836,784	E664V	probably damaging	Het
Tmppe	A	T	9: 114,401,192		probably benign	Het
Tnfrsf8	T	C	4: 145,274,468	D356G	possibly damaging	Het
Tnrc18	T	C	5: 142,739,457	E1839G	unknown	Het
Trim17	T	A	11: 58,968,710	M250K	probably benign	Het
Trim33	C	T	3: 103,311,491	L315F	probably damaging	Het
Vmn1r43	A	G	6: 89,869,764	S247P	probably benign	Het
Vmn2r33	T	A	7: 7,563,810	Y101F	probably benign	Het
Vmn2r40	T	C	7: 8,920,198	D388G		Het
Wdr36	C	T	18: 32,837,287		probably benign	Het
Zfp2	T	A	11: 50,901,016	I67F	possibly damaging	Het

Other mutations in Dock9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00236:Dock9	APN	14	121668468	missense	probably benign	0.12
IGL00817:Dock9	APN	14	121698291	missense	probably damaging	0.96
IGL00923:Dock9	APN	14	121607092	unclassified	probably benign
IGL01385:Dock9	APN	14	121580583	missense	possibly damaging	0.94
IGL01567:Dock9	APN	14	121653084	missense	probably damaging	1.00
IGL01767:Dock9	APN	14	121622870	missense	possibly damaging	0.91
IGL01811:Dock9	APN	14	121559028	missense	probably damaging	1.00
IGL02512:Dock9	APN	14	121619538	splice site	probably benign
IGL02525:Dock9	APN	14	121640126	missense	probably damaging	1.00
IGL02550:Dock9	APN	14	121698312	start codon destroyed	probably null	0.07
IGL02559:Dock9	APN	14	121625147	splice site	probably benign
IGL02666:Dock9	APN	14	121580699	missense	probably benign	0.42
IGL02674:Dock9	APN	14	121595611	splice site	probably null
IGL02795:Dock9	APN	14	121639978	missense	probably benign	0.04
IGL03074:Dock9	APN	14	121607270	missense	possibly damaging	0.95
IGL03095:Dock9	APN	14	121639528	missense	probably damaging	1.00
IGL03294:Dock9	APN	14	121641623	splice site	probably benign
R0036:Dock9	UTSW	14	121622853	missense	probably damaging	1.00
R0050:Dock9	UTSW	14	121607225	missense	probably benign	0.43
R0050:Dock9	UTSW	14	121607225	missense	probably benign	0.43
R0164:Dock9	UTSW	14	121597665	missense	probably damaging	1.00
R0164:Dock9	UTSW	14	121597665	missense	probably damaging	1.00
R0270:Dock9	UTSW	14	121575999	missense	probably benign	0.02
R0494:Dock9	UTSW	14	121662584	missense	possibly damaging	0.64
R0726:Dock9	UTSW	14	121651768	nonsense	probably null
R1029:Dock9	UTSW	14	121599684	splice site	probably null
R1214:Dock9	UTSW	14	121586316	missense	probably benign	0.02
R1231:Dock9	UTSW	14	121575950	missense	possibly damaging	0.61
R1535:Dock9	UTSW	14	121546064	missense	probably damaging	1.00
R1629:Dock9	UTSW	14	121543574	missense	possibly damaging	0.88
R1637:Dock9	UTSW	14	121651775	missense	possibly damaging	0.66
R1733:Dock9	UTSW	14	121626880	missense	probably benign	0.01
R1772:Dock9	UTSW	14	121609798	missense	probably benign	0.07
R1855:Dock9	UTSW	14	121640159	missense	probably damaging	1.00
R1888:Dock9	UTSW	14	121625205	missense	probably benign	0.18
R1888:Dock9	UTSW	14	121625205	missense	probably benign	0.18
R1901:Dock9	UTSW	14	121625153	splice site	probably null
R1920:Dock9	UTSW	14	121583380	missense	probably damaging	1.00
R1987:Dock9	UTSW	14	121591830	missense	probably benign	0.00
R3035:Dock9	UTSW	14	121606837	missense	possibly damaging	0.60
R3851:Dock9	UTSW	14	121629086	splice site	probably null
R4020:Dock9	UTSW	14	121606855	missense	probably benign	0.00
R4021:Dock9	UTSW	14	121626912	missense	possibly damaging	0.80
R4089:Dock9	UTSW	14	121583471	missense	probably damaging	1.00
R4258:Dock9	UTSW	14	121581442	missense	probably benign	0.00
R4423:Dock9	UTSW	14	121562053	critical splice donor site	probably null
R4561:Dock9	UTSW	14	121559007	missense	probably benign	0.01
R4604:Dock9	UTSW	14	121668459	missense	probably damaging	1.00
R4646:Dock9	UTSW	14	121586246	missense	probably damaging	1.00
R4647:Dock9	UTSW	14	121586246	missense	probably damaging	1.00
R4776:Dock9	UTSW	14	121610097	missense	possibly damaging	0.81
R4809:Dock9	UTSW	14	121546596	missense	probably benign	0.37
R4865:Dock9	UTSW	14	121543505	makesense	probably null
R4951:Dock9	UTSW	14	121653135	missense	probably benign	0.35
R5151:Dock9	UTSW	14	121578170	missense	probably damaging	1.00
R5359:Dock9	UTSW	14	121653060	missense	possibly damaging	0.69
R5366:Dock9	UTSW	14	121578203	missense	probably damaging	1.00
R5502:Dock9	UTSW	14	121610182	splice site	probably null
R5579:Dock9	UTSW	14	121599695	missense	probably damaging	1.00
R5753:Dock9	UTSW	14	121634625	missense	probably benign	0.05
R5836:Dock9	UTSW	14	121681351	missense	probably damaging	1.00
R5858:Dock9	UTSW	14	121628792	missense	probably benign	0.00
R5890:Dock9	UTSW	14	121668408	critical splice donor site	probably null
R6075:Dock9	UTSW	14	121545973	missense	probably benign
R6298:Dock9	UTSW	14	121634594	missense	probably damaging	1.00
R6306:Dock9	UTSW	14	121562080	missense	probably damaging	1.00
R6321:Dock9	UTSW	14	121546021	missense	probably damaging	1.00
R6330:Dock9	UTSW	14	121605243	start codon destroyed	probably null	0.00
R6719:Dock9	UTSW	14	121610027	missense	probably damaging	1.00
R6784:Dock9	UTSW	14	121543514	missense	probably damaging	1.00
R6826:Dock9	UTSW	14	121622918	missense	probably damaging	1.00
R6830:Dock9	UTSW	14	121622918	missense	probably damaging	1.00
R6838:Dock9	UTSW	14	121546596	missense	possibly damaging	0.71
R6868:Dock9	UTSW	14	121586264	missense	probably benign	0.37
R6919:Dock9	UTSW	14	121643152	missense	probably benign	0.42
R6989:Dock9	UTSW	14	121627379	missense	probably damaging	1.00
R7539:Dock9	UTSW	14	121581436	missense	probably damaging	1.00
R7645:Dock9	UTSW	14	121597663	missense	probably benign	0.44
R7875:Dock9	UTSW	14	121625984	nonsense	probably null
R7900:Dock9	UTSW	14	121546079	missense	possibly damaging	0.84
R8040:Dock9	UTSW	14	121651794	missense	probably benign	0.06
R8420:Dock9	UTSW	14	121546042	missense	probably damaging	1.00
R8511:Dock9	UTSW	14	121627389	missense	probably benign	0.40
R8511:Dock9	UTSW	14	121681435	missense	probably damaging	1.00
R8514:Dock9	UTSW	14	121658787	missense	probably benign	0.25
R8691:Dock9	UTSW	14	121640105	missense	possibly damaging	0.49
R8804:Dock9	UTSW	14	121605183	missense	probably damaging	0.98
R8900:Dock9	UTSW	14	121580528	missense	probably damaging	1.00
R9069:Dock9	UTSW	14	121628912	missense	probably damaging	0.98
R9218:Dock9	UTSW	14	121668459	missense	probably damaging	1.00
R9233:Dock9	UTSW	14	121583369	missense	probably benign	0.09
R9236:Dock9	UTSW	14	121639558	missense	probably damaging	1.00
R9285:Dock9	UTSW	14	121595600	missense	probably benign
R9451:Dock9	UTSW	14	121550189	splice site	probably benign
R9461:Dock9	UTSW	14	121605189	missense	probably benign	0.05
R9484:Dock9	UTSW	14	121581432	missense	probably damaging	1.00
R9517:Dock9	UTSW	14	121591824	missense	probably benign	0.07
R9542:Dock9	UTSW	14	121627363	missense	probably damaging	1.00
R9694:Dock9	UTSW	14	121581379	missense	probably damaging	1.00
R9701:Dock9	UTSW	14	121639571	missense	probably benign	0.01
R9703:Dock9	UTSW	14	121544577	makesense	probably null
R9726:Dock9	UTSW	14	121597737	missense	possibly damaging	0.61
R9741:Dock9	UTSW	14	121640104	missense	probably damaging	1.00
Z1088:Dock9	UTSW	14	121555275	missense	probably damaging	1.00
Z1176:Dock9	UTSW	14	121651782	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TTTCAGAGAGGCTCCAGGTGTG -3'
(R):5'- GAGGGAGTTCTTCTATCCTTCCTTG -3'

Sequencing Primer
(F):5'- TCCAGGTGTGGACAGACTG -3'
(R):5'- CATGGTAATGTGAACCTGTTGACCC -3'

Posted On

2021-08-02